Gene Details:

Functional Descriptions:

  • We characterized the function of two rice phosphate (Pi) transporters: OsPHT1;9 (OsPT9) and OsPht1;10 (OsPT10).
  • Conversely, although no alterations in Pi concentration were found in OsPT9 or OsPT10 knockdown plants, a significant reduction in Pi concentration in both shoots and roots was observed in double-knockdown plants grown under both high- and low-Pi conditions.
  • Moreover, expression of OsPT2, which encodes a low-affinity Pi transporter, was up-regulated in OsMYB2P-1-overexpressing plants under Pi-sufficient conditions, whereas expression of the high-affinity Pi transporters OsPT6, OsPT8, and OsPT10 was up-regulated by overexpression of OsMYB2P-1 under Pi-deficient conditions, suggesting that OsMYB2P-1 may act as a Pi-dependent regulator in controlling the expression of Pi transporters.
  • OsPT9 and OsPT10 were expressed in the root epidermis, root hairs and lateral roots, with their expression being specifically induced by Pi starvation.
  • High-affinity Km values for Pi transport of OsPT9 and OsPT10 were determined by yeast experiments and two-electrode voltage clamp analysis of anion transport in Xenopus oocytes expressing OsPT9 and OsPT10.
  • Pi uptake and Pi concentrations in transgenic plants harbouring overexpressed OsPT9 and OsPT10 were determined by Pi concentration analysis and (33) P-labelled Pi uptake rate analysis.
  • Taken together, our results suggest that OsPT9 and OsPT10 redundantly function in Pi uptake.
  • Phosphate transporters OsPHT1;9 and OsPht1;10 are involved in phosphate uptake in rice.

Literature:

Gene Resources:

Sequences:

cDNA Sequence
  • >LOC_Os06g21950.1
    ATGGCTCCGATCGGCGTGCTCACGGCGCTGGACCAGGCGCGGACGCAGTACTACCACTTCAAGGCCATCGTCATCGCCGGCATGGGACTCTTCACCGACTCCTACGACCTCTTCTGCATCGCGCCCGTCATGAAGATCGTGGGCCGGGTGTACTACTCCGACGGCGGCGCCCGTCCGGGGGTGACGCCGCCGGCCGTCGTGTCGGCGACCGTCGGAGTGGCGCTGCTGGGCGCGGTGATCGGGAACGTGGTGTTCGGCGCGCTGGGCGACCGCGTGGGGCGGCGCCGCGTCTACGGCGCGTGCCTGCTGCTGATGGTGTGCAGCTCCGTCGGGAGCGGCTTCTCCGTGTGCCGGACGCGCCGGTGCGCGCTCGCCAGCCTCTGCTTCTTCCGCTTCCTCCTCGGCGTCGGCGTCGGCGGCGACTACCCGCTGTCGGCCACCATCATGTCGGAGTTCGCCAACCGGCGCACCCGCGGCGCGTTCATCGCCGCCGTGTTCTCCATGCAGGGGTTCGGCATCCTCGCCAGCTCCGCGGTCACCATGGCCGTCGCCGCCGCCTTCGACCACTACACCGGCTACCCCGCGCCGCTCGACACGCCGGAGTGCGCCGACCTCGCCTGGCGGATCATTCTCATGGCCGGCGCCGTCCCCGCCGCGCTGACGTACTACTGGAGGATGTCCATGCCGGAGACGGCCAGATATACGGCTTTGGTGGAGCGCGACGTGGTGAAGGCCACCAACGACATCGGCCGCGTTCTGGCCGACCTCGACCTGGGCGCCGTCGCCGAGGAGGAGGTGGCGGCGGCGTTGAGCCGGCCTCCGCCGCCGCCGCGCCCTTCGTACGGCCTCCTCTCTCGGCGCTTCGTCCGGCAGCACGGGCGGGACCTGTTCGCGTGCGCGGCGGCGTGGTTCCTCCTCGACATCCCCTACTACAGCAGCACGCTGTTCCAGTCTCAGATCTACCGGCCGCTGTTCCCGGCGCCGGGGCTCATCAACGCGTTCCAGGAGGCGTTCAACGTGGCCAAGTTCCAGGCCGTCATCGCCGTCGCCTCCACCATCCCGGGCTACTTCGTCGCCGTGCTCCTCATCGACCGCGTCGGCCGGCGGTGCCTCCAGATGGCCGGGTTCCTCCTCATGGCCGTCTTCCTCTTCGCGCTCGCGGGTCCGTACGACGGATACTGGCGCGACCACGGCGCGCACGCCGGCTACATCGTGCTCTACTCGCTCACCTTCTTCTCCGCCAACCTCGGGCCGAACACCACCACCTTCATCCTGCCGGCGGAGCTCTTCCCGGCGCGCTTCCGGTCGACGTGCCACGGCCTCTCCGGCGCGGCGGGGAAGCTCGGCGCGCTCGTCGGCTCCATCGGCTTCCTGTGGGCGTCGCAGCAGAAGGACGGCGCGGCGGCCGGGCACCTGCCGGGCATCGGCATGATGTACGCGCTGTTCGTTCTTGGCGGGATCTGCCTGCTCGGGCTGGCACTCACCTACGTGTTCACGCCGGAGACGATGATGCGGTCGCTGGAGGAGAACGAGAGCGACAGGGCGCAGACCCAGGTCGGCGACGGCGGCAGCGACACCGAGGCGGCCAAGAGCCCGGCGTCCATGGCGAGCTCGCACCTCAGCATGTCGCCCATCCTTCCAGCCCGGGTCTCCGTGTGAGTGAACTCGATCATCGTCGGAAAGCGAGAGTGCTTTTCCTCACTATTTGTGCGTCAGAGAGAGTGCATGCTGTCGAGATGTCGATGCATGCATAAGCATGACTTGTGTACAATCTCATGGACTCACACTATATGCAAATACATGTAGGATATGCATATTCAGTTTTGCTAGCAATGCATGTTTCTGATGTTGGCCATAAACCCTTAATCTACACTCACGCGAGTAGTGGACAATGCCTGAACAACGGACGAACACCTTGCAAGCACTGAAAATACCTTGACGATGAACACGAGTTTTGGCAACGCGTAACTTGCCGGCTTTTCTGTTTCA
CDS Sequence
  • >LOC_Os06g21950.1
    ATGGCTCCGATCGGCGTGCTCACGGCGCTGGACCAGGCGCGGACGCAGTACTACCACTTCAAGGCCATCGTCATCGCCGGCATGGGACTCTTCACCGACTCCTACGACCTCTTCTGCATCGCGCCCGTCATGAAGATCGTGGGCCGGGTGTACTACTCCGACGGCGGCGCCCGTCCGGGGGTGACGCCGCCGGCCGTCGTGTCGGCGACCGTCGGAGTGGCGCTGCTGGGCGCGGTGATCGGGAACGTGGTGTTCGGCGCGCTGGGCGACCGCGTGGGGCGGCGCCGCGTCTACGGCGCGTGCCTGCTGCTGATGGTGTGCAGCTCCGTCGGGAGCGGCTTCTCCGTGTGCCGGACGCGCCGGTGCGCGCTCGCCAGCCTCTGCTTCTTCCGCTTCCTCCTCGGCGTCGGCGTCGGCGGCGACTACCCGCTGTCGGCCACCATCATGTCGGAGTTCGCCAACCGGCGCACCCGCGGCGCGTTCATCGCCGCCGTGTTCTCCATGCAGGGGTTCGGCATCCTCGCCAGCTCCGCGGTCACCATGGCCGTCGCCGCCGCCTTCGACCACTACACCGGCTACCCCGCGCCGCTCGACACGCCGGAGTGCGCCGACCTCGCCTGGCGGATCATTCTCATGGCCGGCGCCGTCCCCGCCGCGCTGACGTACTACTGGAGGATGTCCATGCCGGAGACGGCCAGATATACGGCTTTGGTGGAGCGCGACGTGGTGAAGGCCACCAACGACATCGGCCGCGTTCTGGCCGACCTCGACCTGGGCGCCGTCGCCGAGGAGGAGGTGGCGGCGGCGTTGAGCCGGCCTCCGCCGCCGCCGCGCCCTTCGTACGGCCTCCTCTCTCGGCGCTTCGTCCGGCAGCACGGGCGGGACCTGTTCGCGTGCGCGGCGGCGTGGTTCCTCCTCGACATCCCCTACTACAGCAGCACGCTGTTCCAGTCTCAGATCTACCGGCCGCTGTTCCCGGCGCCGGGGCTCATCAACGCGTTCCAGGAGGCGTTCAACGTGGCCAAGTTCCAGGCCGTCATCGCCGTCGCCTCCACCATCCCGGGCTACTTCGTCGCCGTGCTCCTCATCGACCGCGTCGGCCGGCGGTGCCTCCAGATGGCCGGGTTCCTCCTCATGGCCGTCTTCCTCTTCGCGCTCGCGGGTCCGTACGACGGATACTGGCGCGACCACGGCGCGCACGCCGGCTACATCGTGCTCTACTCGCTCACCTTCTTCTCCGCCAACCTCGGGCCGAACACCACCACCTTCATCCTGCCGGCGGAGCTCTTCCCGGCGCGCTTCCGGTCGACGTGCCACGGCCTCTCCGGCGCGGCGGGGAAGCTCGGCGCGCTCGTCGGCTCCATCGGCTTCCTGTGGGCGTCGCAGCAGAAGGACGGCGCGGCGGCCGGGCACCTGCCGGGCATCGGCATGATGTACGCGCTGTTCGTTCTTGGCGGGATCTGCCTGCTCGGGCTGGCACTCACCTACGTGTTCACGCCGGAGACGATGATGCGGTCGCTGGAGGAGAACGAGAGCGACAGGGCGCAGACCCAGGTCGGCGACGGCGGCAGCGACACCGAGGCGGCCAAGAGCCCGGCGTCCATGGCGAGCTCGCACCTCAGCATGTCGCCCATCCTTCCAGCCCGGGTCTCCGTGTGA
Protein Sequence
  • >LOC_Os06g21950.1
    MAPIGVLTALDQARTQYYHFKAIVIAGMGLFTDSYDLFCIAPVMKIVGRVYYSDGGARPGVTPPAVVSATVGVALLGAVIGNVVFGALGDRVGRRRVYGACLLLMVCSSVGSGFSVCRTRRCALASLCFFRFLLGVGVGGDYPLSATIMSEFANRRTRGAFIAAVFSMQGFGILASSAVTMAVAAAFDHYTGYPAPLDTPECADLAWRIILMAGAVPAALTYYWRMSMPETARYTALVERDVVKATNDIGRVLADLDLGAVAEEEVAAALSRPPPPPRPSYGLLSRRFVRQHGRDLFACAAAWFLLDIPYYSSTLFQSQIYRPLFPAPGLINAFQEAFNVAKFQAVIAVASTIPGYFVAVLLIDRVGRRCLQMAGFLLMAVFLFALAGPYDGYWRDHGAHAGYIVLYSLTFFSANLGPNTTTFILPAELFPARFRSTCHGLSGAAGKLGALVGSIGFLWASQQKDGAAAGHLPGIGMMYALFVLGGICLLGLALTYVFTPETMMRSLEENESDRAQTQVGDGGSDTEAAKSPASMASSHLSMSPILPARVSV*