Gene Details:

Functional Descriptions:

  • Using rice expression-microarray and northern blot analyses, we found that a large number of nucleus-encoded genes involved in chloroplast functions were highly expressed and transcripts of plastid-encoded genes, psaA, psbA and rbcL, increased in the OsGLK1-FOX calli.
  • Transmission electron microscopy showed the existence of differentiated chloroplasts with grana stacks in OsGLK1-FOX calli cells.
  • Furthermore, we found developed chloroplasts in vascular bundle and bundle sheath cells of coleoptiles and leaves from OsGLK1-FOX seedlings.
  • The OsGLK1-FOX calli exhibited high photosynthetic activity and were able to grow on sucrose-depleted media, indicating that developed chloroplasts in OsGLK1-FOX rice calli are functional and active.
  • These results strongly suggest that OsGLK1 regulates chloroplast development under the control of light and phytohormones, and that it is a key regulator of chloroplast development.
  • Ectopic overexpression of the transcription factor OsGLK1 induces chloroplast development in non-green rice cells.
  • In both lines, OsGLK1 cDNA encoding a GARP transcription factor was ectopically overexpressed.
  • Our results revealed that OsGLK1 confers favorable characters in rice endosperm and could help to refine strategies for the carotenoid and other plastid-synthesized micronutrient fortification in bioengineered plants.
  • A major resistance QTL was identified on chromosome 6 in rice variety Wuke; both overexpression and knockdown experiments confirmed that OsGLK1 is the candidate gene for association with Rice black-streaked dwarf virus disease.
  • Furthermore, OsGLK1 knockdown rice lines were investigated and the resistance ability was significantly declined without this gene compared to the wild type.
  • Taken together, both overexpression and knockdown experiments strongly suggested that OsGLK1 plays an important role for RBSDV resistance and contributes to the major QTL.
  • The study paves the way for elucidating the molecular mechanism underlying RBSDVD resistance and the molecular markers associated with OsGLK1 may be used for marker-assisted selection.

Literature:

Gene Resources:

Sequences:

cDNA Sequence
  • >LOC_Os06g24070.1
    CTCGCAACTAACAATACCTCGCACCTAGTCACCACCGCACCAACCGCGCGCGACCGGCCCCCCGTCAGTCGAAAGCTGAGCCTGAGCGAGCAACTGATGCCGCTAGCTATAGCTGCTGCCTGCAGCACGCAGCGCCCGTAACCTAACGTATCATTTACTTCCATTGCACTGCACATCCTGTGAGTTCGTTGTCTGAGTGAGAGCTGGACGTGCTGCACCGAGCTCCTGGCCGAGATGCTTGCCGTGTCGCCGGCGATGTGCCCCGACATTGAGGACCGCGCCGCGGTGGCCGGCGATGCTGGCATGGAGGTCGTCGGGATGTCGTCGGACGACATGGATCAGTTCGACTTCTCCGTCGATGACATAGACTTCGGGGACTTCTTCCTGAGGCTGGAGGACGGTGATGTGCTCCCGGACCTCGAGGTCGACCCGGCCGAGATCTTCACCGACTTCGAGGCAATCGCGACGAGTGGAGGCGAAGGTGTGCAGGACCAGGAGGTGCCCACCGTCGAGCTCTTGGCGCCTGCGGACGACGTCGGTGTGCTGGATCCGTGCGGCGATGTCGTCGTCGGGGAGGAGAACGCGGCGTTTGCCGGGGCTGGAGAGGAGAAGGGAGGGTGTAACCAGGACGATGATGCGGGGGAAGCGAATGTCGACGATGGAGCCGCGGCGGTTGAGGCCAAGTCTTCGTCGCCGTCATCGACGACGTCGTCGTCGCAGGAGGCTGAGAGCCGGCACAAGTCATCCAGCAAGAGCTCCCATGGGAAGAAGAAAGCGAAGGTGGACTGGACGCCTGAGCTTCACCGGAGGTTCGTGCAGGCGGTGGAGCAGCTCGGCATCGACAAGGCCGTGCCGTCGAGGATACTTGAGATCATGGGGATCGACTCTCTCACCCGGCACAACATAGCCAGCCATCTTCAGAAGTACCGGTCACACAGAAAACACATGATTGCGAGAGAGGCGGAGGCAGCGAGTTGGACCCAACGGCGGCAGATTTACGCCGCCGGTGGAGGTGCTGTTGCGAAGAGGCCGGAGTCCAACGCGTGGACCGTGCCAACCATTGGCTTCCCTCCTCCTCCGCCACCACCACCATCACCGGCTCCGATTCAACATTTTGCTCGCCCGTTGCATGTTTGGGGCCACCCGACGATGGACCCGTCCCGAGTTCCAGTGTGGCCACCGCGGCACCTCGTTCCCCGTGGCCCGGCGCCACCATGGGTTCCACCGCCGCCGCCGTCGGACCCTGCTTTCTGGCACCACCCTTACATGAGGGGGCCAGCACATGTGCCAACTCAAGGGACACCTTGCATGGCGATGCCCATGCCAGCTGCGAGATTTCCTGCTCCACCGGTGCCAGGAGTTGTCCCGTGTCCAATGTATAGGCCATTGACTCCACCAGCACTGGCGAGCAAGAATCAGCAGGACGCACAGCTTCAACTCCAGGTTCAACCATCAAGCGAGAGCATCGACGCAGCTATCGGTGATGTTTTATCGAAACCGTGGTTGCCTTTGCCTCTTGGACTGAAGCCACCTTCAGTGGACAGTGTGATGGGCGAGCTGCAGAGGCAAGGCGTAGCAAACGTTCCTCCAGCGTGTGGATGATATTTGCATGATAGATTGATGGTTCCGTTACTGACTGATTGCTTATCTGGTCAGTTCACCAAAAAATGGGAAAATTCGCCGACATGTCCTTTTATTTTTCTTTTGGCCTAGCGTTTCTTGGACATCTCGTTTAATTTTTAACTTGTGCAGAGTTCGAAAGACATCTTTGTTTCGACTCCGGGAGAAATTAACCTGCGTATTGTAAATGTAAAATGTATTGGCCGTGAGTTCTCAGCAGCTAAGAATAAATTAAATAATGGTCCTCCGAGTAGGGTTTACTTGATTTTGCGTGTTGGTGTTGGTTCAAACGCTCTAATATCTAAATTCCGCATTGTACTATTTTCACAAAGGAAAGTAGCCCCCGCAATTGCACAGGCTTCTT
CDS Sequence
  • >LOC_Os06g24070.1
    ATGCTTGCCGTGTCGCCGGCGATGTGCCCCGACATTGAGGACCGCGCCGCGGTGGCCGGCGATGCTGGCATGGAGGTCGTCGGGATGTCGTCGGACGACATGGATCAGTTCGACTTCTCCGTCGATGACATAGACTTCGGGGACTTCTTCCTGAGGCTGGAGGACGGTGATGTGCTCCCGGACCTCGAGGTCGACCCGGCCGAGATCTTCACCGACTTCGAGGCAATCGCGACGAGTGGAGGCGAAGGTGTGCAGGACCAGGAGGTGCCCACCGTCGAGCTCTTGGCGCCTGCGGACGACGTCGGTGTGCTGGATCCGTGCGGCGATGTCGTCGTCGGGGAGGAGAACGCGGCGTTTGCCGGGGCTGGAGAGGAGAAGGGAGGGTGTAACCAGGACGATGATGCGGGGGAAGCGAATGTCGACGATGGAGCCGCGGCGGTTGAGGCCAAGTCTTCGTCGCCGTCATCGACGACGTCGTCGTCGCAGGAGGCTGAGAGCCGGCACAAGTCATCCAGCAAGAGCTCCCATGGGAAGAAGAAAGCGAAGGTGGACTGGACGCCTGAGCTTCACCGGAGGTTCGTGCAGGCGGTGGAGCAGCTCGGCATCGACAAGGCCGTGCCGTCGAGGATACTTGAGATCATGGGGATCGACTCTCTCACCCGGCACAACATAGCCAGCCATCTTCAGAAGTACCGGTCACACAGAAAACACATGATTGCGAGAGAGGCGGAGGCAGCGAGTTGGACCCAACGGCGGCAGATTTACGCCGCCGGTGGAGGTGCTGTTGCGAAGAGGCCGGAGTCCAACGCGTGGACCGTGCCAACCATTGGCTTCCCTCCTCCTCCGCCACCACCACCATCACCGGCTCCGATTCAACATTTTGCTCGCCCGTTGCATGTTTGGGGCCACCCGACGATGGACCCGTCCCGAGTTCCAGTGTGGCCACCGCGGCACCTCGTTCCCCGTGGCCCGGCGCCACCATGGGTTCCACCGCCGCCGCCGTCGGACCCTGCTTTCTGGCACCACCCTTACATGAGGGGGCCAGCACATGTGCCAACTCAAGGGACACCTTGCATGGCGATGCCCATGCCAGCTGCGAGATTTCCTGCTCCACCGGTGCCAGGAGTTGTCCCGTGTCCAATGTATAGGCCATTGACTCCACCAGCACTGGCGAGCAAGAATCAGCAGGACGCACAGCTTCAACTCCAGGTTCAACCATCAAGCGAGAGCATCGACGCAGCTATCGGTGATGTTTTATCGAAACCGTGGTTGCCTTTGCCTCTTGGACTGAAGCCACCTTCAGTGGACAGTGTGATGGGCGAGCTGCAGAGGCAAGGCGTAGCAAACGTTCCTCCAGCGTGTGGATGA
Protein Sequence
  • >LOC_Os06g24070.1
    MLAVSPAMCPDIEDRAAVAGDAGMEVVGMSSDDMDQFDFSVDDIDFGDFFLRLEDGDVLPDLEVDPAEIFTDFEAIATSGGEGVQDQEVPTVELLAPADDVGVLDPCGDVVVGEENAAFAGAGEEKGGCNQDDDAGEANVDDGAAAVEAKSSSPSSTTSSSQEAESRHKSSSKSSHGKKKAKVDWTPELHRRFVQAVEQLGIDKAVPSRILEIMGIDSLTRHNIASHLQKYRSHRKHMIAREAEAASWTQRRQIYAAGGGAVAKRPESNAWTVPTIGFPPPPPPPPSPAPIQHFARPLHVWGHPTMDPSRVPVWPPRHLVPRGPAPPWVPPPPPSDPAFWHHPYMRGPAHVPTQGTPCMAMPMPAARFPAPPVPGVVPCPMYRPLTPPALASKNQQDAQLQLQVQPSSESIDAAIGDVLSKPWLPLPLGLKPPSVDSVMGELQRQGVANVPPACG*