Information report for OsOTS1
Gene Details
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Functional Descriptions
- We demonstrate that OsOTS1 confers salt tolerance in rice by increasing root biomass.
- High salinity triggers OsOTS1 degradation indicating that increased SUMO conjugation in rice plants during salt stress is in part achieved by down-regulation of OTS1/2 activity.
- In this report, we reveal the Ubiquitin-like Protease (ULP) class of SUMO protease gene family in rice and demonstrate a critical role for OsOTS1 SUMO protease in salt stress.
- Transgenic rice plants overexpressing OsOTS1 have increased salt tolerance and a concomitant reduction in the levels of SUMOylated proteins.
- OsOTS1 is nuclear localised indicating a direct requirement of OsOTS1 dependent deSUMOylation activity in rice nuclei for salt tolerance.
- Knockdown of OsOTS1 gene expression affects root growth by primarily reducing cell size rather than cell division.
- We recently identified the SUMO protease gene family in rice and demonstrated a role for OsOTS1 SUMO proteases in salt stress.
- We reveal that OsOTS1 SUMO protease directly targets the ABA and drought responsive transcription factor OsbZIP23 for de-SUMOylation affecting its stability.
- In this study, we reveal the role of the SUMO protease, OsOTS1 in mediating tolerance to drought in rice.
- OsOTS1 depleted transgenic plants accumulate more ABA and exhibit more productive agronomic traits during drought whilst OsOTS1 overexpressing lines are drought sensitive but ABA insensitive.
- Drought and ABA treatment stimulates the degradation of OsOTS1 protein indicating that SUMO conjugation is an important response to drought stress in rice achieved through down-regulation of OTS1/2 activity.
- OsOTS-RNAi lines show increased abundance of OsbZIP23 and increased drought responsive gene expression while OsOTS1 overexpressing lines show reduced levels of OsbZIP23 leading to suppressed drought responsive gene expression.
Functional Keywords
- root , salinity , salt , tolerance , salt-tolerance , salt-stress , stress , biomass , growth , cell-division , transcription-factor , drought , ABA , drought-stress
Literature and News
- SUMO Is a Critical Regulator of Salt Stress Responses in Rice . DOI: 10.1104/pp.15.01530 ; PMID: 26869703
- Rice OVERLY TOLERANT TO SALT 1 (OTS1) SUMO protease is a positive regulator of seed germination and root development . DOI: 10.1080/15592324.2016.1173301 ; PMID: 27119209
- Rice SUMO protease Overly Tolerant to Salt 1 targets the transcription factor, OsbZIP23 to promote drought tolerance in rice . DOI: 10.1111/tpj.13739 ; PMID: 29024118
Gene Resources
- UniProt: Q67VX6
- EMBL: AK064330, AP014962
- AlphaFoldDB: Q67VX6
- EnsemblPlants: Os06t0487900-01
- Gramene: Os06t0487900-01
- KEGG: osa:4341072
- Orthologous matrix: HADAHWS
- InterPro: IPR003653, IPR038765
- PANTHER: PTHR46915, PTHR46915:SF2
- SUPFAM: SSF54001
- PROSITE: PS50600
- Gene3D: 1.10.418.20, 3.30.310.130
- OrthoDB: Q67VX6
- SWISS-MODEL: Q67VX6
- eggNOG: KOG0779
Sequences
cDNA Sequence
- >LOC_Os06g29310.1
AGCACTGCACGCGTTCCTGCTCCTTGTGTTCCTCTCTCCTCTCCTCTCCTCCGCGTAGGGGGTAGAGTAGGGGAATCCGCGCCGCCTCCTGCTCCTGGCCGCCGTGAATGCTATGGCCGGCGGCGAGGACGGGGCCGACGGCGTCGGAGATGACACCGAGCTTCGCAGGTTCTCAGACGTGGAGCTGCTGGAGAAGTCGCGGCGCATTCAGGGGGAGCTCAGTGGGGGTATCGAGCGAAGGCTCAAGGACCGCGGGGCCAAGTTTCGCCGCTTGCTAGACGCCATCGTCCGCGAGATTGATCGCCGTAAGGCAGCAACGGATGATGACAGATGCGCAAGGGTAGTTCAATCGGGATGTGCAGAATCATCAGTTAAACAACAAGCAGTAACCATATCAGATTTTCGGTCCTCCTTTGGGATAGATGAAGAGGCAGGCGTTGATGTATCTCATTTGGAAACAAGTGCATGCATTGGCGACCCAAAAACTTCAACAGATAATGAGGGGATCTTGTGTGAAGAGGAAGATTCCTGTAAGTGTAAACCCTCTACGTCTCAAAAGGCATCATATGTTGATAGGTCAACTTATATGGAAAATATAGATGCTGCTGATGATGGCAAGGATAATGGCTACAGTAGGATATGCAAAGATGCCCACACTTCTAGGAAAAGGAATGGAGAATTTTCACCTACCTTTTCAATGCGATTGCGATCAAGAAAGGTGGTTGAAGAGGTGGTTCTACTCGATGGAGATACTTGCATTTCAGACTCTGCAGAAAAAACATCCTCTGCATGTGATGCAATGAAGATACACTATCCTTCATGGGACACCCCTAATTCTATTGAGCTTTCTTATTCTGACATGAAGTGTCTTGAGCCTGAATCATTGTTGTCCTCACCTATACTGAACTTCTACATTATGTACCTAATGGGACAAATGCCATCGACAAGTAGGCTTGGAGGCAAGTATCATATTTTCAATACATACTTCTTCAGCAAACTTGAAGCCTTGACATCAAAGGTGGACAATGATGCTTATTTCTTGAATCTGAGAAGATGGTGGAAAGGTGTAGATATATTTAAAAAGGCATACATAATAATTCCTGTGCATGCAGACGCACACTGGAGCTTGGTCATTATATGTATGCCTGCAAAGGAAGACCAATCAGGGCCCACCATATTTCATTTGGATTCACTGAAGTTTCATAGCAGCAGATTTATTTTCAGCACCGTTGAGAGATTCCTTAAAGAAGAATGGAACTACCTGAATAAAACTGGTTCTTTAGAAGATTGTCATCTACATGAATCAGTGTGGAAGAACCTTCCTCGGAAAATTAAGAAGAAAGCAGTTACAGTTCCACAGCAGGACAATGAATACGATTGTGGTGTCTTTGTTCTGTACTATATGCGACGCTTTATCGAGGAAGCACCTGAAAGGCTTAACAACAAGGACTCATCTAATATGTTTGGCGAAGGGTGGTTCCAACGTGAAGAGGCTTCTGCACTGCGAAAGGAAATGCAAGCTCTGCTTCTTCGGTTGTTTGAGGAAGCTAAAGACAACAACCACATGAGAGATCCAACAACACCAGTGTCAGCAACAGCGGAGCACCCTGTTGAAGTCTTGTCAACCGAGCCAGCAGTACCAGACCACCCTCGGAATGCGGTTGGCGTCGCCACCTCATAGTTTGCAGGCGGCTGCCATGGGAGCTGGCCTTGGAATGTGGAAGGTTGGTGTGCATCCGGAAAACATAGCTCTCAATTGTCTTCTCCGGTTATGCCACAAGAGAAGGAAACTTGGAAGGTACTGTACTGGGTACTATGGTAGGTAGGTCTGCAAAGATGCGATACTGCCGTGTATAGTTTAGGTAGAAAGCCGGGTTATCCGCTGGGCTCTTGGGGATAATTTTGGTTCTTGGTTAGTTCTTAGGCTGGGCTACAAGAGAATGGTCGAATTTGACAAACTGCTCCTACAAGCTCTGAGCTGCCAAGTTGACAGCTGCAGGATCTGCTTGTAAACCCATGAGGAAGGGGAGAATCATCAGAATAGCTCTCACCTGCCTGCCCAAATTTCTGCTTGTAAAGCATTCTGAATTGAAGTTCTGATGGGTATAGCCGTCGGTGGAGTGGAGGCACAACTTTTGGAGTTGGATACTGTCATCGCTCTTAAAACCATGCTTTTGGGGACACTTGTACATTTGGTGCTAGTAAAAGTCAGGCGGCATCACGGAGTTCACA - >LOC_Os06g29310.2
AGCACTGCACGCGTTCCTGCTCCTTGTGTTCCTCTCTCCTCTCCTCTCCTCCGCGTAGGGGGTAGAGTAGGGGAATCCGCGCCGCCTCCTGCTCCTGGCCGCCGTGAATGCTATGGCCGGCGGCGAGGACGGGGCCGACGGCGTCGGAGATGACACCGAGCTTCGCAGGTTCTCAGACGTGGAGCTGCTGGAGAAGTCGCGGCGCATTCAGGGGGAGCTCAGTGGGGGTATCGAGCGAAGGCTCAAGGACCGCGGGGCCAAGTTTCGCCGCTTGCTAGACGCCATCGTCCGCGAGATTGATCGCCGTAAGGCAGCAACGGATGATGACAGATGCGCAAGGGTAGTTCAATCGGGATGTGCAGAATCATCAGTTAAACAACAAGCAGTAACCATATCAGATTTTCGGTCCTCCTTTGGGATAGATGAAGAGGCAGGCGTTGATGTATCTCATTTGGAAACAAGTGCATGCATTGGCGACCCAAAAACTTCAACAGATAATGAGGGGATCTTGTGTGAAGAGGAAGATTCCTGTAAGTGTAAACCCTCTACGTCTCAAAAGGCATCATATGTTGATAGGTCAACTTATATGGAAAATATAGATGCTGCTGATGATGGCAAGGATAATGGCTACAGTAGGATATGCAAAGATGCCCACACTTCTAGGAAAAGGAATGGAGAATTTTCACCTACCTTTTCAATGCGATTGCGATCAAGAAAGGTGGTTGAAGAGGTGGTTCTACTCGATGGAGATACTTGCATTTCAGACTCTGCAGAAAAAACATCCTCTGCATGTGATGCAATGAAGATACACTATCCTTCATGGGACACCCCTAATTCTATTGAGCTTTCTTATTCTGACATGAAGTGTCTTGAGCCTGAATCATTGTTGTCCTCACCTATACTGAACTTCTACATTATGTACCTAATGGGACAAATGCCATCGACAAGTAGGCTTGGAGGCAAGTATCATATTTTCAATACATACTTCTTCAGCAAACTTGAAGCCTTGACATCAAAGGTGGACAATGATGCTTATTTCTTGAATCTGAGAAGATGGTGGAAAGGTGTAGATATATTTAAAAAGGCATACATAATAATTCCTGTGCATGCAGACGCACACTGGAGCTTGGTCATTATATGTATGCCTGCAAAGGAAGACCAATCAGGGCCCACCATATTTCATTTGGATTCACTGAAGTTTCATAGCAGCAGATTTATTTTCAGCACCGTTGAGAGATTCCTTAAAGAAGAATGGAACTACCTGAATAAAACTGGTTCTTTAGAAGATTGTCATCTACATGAATCAGTGTGGAAGAACCTTCCTCGGAAAATTAAGAAGAAAGCAGTTACAGTTCCACAGCAGGACAATGAATACGATTGTGGTGTCTTTGTTCTGTACTATATGCGACGCTTTATCGAGGAAGCACCTGAAAGGCTTAACAACAAGGACTCATCTAATATGTTTGGCGAAGGGTGGTTCCAACGTGAAGAGGCTTCTGCACTGCGAAAGGAAATGCAAGCTCTGCTTCTTCGGTTGTTTGAGGAAGCTAAAGACAACAACCACATGAGAGATCCAACAACACCAGTGTCAGCAACAGCGGAGCACCCTGTTGAAGTCTTGTCAACCGAGCCAGCAGTACCAGACCACCCTCGGAATGCGGTTGGCGTCGCCACCTCATAGTTTGCAGGCGGCTGCCATGGGAGCTGGCCTTGGAATGTGGAAGGTTGGTGTGCATCCGGAAAACATAGCTCTCAATTGTCTTCTCCGGTTATGCCACAAGAGAAGGAAACTTGGAAGGTACTGTACTGGGTACTATGGTAGGTAGGTCTGCAAAGATGCGATACTGCCGTGTATAGTTTAGGTAGAAAGCCGGGTTATCCGCTGGGCTCTTGGGGATAATTTTGGTTCTTGGTTAGTTCTTAGGCTGGGCTACAAGAGAATGGTCGAATTTGACAAACTGCTCCTACAAGCTCTGAGCTGCCAAGTTGACAGCTGCAGGATCTGCTTGTAAACCCATGAGGAAGGGGAGAATCATCAGAATAGCTCTCACCTGCCTGCCCAAATTTCTGCTTGTAAAGCATTCTGAATTGAAGTTCTGATGGGTATAGCCGTCGGTGGAGTGGAGGCACAACTTTTGGAGTTGGATACTGTCATCGCTCTTAAAACCATGCTTTTGGGGACACTTGTACATTTGGTGCTAGTAAAAGTCAGGCGGCATCACGGAGTTCACA
CDS Sequence
- >LOC_Os06g29310.1
ATGGCCGGCGGCGAGGACGGGGCCGACGGCGTCGGAGATGACACCGAGCTTCGCAGGTTCTCAGACGTGGAGCTGCTGGAGAAGTCGCGGCGCATTCAGGGGGAGCTCAGTGGGGGTATCGAGCGAAGGCTCAAGGACCGCGGGGCCAAGTTTCGCCGCTTGCTAGACGCCATCGTCCGCGAGATTGATCGCCGTAAGGCAGCAACGGATGATGACAGATGCGCAAGGGTAGTTCAATCGGGATGTGCAGAATCATCAGTTAAACAACAAGCAGTAACCATATCAGATTTTCGGTCCTCCTTTGGGATAGATGAAGAGGCAGGCGTTGATGTATCTCATTTGGAAACAAGTGCATGCATTGGCGACCCAAAAACTTCAACAGATAATGAGGGGATCTTGTGTGAAGAGGAAGATTCCTGTAAGTGTAAACCCTCTACGTCTCAAAAGGCATCATATGTTGATAGGTCAACTTATATGGAAAATATAGATGCTGCTGATGATGGCAAGGATAATGGCTACAGTAGGATATGCAAAGATGCCCACACTTCTAGGAAAAGGAATGGAGAATTTTCACCTACCTTTTCAATGCGATTGCGATCAAGAAAGGTGGTTGAAGAGGTGGTTCTACTCGATGGAGATACTTGCATTTCAGACTCTGCAGAAAAAACATCCTCTGCATGTGATGCAATGAAGATACACTATCCTTCATGGGACACCCCTAATTCTATTGAGCTTTCTTATTCTGACATGAAGTGTCTTGAGCCTGAATCATTGTTGTCCTCACCTATACTGAACTTCTACATTATGTACCTAATGGGACAAATGCCATCGACAAGTAGGCTTGGAGGCAAGTATCATATTTTCAATACATACTTCTTCAGCAAACTTGAAGCCTTGACATCAAAGGTGGACAATGATGCTTATTTCTTGAATCTGAGAAGATGGTGGAAAGGTGTAGATATATTTAAAAAGGCATACATAATAATTCCTGTGCATGCAGACGCACACTGGAGCTTGGTCATTATATGTATGCCTGCAAAGGAAGACCAATCAGGGCCCACCATATTTCATTTGGATTCACTGAAGTTTCATAGCAGCAGATTTATTTTCAGCACCGTTGAGAGATTCCTTAAAGAAGAATGGAACTACCTGAATAAAACTGGTTCTTTAGAAGATTGTCATCTACATGAATCAGTGTGGAAGAACCTTCCTCGGAAAATTAAGAAGAAAGCAGTTACAGTTCCACAGCAGGACAATGAATACGATTGTGGTGTCTTTGTTCTGTACTATATGCGACGCTTTATCGAGGAAGCACCTGAAAGGCTTAACAACAAGGACTCATCTAATATGTTTGGCGAAGGGTGGTTCCAACGTGAAGAGGCTTCTGCACTGCGAAAGGAAATGCAAGCTCTGCTTCTTCGGTTGTTTGAGGAAGCTAAAGACAACAACCACATGAGAGATCCAACAACACCAGTGTCAGCAACAGCGGAGCACCCTGTTGAAGTCTTGTCAACCGAGCCAGCAGTACCAGACCACCCTCGGAATGCGGTTGGCGTCGCCACCTCATAG - >LOC_Os06g29310.2
ATGGCCGGCGGCGAGGACGGGGCCGACGGCGTCGGAGATGACACCGAGCTTCGCAGGTTCTCAGACGTGGAGCTGCTGGAGAAGTCGCGGCGCATTCAGGGGGAGCTCAGTGGGGGTATCGAGCGAAGGCTCAAGGACCGCGGGGCCAAGTTTCGCCGCTTGCTAGACGCCATCGTCCGCGAGATTGATCGCCGTAAGGCAGCAACGGATGATGACAGATGCGCAAGGGTAGTTCAATCGGGATGTGCAGAATCATCAGTTAAACAACAAGCAGTAACCATATCAGATTTTCGGTCCTCCTTTGGGATAGATGAAGAGGCAGGCGTTGATGTATCTCATTTGGAAACAAGTGCATGCATTGGCGACCCAAAAACTTCAACAGATAATGAGGGGATCTTGTGTGAAGAGGAAGATTCCTGTAAGTGTAAACCCTCTACGTCTCAAAAGGCATCATATGTTGATAGGTCAACTTATATGGAAAATATAGATGCTGCTGATGATGGCAAGGATAATGGCTACAGTAGGATATGCAAAGATGCCCACACTTCTAGGAAAAGGAATGGAGAATTTTCACCTACCTTTTCAATGCGATTGCGATCAAGAAAGGTGGTTGAAGAGGTGGTTCTACTCGATGGAGATACTTGCATTTCAGACTCTGCAGAAAAAACATCCTCTGCATGTGATGCAATGAAGATACACTATCCTTCATGGGACACCCCTAATTCTATTGAGCTTTCTTATTCTGACATGAAGTGTCTTGAGCCTGAATCATTGTTGTCCTCACCTATACTGAACTTCTACATTATGTACCTAATGGGACAAATGCCATCGACAAGTAGGCTTGGAGGCAAGTATCATATTTTCAATACATACTTCTTCAGCAAACTTGAAGCCTTGACATCAAAGGTGGACAATGATGCTTATTTCTTGAATCTGAGAAGATGGTGGAAAGGTGTAGATATATTTAAAAAGGCATACATAATAATTCCTGTGCATGCAGACGCACACTGGAGCTTGGTCATTATATGTATGCCTGCAAAGGAAGACCAATCAGGGCCCACCATATTTCATTTGGATTCACTGAAGTTTCATAGCAGCAGATTTATTTTCAGCACCGTTGAGAGATTCCTTAAAGAAGAATGGAACTACCTGAATAAAACTGGTTCTTTAGAAGATTGTCATCTACATGAATCAGTGTGGAAGAACCTTCCTCGGAAAATTAAGAAGAAAGCAGTTACAGTTCCACAGCAGGACAATGAATACGATTGTGGTGTCTTTGTTCTGTACTATATGCGACGCTTTATCGAGGAAGCACCTGAAAGGCTTAACAACAAGGACTCATCTAATATGTTTGGCGAAGGGTGGTTCCAACGTGAAGAGGCTTCTGCACTGCGAAAGGAAATGCAAGCTCTGCTTCTTCGGTTGTTTGAGGAAGCTAAAGACAACAACCACATGAGAGATCCAACAACACCAGTGTCAGCAACAGCGGAGCACCCTGTTGAAGTCTTGTCAACCGAGCCAGCAGTACCAGACCACCCTCGGAATGCGGTTGGCGTCGCCACCTCATAG
Protein Sequence
- >LOC_Os06g29310.1
MAGGEDGADGVGDDTELRRFSDVELLEKSRRIQGELSGGIERRLKDRGAKFRRLLDAIVREIDRRKAATDDDRCARVVQSGCAESSVKQQAVTISDFRSSFGIDEEAGVDVSHLETSACIGDPKTSTDNEGILCEEEDSCKCKPSTSQKASYVDRSTYMENIDAADDGKDNGYSRICKDAHTSRKRNGEFSPTFSMRLRSRKVVEEVVLLDGDTCISDSAEKTSSACDAMKIHYPSWDTPNSIELSYSDMKCLEPESLLSSPILNFYIMYLMGQMPSTSRLGGKYHIFNTYFFSKLEALTSKVDNDAYFLNLRRWWKGVDIFKKAYIIIPVHADAHWSLVIICMPAKEDQSGPTIFHLDSLKFHSSRFIFSTVERFLKEEWNYLNKTGSLEDCHLHESVWKNLPRKIKKKAVTVPQQDNEYDCGVFVLYYMRRFIEEAPERLNNKDSSNMFGEGWFQREEASALRKEMQALLLRLFEEAKDNNHMRDPTTPVSATAEHPVEVLSTEPAVPDHPRNAVGVATS* - >LOC_Os06g29310.2
MAGGEDGADGVGDDTELRRFSDVELLEKSRRIQGELSGGIERRLKDRGAKFRRLLDAIVREIDRRKAATDDDRCARVVQSGCAESSVKQQAVTISDFRSSFGIDEEAGVDVSHLETSACIGDPKTSTDNEGILCEEEDSCKCKPSTSQKASYVDRSTYMENIDAADDGKDNGYSRICKDAHTSRKRNGEFSPTFSMRLRSRKVVEEVVLLDGDTCISDSAEKTSSACDAMKIHYPSWDTPNSIELSYSDMKCLEPESLLSSPILNFYIMYLMGQMPSTSRLGGKYHIFNTYFFSKLEALTSKVDNDAYFLNLRRWWKGVDIFKKAYIIIPVHADAHWSLVIICMPAKEDQSGPTIFHLDSLKFHSSRFIFSTVERFLKEEWNYLNKTGSLEDCHLHESVWKNLPRKIKKKAVTVPQQDNEYDCGVFVLYYMRRFIEEAPERLNNKDSSNMFGEGWFQREEASALRKEMQALLLRLFEEAKDNNHMRDPTTPVSATAEHPVEVLSTEPAVPDHPRNAVGVATS*