Gene Details:

Functional Descriptions:

  • Disruption of a rice gene for alpha-glucan water dikinase, OsGWD1, leads to hyperaccumulation of starch in leaves but exhibits limited effects on growth.
  • Analysis of the GWD1 plants indicated that promoter editing mediated down-regulation of GWD1 caused no observable effects on rice growth and development, but only mildly modified its grain transparency and seed germination.
  • Therefore, our study shows that GWD1 is not only involved in transitory starch degradation in source tissues, but also plays key roles in the seeds, which is a sink tissue.
  • In conclusion, we find that GWD1 is an ideal biotechnological target with promising potential for the breeding of elite rice cultivars via genetic engineering.

Literature:

Gene Resources:

  • NCBI ID:
  • UniProt accessions:

Sequences:

cDNA Sequence
  • >LOC_Os06g30310.1
    ATGGCGCTAAGGAAGGAGAGATATCCATCCGGATCCCGGAAGCCGAATCCATCCATCCATCCATCCCATACTGCCCTTACGATCGAGCTGTTTGATATTCGTGCAGATGAGCGGATTCTCCGCGGCAGCTGCTGCGGCCGAGCGCTTGTCGGAAGGTTCACCCTGGATGCCAACTCCGAGCTTAAGGTGACATTGAACCCAGCACCGCAGGGTTCGGTGGTGGAGATCAATCTAGAGGCAACTAACACCAGCGGCTCCCTGATACTGCATTGGGGCGCCCTTCGCCCGGATAGAGGAGAATGGCTCCTACCATCCCGGAAACCAGATGGCACGACAGTGTACAAGAACAGGGCTCTTAGGACGCCTTTTATAAAGTCAGGTGATAACTCCACGCTGAAAATTGAGATAGATGATCCTGCAGTGCAAGCCATTGAGTTCCTCATATTTGATGAGGCACGGAATAATTGGTCAGTTCTTTTTGGGTTACCCATCCTACCCTGTTTCTTGGAATGCAGAAGATATCAAATAACAACACTCTACTTTGGACAGGAGGAGTATGAAGCAGCACGAACTGAGTTGATAGAGGAATTAAACAAGGGTGTTTCTTTGGAGAAGCTACGAGCGAAACTGACAAAGACACCTGAGGCAACTGATAGTAATGCTCCTGCATCTGAAAGCACTGTGACTACTAAAGTCCCAGAGGAACTTGTACAAGTCCAGGCTTACATAAGGTGGGAGAAAGCAGGCAAGCCAAATTATGCCCCAGAGAAGCAATTGGTCGAGTTTGAGGAAGCAAGGAAGGAACTGCAGTCTGAGTTGGATAAGGGGACCTCAGTTGAGCAGTTGAGGAACAAAATTTTGAAAGGGAACATTGAGACAAAAGTTTCCAAGCAGCTGAAGGACAAAAAATACTTTTCTGTGGAAAGAATTCAGCGGAAAAAACGAGATATTGTGCAACTACTTAAAAAACACAAGCCTACTGTTATGGAAGCGCAAGTAGAGACTCCTAAACAACCCACTGTTCTGGATCTCTTCACAAAGTCATTACAGGAGCAGGATAACTGTGAGGTTCTAAGCAGAAAGCTTTTCAAGTTCGGTGACAAGGAGATACTGGAAAAACCAAAGTTCACTTGGCAACAAACTATATGGAGCCACTTATACTTCACTGGGCGTTGTCAAAAGAGAATGGAGAGTGGCAGACAATGTGGGCCTGTACTGATGGGGTGCTTTGTAACTGTGAAGGCACCTCCCTCAAGCATATTGCCATCTGGTTCATCATTGCTAGACAAGGCATGTGAAACTTCATTCAGTGAATATGAATTGAATGGTCTGCATTGTCAGGATACTGGTGATGCTGGTAAAGGCACTGCTAAGGCCTTGCTTGAAAGAATAGCAGATCTAGAGGAAGATGCCCAACGATCTCTTATGCACAGATTCAATATTGCAGCAGATCTAGTTGACCAAGCAAGAGATAATGGATTATTGGGTATTATTGGAATTTTTGTTTGGATTAGGTTCATGGCTACAAGGCAACTAATATGGAACAAGAACTACAATGTGAAGCCACGTGAGATAAGCAAAGCACAAGATAGGTTTACAGATGATCTTGAGAATATGTACAGAACTTACCCACAATATCAGGAGATCTTAAGAATGATAATGTCTGCTGTTGGTCGGGGAGGTGAAGGTGATGTTGGTCAACGCATTCGTGATGAGATATTAGTAATCCAGAGAAATAATGACTGCAAAGGTGGAATGATGGAGGAGTGGCACCAGAAACTGCACAACAATACAAGCCCAGATGATGTAGTGATCTGCCAGGCCCTACTTGATTATATCAAGAGTGATTTTGATATTGGTGTTTACTGGGACACCTTGAAAAAAGATGGTATAACAAAAGAGCGTCTATTGAGCTATGATCGACCGATTCATTCAGAGCCAAATTTCAGGAGTGAACAGAAAGATGGCTTACTCCGTGACTTGGGCAATTATATGAGAAGCCTCAAGATGGAGGGTACCCTGATACAATCACTGCGAATGGCAGTGCATTCTGGTGCTGATCTTGAATCTGCTATAGCAACTTGCATGGGATACAAATCAGAGGGTGAAGGTTTCATGGTTGGTGTTCAGATTAATCCAGTGAAGGGTTTGCCATCTGGATTTCCTAAATTGCTTGAATTTGTACTTGACCATGTTGAGGATAAATCAGCAGAACCACTTCTTGAGGGGTTATTGGAGGCTCGAGCTGAACTACACCCTTTGCTCCTTGGCTCTCCTGAACGCATGAAGGATCTTATCTTTTTAGACATTGCTCTTGATTCTACTTTCAGGACAGCAGTTGAAAGATCATATGAGGAGCTCAATAATGTAGAACCAGAGAAAATTATGTACTTCATCAGTCTTGTCCTTGAAAATCTTGCTTTATCCACCGACGACAATGAAGATATCCTATATTGCTTAAAGGGATGGAATCAAGCCTTGGAAATGGCTAAACAGAAAAACAACCAATGGGCTCTCTATGCTAAAGCATTTCTGGACAGAACCAGACTTGCCCTTGCAAGCAAGGGAGAACAATACTATAATTTGATGCAGCCCTCAGCTGAATATCTTGGCTCGTTACTTAACATTGACCAATGGGCAGTTAATATCTTTACAGAAGAAATTATTCGTGGTGGATCAGCTGCTACCCTGTCTGCTCTTCTGAATCGGATTGATCCTGTTCTTAGGAATGTTGCACAGCTTGGAAGTTGGCAGGTTATAAGCCCAGTTGAAGTATCAGGTTACATTGTAGTGGTTGATGAATTGCTTGCTGTTCAAAACAAATCCTATGATAAACCAACTATCCTTGTGGCAAAGAGTGTCAAGGGAGAGGAAGAAATACCAGATGGAGTTGTTGGTGTTATTACACCTGATATGCCAGATGTTCTCTCCCATGTATCAGTCCGAGCAAGGAATTGCAAGGTTTTATTTGCAACATGCTTTGATCCTAACACCTTGTCTGAACTCCAAGGACATGATGGGAAAGTGTTTTCCTTCAAACCTACTTCTGCAGATATCACCTATAGGGAGATTCCAGAGAGTGAACTGCAATCAGGTTCTCTAAATGCAGAAGCTGGCCAGGCAGTGCCATCTGTGTCATTAGTCAAGAAGAAGTTTCTTGGAAAATATGCAATATCAGCAGAAGAATTCTCTGAGGAAATGGTTGGGGCCAAGTCTCGCAACGTAGCATACCTCAAAGGAAAAGTACCCTCATGGGTTGGTGTCCCTACATCAGTTGCGATTCCATTTGGGACCTTTGAGAAGGTTTTGTCTGATGAAATCAATAAGGAAGTCGCGCAAACCATACAAATGCTGAAGGGAAAACTTGCTCAAGATGATTTTAGTGCTCTAGGCGAAATACGGAAAACTGTTCTCAATTTAACTGCTCCTACTCAACTGATCAAGGAACTGAAGGAGAAGATGCTAGGCTCTGGAATGCCCTGGCCTGGAGATGAAGGTGACCAACGTTGGGAGCAAGCATGGATGGCAATTAAAAAGGTTTGGGCGTCAAAATGGAATGAAAGAGCATATTTTAGCACTCGTAAGGTGAAGCTTGATCATGACTACCTTTCCATGGCTGTACTTGTACAAGAAATTGTCAATGCAGACTATGCCTTTGTCATTCATACTACTAACCCATCATCGGGAGATTCGTCTGAGATATATGCTGAAGTGGTGAAAGGGCTTGGAGAAACACTTGTAGGAGCCTATCCTGGTCGCGCCATGAGCTTTGTATGTAAGAAAAACGACCTTGACTCTCCCAAGGTACTGGGTTTCCCAAGCAAGCCAATTGGTCTCTTCATAAAGAGATCAATCATCTTTCGTTCAGATTCCAACGGTGAGGATTTAGAAGGGTATGCTGGAGCAGGACTGTATGATAGTTCGCTCAAGAATTTATTTGGCATTACAACTAAGCTGACTGCTTGTTTCAGTGTCCCTATGGATGAGGAAGATGAAGTCATACTCGACTACACCACCGACCCCCTCATTACAGATCAGGGATTCCAAAAATCTATCCTCTCGAGCATTGCACGGGCTGGTCATGCCATTGAGGAGCTTTATGGGTCCCCACAGGATGTTGAGGGTGCAGTGAAGGAAGGGAAGCTATACGTAGAGATCAAGGAGGCTTACAGGAAGCTCCAGAAACGACACCATATCCTGATATTGCTGGCTACAAGGGTCATGCATGACTACACCCTACTGCTGAATGAGGCATACAAGGTATTGATGAGGAATTAA
CDS Sequence
  • >LOC_Os06g30310.1
    ATGGCGCTAAGGAAGGAGAGATATCCATCCGGATCCCGGAAGCCGAATCCATCCATCCATCCATCCCATACTGCCCTTACGATCGAGCTGTTTGATATTCGTGCAGATGAGCGGATTCTCCGCGGCAGCTGCTGCGGCCGAGCGCTTGTCGGAAGGTTCACCCTGGATGCCAACTCCGAGCTTAAGGTGACATTGAACCCAGCACCGCAGGGTTCGGTGGTGGAGATCAATCTAGAGGCAACTAACACCAGCGGCTCCCTGATACTGCATTGGGGCGCCCTTCGCCCGGATAGAGGAGAATGGCTCCTACCATCCCGGAAACCAGATGGCACGACAGTGTACAAGAACAGGGCTCTTAGGACGCCTTTTATAAAGTCAGGTGATAACTCCACGCTGAAAATTGAGATAGATGATCCTGCAGTGCAAGCCATTGAGTTCCTCATATTTGATGAGGCACGGAATAATTGGTCAGTTCTTTTTGGGTTACCCATCCTACCCTGTTTCTTGGAATGCAGAAGATATCAAATAACAACACTCTACTTTGGACAGGAGGAGTATGAAGCAGCACGAACTGAGTTGATAGAGGAATTAAACAAGGGTGTTTCTTTGGAGAAGCTACGAGCGAAACTGACAAAGACACCTGAGGCAACTGATAGTAATGCTCCTGCATCTGAAAGCACTGTGACTACTAAAGTCCCAGAGGAACTTGTACAAGTCCAGGCTTACATAAGGTGGGAGAAAGCAGGCAAGCCAAATTATGCCCCAGAGAAGCAATTGGTCGAGTTTGAGGAAGCAAGGAAGGAACTGCAGTCTGAGTTGGATAAGGGGACCTCAGTTGAGCAGTTGAGGAACAAAATTTTGAAAGGGAACATTGAGACAAAAGTTTCCAAGCAGCTGAAGGACAAAAAATACTTTTCTGTGGAAAGAATTCAGCGGAAAAAACGAGATATTGTGCAACTACTTAAAAAACACAAGCCTACTGTTATGGAAGCGCAAGTAGAGACTCCTAAACAACCCACTGTTCTGGATCTCTTCACAAAGTCATTACAGGAGCAGGATAACTGTGAGGTTCTAAGCAGAAAGCTTTTCAAGTTCGGTGACAAGGAGATACTGGAAAAACCAAAGTTCACTTGGCAACAAACTATATGGAGCCACTTATACTTCACTGGGCGTTGTCAAAAGAGAATGGAGAGTGGCAGACAATGTGGGCCTGTACTGATGGGGTGCTTTGTAACTGTGAAGGCACCTCCCTCAAGCATATTGCCATCTGGTTCATCATTGCTAGACAAGGCATGTGAAACTTCATTCAGTGAATATGAATTGAATGGTCTGCATTGTCAGGATACTGGTGATGCTGGTAAAGGCACTGCTAAGGCCTTGCTTGAAAGAATAGCAGATCTAGAGGAAGATGCCCAACGATCTCTTATGCACAGATTCAATATTGCAGCAGATCTAGTTGACCAAGCAAGAGATAATGGATTATTGGGTATTATTGGAATTTTTGTTTGGATTAGGTTCATGGCTACAAGGCAACTAATATGGAACAAGAACTACAATGTGAAGCCACGTGAGATAAGCAAAGCACAAGATAGGTTTACAGATGATCTTGAGAATATGTACAGAACTTACCCACAATATCAGGAGATCTTAAGAATGATAATGTCTGCTGTTGGTCGGGGAGGTGAAGGTGATGTTGGTCAACGCATTCGTGATGAGATATTAGTAATCCAGAGAAATAATGACTGCAAAGGTGGAATGATGGAGGAGTGGCACCAGAAACTGCACAACAATACAAGCCCAGATGATGTAGTGATCTGCCAGGCCCTACTTGATTATATCAAGAGTGATTTTGATATTGGTGTTTACTGGGACACCTTGAAAAAAGATGGTATAACAAAAGAGCGTCTATTGAGCTATGATCGACCGATTCATTCAGAGCCAAATTTCAGGAGTGAACAGAAAGATGGCTTACTCCGTGACTTGGGCAATTATATGAGAAGCCTCAAGATGGAGGGTACCCTGATACAATCACTGCGAATGGCAGTGCATTCTGGTGCTGATCTTGAATCTGCTATAGCAACTTGCATGGGATACAAATCAGAGGGTGAAGGTTTCATGGTTGGTGTTCAGATTAATCCAGTGAAGGGTTTGCCATCTGGATTTCCTAAATTGCTTGAATTTGTACTTGACCATGTTGAGGATAAATCAGCAGAACCACTTCTTGAGGGGTTATTGGAGGCTCGAGCTGAACTACACCCTTTGCTCCTTGGCTCTCCTGAACGCATGAAGGATCTTATCTTTTTAGACATTGCTCTTGATTCTACTTTCAGGACAGCAGTTGAAAGATCATATGAGGAGCTCAATAATGTAGAACCAGAGAAAATTATGTACTTCATCAGTCTTGTCCTTGAAAATCTTGCTTTATCCACCGACGACAATGAAGATATCCTATATTGCTTAAAGGGATGGAATCAAGCCTTGGAAATGGCTAAACAGAAAAACAACCAATGGGCTCTCTATGCTAAAGCATTTCTGGACAGAACCAGACTTGCCCTTGCAAGCAAGGGAGAACAATACTATAATTTGATGCAGCCCTCAGCTGAATATCTTGGCTCGTTACTTAACATTGACCAATGGGCAGTTAATATCTTTACAGAAGAAATTATTCGTGGTGGATCAGCTGCTACCCTGTCTGCTCTTCTGAATCGGATTGATCCTGTTCTTAGGAATGTTGCACAGCTTGGAAGTTGGCAGGTTATAAGCCCAGTTGAAGTATCAGGTTACATTGTAGTGGTTGATGAATTGCTTGCTGTTCAAAACAAATCCTATGATAAACCAACTATCCTTGTGGCAAAGAGTGTCAAGGGAGAGGAAGAAATACCAGATGGAGTTGTTGGTGTTATTACACCTGATATGCCAGATGTTCTCTCCCATGTATCAGTCCGAGCAAGGAATTGCAAGGTTTTATTTGCAACATGCTTTGATCCTAACACCTTGTCTGAACTCCAAGGACATGATGGGAAAGTGTTTTCCTTCAAACCTACTTCTGCAGATATCACCTATAGGGAGATTCCAGAGAGTGAACTGCAATCAGGTTCTCTAAATGCAGAAGCTGGCCAGGCAGTGCCATCTGTGTCATTAGTCAAGAAGAAGTTTCTTGGAAAATATGCAATATCAGCAGAAGAATTCTCTGAGGAAATGGTTGGGGCCAAGTCTCGCAACGTAGCATACCTCAAAGGAAAAGTACCCTCATGGGTTGGTGTCCCTACATCAGTTGCGATTCCATTTGGGACCTTTGAGAAGGTTTTGTCTGATGAAATCAATAAGGAAGTCGCGCAAACCATACAAATGCTGAAGGGAAAACTTGCTCAAGATGATTTTAGTGCTCTAGGCGAAATACGGAAAACTGTTCTCAATTTAACTGCTCCTACTCAACTGATCAAGGAACTGAAGGAGAAGATGCTAGGCTCTGGAATGCCCTGGCCTGGAGATGAAGGTGACCAACGTTGGGAGCAAGCATGGATGGCAATTAAAAAGGTTTGGGCGTCAAAATGGAATGAAAGAGCATATTTTAGCACTCGTAAGGTGAAGCTTGATCATGACTACCTTTCCATGGCTGTACTTGTACAAGAAATTGTCAATGCAGACTATGCCTTTGTCATTCATACTACTAACCCATCATCGGGAGATTCGTCTGAGATATATGCTGAAGTGGTGAAAGGGCTTGGAGAAACACTTGTAGGAGCCTATCCTGGTCGCGCCATGAGCTTTGTATGTAAGAAAAACGACCTTGACTCTCCCAAGGTACTGGGTTTCCCAAGCAAGCCAATTGGTCTCTTCATAAAGAGATCAATCATCTTTCGTTCAGATTCCAACGGTGAGGATTTAGAAGGGTATGCTGGAGCAGGACTGTATGATAGTTCGCTCAAGAATTTATTTGGCATTACAACTAAGCTGACTGCTTGTTTCAGTGTCCCTATGGATGAGGAAGATGAAGTCATACTCGACTACACCACCGACCCCCTCATTACAGATCAGGGATTCCAAAAATCTATCCTCTCGAGCATTGCACGGGCTGGTCATGCCATTGAGGAGCTTTATGGGTCCCCACAGGATGTTGAGGGTGCAGTGAAGGAAGGGAAGCTATACGTAGAGATCAAGGAGGCTTACAGGAAGCTCCAGAAACGACACCATATCCTGATATTGCTGGCTACAAGGGTCATGCATGACTACACCCTACTGCTGAATGAGGCATACAAGGTATTGATGAGGAATTAA
Protein Sequence
  • >LOC_Os06g30310.1
    MALRKERYPSGSRKPNPSIHPSHTALTIELFDIRADERILRGSCCGRALVGRFTLDANSELKVTLNPAPQGSVVEINLEATNTSGSLILHWGALRPDRGEWLLPSRKPDGTTVYKNRALRTPFIKSGDNSTLKIEIDDPAVQAIEFLIFDEARNNWSVLFGLPILPCFLECRRYQITTLYFGQEEYEAARTELIEELNKGVSLEKLRAKLTKTPEATDSNAPASESTVTTKVPEELVQVQAYIRWEKAGKPNYAPEKQLVEFEEARKELQSELDKGTSVEQLRNKILKGNIETKVSKQLKDKKYFSVERIQRKKRDIVQLLKKHKPTVMEAQVETPKQPTVLDLFTKSLQEQDNCEVLSRKLFKFGDKEILEKPKFTWQQTIWSHLYFTGRCQKRMESGRQCGPVLMGCFVTVKAPPSSILPSGSSLLDKACETSFSEYELNGLHCQDTGDAGKGTAKALLERIADLEEDAQRSLMHRFNIAADLVDQARDNGLLGIIGIFVWIRFMATRQLIWNKNYNVKPREISKAQDRFTDDLENMYRTYPQYQEILRMIMSAVGRGGEGDVGQRIRDEILVIQRNNDCKGGMMEEWHQKLHNNTSPDDVVICQALLDYIKSDFDIGVYWDTLKKDGITKERLLSYDRPIHSEPNFRSEQKDGLLRDLGNYMRSLKMEGTLIQSLRMAVHSGADLESAIATCMGYKSEGEGFMVGVQINPVKGLPSGFPKLLEFVLDHVEDKSAEPLLEGLLEARAELHPLLLGSPERMKDLIFLDIALDSTFRTAVERSYEELNNVEPEKIMYFISLVLENLALSTDDNEDILYCLKGWNQALEMAKQKNNQWALYAKAFLDRTRLALASKGEQYYNLMQPSAEYLGSLLNIDQWAVNIFTEEIIRGGSAATLSALLNRIDPVLRNVAQLGSWQVISPVEVSGYIVVVDELLAVQNKSYDKPTILVAKSVKGEEEIPDGVVGVITPDMPDVLSHVSVRARNCKVLFATCFDPNTLSELQGHDGKVFSFKPTSADITYREIPESELQSGSLNAEAGQAVPSVSLVKKKFLGKYAISAEEFSEEMVGAKSRNVAYLKGKVPSWVGVPTSVAIPFGTFEKVLSDEINKEVAQTIQMLKGKLAQDDFSALGEIRKTVLNLTAPTQLIKELKEKMLGSGMPWPGDEGDQRWEQAWMAIKKVWASKWNERAYFSTRKVKLDHDYLSMAVLVQEIVNADYAFVIHTTNPSSGDSSEIYAEVVKGLGETLVGAYPGRAMSFVCKKNDLDSPKVLGFPSKPIGLFIKRSIIFRSDSNGEDLEGYAGAGLYDSSLKNLFGITTKLTACFSVPMDEEDEVILDYTTDPLITDQGFQKSILSSIARAGHAIEELYGSPQDVEGAVKEGKLYVEIKEAYRKLQKRHHILILLATRVMHDYTLLLNEAYKVLMRN*