Gene Details:
- MSU gene ID: LOC_Os06g37330
- RAPdb gene ID: Os06g0569900
- Gene Symbol: OsKOS4 OsKO1
- Genome: MSU7 , IRGSP-1.0
- Species: Oryza sativa
Functional Descriptions:
- The expression levels of gibberellin (GA) biosynthetic genes including OsCPS1, OsKS1, OsKO1, OsKAO, OsGA20ox2/SD1 and OsGA2ox3 were significantly increased in d62 mutant.
- Further analyses proved that OsKO1 could catalyze the reaction from ent-kaurene into ent-kaurenoic acid in GA biosynthesis mainly at seed germination and seedling stages, and the mutations decrease its activity to catalyze the step from ent-kaurenol to ent-kaurenoic acid in this reaction.
- Genetic complementary analysis and enzyme assay verified that the mutations in OsKO1 gene block the biosynthesis of GA and result in the defect phenotypes.
Function-related keywords:
- gibberellin , seedling , seed , seed-germination , ga , GA , GA-biosynthesis
Literature:
- Identification and characterization of dwarf 62, a loss-of-function mutation in DLT/OsGRAS-32 affecting gibberellin metabolism in rice . DOI: 10.1007/s00425-010-1263-1 ; PMID: 20830595
- The rice dwarf virus P2 protein interacts with ent-kaurene oxidases in vivo, leading to reduced biosynthesis of gibberellins and rice dwarf symptoms . DOI: 10.1104/pp.105.072306 ; PMID: 16299167
- Mutations on ent-kaurene oxidase 1 encoding gene attenuate its enzyme activity of catalyzing the reaction from ent-kaurene to ent-kaurenoic acid and lead to delayed germination in rice . DOI: 10.1371/journal.pgen.1008562 ; PMID: 31923187
Related News:
Gene Resources:
Sequences:
cDNA Sequence
- >LOC_Os06g37330.1
ATCAAGAGAATGCAGTGCAAGTGCAAACTCTCGCAGTGAGCCTGTGTGATCGATCGATCTGAGTAGGGGTCGGCCATGGAGTCGCTGCTCGCAGCCGGTGCGGGAGGGATCGGTGTGGCGGCGGCGGCCGTCGGGGGGTTTATCGCGGCGGCGACACTCGCCGTGGCACCGCCCAAGAACCGCCGCAACCCGCCTCCAGCTGTTCCTGGTTTACCCATAATCGGAAATCTGCATCAATTGAAAGAAAAGAAGCCTCATCAGACCTTCACAAAATGGGCTGAAATTTATGGCCCAATCTACACTATAAGGACCGGGGCTTCTTCCGTAGTTGTGCTCAATTCAACTGAAGTAGCCAAGGAGGCGATGGTTGCAAAATTCTCATCCATATCTACCCGAAAGCTATCCAAAGCACTAACAGTGCTTAGTCATGATAAATCCATGGTTGCTACCAGCGACAGTGGTGATTTCCACAAAATGGGGAAGCGTTATATCATGTTAAGCATGCTGGGTACTTCTGCACAGAAACAATTTCGTGACACAAGAGATATGATCATCAATAACATGTTAAGCACTTTCCATCAACTGGTGAAAGATGACCCACATGCTCCTCTGATATTCAGAGATGTTTTCAAGGATGAGCTATTCCGGTTGTCCATGATCCAGAGCTTAGGAGAGGATGTGAGTTCAGTCTATGTGGATGAATTTGGGAGGGACATTTCGAAGGAAGAAATCTACAATGCTACTGTGACTGACATGATGATGTGCGCAATTGAGGTCGACTGGAGAGATTTCTTCCCCTACCTCAGCTGGGTTCCAAACAAGAGCTTCGAAACAAGAGTGTTTACTACAGAATCTAGACGAACTGCGGTGATGCGCGCCTTGATCAAGCAGCAGAAGGAAAGGATTGTGCGTGGAGAGGCAAGGACATGCTATCTGGACTTCTTGCTGGCAGAGAACACACTGACAGATGAGCAACTAATGATGCTAGTGTGGGAGGCACTCATAGAGGCTGCAGATACTACCTTGGTCACCACAGAATGGGCCATGTATGAGCTTGCCAAGAACCCTGACAAACAGGAACGGCTTTACCAAGAGATCCGGGAGGTGTGCGGCGACGAGGCGGTCACCGAGGAGCACCTGCCGTGGCTGCCGTACCTCAATGCCGTCTTCCAGGAGACGCTGCGCCGCCACTCCCCTGTCCCGCTCATACCTCCGAGGTTCGTCAACGAGGACACCATGCTCGCCGGCTACGATGTCCCCGCCGGCACCGAGATGGTGATCAACCTGTATGGGTGCAACATGAACAAGAAGGAGTGGGAGTCGCCGGAGGAGTGGGCGCCGGAGAGGTTCGCCGGTGGGAGGTTCAAGGTGGCGGACATGTACAAGACGATGGCGTTCGGCGCCGGGAGGAGGGTCTGCGCAGGGAGCCTGCAGGCGACTCACATCGCGTGCGCCGCCATCGCGCGCTTCGTGCAGGAGTTTGGGTGGAGGCTGAGGGAGGGTGACGAGGAGAAGGTGGACACCGTGCAGCTCACCGCCTACAAGCTCCACCCGCTTCATGTCCACCTCACGCCCAGAGGAAGGATGTGAACCATTATATTCATTGGAAGCTGTTGCTCGTTGAATAATGATATATAGTTATCTTTGAGATGTATTTACTATTAGCAAAATCTCCATGCTTTGTTCTGGATAGGAGAAAACATATTATAATATATCGTGGAATATTTTTTTTATC
CDS Sequence
- >LOC_Os06g37330.1
ATGGAGTCGCTGCTCGCAGCCGGTGCGGGAGGGATCGGTGTGGCGGCGGCGGCCGTCGGGGGGTTTATCGCGGCGGCGACACTCGCCGTGGCACCGCCCAAGAACCGCCGCAACCCGCCTCCAGCTGTTCCTGGTTTACCCATAATCGGAAATCTGCATCAATTGAAAGAAAAGAAGCCTCATCAGACCTTCACAAAATGGGCTGAAATTTATGGCCCAATCTACACTATAAGGACCGGGGCTTCTTCCGTAGTTGTGCTCAATTCAACTGAAGTAGCCAAGGAGGCGATGGTTGCAAAATTCTCATCCATATCTACCCGAAAGCTATCCAAAGCACTAACAGTGCTTAGTCATGATAAATCCATGGTTGCTACCAGCGACAGTGGTGATTTCCACAAAATGGGGAAGCGTTATATCATGTTAAGCATGCTGGGTACTTCTGCACAGAAACAATTTCGTGACACAAGAGATATGATCATCAATAACATGTTAAGCACTTTCCATCAACTGGTGAAAGATGACCCACATGCTCCTCTGATATTCAGAGATGTTTTCAAGGATGAGCTATTCCGGTTGTCCATGATCCAGAGCTTAGGAGAGGATGTGAGTTCAGTCTATGTGGATGAATTTGGGAGGGACATTTCGAAGGAAGAAATCTACAATGCTACTGTGACTGACATGATGATGTGCGCAATTGAGGTCGACTGGAGAGATTTCTTCCCCTACCTCAGCTGGGTTCCAAACAAGAGCTTCGAAACAAGAGTGTTTACTACAGAATCTAGACGAACTGCGGTGATGCGCGCCTTGATCAAGCAGCAGAAGGAAAGGATTGTGCGTGGAGAGGCAAGGACATGCTATCTGGACTTCTTGCTGGCAGAGAACACACTGACAGATGAGCAACTAATGATGCTAGTGTGGGAGGCACTCATAGAGGCTGCAGATACTACCTTGGTCACCACAGAATGGGCCATGTATGAGCTTGCCAAGAACCCTGACAAACAGGAACGGCTTTACCAAGAGATCCGGGAGGTGTGCGGCGACGAGGCGGTCACCGAGGAGCACCTGCCGTGGCTGCCGTACCTCAATGCCGTCTTCCAGGAGACGCTGCGCCGCCACTCCCCTGTCCCGCTCATACCTCCGAGGTTCGTCAACGAGGACACCATGCTCGCCGGCTACGATGTCCCCGCCGGCACCGAGATGGTGATCAACCTGTATGGGTGCAACATGAACAAGAAGGAGTGGGAGTCGCCGGAGGAGTGGGCGCCGGAGAGGTTCGCCGGTGGGAGGTTCAAGGTGGCGGACATGTACAAGACGATGGCGTTCGGCGCCGGGAGGAGGGTCTGCGCAGGGAGCCTGCAGGCGACTCACATCGCGTGCGCCGCCATCGCGCGCTTCGTGCAGGAGTTTGGGTGGAGGCTGAGGGAGGGTGACGAGGAGAAGGTGGACACCGTGCAGCTCACCGCCTACAAGCTCCACCCGCTTCATGTCCACCTCACGCCCAGAGGAAGGATGTGA
Protein Sequence
- >LOC_Os06g37330.1
MESLLAAGAGGIGVAAAAVGGFIAAATLAVAPPKNRRNPPPAVPGLPIIGNLHQLKEKKPHQTFTKWAEIYGPIYTIRTGASSVVVLNSTEVAKEAMVAKFSSISTRKLSKALTVLSHDKSMVATSDSGDFHKMGKRYIMLSMLGTSAQKQFRDTRDMIINNMLSTFHQLVKDDPHAPLIFRDVFKDELFRLSMIQSLGEDVSSVYVDEFGRDISKEEIYNATVTDMMMCAIEVDWRDFFPYLSWVPNKSFETRVFTTESRRTAVMRALIKQQKERIVRGEARTCYLDFLLAENTLTDEQLMMLVWEALIEAADTTLVTTEWAMYELAKNPDKQERLYQEIREVCGDEAVTEEHLPWLPYLNAVFQETLRRHSPVPLIPPRFVNEDTMLAGYDVPAGTEMVINLYGCNMNKKEWESPEEWAPERFAGGRFKVADMYKTMAFGAGRRVCAGSLQATHIACAAIARFVQEFGWRLREGDEEKVDTVQLTAYKLHPLHVHLTPRGRM*