Gene Details:

• MSU gene ID: LOC_Os06g10880
• RAPdb gene ID: Os06g0211200
• Gene Symbol: OsAREB8 OsAREB1 OsbZIP46 OsABF2 ABL1
• Genome: MSU7 ,  IRGSP-1.0
• Species: Oryza sativa

Functional Descriptions:

• The stress-related genes activated by OsbZIP46CA1 are largely different from those activated by the other rice ABF/AREB homologs (such as OsbZIP23), further implying the value of OsbZIP46CA1 in genetic engineering of drought tolerance.
• Constitutive activation of transcription factor OsbZIP46 improves drought tolerance in rice.
• A homozygous T-DNA insertional mutant of OsABF2 is more sensitive to salinity, drought, and oxidative stress compared with wild type plants.
• In addition, the ABL1 mutant is hypersensitive to exogenous indole-3-acetic acid, and some ABRE-containing genes related to auxin metabolism or signaling are altered under ABL1 deficiency, suggesting that ABL1 modulates ABA and auxin responses by directly regulating the ABRE-containing genes.
• Overexpression of the native OsbZIP46 gene increased ABA sensitivity but had no positive effect on drought resistance.
• These results suggest that OsbZIP46 is a positive regulator of ABA signaling and drought stress tolerance of rice depending on its activation.
• Together, it is suggested that a nuclear PP2C protein OsSIPP2C1 negatively regulated by ABL1 is involved in abiotic stress and panicle development in rice.
• A novel nuclear protein phosphatase 2C negatively regulated by ABL1 is involved in abiotic stress and panicle development in rice.
• Due to the dates, OsAREB1 may function as a positive regulator in drought/heat stresses response, but a negative regulator in flowering time in Arabiclopsis.
• OsSIPP2C1 expression was up-regulated by high salt, PEG6000 and exogenous ABA, and enhanced in the ABL1 mutant under normal, salt, or drought condition.
• ABL1 is expressed in various tissues and is induced by the hormones ABA and indole-3-acetic acid and stress conditions including salinity, drought, and osmotic pressure.
• Expression of OsbZIP46 was strongly induced by drought, heat, hydrogen peroxide, and abscisic acid (ABA) treatment; however, it was not induced by salt and cold stresses.
• Overexpression of OsbZIP46CA1 in rice significantly increased tolerance to drought and osmotic stresses.
• The ABL1 deficiency mutant, ABL1, shows suppressed ABA responses, and ABL1 expression in the Arabidopsis abi5 mutant rescued the ABA sensitivity.
• Trdnsgenic Arabidopsis over-expressing OsAREB1 had different responses to ABA and glucose compared to wild-type plants, which suggest OsAREB1 might have a crucial role in these two signaling pathways.
• OsAREB1, an ABRE-binding protein responding to ABA and glucose, has multiple functions in Arabidopsis.
• In our current study, we have isolated and characterized a group A bZIP transcription factor in rice, OsABF2 (Oryza sativa ABA-responsive element binding factor 2).
• The ABRE-binding bZIP transcription factor OsABF2 is a positive regulator of abiotic stress and ABA signaling in rice.
• Here, we report the functional identification of rice (Oryza sativa) ABI5-Like1 (ABL1), which is a basic region/leucine zipper motif transcription factor.
• Collectively, our present results indicate that OsABF2 functions as a transcriptional regulator that modulates the expression of abiotic stress-responsive genes through an ABA-dependent pathway.
• Purification, crystallization and preliminary X-ray analysis of OsAREB8 from rice, a member of the AREB/ABF family of bZIP transcription factors, in complex with its cognate DNA.
• First, OsAREB1 transgenic plants had higher resistance to drought and heat, and OsAREB1 up-regulated the ABA/stress related gene such as RD29A and RD29B.
• In addition, this OsABF2 mutant showed a significantly decreased sensitivity to high levels of ABA at germination and post-germination.
• OsbZIP46 is one member of the third subfamily of bZIP transcription factors in rice (Oryza sativa).
• SiMYB19 overexpression promotes abscisic acid (ABA) accumulation in transgenic rice and upregulates the ABA synthesis gene OsNCED3 and the ABA signal transduction pathway-related genes OsPK1 and OsABF2.
• Further investigation revealed that the transcription factor basic region and leucine zipper 46 (OsbZIP46), involved in ABA signaling, can directly bind to the YUCCA8/rice ethylene-insensitive 7 (OsYUC8/REIN7) promoter to activate its expression, and genetic analysis revealed that OsYUC8/REIN7 is located downstream of OsbZIP46.

Function-related keywords:

• drought-tolerance ,  salinity ,  auxin ,  ABA ,  abiotic-stress ,  flower ,  drought ,  panicle ,  flowering-time ,  salt ,  transcription-factor ,  oxidative ,  drought-resistance ,  cold-stress ,  abscisic-acid ,  signal-transduction

Literature:

• OsAREB1, an ABRE-binding protein responding to ABA and glucose, has multiple functions in Arabidopsis .  DOI:  10.5483/bmbrep.2010.43.1.034 ;  PMID:  20132733
• The ABRE-binding bZIP transcription factor OsABF2 is a positive regulator of abiotic stress and ABA signaling in rice .  DOI:  10.1016/j.jplph.2010.05.008 ;  PMID:  20576316
• Constitutive activation of transcription factor OsbZIP46 improves drought tolerance in rice .  DOI:  10.1104/pp.111.190389 ;  PMID:  22301130
• Identification of cis-acting promoter elements in cold- and dehydration-induced transcriptional pathways in Arabidopsis, rice, and soybean .  DOI:  10.1093/dnares/dsr040 ;  PMID:  22184637
• Genomic survey and gene expression analysis of the basic leucine zipper transcription factor family in rice .  DOI:  10.1104/pp.107.112821 ;  PMID:  18065552
• Rice ABI5-Like1 regulates abscisic acid and auxin responses by affecting the expression of ABRE-containing genes .  DOI:  10.1104/pp.111.173427 ;  PMID:  21546455
• Purification, crystallization and preliminary X-ray analysis of OsAREB8 from rice, a member of the AREB/ABF family of bZIP transcription factors, in complex with its cognate DNA .  DOI:  10.1107/S1744309112009384 ;  PMID:  22505428
• The rice OsDIL gene plays a role in drought tolerance at vegetative and reproductive stages .  DOI:  10.1007/s11103-013-0057-9 ;  PMID:  23686450
• A novel nuclear protein phosphatase 2C negatively regulated by ABL1 is involved in abiotic stress and panicle development in rice .  DOI:  10.1007/s12033-012-9614-8 ;  PMID:  23086454
• The coordination of OsbZIP72 and OsMYBS2 with reverse roles regulates the transcription of OsPsbS1 in rice .  DOI:  10.1111/nph.16877 ;  PMID:  33411361
• SiMYB19 from Foxtail Millet (Setaria italica) Confers Transgenic Rice Tolerance to High Salt Stress in the Field .  DOI:  10.3390/ijms23020756 ;  PMID:  35054940
• Abscisic acid promotes auxin biosynthesis to inhibit primary root elongation in rice .  DOI:  10.1093/plphys/kiac586 ;  PMID:  36535001

Related News:

Gene Resources:

• NCBI ID: AK103188  , AK105312  , GU552783
• UniProt accessions:

Sequences: