OsGSR1;GW6

Gene Details

MSU gene ID: LOC_Os06g15620
RAPdb gene ID: Os06g0266800
Gene Symbol: OsGSR1 GW6 OsGASR7
Genome: MSU7 , IRGSP-1.0
Species: Oryza sativa

Functional Descriptions

The yeast two-hybrid assay revealed that OsGSR1 interacts with DIM/DWF1, an enzyme that catalyzes the conversion from 24-methylenecholesterol to campesterol in BR biosynthesis.
These results suggest that OsGSR1 activates BR synthesis by directly regulating a BR biosynthetic enzyme at the post-translational level.
These results demonstrate that OsGSR1 plays important roles in both BR and GA pathways, and also mediates an interaction between the two signaling pathways.
RNA interference (RNAi) transgenic rice plants with reduced OsGSR1 expression show phenotypes similar to plants deficient in BR, including short primary roots, erect leaves and reduced fertility.
OsGSR1 is involved in crosstalk between gibberellins and brassinosteroids in rice.
Here, we demonstrate that OsGSR1, a member of the GAST (GA-stimulated transcript) gene family, is induced by GA and repressed by BR.
Furthermore, OsGSR1 RNAi plants show a reduced sensitivity to GA treatment, an increased expression of the GA biosynthetic gene OsGA20ox2, which is feedback inhibited by GA signaling, and an elevated level of endogenous GA: together, these suggest that OsGSR1 is a positive regulator of GA signaling.
The OsGSR1 RNAi transgenic rice shows a reduced level of endogenous BR, and the dwarf phenotype could be rescued by the application of brassinolide.
GW6 is highly expressed in the young panicle and increases grain width by promoting cell expansion in the spikelet hull.
GW6 is induced by GA and its knockout down-regulates the expression of GA biosynthesis genes and decreases GA contents in the young panicle.
A quantitative trait locus GW6 controls rice grain size and yield through the gibberellin pathway.
Here, we report the cloning and characterization of a QTL, GW6 (GRAIN WIDTH 6), which encodes a gibberellin-regulated GAST family protein and positively regulates grain width and weight.
The knockout of GW6 exhibits reduced grain size and weight, whereas the overexpression of GW6 results in increased grain size and weight.
We found that a natural variation in the cis element CAAT-box in the promoter of GW6 is associated with its expression level and grain width and weight.
44% increase in rice grain yield, indicating that GW6 has great potential to improve grain yield in rice.
Natural variation in OsGASR7 regulates grain length in rice.

Functional Keywords

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