Pi9;Piz-t

Gene Details

MSU gene ID: LOC_Os06g17900
RAPdb gene ID: Os06g0286700
Gene Symbol: Pi9 Piz-t Pi2
Genome: MSU7 , IRGSP-1.0
Species: Oryza sativa

Functional Descriptions

The broad-spectrum rice blast resistance gene Pi9 was cloned using a map-based cloning strategy.
The cloned Pi9 gene provides a starting point to elucidate the molecular basis of the broad-spectrum disease resistance and the evolutionary mechanisms of blast resistance gene clusters in rice.
The broad-spectrum blast resistance gene Pi9 encodes a nucleotide-binding site-leucine-rich repeat protein and is a member of a multigene family in rice.
Semiquantitative reverse transcriptase (RT)-PCR analysis showed that Pi9 was constitutively expressed in the Pi9-resistant plants and was not induced by blast infection.
Disease evaluation of the transgenic lines carrying the individual candidate genes confirmed that Nbs2-Pi9 is the Pi9 gene.
Molecular breeding of thermo-sensitive genic male sterile (TGMS) lines of rice for blast resistance using Pi2 gene.
In this study, we report the introgression of a rice blast resistance gene Pi2 from VE6219 into C815S, an elite rice TGMS line, leading to the development of blast resistant TGMS lines through marker assisted selection (MAS) and phenotypic selection approaches.
Four new TGMS lines with blast resistance gene Pi2 were developed from C815S (an elite TGMS line susceptible to the blast, used as recurrent parent) and VE6219 (a blast resistant line harboring Pi2, used as donor parent).
The broad-spectrum and durable rice blast resistant gene Pi2 was introgressed into the elite TGMS line C815S background.
Allele-specific marker-based assessment revealed that the rice blast resistance genes Pi2 and Pi9 have not been widely deployed in Chinese indica rice cultivars.
The Pi2 and Pi9 genes confer broad-spectrum resistance against diverse blast isolates.
Development of allele-specific markers for Pi2 and Pi9 would facilitate breeding of blast resistant rice by using the two blast resistance genes.
Of the 434 accessions tested, while one indica restorer line Huazhan was identified harboring the Pi2 resistance allele, no other rice line was identified harboring the Pi2 or Pi9 resistance alleles.
The two newly developed markers should be highly useful for using Pi2 and Pi9 in marker-assisted selection (MAS) breeding programs.
In this work, we developed two new markers, named Pi9-Pro and Pi2-LRR respectively, targeting the unique polymorphisms of the resistant and susceptible alleles of Pi2 and of Pi9.
Allelic variants of the broad-spectrum blast resistance gene, Pi9 (nucleotide binding site-leucine-rich repeat region) have been analyzed in Indian rice landraces.
Six of them were further selected on the basis of their resistance and susceptible pattern for virulence analysis and selective pattern study of Pi9 gene.
All the blast resistance Pi9 alleles were grouped into one cluster, whereas Pi9 alleles of susceptible landraces formed another cluster even though these landraces have a low level of DNA polymorphisms.

Functional Keywords

Literature and News

Gene Resources

Sequences

cDNA Sequence

>LOC_Os06g17900.1
ATGCAGGCATTCCTTAGAGCTGCTGAAGTTATGAAAAAGAAAGATGAACTATTAAAGTTGGTGAAACTTAGAGAGCGCCACCGGATCGCTATCCGTATCCACAACCTCAAATCAAGAGTTGAAGAAGTGAGTAGCAGGAACACACGCTACAATTTAGTCGAGCCTATTTCCTCCGGCACAGAGGATGACATGGATTCCTATGCAGAAGACATTCGCAATCAATCAGCTCGAAATGTGGATGAAGCTGAGCTTGTTGGGTTTTCTGACTCCAAGAAAAGGCTGCTTGAAATGATCGATACCAATGCTAATGATGGTCCGGCCAAGGTAATCTGTGTTGTTGGGATGGGTGGTTTAGGCAAGACAGCTCTTTCGAGGAAGATCTTTGAAAGCGAAGAAGACATTAGGAAGAACTTCCCTTGCAATGCTTGGATTACAGTGTCACAATCATTTCACAGGATTGAGCTACTTAAAGATATGATACGCCAACTTCTTGGCCCCAGTTCTCTGGATCAACTCTTGCAAGAATTGCAAGGGAAGGTGGTGGTGCAAGTACATCATCTTTCTGAGTACCTGATAGAAGAGCTCAAGGAGAAGAGGTACTTTGTTGTTCTAGATGATCTATGGATTTTACATGATTGGAATTGGATAAATGAAATTGCATTTCCTAAGAACAATAAGAAGGGCAGTCGAATAGTAATAACCACTCGGAATGTTGATCTTGCGGAGAAGTGTGCCACAGCCTCACTGGTGTACCACCTTGATTTCTTGCAGATGAACGATGCCATAACATTGCTACTGAGAAAAACAAATAAAAATCATGAAGACATGGAATCAAATAAAAATATGCAAAAGATGGTTGAACGAATTGTAAATAAATGTGGTCGTCTACCATTAGCAATACTTACAATAGGAGCTGTGCTTGCAACTAAACAGGTGTCAGAATGGGAGAAATTCTATGAACACCTTCCTTCAGAACTAGAAATAAACCCAAGCCTGGAAGCTTTGAGGAGAATGGTGACCCTAGGTTACAACCACCTACCATCCCATCTGAAACCATGCTTTTTGTATCTAAGTATCTTTCCTGAGGATTTTGAAATCAAAAGGAATCGTCTAGTAGGTAGATGGATAGCAGAAGGGTTTGTTAGACCAAAGGTTGGGATGACGACTAAGGATGTCGGAGAAAGTTACTTTAATGAGCTAATCAACCGAAGTATGATTCAACGATCAAGAGTGGGCATAGCAGGAAAAATTAAGACTTGTCGAATTCATGATATCATCCGTGATATCACAGTTTCAATCTCGAGACAGGAAAATTTTGTATTGTTACCAATGGGAGATGGCTCTGATTTAGTTCAGGAAAACACTCGCCACATAGCATTCCATGGGAGTATGTCCTGCAAAACAGGATTGGATTGGAGCATTATTCGATCATTAGCTATTTTTGGTGACAGACCCAAGAGTCTAGCACATGCAGTTTGTCCAGATCAATTGAGGATGTTACGGGTCTTGGATCTTGAAGATGTGACATTCTTAATCACTCAAAAAGATTTCGACCATATTGCATTGTTGTGCCACTTGAAATACTTGAGTATTGGATATTCGTCATCCATATATTCACTTCCCAGATCCATTGGTAAACTACAGGGCCTACAAACTTTGAACATGCCGAGCACATACATTGCAGCACTACCAAGTGAGATCAGTAAACTCCAATGTCTGCATACTCTTCGTTGTATAGGACAGTTTCATTATGACAACTTTAGTCTAAACCACCCAATGAAGTGCATAACTAACACAATATGCCTGCCTAAAGTATTCACACCTTTAGTTAGTCGCGATGATCGTGCAAAACAAATTGCTGAATTGCACATGGCCACCAAAAGTTGCTGGTCTGAATCATTCGGTGTGAAGGTACCCAAAGGAATAGGTAAGTTGCGAGACTTACAGGTTCTAGAGTATGTAGATATCAGGCGGACCAGTAGTAGAGCAATCAAAGAGCTGGGGCAGTTAAGCAAGCTGAGGAAATTAGGTGTGACAACAAACGGGTCGACAAAGGAAAAATGTAAGATACTTTATGCAGCCATTGAGAAGCTCTCTTCCCTCCAATCTCTCCATGTGGATGCTGTGTTATTCTCAGGTATTATTGGAACACTTGAGTGCCTAGATTCTATTTCATCTCCTCCTCCCCTACTAAGGACACTCAGGTTGAATGGAAGTCTTGAAGAGATGCCTAACTGGATTGAGCAGCTCACTCACCTGAAGAAGTTCGACTTACGGAGGAGTAAACTAAAGGAAGGTAAAACCATGCTGATACTTGGGGCATTGCCCAACCTCATGGTCCTTTATCTTTATCGGAATGCTTACCTTGGGGAGAAGCTAGTATTCAAAACGGGAGCATTCCCAAATCTTAGAACACTTTGTATTTACGAATTGGATCAGCTAAGAGAGATCAGATTTGAGGACGGCAGCTCACCCCTGTTGGAAAAGATAGAAATAGGCAAGTGCAGGTTGGAATCTGGGATTATTGGTATCATTCACCTTCCAAAGCTCAAGGAGATTCCAATTACATACGGAAGTAAAGTGGCTGGGCTTGGTCAGCTGGAGGGAGAAGTGAACACACACCCAAATCGCCCCGTGCTGCTAATGTACAGTGACCGAAGGTATCACGACCTGGGGGCTGAAGCCGAAGGATCTTCTATAGAAGTGCAAACAGCAGATCCTGTTCCTGATGCCGAAGGATCAGTCACTGTAGCAGTGGAAGCAACGGATCCCCTTCCCGAGCAGGAGGGAGAGAGCTCGCAGTCGCAGGTGATCACGTTGACGACGAATGATAGGTCAGTCACTCCCTACATGGCAGCTTAA

CDS Sequence

>LOC_Os06g17900.1
ATGCAGGCATTCCTTAGAGCTGCTGAAGTTATGAAAAAGAAAGATGAACTATTAAAGTTGGTGAAACTTAGAGAGCGCCACCGGATCGCTATCCGTATCCACAACCTCAAATCAAGAGTTGAAGAAGTGAGTAGCAGGAACACACGCTACAATTTAGTCGAGCCTATTTCCTCCGGCACAGAGGATGACATGGATTCCTATGCAGAAGACATTCGCAATCAATCAGCTCGAAATGTGGATGAAGCTGAGCTTGTTGGGTTTTCTGACTCCAAGAAAAGGCTGCTTGAAATGATCGATACCAATGCTAATGATGGTCCGGCCAAGGTAATCTGTGTTGTTGGGATGGGTGGTTTAGGCAAGACAGCTCTTTCGAGGAAGATCTTTGAAAGCGAAGAAGACATTAGGAAGAACTTCCCTTGCAATGCTTGGATTACAGTGTCACAATCATTTCACAGGATTGAGCTACTTAAAGATATGATACGCCAACTTCTTGGCCCCAGTTCTCTGGATCAACTCTTGCAAGAATTGCAAGGGAAGGTGGTGGTGCAAGTACATCATCTTTCTGAGTACCTGATAGAAGAGCTCAAGGAGAAGAGGTACTTTGTTGTTCTAGATGATCTATGGATTTTACATGATTGGAATTGGATAAATGAAATTGCATTTCCTAAGAACAATAAGAAGGGCAGTCGAATAGTAATAACCACTCGGAATGTTGATCTTGCGGAGAAGTGTGCCACAGCCTCACTGGTGTACCACCTTGATTTCTTGCAGATGAACGATGCCATAACATTGCTACTGAGAAAAACAAATAAAAATCATGAAGACATGGAATCAAATAAAAATATGCAAAAGATGGTTGAACGAATTGTAAATAAATGTGGTCGTCTACCATTAGCAATACTTACAATAGGAGCTGTGCTTGCAACTAAACAGGTGTCAGAATGGGAGAAATTCTATGAACACCTTCCTTCAGAACTAGAAATAAACCCAAGCCTGGAAGCTTTGAGGAGAATGGTGACCCTAGGTTACAACCACCTACCATCCCATCTGAAACCATGCTTTTTGTATCTAAGTATCTTTCCTGAGGATTTTGAAATCAAAAGGAATCGTCTAGTAGGTAGATGGATAGCAGAAGGGTTTGTTAGACCAAAGGTTGGGATGACGACTAAGGATGTCGGAGAAAGTTACTTTAATGAGCTAATCAACCGAAGTATGATTCAACGATCAAGAGTGGGCATAGCAGGAAAAATTAAGACTTGTCGAATTCATGATATCATCCGTGATATCACAGTTTCAATCTCGAGACAGGAAAATTTTGTATTGTTACCAATGGGAGATGGCTCTGATTTAGTTCAGGAAAACACTCGCCACATAGCATTCCATGGGAGTATGTCCTGCAAAACAGGATTGGATTGGAGCATTATTCGATCATTAGCTATTTTTGGTGACAGACCCAAGAGTCTAGCACATGCAGTTTGTCCAGATCAATTGAGGATGTTACGGGTCTTGGATCTTGAAGATGTGACATTCTTAATCACTCAAAAAGATTTCGACCATATTGCATTGTTGTGCCACTTGAAATACTTGAGTATTGGATATTCGTCATCCATATATTCACTTCCCAGATCCATTGGTAAACTACAGGGCCTACAAACTTTGAACATGCCGAGCACATACATTGCAGCACTACCAAGTGAGATCAGTAAACTCCAATGTCTGCATACTCTTCGTTGTATAGGACAGTTTCATTATGACAACTTTAGTCTAAACCACCCAATGAAGTGCATAACTAACACAATATGCCTGCCTAAAGTATTCACACCTTTAGTTAGTCGCGATGATCGTGCAAAACAAATTGCTGAATTGCACATGGCCACCAAAAGTTGCTGGTCTGAATCATTCGGTGTGAAGGTACCCAAAGGAATAGGTAAGTTGCGAGACTTACAGGTTCTAGAGTATGTAGATATCAGGCGGACCAGTAGTAGAGCAATCAAAGAGCTGGGGCAGTTAAGCAAGCTGAGGAAATTAGGTGTGACAACAAACGGGTCGACAAAGGAAAAATGTAAGATACTTTATGCAGCCATTGAGAAGCTCTCTTCCCTCCAATCTCTCCATGTGGATGCTGTGTTATTCTCAGGTATTATTGGAACACTTGAGTGCCTAGATTCTATTTCATCTCCTCCTCCCCTACTAAGGACACTCAGGTTGAATGGAAGTCTTGAAGAGATGCCTAACTGGATTGAGCAGCTCACTCACCTGAAGAAGTTCGACTTACGGAGGAGTAAACTAAAGGAAGGTAAAACCATGCTGATACTTGGGGCATTGCCCAACCTCATGGTCCTTTATCTTTATCGGAATGCTTACCTTGGGGAGAAGCTAGTATTCAAAACGGGAGCATTCCCAAATCTTAGAACACTTTGTATTTACGAATTGGATCAGCTAAGAGAGATCAGATTTGAGGACGGCAGCTCACCCCTGTTGGAAAAGATAGAAATAGGCAAGTGCAGGTTGGAATCTGGGATTATTGGTATCATTCACCTTCCAAAGCTCAAGGAGATTCCAATTACATACGGAAGTAAAGTGGCTGGGCTTGGTCAGCTGGAGGGAGAAGTGAACACACACCCAAATCGCCCCGTGCTGCTAATGTACAGTGACCGAAGGTATCACGACCTGGGGGCTGAAGCCGAAGGATCTTCTATAGAAGTGCAAACAGCAGATCCTGTTCCTGATGCCGAAGGATCAGTCACTGTAGCAGTGGAAGCAACGGATCCCCTTCCCGAGCAGGAGGGAGAGAGCTCGCAGTCGCAGGTGATCACGTTGACGACGAATGATAGGTCAGTCACTCCCTACATGGCAGCTTAA

Protein Sequence

>LOC_Os06g17900.1
MQAFLRAAEVMKKKDELLKLVKLRERHRIAIRIHNLKSRVEEVSSRNTRYNLVEPISSGTEDDMDSYAEDIRNQSARNVDEAELVGFSDSKKRLLEMIDTNANDGPAKVICVVGMGGLGKTALSRKIFESEEDIRKNFPCNAWITVSQSFHRIELLKDMIRQLLGPSSLDQLLQELQGKVVVQVHHLSEYLIEELKEKRYFVVLDDLWILHDWNWINEIAFPKNNKKGSRIVITTRNVDLAEKCATASLVYHLDFLQMNDAITLLLRKTNKNHEDMESNKNMQKMVERIVNKCGRLPLAILTIGAVLATKQVSEWEKFYEHLPSELEINPSLEALRRMVTLGYNHLPSHLKPCFLYLSIFPEDFEIKRNRLVGRWIAEGFVRPKVGMTTKDVGESYFNELINRSMIQRSRVGIAGKIKTCRIHDIIRDITVSISRQENFVLLPMGDGSDLVQENTRHIAFHGSMSCKTGLDWSIIRSLAIFGDRPKSLAHAVCPDQLRMLRVLDLEDVTFLITQKDFDHIALLCHLKYLSIGYSSSIYSLPRSIGKLQGLQTLNMPSTYIAALPSEISKLQCLHTLRCIGQFHYDNFSLNHPMKCITNTICLPKVFTPLVSRDDRAKQIAELHMATKSCWSESFGVKVPKGIGKLRDLQVLEYVDIRRTSSRAIKELGQLSKLRKLGVTTNGSTKEKCKILYAAIEKLSSLQSLHVDAVLFSGIIGTLECLDSISSPPPLLRTLRLNGSLEEMPNWIEQLTHLKKFDLRRSKLKEGKTMLILGALPNLMVLYLYRNAYLGEKLVFKTGAFPNLRTLCIYELDQLREIRFEDGSSPLLEKIEIGKCRLESGIIGIIHLPKLKEIPITYGSKVAGLGQLEGEVNTHPNRPVLLMYSDRRYHDLGAEAEGSSIEVQTADPVPDAEGSVTVAVEATDPLPEQEGESSQSQVITLTTNDRSVTPYMAA*