Gene Details:

• MSU gene ID: LOC_Os06g06090
• RAPdb gene ID: Os06g0154500
• Gene Symbol: OsMPK1 OsMAPK6 OsSIPK
• Genome: MSU7 ,  IRGSP-1.0
• Species: Oryza sativa

Functional Descriptions:

• Nipponbare) C(2) H(2) -type ZFP ZFP182 in ABA-induced antioxidant defense and the relationship between ZFP182 and two rice MAPKs, OsMPK1 and OsMPK5 in ABA signaling were investigated.
• ABA treatment induced the increases in the expression of ZFP182, OsMPK1 and OsMPK5, and the activities of superoxide dismutase (SOD) and ascorbate peroxidase (APX) in rice leaves.
• Besides, OsMPK1 and OsMPK5 were shown to be required for the up-regulation in the expression of ZFP182 in ABA signaling, but ZFP182 did not mediate the ABA-induced up-regulation in the expression of OsMPK1 and OsMPK5.
• A time course (30-120 min) experiment using a variety of elicitors and stresses revealed that the OsSIPK mRNA is strongly induced by jasmonic acid (JA), salicylic acid (SA), ethephon, abscisic acid, cycloheximide (CHX), JA/SA + CHX, cantharidin, okadaic acid, hydrogen peroxide, chitosan, sodium chloride, and cold stress (12 degrees C), but not with wounding by cut, gaseous pollutants ozone, and sulfur dioxide, high temperature, ultraviolet C irradiation, sucrose, and drought.
• ABA treatment induced the expression of OsDMI3 and OsMPK1 and the activities of OsDMI3 and OsMPK1 in rice leaves.
• Our data indicate that there exists a cross-talk between OsDMI3 and OsMPK1 in ABA signalling, in which OsDMI3 functions upstream of OsMPK1 to regulate the activities of antioxidant enzymes and the production of H2 O2 in rice.
• Of these, rice OsSIPK (Salicylic acid (SA)-Induced Protein Kinase) and its orthologs in other plants are of particular interest.
• ) mitogen-activated protein kinase (MAPK), OsSIPK (salicylic acid-induced protein kinase).
• Steady state mRNA analyses of OsSIPK showed weak constitutive expression in leaves of 2-week-old rice seedlings.
• In this minireview, we discuss the recent progress on rice OsSIPK and its orthologs as a central master switch for mediating plant responses against ozone, wounding, and JA as examples.
• Involvement of the OsMKK4-OsMPK1 Cascade and its Downstream Transcription Factor OsWRKY53 in the Wounding Response in Rice.
• Taken together, components involving in the wounding signaling pathway, OsMKK4-OsMPK1-OsWRKY53, can be important players in regulating crosstalk between abiotic stress and biotic stress.
• OsMAPK6, a mitogen-activated protein kinase, influences rice grain size and biomass production.
• Thus, OsMAPK6, a mitogen-activated protein kinase, plays a pivotal role in grain size, via cell proliferation, and BR signaling and homeostasis, in rice.
• Further biochemical and genetic analyses reveal that OsMKKK10, OsMKK4 and OsMAPK6 function in a common pathway to control grain size.
• Phosphorylation of bip130 by OsMPK1 regulates abscisic acid-induced antioxidant defense in rice.
• Furthermore, bip130 can be phosphorylated by OsMPK1 at Thr-153 in聽vitro, and Thr-153 is essential for the ABA-induced antioxidant defense by OsMPK1.
• These results reveal that OsMPK1 phosphorylates bip130 at Thr-153 to regulate ABA-induced antioxidant defense.
• The OsMPK1:GFP and OsWRKY90:GFP transcription factor were localized to the nuclei, suggesting that OsMPK1 in the cytoplasm moves into the nuclei to interact with the WRKY90.
• These combined results suggest that OsMEK2 and OsMPK1 expression positively regulates iron- and ROS-dependent ferroptotic cell death, and blast disease (susceptibility)-related cell death was ROS-dependent but iron-independent in rice-M.
• Mitogen-Activated Protein Kinase OsMEK2 and OsMPK1 Signaling Is Required for Ferroptotic Cell Death in Rice- Magnaporthe oryzae Interactions.
• The mitogen-activated protein kinase OsMPK1 is involved in abscisic acid (ABA) biosynthesis in rice (Oryza sativa L.
• We also found that OsABA2 has feedback regulation on OsMPK1 kinase activity.
• However, the underlying molecular mechanisms of OsMPK1 in regulating ABA biosynthesis are poorly understood.
• Further research reveals that OsMPK1 and OsABA2 coordinately regulate the biosynthesis of ABA, and phosphorylation of OsABA2 at Ser197 by OsMPK1 plays a crucial role in regulating the biosynthesis of ABA.
• Phosphorylation of OsABA2 at Ser197 by OsMPK1 regulates abscisic acid biosynthesis in rice.
• Here, by using yeast two-hybrid assay and firefly luciferase complementary imaging assay, we show that OsMPK1 physically interact with a short-chain dehydrogenase protein OsABA2.
• OsMAPK6 phosphorylates a zinc finger protein OsLIC to promote downstream OsWRKY30 for rice resistance to bacterial blight and leaf streak.
• OsMAPK6 physically interacted with and phosphorylated OsLIC leading to decreased OsLIC DNA-binding activity, therefore, overexpression of OsLIC partially suppressed OsMAPK6-mediated rice resistance.

Function-related keywords:

• ABA ,  defense ,  cold-stress ,  jasmonic-acid ,  jasmonic ,  salicylic-acid ,  temperature ,  drought ,  seedling ,  ja ,  wounding-response ,  abiotic-stress ,  biotic-stress ,  grain-size ,  protein-kinase ,  biomass-production ,  biomass ,  cell-proliferation ,  BR-signaling ,  BR-homeostasis ,  grain ,  abscisic-acid ,  transcription-factor ,  disease ,  blast ,  magnaporthe-oryzae ,  cell-death ,  cytoplasm ,  Kinase ,  kinase ,  blast-disease ,  ABA-biosynthesis ,  dehydrogenase ,  abscisic-acid-biosynthesis ,  leaf ,  resistance ,  blight ,  bacterial-blight ,  R-protein ,  zinc

Literature:

• Novel rice OsSIPK is a multiple stress responsive MAPK family member showing rhythmic expression at mRNA level .  DOI:  10.1007/s00425-007-0672-2 ;  PMID:  18066586
• The C2H2-type zinc finger protein ZFP182 is involved in abscisic acid-induced antioxidant defense in rice .  DOI:  10.1111/j.1744-7909.2012.01135.x ;  PMID:  22693960
• The bHLH Rac Immunity1 (RAI1) Is Activated by OsRac1 via OsMAPK3 and OsMAPK6 in Rice Immunity .  DOI:  10.1093/pcp/pcs033 ;  PMID:  22437844
• Molecular analysis of the rice MAP kinase gene family in relation to Magnaporthe grisea infection .  DOI:  10.1094/MPMI-19-0530 ;  PMID:  16673940
• OsDMI3-mediated activation of OsMPK1 regulates the activities of antioxidant enzymes in abscisic acid signalling in rice .  DOI:  10.1111/pce.12154 ;  PMID:  23777258
• Involvement of the OsMKK4-OsMPK1 Cascade and its Downstream Transcription Factor OsWRKY53 in the Wounding Response in Rice .  DOI:  10.5423/PPJ.OA.10.2013.0106 ;  PMID:  25288999
• OsMAPK6, a mitogen-activated protein kinase, influences rice grain size and biomass production .  DOI:  10.1111/tpj.13025 ;  PMID:  26366992
• Control of Grain Size and Weight by the OsMKKK10-OsMKK4-OsMAPK6 Signaling Pathway in Rice .  DOI:  10.1016/j.molp.2018.04.004 ;  PMID:  29702261
• Differential Responses of OsMPK s in IR56 Rice to Two BPH Populations of Different Virulence Levels .  DOI:  10.3390/ijms19124030 ;  PMID:  30551584
• Phosphorylation of bip130 by OsMPK1 regulates abscisic acid-induced antioxidant defense in rice .  DOI:  10.1016/j.bbrc.2019.04.183 ;  PMID:  31078272
• OrMKK3 Influences Morphology and Grain Size in Rice .  DOI:  10.1007/s12374-020-09290-2 ;  PMID:  33424241
• Mitogen-Activated Protein Kinase OsMEK2 and OsMPK1 Signaling Is Required for Ferroptotic Cell Death in Rice- Magnaporthe oryzae Interactions .  DOI:  10.3389/fpls.2021.710794 ;  PMID:  34408766
• Phosphorylation of OsABA2 at Ser197 by OsMPK1 regulates abscisic acid biosynthesis in rice .  DOI:  10.1016/j.bbrc.2021.11.067 ;  PMID:  34826703
• OsMAPK6 phosphorylates a zinc finger protein OsLIC to promote downstream OsWRKY30 for rice resistance to bacterial blight and leaf streak .  DOI:  10.1111/jipb.13249 ;  PMID:  35293133
• Rice iron storage protein ferritin 2 (OsFER2) positively regulates ferroptotic cell death and defense responses against Magnaporthe oryzae .  DOI:  10.3389/fpls.2022.1019669 ;  PMID:  36352872
• NA .

Related News:

Gene Resources:

• NCBI ID: AB183398  , AJ535841
• UniProt accessions:

Sequences: