Information report for OsERF71
Gene Details
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Functional Descriptions
- Overexpression of the OsERF71 transcription factor Alters Rice Root Structure and Drought Resistance.
- OsERF71 overexpression in roots elevated the expression levels of genes related to cell wall loosening and lignin biosynthetic genes, which correlated with changes in root structure, the formation of enlarged aerenchyma and high lignification levels.
- Overexpression of OsERF71 either throughout the entire plant or specifically in roots, resulted in a drought resistance phenotype at the vegetative growth stage, indicating that overexpression in roots was sufficient to confer drought resistance.
- Furthermore, OsERF71 was found to directly bind to the promoter of OsCCR1, a key gene in lignin biosynthesis.
- Our latest study demonstrated that overexpression of the OsERF71 in rice roots under drought conditions modifies root structure including larger aerenchyma and radial root growth, and thereby, protects the rice plants from drought stresses.
- Using yeast one-hybrid screening, coupled with chromatin immunoprecipitation analysis, a basic helix-loop-helix protein (OsbHLH59) and an APETALA2/ETHYLENE RESPONSE FACTOR (AP2/ERF) transcription factor OsERF71 directly binding to the 562<U+00A0>bp key sequence to activate the expression of OsXIP were identified, which is further supported by transient expression assay.
- Moreover, transcriptional analysis revealed that mechanical wounding and treatment with MeJA resulted in an obvious increase in transcript levels of OsbHLH59 and OsERF71 in root and shoot tissues.
- OsERF71 confers drought tolerance via modulating ABA signaling and proline biosynthesis.
- Overexpression of the OsERF71 in Nipponbare demonstrated a significant increase in tolerance to drought stress and a reduced rate of water loss.
- In contrast, OsERF71 interference lines were sensitive to drought stress and exhibited a higher rate of water loss.
- Moreover, OsERF71 regulated the expression of several ABA- responsive and proline biosynthesis genes under drought stress, resulting in enhanced sensitivity to exogenous ABA treatment and proline accumulation.
- Accordingly, we suggest that OsERF71 plays a positive role in drought stress tolerance by increasing the expression of genes associated with ABA signaling and proline biosynthesis under stress.
- The expression of OsERF71 was induced by abscisic acid (ABA) and various abiotic stresses preferentially in IRAT109 under ABA, dehydration, and polyethyleneglycol (PEG) treatments.
Functional Keywords
- root , growth , resistance , drought , transcription-factor , vegetative , cell-wall , drought-resistance , lignin , lignin-biosynthesis , drought-stress , shoot , wounding , ethylene-response , tolerance , abiotic-stress , ABA , drought-tolerance , stress , biotic-stress , water-loss , abscisic-acid , stress-tolerance
Literature and News
- Overexpression of the OsERF71 Transcription Factor Alters Rice Root Structure and Drought Resistance . DOI: 10.1104/pp.16.00379 ; PMID: 27382137
- Rice OsERF71-mediated root modification affects shoot drought tolerance . DOI: 10.1080/15592324.2016.1268311 ; PMID: 27935412
- Identification of two transcription factors activating the expression of OsXIP in rice defence response . DOI: 10.1186/s12896-017-0344-7 ; PMID: 28270131
- Transcriptional Network Analysis Reveals Drought Resistance Mechanisms of AP2/ERF Transgenic Rice . DOI: 10.3389/fpls.2017.01044 ; PMID: 28663756
- OsERF71 confers drought tolerance via modulating ABA signaling and proline biosynthesis . DOI: 10.1016/j.plantsci.2018.01.017 ; PMID: 29576066
Gene Resources
- UniProt: Q69Y47
- EMBL: AK060742, AK069833, AP014962
- AlphaFoldDB: Q69Y47
- EnsemblPlants: Os06t0194000-01, Os06t0194000-02
- Gramene: Os06t0194000-01, Os06t0194000-02
- KEGG: osa:4340383
- Orthologous matrix: SCDTTQD
- InterPro: IPR001471, IPR016177, IPR036955
- PANTHER: PTHR31190, PTHR31190:SF269
- SUPFAM: SSF54171
- PROSITE: PS51032
- Gene3D: 3.30.730.10
- OrthoDB: Q69Y47
- SWISS-MODEL: Q69Y47
- Conserved Domain Database: cd00018
- eggNOG: ENOG502RYGT
Sequences
cDNA Sequence
- >LOC_Os06g09390.1
ACTCTCTGTAAAAACAAGCAAACAAAGAAAGAAAAGCATCACAAATCTCACGCACTGTCTCTCGTTCGCGCAAAGCACGCTGCTTTTCTCCGCTTTGCGAGCACCATAGCCTAGCCCACCATGTGCGGCGGCGCCATCCTCTCCGACCTCATCCCGCCGCCGCGGCGGGTCACCGCCGGCGACCTCTGGCTGGAGAAGACCAAGAAGCAGCAGCAGCAGAAGAAGAAGAACAAGGGCGCGAGGAGGCTGCCACTGCGCCAAGAGGAGGAGGATGATTTCGAGGCCGACTTCGAGGAGTTCGAGGTGGATTCCGGCGAGTGGGAGGTGGAGTCCGACGCCGACGAGGCCAAGCCGCTCGCCGCGCCCCGGAGCGGCTTCGCTAAAGGTGGATTGAAAAACACTACTGTTGCTGGTGCTGATGGGCCTGCAGCAAGGTCTGCTAAAAGGAAGAGAAAGAACCAATTCAGGGGTATCCGCCAGCGGCCATGGGGCAAATGGGCTGCGGAAATCAGAGATCCTCGCAAAGGTGTCCGCGTCTGGCTTGGCACCTTCAACTCTCCTGAGGAAGCTGCCAGAGCTTATGATGCTGAAGCACGAAGGATTCGAGGCAAGAAGGCCAAGGTCAATTTCCCAGATGGGGCTCCAGTGGCTTCTCAGAGGAGTCATGCTGAGCCCTCCTCCATGAACATGCCTGCTTTCAGCATCGAAGAGAAGCCGGCCGTCATGTCAGCAGGCAACAAAACCATGTACAACACAAATGCTTATGCCTACCCTGCTGTTGAGTACACCTTACAGGAGCCATTTGTGCAGATTCAGAATGTCTCATTTGTTCCTGCAATGAACGCGATTGAGGATACTTTCGTGAACCTGTCCTCTGATCAAGGGAGCAACTCCTTTGGTTGCTCGGACTTTAGCCAGGAGAATGATATCAAGACCCCTGACATAACTTCCATGCTTGCACCGACCATGACAGGTGTTGATGACTCCGCATTCCTCCAGAACAATGCCAGTGATGCAATGGTACCTCCTGTGATGGGGAATGCTAGCATTGATCTTGCTGACCTGGAGCCGTACATGAAATTTCTGATCGATGGTGGTTCGGATGAGTCGATTGACACCCTTCTGAGCTCTGATGGATCTCAGGATGTGGCCAGTAGCATGGACCTTTGGAGCTTCGATGACATGCCCGTGTCGGCCGAGTTCTACTGAGGGGTTTGGGGTGTAGCAACTGGTGCCTGTATATATAAGGACAAATGGAATAAACATTCTGGACATCCAAGAAGCGGCATGTGTCTGTCGGGCGCTTCTAGTTGCGCTATATAGCTATGTTAGTATGTTAGTATGTGCTGTGTCTAGCTTAGATGCTGAAGTCTCAAGTACTATTTGGCAGTGAAACTATCTATCTGTAACTGCTATATGAGGCTGGAACAAGTTACTTAGCTTCTACCTTATCTGTACTTGCTATAGTGGCTGTGAACCTTGTGGATCTGAACTCTGAAGCCAATGTTTACTATATAATGTGGTTGGTTTTATAAACTCTAGTTGATTTGGACCCCTGTCAATGGTCATGCTATGGCTGGGGATAAGAACCATTGATGATCATATTTGACTAGAAACGCTGCTTCCAAAATATCCCATAAAGTAAAGCTGAACTTGTACTAATGTTCTTGCACAAGTTGCAATAATGTTGCCTTATGGAAAAACAGTTTCCGGACC - >LOC_Os06g09390.2
ACTCTCTGTAAAAACAAGCAAACAAAGAAAGAAAAGCATCACAAATCTCACGCACTGTCTCTCGTTCGCGCAAAGCACGCTGCTTTTCTCCGCTTTGCGAGCACCATAGCCTAGCCCACCATGTGCGGCGGCGCCATCCTCTCCGACCTCATCCCGCCGCCGCGGCGGGTCACCGCCGGCGACCTCTGGCTGGAGAAGACCAAGAAGCAGCAGCAGCAGAAGAAGAAGAACAAGGGCGCGAGGAGGCTGCCACTGCGCCAAGAGGAGGAGGATGATTTCGAGGCCGACTTCGAGGAGTTCGAGGTGGATTCCGGCGAGTGGGAGGTGGAGTCCGACGCCGACGAGGCCAAGCCGCTCGCCGCGCCCCGGAGCGGCTTCGCTAAAGGTGGATTGAAAAACACTACTGTTGCTGGTGCTGATGGGCCTGCAGCAAGGTCTGCTAAAAGGAAGAGAAAGAACCAATTCAGGGGTATCCGCCAGCGGCCATGGGGCAAATGGGCTGCGGAAATCAGAGATCCTCGCAAAGGTGTCCGCGTCTGGCTTGGCACCTTCAACTCTCCTGAGGAAGCTGCCAGAGCTTATGATGCTGAAGCACGAAGGATTCGAGGCAAGAAGGCCAAGGTCAATTTCCCAGATGGGGCTCCAGTGGCTTCTCAGAGGAGTCATGCTGAGCCCTCCTCCATGAACATGCCTGCTTTCAGCATCGAAGAGAAGCCGGCCGTCATGTCAGCAGGCAACAAAACCATGTACAACACAAATGCTTATGCCTACCCTGCTGTTGAGTACACCTTACAGGAGCCATTTGTGCAGATTCAGAATGTCTCATTTGTTCCTGCAATGAACGCGATTGAGGATACTTTCGTGAACCTGTCCTCTGATCAAGGGAGCAACTCCTTTGGTTGCTCGGACTTTAGCCAGGAGAATGATATCAAGACCCCTGACATAACTTCCATGCTTGCACCGACCATGACAGGTGTTGATGACTCCGCATTCCTCCAGAACAATGCCAGTGATGCAATGGTACCTCCTGTGATGGGGAATGCTAGCATTGATCTTGCTGACCTGGAGCCGTACATGAAATTTCTGATCGATGGTGGTTCGGATGAGTCGATTGACACCCTTCTGAGCTCTGATGGATCTCAGGATGTGGCCAGTAGCATGGACCTTTGGAGCTTCGATGACATGCCCGTGTCGGCCGAGTTCTACTGAGGGGTTTGGGGTGTAGCAACTGGTGCCTGTATATATAAGGACAAATGGAATAAACATTCTGGACATCCAAGAAGCGGCATGTGTCTGTCGGGCGCTTCTAGTTGCGCTATATAGCTATGTTAGTATGTTAGTATGTGCTGTGTCTAGCTTAGATGCTGAAGTCTCAAGTACTATTTGGCAGTGAAACTATCTATCTGTAACTGCTATATGAGGCTGGAACAAGTTACTTAGCTTCTACCTTATCTGTACTTGCTATAGTGGCTGTGAACCTTGTGGATCTGAACTCTGAAGCCAATGTTTACTATATAATGTGGTTGGTTTTATAAACTCTAGTTGATTTGGACCCCTGTCAATGGTCATGCTATGGCTGGGGATAAGAACCATTGATGATCATATTTGACTAGAAACGCTGCTTCCAAAATATCCCATAAAGTAAAGCTGAACTTGTACTAATGTTCTTGCACAAGTTGCAATAATGTTGCCTTATGGAAAAACAGTTTCCGGACC
CDS Sequence
- >LOC_Os06g09390.1
ATGTGCGGCGGCGCCATCCTCTCCGACCTCATCCCGCCGCCGCGGCGGGTCACCGCCGGCGACCTCTGGCTGGAGAAGACCAAGAAGCAGCAGCAGCAGAAGAAGAAGAACAAGGGCGCGAGGAGGCTGCCACTGCGCCAAGAGGAGGAGGATGATTTCGAGGCCGACTTCGAGGAGTTCGAGGTGGATTCCGGCGAGTGGGAGGTGGAGTCCGACGCCGACGAGGCCAAGCCGCTCGCCGCGCCCCGGAGCGGCTTCGCTAAAGGTGGATTGAAAAACACTACTGTTGCTGGTGCTGATGGGCCTGCAGCAAGGTCTGCTAAAAGGAAGAGAAAGAACCAATTCAGGGGTATCCGCCAGCGGCCATGGGGCAAATGGGCTGCGGAAATCAGAGATCCTCGCAAAGGTGTCCGCGTCTGGCTTGGCACCTTCAACTCTCCTGAGGAAGCTGCCAGAGCTTATGATGCTGAAGCACGAAGGATTCGAGGCAAGAAGGCCAAGGTCAATTTCCCAGATGGGGCTCCAGTGGCTTCTCAGAGGAGTCATGCTGAGCCCTCCTCCATGAACATGCCTGCTTTCAGCATCGAAGAGAAGCCGGCCGTCATGTCAGCAGGCAACAAAACCATGTACAACACAAATGCTTATGCCTACCCTGCTGTTGAGTACACCTTACAGGAGCCATTTGTGCAGATTCAGAATGTCTCATTTGTTCCTGCAATGAACGCGATTGAGGATACTTTCGTGAACCTGTCCTCTGATCAAGGGAGCAACTCCTTTGGTTGCTCGGACTTTAGCCAGGAGAATGATATCAAGACCCCTGACATAACTTCCATGCTTGCACCGACCATGACAGGTGTTGATGACTCCGCATTCCTCCAGAACAATGCCAGTGATGCAATGGTACCTCCTGTGATGGGGAATGCTAGCATTGATCTTGCTGACCTGGAGCCGTACATGAAATTTCTGATCGATGGTGGTTCGGATGAGTCGATTGACACCCTTCTGAGCTCTGATGGATCTCAGGATGTGGCCAGTAGCATGGACCTTTGGAGCTTCGATGACATGCCCGTGTCGGCCGAGTTCTACTGA - >LOC_Os06g09390.2
ATGTGCGGCGGCGCCATCCTCTCCGACCTCATCCCGCCGCCGCGGCGGGTCACCGCCGGCGACCTCTGGCTGGAGAAGACCAAGAAGCAGCAGCAGCAGAAGAAGAAGAACAAGGGCGCGAGGAGGCTGCCACTGCGCCAAGAGGAGGAGGATGATTTCGAGGCCGACTTCGAGGAGTTCGAGGTGGATTCCGGCGAGTGGGAGGTGGAGTCCGACGCCGACGAGGCCAAGCCGCTCGCCGCGCCCCGGAGCGGCTTCGCTAAAGGTGGATTGAAAAACACTACTGTTGCTGGTGCTGATGGGCCTGCAGCAAGGTCTGCTAAAAGGAAGAGAAAGAACCAATTCAGGGGTATCCGCCAGCGGCCATGGGGCAAATGGGCTGCGGAAATCAGAGATCCTCGCAAAGGTGTCCGCGTCTGGCTTGGCACCTTCAACTCTCCTGAGGAAGCTGCCAGAGCTTATGATGCTGAAGCACGAAGGATTCGAGGCAAGAAGGCCAAGGTCAATTTCCCAGATGGGGCTCCAGTGGCTTCTCAGAGGAGTCATGCTGAGCCCTCCTCCATGAACATGCCTGCTTTCAGCATCGAAGAGAAGCCGGCCGTCATGTCAGCAGGCAACAAAACCATGTACAACACAAATGCTTATGCCTACCCTGCTGTTGAGTACACCTTACAGGAGCCATTTGTGCAGATTCAGAATGTCTCATTTGTTCCTGCAATGAACGCGATTGAGGATACTTTCGTGAACCTGTCCTCTGATCAAGGGAGCAACTCCTTTGGTTGCTCGGACTTTAGCCAGGAGAATGATATCAAGACCCCTGACATAACTTCCATGCTTGCACCGACCATGACAGGTGTTGATGACTCCGCATTCCTCCAGAACAATGCCAGTGATGCAATGGTACCTCCTGTGATGGGGAATGCTAGCATTGATCTTGCTGACCTGGAGCCGTACATGAAATTTCTGATCGATGGTGGTTCGGATGAGTCGATTGACACCCTTCTGAGCTCTGATGGATCTCAGGATGTGGCCAGTAGCATGGACCTTTGGAGCTTCGATGACATGCCCGTGTCGGCCGAGTTCTACTGA
Protein Sequence
- >LOC_Os06g09390.1
MCGGAILSDLIPPPRRVTAGDLWLEKTKKQQQQKKKNKGARRLPLRQEEEDDFEADFEEFEVDSGEWEVESDADEAKPLAAPRSGFAKGGLKNTTVAGADGPAARSAKRKRKNQFRGIRQRPWGKWAAEIRDPRKGVRVWLGTFNSPEEAARAYDAEARRIRGKKAKVNFPDGAPVASQRSHAEPSSMNMPAFSIEEKPAVMSAGNKTMYNTNAYAYPAVEYTLQEPFVQIQNVSFVPAMNAIEDTFVNLSSDQGSNSFGCSDFSQENDIKTPDITSMLAPTMTGVDDSAFLQNNASDAMVPPVMGNASIDLADLEPYMKFLIDGGSDESIDTLLSSDGSQDVASSMDLWSFDDMPVSAEFY* - >LOC_Os06g09390.2
MCGGAILSDLIPPPRRVTAGDLWLEKTKKQQQQKKKNKGARRLPLRQEEEDDFEADFEEFEVDSGEWEVESDADEAKPLAAPRSGFAKGGLKNTTVAGADGPAARSAKRKRKNQFRGIRQRPWGKWAAEIRDPRKGVRVWLGTFNSPEEAARAYDAEARRIRGKKAKVNFPDGAPVASQRSHAEPSSMNMPAFSIEEKPAVMSAGNKTMYNTNAYAYPAVEYTLQEPFVQIQNVSFVPAMNAIEDTFVNLSSDQGSNSFGCSDFSQENDIKTPDITSMLAPTMTGVDDSAFLQNNASDAMVPPVMGNASIDLADLEPYMKFLIDGGSDESIDTLLSSDGSQDVASSMDLWSFDDMPVSAEFY*