Information report for OsMADS5
Gene Details
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Functional Descriptions
- Simultaneous knockdown of the four rice SEP-like genes OsMADS1, OsMADS5, OsMADS7 and OsMADS8, leads to homeotic transformation of all floral organs except the lemma into leaf-like organs.
- In case of the loss-of-function mutation of OsMADS5, no defect in either panicles or vegetative organs was observed.
- Genetic and molecular analyses demonstrated that OsMADS1, OsMADS5 and OsMADS34 together regulate floral meristem determinacy, and specify the identities of spikelet organs by positively regulating the other MADS-box floral homeotic genes.
- Experiments conducted in yeast also suggested that OsMADS1, OsMADS5 and OsMADS34 form protein-protein interactions with other MADS-box floral homeotic members, which seems to be a typical, conserved feature of plant SEP proteins.
- OsMADS5 was expressed strongly across a broad range of reproductive stages and tissues.
- Our results reveal a role for OsMADS5 in panicle development, and show that OsMADS5 and OsMADS34 play similar functions in limiting branching and promoting the transition to spikelet meristem identity, in part by repressing RCN4 expression.
- We have generated loss-of-function and overexpression mutants of the paralogous OsMADS5 and OsMADS34 genes in rice (Oryza sativa), and analysed their panicle phenotypes.
- The OsMADS5 osmads34 mutant lines had significantly enhanced panicle branching with increased secondary, and even tertiary and quaternary, branches, compared to wild-type (WT) and osmads34 plants.
- Rice SEPALLATA genes OsMADS5 and OsMADS34 cooperate to limit inflorescence branching by repressing the TERMINAL FLOWER1-like gene RCN4.
Functional Keywords
- leaf , floral , lemma , vegetative , panicle , spikelet , meristem , reproductive , floral-meristem , floral-meristem-determinacy , spikelet-meristem , inflorescence , branching
Literature and News
- MADS-box gene family in rice: genome-wide identification, organization and expression profiling during reproductive development and stress . DOI: 10.1186/1471-2164-8-242 ; PMID: 17640358
- Functional conservation and diversification of class E floral homeotic genes in rice (Oryza sativa) . DOI: 10.1111/j.1365-313X.2009.04101.x ; PMID: 20003164
- Conservation of the E-function for floral organ identity in rice revealed by the analysis of tissue culture-induced loss-of-function mutants of the OsMADS1 gene . DOI: 10.1007/s11103-005-2161-y ; PMID: 16217607
- Loss of LOFSEP Transcription Factor Function Converts Spikelet to Leaf-Like Structures in Rice . DOI: 10.1104/pp.17.00704 ; PMID: 29217592
- Cloning of long sterile lemma (lsl2), a single recessive gene that regulates spike germination in rice (Oryza sativa L.) . DOI: 10.1186/s12870-020-02776-8 ; PMID: 33308141
- Rice transcription factor MADS32 regulates floral patterning through interactions with multiple floral homeotic genes . DOI: 10.1093/jxb/eraa588 ; PMID: 33337484
- Rice SEPALLATA genes OsMADS5 and OsMADS34 cooperate to limit inflorescence branching by repressing the TERMINAL FLOWER1-like gene RCN4 . DOI: 10.1111/nph.17855 ; PMID: 34767634
Gene Resources
- UniProt: Q0DEB8
- EMBL: AB026295, AK064184, AP008212
- AlphaFoldDB: Q0DEB8
- EnsemblPlants: Os06t0162800-01
- Gramene: Os06t0162800-01
- KEGG: dosa:Os06g0162800
- Orthologous matrix: HASEDNQ
- InterPro: IPR002100, IPR002487, IPR033896
Sequences
cDNA Sequence
- >LOC_Os06g06750.1
CTCCGGCCAGCACAGCCGATGGTACGAGAGCATGGCTAGCTAGCCGAGCTACTTAGCTACTACATCCATGATCCATCCATCCCCAACAAACGGAGCAAGACTGCAAGGGAGAGGGAGAGAGAGGGAAGCTTGCAGGCTGCAGCTAACTAGCTAGGCAAGGAGAGAGAGGAGATAGATCAAGAAGAGATTTTGAGACCGAGAGAGAGCTAGAGAGAGATCGATGGGGCGAGGGAAAGTAGAGCTGAAGCGGATCGAGAACAAGATAAGCCGGCAGGTGACGTTCGCGAAGAGGAGGAACGGGCTGCTGAAGAAGGCGTACGAGCTGTCCGTGCTCTGCGACGCCGAGGTCGCCCTCATCATCTTCTCCACCCGCGGCCGCCTCTTCGAGTTCTCCACCTCCTCCTGTATGTACAAGACACTGGAGCGATACCGCAGTTGCAACTACAACCTTAACTCATGTGAAGCATCTGCTGCACTGGAAACTGAACTAAGCAATTACCAAGAGTACTTAAAGTTAAAGACAAGAGTTGAGTTCCTACAAACAACTCAGAGAAATCTTCTTGGCGAGGACTTGGTTCCACTTAGCTTGAAGGAGCTCGAGCAACTTGAGAACCAGATCGAGATATCCCTCATGAATATCAGGTCATCAAAGAATCAACAGTTGCTTGATCAAGTATTTGAGCTCAAACGTAAGGAACAACAACTTCAAGATGCTAATAAAGACTTAAAAAGGAAGATACAAGAAACTAGTGGAGAAAATATGCTTCATATATCTTGCCAAGATGTAGGGCCCAGTGGCCATGCTAGTGAAGCTAACCAAGAGTTTCTCCATCATGCAATTTGTGACCCTTCCCTGCATATAGGGTATCAAGCTTACATGGATCACCTCAACCAATGAATGAATTGCTTATCACATTAATGGACATCTCCTATGTTGGATGTGGTGTTTGACGTAATGCTCTCTTTTACATGCGGGTTTTACCTTAAGTGTGTGTGCTAAATTTAGTGCGTTTGTTTATGCTCTTTTGAACTGAACAAAGGAATGATCCCGGTTTGATTGATGAATGCTGCAAGAACATAATCTATATGTTAGTCTGAATTCAGTATGTAATGAAGATGTTTTGTTACTAATTAATAAATACGAAGTAAACAATTAACTGACCA
CDS Sequence
- >LOC_Os06g06750.1
ATGGGGCGAGGGAAAGTAGAGCTGAAGCGGATCGAGAACAAGATAAGCCGGCAGGTGACGTTCGCGAAGAGGAGGAACGGGCTGCTGAAGAAGGCGTACGAGCTGTCCGTGCTCTGCGACGCCGAGGTCGCCCTCATCATCTTCTCCACCCGCGGCCGCCTCTTCGAGTTCTCCACCTCCTCCTGTATGTACAAGACACTGGAGCGATACCGCAGTTGCAACTACAACCTTAACTCATGTGAAGCATCTGCTGCACTGGAAACTGAACTAAGCAATTACCAAGAGTACTTAAAGTTAAAGACAAGAGTTGAGTTCCTACAAACAACTCAGAGAAATCTTCTTGGCGAGGACTTGGTTCCACTTAGCTTGAAGGAGCTCGAGCAACTTGAGAACCAGATCGAGATATCCCTCATGAATATCAGGTCATCAAAGAATCAACAGTTGCTTGATCAAGTATTTGAGCTCAAACGTAAGGAACAACAACTTCAAGATGCTAATAAAGACTTAAAAAGGAAGATACAAGAAACTAGTGGAGAAAATATGCTTCATATATCTTGCCAAGATGTAGGGCCCAGTGGCCATGCTAGTGAAGCTAACCAAGAGTTTCTCCATCATGCAATTTGTGACCCTTCCCTGCATATAGGGTATCAAGCTTACATGGATCACCTCAACCAATGA
Protein Sequence
- >LOC_Os06g06750.1
MGRGKVELKRIENKISRQVTFAKRRNGLLKKAYELSVLCDAEVALIIFSTRGRLFEFSTSSCMYKTLERYRSCNYNLNSCEASAALETELSNYQEYLKLKTRVEFLQTTQRNLLGEDLVPLSLKELEQLENQIEISLMNIRSSKNQQLLDQVFELKRKEQQLQDANKDLKRKIQETSGENMLHISCQDVGPSGHASEANQEFLHHAICDPSLHIGYQAYMDHLNQ*