OsMPK1;OsMAPK6

Gene Details

MSU gene ID: LOC_Os06g06090
RAPdb gene ID: Os06g0154500
Gene Symbol: OsMPK1 OsMAPK6 OsSIPK
Genome: MSU7 , IRGSP-1.0
Species: Oryza sativa

Functional Descriptions

Nipponbare) C(2) H(2) -type ZFP ZFP182 in ABA-induced antioxidant defense and the relationship between ZFP182 and two rice MAPKs, OsMPK1 and OsMPK5 in ABA signaling were investigated.
ABA treatment induced the increases in the expression of ZFP182, OsMPK1 and OsMPK5, and the activities of superoxide dismutase (SOD) and ascorbate peroxidase (APX) in rice leaves.
Besides, OsMPK1 and OsMPK5 were shown to be required for the up-regulation in the expression of ZFP182 in ABA signaling, but ZFP182 did not mediate the ABA-induced up-regulation in the expression of OsMPK1 and OsMPK5.
A time course (30-120 min) experiment using a variety of elicitors and stresses revealed that the OsSIPK mRNA is strongly induced by jasmonic acid (JA), salicylic acid (SA), ethephon, abscisic acid, cycloheximide (CHX), JA/SA + CHX, cantharidin, okadaic acid, hydrogen peroxide, chitosan, sodium chloride, and cold stress (12 degrees C), but not with wounding by cut, gaseous pollutants ozone, and sulfur dioxide, high temperature, ultraviolet C irradiation, sucrose, and drought.
ABA treatment induced the expression of OsDMI3 and OsMPK1 and the activities of OsDMI3 and OsMPK1 in rice leaves.
Our data indicate that there exists a cross-talk between OsDMI3 and OsMPK1 in ABA signalling, in which OsDMI3 functions upstream of OsMPK1 to regulate the activities of antioxidant enzymes and the production of H2 O2 in rice.
Of these, rice OsSIPK (Salicylic acid (SA)-Induced Protein Kinase) and its orthologs in other plants are of particular interest.
) mitogen-activated protein kinase (MAPK), OsSIPK (salicylic acid-induced protein kinase).
Steady state mRNA analyses of OsSIPK showed weak constitutive expression in leaves of 2-week-old rice seedlings.
In this minireview, we discuss the recent progress on rice OsSIPK and its orthologs as a central master switch for mediating plant responses against ozone, wounding, and JA as examples.
Involvement of the OsMKK4-OsMPK1 Cascade and its Downstream Transcription Factor OsWRKY53 in the Wounding Response in Rice.
Taken together, components involving in the wounding signaling pathway, OsMKK4-OsMPK1-OsWRKY53, can be important players in regulating crosstalk between abiotic stress and biotic stress.
OsMAPK6, a mitogen-activated protein kinase, influences rice grain size and biomass production.
Thus, OsMAPK6, a mitogen-activated protein kinase, plays a pivotal role in grain size, via cell proliferation, and BR signaling and homeostasis, in rice.
Further biochemical and genetic analyses reveal that OsMKKK10, OsMKK4 and OsMAPK6 function in a common pathway to control grain size.
Phosphorylation of bip130 by OsMPK1 regulates abscisic acid-induced antioxidant defense in rice.
Furthermore, bip130 can be phosphorylated by OsMPK1 at Thr-153 in聽vitro, and Thr-153 is essential for the ABA-induced antioxidant defense by OsMPK1.
These results reveal that OsMPK1 phosphorylates bip130 at Thr-153 to regulate ABA-induced antioxidant defense.
The OsMPK1:GFP and OsWRKY90:GFP transcription factor were localized to the nuclei, suggesting that OsMPK1 in the cytoplasm moves into the nuclei to interact with the WRKY90.
These combined results suggest that OsMEK2 and OsMPK1 expression positively regulates iron- and ROS-dependent ferroptotic cell death, and blast disease (susceptibility)-related cell death was ROS-dependent but iron-independent in rice-M.
Mitogen-Activated Protein Kinase OsMEK2 and OsMPK1 Signaling Is Required for Ferroptotic Cell Death in Rice- Magnaporthe oryzae Interactions.
The mitogen-activated protein kinase OsMPK1 is involved in abscisic acid (ABA) biosynthesis in rice (Oryza sativa L.
We also found that OsABA2 has feedback regulation on OsMPK1 kinase activity.
However, the underlying molecular mechanisms of OsMPK1 in regulating ABA biosynthesis are poorly understood.
Further research reveals that OsMPK1 and OsABA2 coordinately regulate the biosynthesis of ABA, and phosphorylation of OsABA2 at Ser197 by OsMPK1 plays a crucial role in regulating the biosynthesis of ABA.
Phosphorylation of OsABA2 at Ser197 by OsMPK1 regulates abscisic acid biosynthesis in rice.
Here, by using yeast two-hybrid assay and firefly luciferase complementary imaging assay, we show that OsMPK1 physically interact with a short-chain dehydrogenase protein OsABA2.
OsMAPK6 phosphorylates a zinc finger protein OsLIC to promote downstream OsWRKY30 for rice resistance to bacterial blight and leaf streak.
OsMAPK6 physically interacted with and phosphorylated OsLIC leading to decreased OsLIC DNA-binding activity, therefore, overexpression of OsLIC partially suppressed OsMAPK6-mediated rice resistance.

Functional Keywords

Literature and News

Gene Resources

Sequences

cDNA Sequence

>LOC_Os06g06090.1
ACACACAAAAAAAACACACACAAAAAAAAAAGAGGCGAGAGCTACAAAACGAGGCCGAAAGCGACCAAATCTCGCGACGAATTCCGCCTCCACTTTCCCTTCCCTTCCTCCTCCACCTCCACCTCCTCGTCGCGATCCAAATCCGAATCCGGCCATGGACGCCGGGGCGCAGCCGCCGGACACGGAGATGGCGGAGGCCGGCGGCGGGCAGCAGCCGCCTGCTGCGGCTGCGGCGGCGGGGGCGGGGGCAGGGGCGGGGATGATGGAGAACATCCAGGCGACGCTGAGCCATGGCGGGAGGTTCATCCAGTACAACATCTTCGGGAACGTGTTCGAGGTCACCGCCAAGTACAAGCCCCCCATCCTCCCCATCGGCAAGGGCGCCTACGGCATCGTCTGCTCGGCGCTCAACTCGGAGACGGGGGAGCAGGTGGCGATCAAGAAGATCGCCAACGCGTTCGACAACAAGATCGACGCCAAGCGCACGCTCAGGGAGATCAAGCTGCTCCGCCACATGGACCACGAGAATATTGTTGCCATAAGGGATATCATACCTCCTCCACAAAGGAATTCATTCAATGACGTTTATATTGCATATGAATTGATGGATACTGATCTGCATCAAATTATTCGCTCAAATCAAGCATTGTCAGAGGAGCACTGCCAGTATTTCCTTTATCAGATTCTCCGTGGCTTGAAGTATATACATTCAGCAAATGTCCTTCACCGAGACTTGAAGCCCAGCAACCTACTTTTGAATGCAAATTGTGACCTCAAAATTTGTGATTTTGGACTTGCTCGTACCACCTCAGAAACCGATTTTATGACTGAGTATGTTGTCACAAGATGGTATAGGGCACCGGAACTTCTGTTGAATTCCTCTGAATATACTGCAGCAATTGATGTGTGGTCTGTGGGCTGTATTTTTATGGAACTCATGGATCGTAAACCTTTGTTTCCTGGAAGAGATCATGTCCATCAATTACGTCTACTAATGGAGCTCATCGGAACGCCAAATGAGGCTGATCTGGATTTTGTAAATGAAAATGCAAGAAGATACATTCGCCAACTTCCTAGACATGCAAGGCAGTCCTTTCCTGAAAAATTTCCACATGTTCATCCTTTAGCAATTGATCTGGTTGAAAAGATGCTGACATTTGATCCTAGACAGAGAATAACAGTTGAAGGTGCCCTTGCACATCCTTACCTGGCATCACTGCATGACATAAGTGATGAGCCAGTCTGCTCATCACCCTTCAGCTTTGACTTCGAGCAGCATGCATTGTCCGAGGAACAAATGAAGGATCTAATCTACCAAGAAGGCCTTGCGTTCAACCCTGATTACCAGTAGCTGGTGTTCTATTTCAGCCTTGGATTGATTCTATTCATATGGAGTTTTTTCCTCCTGCGCCACAAAAGGTCGCCGACAGTGATCACTAGTTGTAAATAATTGCCTCACCTGAAAAATCCTCCCTGGTTCAAAGCTGAAGGTGTTGTTCTAAGAGTAGAAATGTACTTTGTGATCAAGTTCCTGGGTAGCTGCTATGCCATTCTTATGCTTATGTATGTTGTTTAATGTGGGATTTTTTTCCATCTTAAATGTTTTTAGTCCCTTTTGTAAGAAGAGTTAGTTCATGAACGATGACGGCCTAAATTCTGCGGTTATCATCAAATTCCCCATTTTCTTGTCGATTCATGGATTTCTCATGGTTTTACTTAATGCTCCATGTTGTAAGACGTGGTCAATGGAAGAGGATATATTGACTCTTGATTCAGTGGTGGCAGTTTGGAGTTGATCGTAAGACTGGAACATTA
>LOC_Os06g06090.2
ACACACAAAAAAAACACACACAAAAAAAAAAGAGGCGAGAGCTACAAAACGAGGCCGAAAGCGACCAAATCTCGCGACGAATTCCGCCTCCACTTTCCCTTCCCTTCCTCCTCCACCTCCACCTCCTCGTCGCGATCCAAATCCGAATCCGGCCATGGACGCCGGGGCGCAGCCGCCGGACACGGAGATGGCGGAGGCCGGCGGCGGGCAGCAGCCGCCTGCTGCGGCTGCGGCGGCGGGGGCGGGGGCAGGGGCGGGGATGATGGAGAACATCCAGGCGACGCTGAGCCATGGCGGGAGGTTCATCCAGTACAACATCTTCGGGAACGTGTTCGAGGTCACCGCCAAGTACAAGCCCCCCATCCTCCCCATCGGCAAGGGCGCCTACGGCATCGTCTGCTCGGCGCTCAACTCGGAGACGGGGGAGCAGGTGGCGATCAAGAAGATCGCCAACGCGTTCGACAACAAGATCGACGCCAAGCGCACGCTCAGGGAGATCAAGCTGCTCCGCCACATGGACCACGAGAATATTGTTGCCATAAGGGATATCATACCTCCTCCACAAAGGAATTCATTCAATGACGTTTATATTGCATATGAATTGATGGATACTGATCTGCATCAAATTATTCGCTCAAATCAAGCATTGTCAGAGGAGCACTGCCAGTATTTCCTTTATCAGATTCTCCGTGGCTTGAAGTATATACATTCAGCAAATGTCCTTCACCGAGACTTGAAGCCCAGCAACCTACTTTTGAATGCAAATTGTGACCTCAAAATTTGTGATTTTGGACTTGCTCGTACCACCTCAGAAACCGATTTTATGACTGAGTATGTTGTCACAAGATGGTATAGGGCACCGGAACTTCTGTTGAATTCCTCTGAATATACTGCAGCAATTGATGTGTGGTCTGTGGGCTGTATTTTTATGGAACTCATGGATCGTAAACCTTTGTTTCCTGGAAGAGATCATGTCCATCAATTACGTCTACTAATGGAGCTCATCGGAACGCCAAATGAGGCTGATCTGGATTTTGTAAATGAAAATGCAAGAAGATACATTCGCCAACTTCCTAGACATGCAAGGCAGTCCTTTCCTGAAAAATTTCCACATGTTCATCCTTTAGCAATTGATCTGGTTGAAAAGATGCTGACATTTGATCCTAGACAGAGAATAACAGTTGAAGGTGCCCTTGCACATCCTTACCTGGCATCACTGCATGACATAAGTGATGAGCCAGTCTGCTCATCACCCTTCAGCTTTGACTTCGAGCAGCATGCATTGTCCGAGGAACAAATGAAGGATCTAATCTACCAAGAAGGCCTTGCGTTCAACCCTGATTACCAGTAGCTGGTGTTCTATTTCAGCCTTGGATTGATTCTATTCATATGGAGTTTTTTCCTCCTGCGCCACAAAAGGTCGCCGACAGTGATCACTAGTTGTAAATAATTGCCTCACCTGAAAAATCCTCCCTGGTTCAAAGCTGAAGGTGTTGTTCTAAGAGTAGAAATGTACTTTGTGATCAAGTTCCTGGGTAGCTGCTATGCCATTCTTATGCTTATGTATGTTGTTTAATGTGGGATTTTTTTCCATCTTAAATGTTTTTAGTCCCTTTTGTAAGAAGAGTTAGTTCATGAACGATGACGGCCTAAATTCTGCGGTTATCATCAAATTCCCCATTTTCTTGTCGATTCATGGATTTCTCATGGTTTTACTTAATGCTCCATGTTGTAAGACGTGGTCAATGGAAGAGGATATATTGACTCTTGATTCAGTGGTGGCAGTTTGGAGTTGATCGTAAGACTGGAACATTA

CDS Sequence

>LOC_Os06g06090.1
ATGGACGCCGGGGCGCAGCCGCCGGACACGGAGATGGCGGAGGCCGGCGGCGGGCAGCAGCCGCCTGCTGCGGCTGCGGCGGCGGGGGCGGGGGCAGGGGCGGGGATGATGGAGAACATCCAGGCGACGCTGAGCCATGGCGGGAGGTTCATCCAGTACAACATCTTCGGGAACGTGTTCGAGGTCACCGCCAAGTACAAGCCCCCCATCCTCCCCATCGGCAAGGGCGCCTACGGCATCGTCTGCTCGGCGCTCAACTCGGAGACGGGGGAGCAGGTGGCGATCAAGAAGATCGCCAACGCGTTCGACAACAAGATCGACGCCAAGCGCACGCTCAGGGAGATCAAGCTGCTCCGCCACATGGACCACGAGAATATTGTTGCCATAAGGGATATCATACCTCCTCCACAAAGGAATTCATTCAATGACGTTTATATTGCATATGAATTGATGGATACTGATCTGCATCAAATTATTCGCTCAAATCAAGCATTGTCAGAGGAGCACTGCCAGTATTTCCTTTATCAGATTCTCCGTGGCTTGAAGTATATACATTCAGCAAATGTCCTTCACCGAGACTTGAAGCCCAGCAACCTACTTTTGAATGCAAATTGTGACCTCAAAATTTGTGATTTTGGACTTGCTCGTACCACCTCAGAAACCGATTTTATGACTGAGTATGTTGTCACAAGATGGTATAGGGCACCGGAACTTCTGTTGAATTCCTCTGAATATACTGCAGCAATTGATGTGTGGTCTGTGGGCTGTATTTTTATGGAACTCATGGATCGTAAACCTTTGTTTCCTGGAAGAGATCATGTCCATCAATTACGTCTACTAATGGAGCTCATCGGAACGCCAAATGAGGCTGATCTGGATTTTGTAAATGAAAATGCAAGAAGATACATTCGCCAACTTCCTAGACATGCAAGGCAGTCCTTTCCTGAAAAATTTCCACATGTTCATCCTTTAGCAATTGATCTGGTTGAAAAGATGCTGACATTTGATCCTAGACAGAGAATAACAGTTGAAGGTGCCCTTGCACATCCTTACCTGGCATCACTGCATGACATAAGTGATGAGCCAGTCTGCTCATCACCCTTCAGCTTTGACTTCGAGCAGCATGCATTGTCCGAGGAACAAATGAAGGATCTAATCTACCAAGAAGGCCTTGCGTTCAACCCTGATTACCAGTAG
>LOC_Os06g06090.2
ATGGACGCCGGGGCGCAGCCGCCGGACACGGAGATGGCGGAGGCCGGCGGCGGGCAGCAGCCGCCTGCTGCGGCTGCGGCGGCGGGGGCGGGGGCAGGGGCGGGGATGATGGAGAACATCCAGGCGACGCTGAGCCATGGCGGGAGGTTCATCCAGTACAACATCTTCGGGAACGTGTTCGAGGTCACCGCCAAGTACAAGCCCCCCATCCTCCCCATCGGCAAGGGCGCCTACGGCATCGTCTGCTCGGCGCTCAACTCGGAGACGGGGGAGCAGGTGGCGATCAAGAAGATCGCCAACGCGTTCGACAACAAGATCGACGCCAAGCGCACGCTCAGGGAGATCAAGCTGCTCCGCCACATGGACCACGAGAATATTGTTGCCATAAGGGATATCATACCTCCTCCACAAAGGAATTCATTCAATGACGTTTATATTGCATATGAATTGATGGATACTGATCTGCATCAAATTATTCGCTCAAATCAAGCATTGTCAGAGGAGCACTGCCAGTATTTCCTTTATCAGATTCTCCGTGGCTTGAAGTATATACATTCAGCAAATGTCCTTCACCGAGACTTGAAGCCCAGCAACCTACTTTTGAATGCAAATTGTGACCTCAAAATTTGTGATTTTGGACTTGCTCGTACCACCTCAGAAACCGATTTTATGACTGAGTATGTTGTCACAAGATGGTATAGGGCACCGGAACTTCTGTTGAATTCCTCTGAATATACTGCAGCAATTGATGTGTGGTCTGTGGGCTGTATTTTTATGGAACTCATGGATCGTAAACCTTTGTTTCCTGGAAGAGATCATGTCCATCAATTACGTCTACTAATGGAGCTCATCGGAACGCCAAATGAGGCTGATCTGGATTTTGTAAATGAAAATGCAAGAAGATACATTCGCCAACTTCCTAGACATGCAAGGCAGTCCTTTCCTGAAAAATTTCCACATGTTCATCCTTTAGCAATTGATCTGGTTGAAAAGATGCTGACATTTGATCCTAGACAGAGAATAACAGTTGAAGGTGCCCTTGCACATCCTTACCTGGCATCACTGCATGACATAAGTGATGAGCCAGTCTGCTCATCACCCTTCAGCTTTGACTTCGAGCAGCATGCATTGTCCGAGGAACAAATGAAGGATCTAATCTACCAAGAAGGCCTTGCGTTCAACCCTGATTACCAGTAG

Protein Sequence

>LOC_Os06g06090.1
MDAGAQPPDTEMAEAGGGQQPPAAAAAAGAGAGAGMMENIQATLSHGGRFIQYNIFGNVFEVTAKYKPPILPIGKGAYGIVCSALNSETGEQVAIKKIANAFDNKIDAKRTLREIKLLRHMDHENIVAIRDIIPPPQRNSFNDVYIAYELMDTDLHQIIRSNQALSEEHCQYFLYQILRGLKYIHSANVLHRDLKPSNLLLNANCDLKICDFGLARTTSETDFMTEYVVTRWYRAPELLLNSSEYTAAIDVWSVGCIFMELMDRKPLFPGRDHVHQLRLLMELIGTPNEADLDFVNENARRYIRQLPRHARQSFPEKFPHVHPLAIDLVEKMLTFDPRQRITVEGALAHPYLASLHDISDEPVCSSPFSFDFEQHALSEEQMKDLIYQEGLAFNPDYQ*
>LOC_Os06g06090.2
MDAGAQPPDTEMAEAGGGQQPPAAAAAAGAGAGAGMMENIQATLSHGGRFIQYNIFGNVFEVTAKYKPPILPIGKGAYGIVCSALNSETGEQVAIKKIANAFDNKIDAKRTLREIKLLRHMDHENIVAIRDIIPPPQRNSFNDVYIAYELMDTDLHQIIRSNQALSEEHCQYFLYQILRGLKYIHSANVLHRDLKPSNLLLNANCDLKICDFGLARTTSETDFMTEYVVTRWYRAPELLLNSSEYTAAIDVWSVGCIFMELMDRKPLFPGRDHVHQLRLLMELIGTPNEADLDFVNENARRYIRQLPRHARQSFPEKFPHVHPLAIDLVEKMLTFDPRQRITVEGALAHPYLASLHDISDEPVCSSPFSFDFEQHALSEEQMKDLIYQEGLAFNPDYQ*