Information report for OsKOS1;OsKOL4
Gene Details
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Functional Descriptions
- Introduction of OsKOL4 into d35Tan-Ginbozu did not rescue its dwarf phenotype, indicating that OsKOL4 is not involved in GA biosynthesis.
- In particular, under conditions where OsKO2 catalyzes the expected conversion of ent-kaurene to ent-kaurenoic acid required for GA biosynthesis, OsKOL4 instead efficiently reacts with ent-sandaracopimaradiene and ent-cassadiene to produce the corresponding C3alpha-hydroxylated diterpenoids.
- Thus, it appears that OsKOL4 plays a role in the more specialized diterpenoid metabolism of rice, and our results provide evidence for divergence of a KO/CYP701 family member from GA biosynthesis.
- Gibberellin responsiveness study and quantitative real-time PCR showed a faulty gibberellin pathway and epistatic control between the genes such as OsKOL4 and OsBRD2 causing semi-dwarfism in a mutant.
Functional Keywords
- dwarf , ga , gibberellin , Gibberellin
Literature and News
- A rice semi-dwarf gene, Tan-Ginbozu (D35), encodes the gibberellin biosynthesis enzyme, ent-kaurene oxidase . DOI: 10.1023/B:PLAN.0000038261.21060.47 ; PMID: 15316288
- The rice dwarf virus P2 protein interacts with ent-kaurene oxidases in vivo, leading to reduced biosynthesis of gibberellins and rice dwarf symptoms . DOI: 10.1104/pp.105.072306 ; PMID: 16299167
- CYP701A8: a rice ent-kaurene oxidase paralog diverted to more specialized diterpenoid metabolism . DOI: 10.1104/pp.111.187518 ; PMID: 22247270
- Optimization of recombinant expression enables discovery of novel cytochrome P450 activity in rice diterpenoid biosynthesis . DOI: 10.1007/s00253-015-6496-2 ; PMID: 25758958
- Assessment of efficacy of mutagenesis of gamma-irradiation in plant height and days to maturity through expression analysis in rice . DOI: 10.1371/journal.pone.0245603 ; PMID: 33449977
- Uncovering the Novel QTLs and Candidate Genes of Salt Tolerance in Rice with Linkage Mapping, RTM-GWAS, and RNA-seq . DOI: 10.1186/s12284-021-00535-3 ; PMID: 34778931
Gene Resources
- NCBI ID: AY579214
Sequences
cDNA Sequence
- >LOC_Os06g37300.2
GTGCGTGTGATCAGATCGTGTGCCATGGAGTCGATGCTCGTAGCCGGAGCGGGCGCGGCGGCGGTGGCGGCCGTCGGGGGCCTCGTCGCGGCGGCCGCGCTCGCCGACAAGCTCGTCGCGGCGCCGCCGCCGCGCAAGAACCGCGCCAACCCGCCTCCAGCTGTTCCTGGTTTACCCATTATTGGAAATCTGCATCAATTGAAAGAAAAGAAGCCTCATCAGACGTTTGCAAAATGGTCTGAAACTTATGGACCAATCTACACTATAAAGACCGGAGCTTCTCCAGTGGTTGTGCTCAATTCAACTGAAGTAGCCAAGGAGGCGATGATTGACAAATTCTCATCCATATCTACTCGAAAGCTACCAAAAGCAATGTCTGTGCTAACTCGTAAAAGTATGGTCGCAATCAGCGACTACGGTGACTACCAAAAGATGGCGAAGCGTAATATTATGATTGGCATGTTAGGTTTTAATGCACAGAAACAGTTTCGCGGTACAAGAGAGAGGATGATCAGTAACGTGTTAAGCACTTTGCATAAGTTGGTTTCTCTTGACCCACATTCCCCTCTGAACTTCAGGGATGTTTACATTAATGAGCTGTTCAGCTTGTCCTTGATCCAGAGTTTAGGTGAGGATGTGAGTTCAGTTTATGTGGAAGAGTTTGGGAGGGAGATATCCAAGGACGAAATCTTTGATGTCCTTGTGCATGAGATGATGATGTGTGCAGTTGAGGCTGACTGGAGGGACTACTTCCCCTACCTCAGCTGGCTTCCAAACAAGAGCTTCGACACAATTGTGTCTACTACAGAATTCAGACGAGATGCTATCATGAATGCATTGATCAAGAAGCAGAAGGAGAGGATTGCACGCGGAGAGGTGACACAAAACTTCTATTCGTATCCAAAAATAACTTTTTTTTTCTGCAGATCATCTGAACTGAACTGTAACGTATTGCATGCAGGCAAGGGCATCCTACATTGACTTCTTGCTGGAAGCTGAGAGGAGTGCACAGCTGACAGATGACCAACTGATGCTGCTGCTGTCGGAGTCCATCCTGGCTGCAGCTGATACTGTCCTGGTGACCACCGAATGGACCATGTATGAGATTGCCAAGAACCCTGACAAACAGGAGCTACTCTACCAAGAGATCCGAGAGGCGTGCGGCGGCGAGGCGGTGACCGAGGACGACTTGCCGCGGCTGCCGTACCTCAACGCCGTGTTCCACGAGACGCTGCGGCTGCACTCCCCGGTGCCGGTGCTGCCCCCGAGGTTCGTCCACGACGACACCACGCTCGCCGGCTACGACATCGCGGCGGGCACCCAGATGATGATCAACGTGTACGCGTGCCACATGGACGAGAAGGTGTGGGAGTCGCCGGGGGAGTGGTCGCCGGAGAGGTTCCTCGGCGAGGGGTTCGAGGTGGCGGACAGGTACAAGACGATGGCGTTCGGCGCCGGGAGGAGGACCTGCGCGGGGAGCCTGCAGGCGATGAACATCGCGTGCGTCGCCGTGGCGCGCCTCGTGCAGGAGCTCGAGTGGAGGCTGAGGGAGGGCGACGGGGACAAGGAGGACACCATGCAGTTCACCGCCTTGAAGCTTGACCCGCTGCATGTCCACCTCAAGCCCAGAGGAAGGATGTGAGCTAGAAGAAGCAGTTCCATGCATGATGAAGAATTAATGATATTCTCGAACGTATTTTGCAGGATGAATAGTGATATTAAAAATATAATGGTATATACTACCTCTGGTTTTTTAATATGCTAGAAAAAAATGTATGTGCACTGCTAGGGGAGAAATTAAATTTATCATGAACATCGTAGTATTTTTTTTTAAAATGTACGACGCCAACGTTTGACCATTCGTCCCTTATATATTCAATGGTACAATTTAGATTTAATCAAATAGAGAAAAAAAGCATTACACCTCTCC - >LOC_Os06g37300.1
GTGCGTGTGATCAGATCGTGTGCCATGGAGTCGATGCTCGTAGCCGGAGCGGGCGCGGCGGCGGTGGCGGCCGTCGGGGGCCTCGTCGCGGCGGCCGCGCTCGCCGACAAGCTCGTCGCGGCGCCGCCGCCGCGCAAGAACCGCGCCAACCCGCCTCCAGCTGTTCCTGGTTTACCCATTATTGGAAATCTGCATCAATTGAAAGAAAAGAAGCCTCATCAGACGTTTGCAAAATGGTCTGAAACTTATGGACCAATCTACACTATAAAGACCGGAGCTTCTCCAGTGGTTGTGCTCAATTCAACTGAAGTAGCCAAGGAGGCGATGATTGACAAATTCTCATCCATATCTACTCGAAAGCTACCAAAAGCAATGTCTGTGCTAACTCGTAAAAGTATGGTCGCAATCAGCGACTACGGTGACTACCAAAAGATGGCGAAGCGTAATATTATGATTGGCATGTTAGGTTTTAATGCACAGAAACAGTTTCGCGGTACAAGAGAGAGGATGATCAGTAACGTGTTAAGCACTTTGCATAAGTTGGTTTCTCTTGACCCACATTCCCCTCTGAACTTCAGGGATGTTTACATTAATGAGCTGTTCAGCTTGTCCTTGATCCAGAGTTTAGGTGAGGATGTGAGTTCAGTTTATGTGGAAGAGTTTGGGAGGGAGATATCCAAGGACGAAATCTTTGATGTCCTTGTGCATGAGATGATGATGTGTGCAGTTGAGGCTGACTGGAGGGACTACTTCCCCTACCTCAGCTGGCTTCCAAACAAGAGCTTCGACACAATTGTGTCTACTACAGAATTCAGACGAGATGCTATCATGAATGCATTGATCAAGAAGCAGAAGGAGAGGATTGCACGCGGAGAGGTGACACAAAACTTCTATTCGTATCCAAAAATAACTTTTTTTTTCTGCAGATCATCTGAACTGAACTGTAACGTATTGCATGCAGGCAAGGGCATCCTACATTGACTTCTTGCTGGAAGCTGAGAGGAGTGCACAGCTGACAGATGACCAACTGATGCTGCTGCTGTCGGAGTCCATCCTGGCTGCAGCTGATACTGTCCTGGTGACCACCGAATGGACCATGTATGAGATTGCCAAGAACCCTGACAAACAGGAGCTACTCTACCAAGAGATCCGAGAGGCGTGCGGCGGCGAGGCGGTGACCGAGGACGACTTGCCGCGGCTGCCGTACCTCAACGCCGTGTTCCACGAGACGCTGCGGCTGCACTCCCCGGTGCCGGTGCTGCCCCCGAGGTTCGTCCACGACGACACCACGCTCGCCGGCTACGACATCGCGGCGGGCACCCAGATGATGATCAACGTGTACGCGTGCCACATGGACGAGAAGGTGTGGGAGTCGCCGGGGGAGTGGTCGCCGGAGAGGTTCCTCGGCGAGGGGTTCGAGGTGGCGGACAGGTACAAGACGATGGCGTTCGGCGCCGGGAGGAGGACCTGCGCGGGGAGCCTGCAGGCGATGAACATCGCGTGCGTCGCCGTGGCGCGCCTCGTGCAGGAGCTCGAGTGGAGGCTGAGGGAGGGCGACGGGGACAAGGAGGACACCATGCAGTTCACCGCCTTGAAGCTTGACCCGCTGCATGTCCACCTCAAGCCCAGAGGAAGGATGTGAGCTAGAAGAAGCAGTTCCATGCATGATGAAGAATTAATGATATTCTCGAACGTATTTTGCAGGATGAATAGTGATATTAAAAATATAATGGTATATACTACCTCTGGTTTTTTAATATGCTAGAAAAAAATGTATGTGCACTGCTAGGGGAGAAATTAAATTTATCATGAACATCGTAGTATTTTTTTTTAAAATGTACGACGCCAACGTTTGACCATTCGTCCCTTATATATTCAATGGTACAATTTAGATTTAATCAAATAGAGAAAAAAAGCATTACACCTCTCC
CDS Sequence
- >LOC_Os06g37300.2
ATGGAGTCGATGCTCGTAGCCGGAGCGGGCGCGGCGGCGGTGGCGGCCGTCGGGGGCCTCGTCGCGGCGGCCGCGCTCGCCGACAAGCTCGTCGCGGCGCCGCCGCCGCGCAAGAACCGCGCCAACCCGCCTCCAGCTGTTCCTGGTTTACCCATTATTGGAAATCTGCATCAATTGAAAGAAAAGAAGCCTCATCAGACGTTTGCAAAATGGTCTGAAACTTATGGACCAATCTACACTATAAAGACCGGAGCTTCTCCAGTGGTTGTGCTCAATTCAACTGAAGTAGCCAAGGAGGCGATGATTGACAAATTCTCATCCATATCTACTCGAAAGCTACCAAAAGCAATGTCTGTGCTAACTCGTAAAAGTATGGTCGCAATCAGCGACTACGGTGACTACCAAAAGATGGCGAAGCGTAATATTATGATTGGCATGTTAGGTTTTAATGCACAGAAACAGTTTCGCGGTACAAGAGAGAGGATGATCAGTAACGTGTTAAGCACTTTGCATAAGTTGGTTTCTCTTGACCCACATTCCCCTCTGAACTTCAGGGATGTTTACATTAATGAGCTGTTCAGCTTGTCCTTGATCCAGAGTTTAGGTGAGGATGTGAGTTCAGTTTATGTGGAAGAGTTTGGGAGGGAGATATCCAAGGACGAAATCTTTGATGTCCTTGTGCATGAGATGATGATGTGTGCAGTTGAGGCTGACTGGAGGGACTACTTCCCCTACCTCAGCTGGCTTCCAAACAAGAGCTTCGACACAATTGTGTCTACTACAGAATTCAGACGAGATGCTATCATGAATGCATTGATCAAGAAGCAGAAGGAGAGGATTGCACGCGGAGAGGTGACACAAAACTTCTATTCGTATCCAAAAATAACTTTTTTTTTCTGCAGATCATCTGAACTGAACTGTAACGTATTGCATGCAGGCAAGGGCATCCTACATTGA - >LOC_Os06g37300.1
ATGGAGTCGATGCTCGTAGCCGGAGCGGGCGCGGCGGCGGTGGCGGCCGTCGGGGGCCTCGTCGCGGCGGCCGCGCTCGCCGACAAGCTCGTCGCGGCGCCGCCGCCGCGCAAGAACCGCGCCAACCCGCCTCCAGCTGTTCCTGGTTTACCCATTATTGGAAATCTGCATCAATTGAAAGAAAAGAAGCCTCATCAGACGTTTGCAAAATGGTCTGAAACTTATGGACCAATCTACACTATAAAGACCGGAGCTTCTCCAGTGGTTGTGCTCAATTCAACTGAAGTAGCCAAGGAGGCGATGATTGACAAATTCTCATCCATATCTACTCGAAAGCTACCAAAAGCAATGTCTGTGCTAACTCGTAAAAGTATGGTCGCAATCAGCGACTACGGTGACTACCAAAAGATGGCGAAGCGTAATATTATGATTGGCATGTTAGGTTTTAATGCACAGAAACAGTTTCGCGGTACAAGAGAGAGGATGATCAGTAACGTGTTAAGCACTTTGCATAAGTTGGTTTCTCTTGACCCACATTCCCCTCTGAACTTCAGGGATGTTTACATTAATGAGCTGTTCAGCTTGTCCTTGATCCAGAGTTTAGGTGAGGATGTGAGTTCAGTTTATGTGGAAGAGTTTGGGAGGGAGATATCCAAGGACGAAATCTTTGATGTCCTTGTGCATGAGATGATGATGTGTGCAGTTGAGGCTGACTGGAGGGACTACTTCCCCTACCTCAGCTGGCTTCCAAACAAGAGCTTCGACACAATTGTGTCTACTACAGAATTCAGACGAGATGCTATCATGAATGCATTGATCAAGAAGCAGAAGGAGAGGATTGCACGCGGAGAGGTGACACAAAACTTCTATTCGTATCCAAAAATAACTTTTTTTTTCTGCAGATCATCTGAACTGAACTGTAACGTATTGCATGCAGGCAAGGGCATCCTACATTGA
Protein Sequence
- >LOC_Os06g37300.2
MESMLVAGAGAAAVAAVGGLVAAAALADKLVAAPPPRKNRANPPPAVPGLPIIGNLHQLKEKKPHQTFAKWSETYGPIYTIKTGASPVVVLNSTEVAKEAMIDKFSSISTRKLPKAMSVLTRKSMVAISDYGDYQKMAKRNIMIGMLGFNAQKQFRGTRERMISNVLSTLHKLVSLDPHSPLNFRDVYINELFSLSLIQSLGEDVSSVYVEEFGREISKDEIFDVLVHEMMMCAVEADWRDYFPYLSWLPNKSFDTIVSTTEFRRDAIMNALIKKQKERIARGEVTQNFYSYPKITFFFCRSSELNCNVLHAGKGILH* - >LOC_Os06g37300.1
MESMLVAGAGAAAVAAVGGLVAAAALADKLVAAPPPRKNRANPPPAVPGLPIIGNLHQLKEKKPHQTFAKWSETYGPIYTIKTGASPVVVLNSTEVAKEAMIDKFSSISTRKLPKAMSVLTRKSMVAISDYGDYQKMAKRNIMIGMLGFNAQKQFRGTRERMISNVLSTLHKLVSLDPHSPLNFRDVYINELFSLSLIQSLGEDVSSVYVEEFGREISKDEIFDVLVHEMMMCAVEADWRDYFPYLSWLPNKSFDTIVSTTEFRRDAIMNALIKKQKERIARGEVTQNFYSYPKITFFFCRSSELNCNVLHAGKGILH*