Gene Details:

• MSU gene ID: LOC_Os06g37364
• RAPdb gene ID: Os06g0570100
• Gene Symbol: D35 OsKOS3 OsKO2
• Genome: MSU7 ,  IRGSP-1.0
• Species: Oryza sativa

Functional Descriptions:

• ) semi-dwarf cultivar, Tan-Ginbozu (D35Tan-Ginbozu), contributed to the increase in crop productivity in Japan in the 1950s.
• Previous studies suggested that the semi-dwarf stature of D35Tan-Ginbozu is caused by a defective early step of gibberellin biosynthesis, which is catalyzed by ent-kaurene oxidase (KO).
• Homozygote with null alleles of D35 showed a severe dwarf phenotype; therefore, D35Tan-Ginbozu is a weak allele of D35.
• Introduction of OsKOL4 into D35Tan-Ginbozu did not rescue its dwarf phenotype, indicating that OsKOL4 is not involved in GA biosynthesis.
• A rice semi-dwarf gene, Tan-Ginbozu (D35), encodes the gibberellin biosynthesis enzyme, ent-kaurene oxidase.
• Comparing D35Tan-Ginbozu with other high yielding cultivars, we discuss strategies to produce culm architectures suitable for high crop yield by decreasing GA levels.
• Rice (Oryza sativa) contains five CYP701A subfamily members in its genome, despite the fact that only one (OsKO2/CYP701A6) is required for GA biosynthesis.
• In particular, under conditions where OsKO2 catalyzes the expected conversion of ent-kaurene to ent-kaurenoic acid required for GA biosynthesis, OsKOL4 instead efficiently reacts with ent-sandaracopimaradiene and ent-cassadiene to produce the corresponding C3alpha-hydroxylated diterpenoids.
• Rice ent-kaurene oxidase 2 (OsKO2) perhaps functions in the early steps of gibberellin biosynthesis.
• This is direct evidence that OsKO2 is involved in the sequential oxidation of ent-kaurene to ent-kaurenoic acid in gibberellin biosynthesis in rice.

Function-related keywords:

• dwarf ,  gibberellin ,  architecture ,  culm ,  ga ,  yield

Literature:

• A rice semi-dwarf gene, Tan-Ginbozu (D35), encodes the gibberellin biosynthesis enzyme, ent-kaurene oxidase .  DOI:  10.1023/B:PLAN.0000038261.21060.47 ;  PMID:  15316288
• Functional identification of a rice ent-kaurene oxidase, OsKO2, using the Pichia pastoris expression system .  DOI:  10.1271/bbb.80427 ;  PMID:  19060383
• Mutation of rice BC12/GDD1, which encodes a kinesin-like protein that binds to a GA biosynthesis gene promoter, leads to dwarfism with impaired cell elongation .  DOI:  10.1105/tpc.110.081901 ;  PMID:  21325138
• The rice dwarf virus P2 protein interacts with ent-kaurene oxidases in vivo, leading to reduced biosynthesis of gibberellins and rice dwarf symptoms .  DOI:  10.1104/pp.105.072306 ;  PMID:  16299167
• CYP701A8: a rice ent-kaurene oxidase paralog diverted to more specialized diterpenoid metabolism .  DOI:  10.1104/pp.111.187518 ;  PMID:  22247270
• Overexpression of rice LRK1 restricts internode elongation by down-regulating OsKO2 .  DOI:  10.1007/s10529-012-1054-9 ;  PMID:  23007447

Related News:

Gene Resources:

• NCBI ID: AK066285  , AY660665
• UniProt accessions:

Sequences: