Information report for OsMKK1;OsMEK2
Gene Details
|
|
Functional Descriptions
- By comparing the transcription factors levels between WT and OsMKK1 mutant, OsMKK1 was necessary for salt-induced increase in OsDREB2B and OsMYBS3.
- OsMKK1 activity in the wild-type seedlings and protoplasts was increased by salt stress.
- Taken together, the data suggest that OsMKK1 and OsMPK4 constitute a signaling pathway that regulates salt stress tolerance in rice.
- Yeast two-hybrid and in vitro and in vivo kinase assays revealed that OsMKK1 targeted OsMPK4.
- Further analyses revealed that OsDMI3-mediated phosphorylation of OsMKK1 positively regulates ABA responses in seed germination, root growth, and tolerance to both water stress and oxidative stress.
- The activation of OsMKK1 and its downstream kinase OsMPK1 is dependent on Thr-25 phosphorylation of OsMKK1 in ABA signaling.
- Our results indicate that OsMKK1 is a direct target of OsDMI3, and OsDMI3-mediated phosphorylation of OsMKK1 plays an important role in activating the MAPK cascade and ABA signaling.
- These combined results suggest that OsMEK2 and OsMPK1 expression positively regulates iron- and ROS-dependent ferroptotic cell death, and blast disease (susceptibility)-related cell death was ROS-dependent but iron-independent in rice-M.
- Mitogen-Activated Protein Kinase OsMEK2 and OsMPK1 Signaling Is Required for Ferroptotic Cell Death in Rice- Magnaporthe oryzae Interactions.
- OsFER2 knock-out in wild-type rice HY did not induce ROS and ferric ion (Fe(3+)) accumulation, lipid peroxidation and hypersensitive response (HR) cell death, and also downregulated the defense-related genes OsPAL1, OsPR1-b, OsRbohB, OsNADP-ME2-3, OsMEK2 and OsMPK1, and vacuolar membrane transporter OsVIT2 expression.
Functional Keywords
- transcription-factor , salt , salt-stress , tolerance , stress , stress-tolerance , Kinase , seedlings , root , seed , oxidative-stress , seed-germination , oxidative , ABA , kinase , root-growth , ER-stress , disease , blast , magnaporthe-oryzae , cell-death , protein-kinase , blast-disease , transporter
Literature and News
- The mitogen-activated protein kinase cascade MKK1-MPK4 mediates salt signaling in rice . DOI: 10.1016/j.plantsci.2014.08.007 ; PMID: 25219319
- Rice calcium/calmodulin-dependent protein kinase directly phosphorylates a mitogen-activated protein kinase kinase to regulate abscisic acid responses . DOI: 10.1093/plcell/koab071 ; PMID: 33630095
- Mitogen-Activated Protein Kinase OsMEK2 and OsMPK1 Signaling Is Required for Ferroptotic Cell Death in Rice- Magnaporthe oryzae Interactions . DOI: 10.3389/fpls.2021.710794 ; PMID: 34408766
- Rice iron storage protein ferritin 2 (OsFER2) positively regulates ferroptotic cell death and defense responses against Magnaporthe oryzae . DOI: 10.3389/fpls.2022.1019669 ; PMID: 36352872
Gene Resources
- UniProt: Q5VP37
- EMBL: AK111598, AP014962
- AlphaFoldDB: Q5VP37
- EnsemblPlants: Os06t0147800-01
- Gramene: Os06t0147800-01
- KEGG: osa:4340126
- Orthologous matrix: QMTLTEP
- InterPro: IPR000719, IPR001245, IPR008271
- PANTHER: PTHR48013, PTHR48013:SF32
- SUPFAM: SSF56112
- PROSITE: PS00107, PS00108, PS50011
- Gene3D: 1.10.510.10, 3.30.200.20
- OrthoDB: Q5VP37
- SWISS-MODEL: Q5VP37
- Conserved Domain Database: cd06623
- eggNOG: KOG0581
Sequences
cDNA Sequence
- >LOC_Os06g05520.1
ATGGTGTTCGACTCGTCTCCATTTCGCGTCTCCTCCTCCTCCTCCTCCGGCCATCTCACCCTCCCGGCGAGCAGCGAGCGGGAGGCCTCCGCCACCGCCGCCGCCGCCGCCGCCGTCGCCGCCCGATGGGGAAGCCGGGGAAGCTAGCGCTGCCCTCGCACGAGTCAACCATCGGCAAATTCCTGACGCAGAGCGGGACGTTCAAGGACGGCGATCTGCTCGTCAACAAGGACGGCCTCCGCATCGTCTCGCAGAGCGAGGAAGGGGAGGCCCCTCCTATAGAACCTTTAGATCATAATCAGTTGAGTCTAGATGATCTAGACGCAATCAAAGTTATCGGTAAAGGTAGTAGTGGAATCGTGCAGTTGGTTCGCCACAAATGGACTGGCCAGTTTTTTGCTCTGAAGGTAATACAACTCAATATCCAGGAGAATATACGCAGACAGATTGCGCAGGAATTGAAAATCAGCTTGTCAACACAGTGCCAATATGTTGTTGCATGCTGTCAGTGTTTTTACGTTAATGGTGTCATTTCAATTGTTTTGGAGTATATGGACAGTGGCTCTCTCTCAGATTTCCTGAAGACAGTTAAAACCATTCCAGAGCCCTACCTTGCTGCAATCTGTAAGCAGGTGCTAAAAGGACTGATGTACTTACATCATGAGAAGCACATCATACACCGAGATCTGAAGCCGTCAAATATATTAATAAATCATATGGGTGAAGTAAAAATATCCGACTTTGGTGTTAGTGCCATCATTGCTAGTTCCTCGGCACAACGAGATACATTTACTGGGACATATAACTACATGGCGCCAGAAAGAATCAGTGGGCAAAAACATGGTTATATGAGTGATATCTGGAGCTTGGGCCTCGTTATGCTAGAATTGGCCACTGGTGAATTTCCATATCCTCCTCGTGAAAGCTTTTATGAACTCCTTGAAGCTGTTGTCGACCACCCACCACCTTCTGCTCCATCAGACCAGTTTTCAGAGGAATTCTGTTCATTCGTCTCTGCATGTATCCAAAAGAATGCTTCGGATAGGTCATCTGCACAAATCTTATTAAATCATCCATTCCTGAGCATGTATGACGACCTGAATATAGATCTTGCTTCGTACTTCACAACCGATGGATCTCCGCTTGCCACCTTCAATACAAGCAACCGATATGATGACAGATAGTCTGATGCAACACTGCGTCATCATCTAGCTCGAAGGATGATCCTTTGGCTGTCCATCTGTAGAATCTACCATCTACCTAACTGGCCCCAAATGTTTCGTGTATTTTTTGGGAGAAAACTTTGCCCTGTGTACTGAGCTGGGATTGATGTCTTTGATATGTAAATTTCGCGTAATTTCATATGTAGAACAATTCTAGGATCATTGGGCATTGAAGTTTTGTTTGGCCGTGCACCAGGATGATCTGTTCTTGATCGCTTTACCTTTGTTTCTCACTTCTGTAACTAAAACCTATATTTACCATTTACCGGATTGTTCATGGCCTCTTCTGGGCAAGTCGGTGTCGACTGAAAGTCTCAAACAATTGCCTCTTC
CDS Sequence
- >LOC_Os06g05520.1
ATGGGGAAGCCGGGGAAGCTAGCGCTGCCCTCGCACGAGTCAACCATCGGCAAATTCCTGACGCAGAGCGGGACGTTCAAGGACGGCGATCTGCTCGTCAACAAGGACGGCCTCCGCATCGTCTCGCAGAGCGAGGAAGGGGAGGCCCCTCCTATAGAACCTTTAGATCATAATCAGTTGAGTCTAGATGATCTAGACGCAATCAAAGTTATCGGTAAAGGTAGTAGTGGAATCGTGCAGTTGGTTCGCCACAAATGGACTGGCCAGTTTTTTGCTCTGAAGGTAATACAACTCAATATCCAGGAGAATATACGCAGACAGATTGCGCAGGAATTGAAAATCAGCTTGTCAACACAGTGCCAATATGTTGTTGCATGCTGTCAGTGTTTTTACGTTAATGGTGTCATTTCAATTGTTTTGGAGTATATGGACAGTGGCTCTCTCTCAGATTTCCTGAAGACAGTTAAAACCATTCCAGAGCCCTACCTTGCTGCAATCTGTAAGCAGGTGCTAAAAGGACTGATGTACTTACATCATGAGAAGCACATCATACACCGAGATCTGAAGCCGTCAAATATATTAATAAATCATATGGGTGAAGTAAAAATATCCGACTTTGGTGTTAGTGCCATCATTGCTAGTTCCTCGGCACAACGAGATACATTTACTGGGACATATAACTACATGGCGCCAGAAAGAATCAGTGGGCAAAAACATGGTTATATGAGTGATATCTGGAGCTTGGGCCTCGTTATGCTAGAATTGGCCACTGGTGAATTTCCATATCCTCCTCGTGAAAGCTTTTATGAACTCCTTGAAGCTGTTGTCGACCACCCACCACCTTCTGCTCCATCAGACCAGTTTTCAGAGGAATTCTGTTCATTCGTCTCTGCATGTATCCAAAAGAATGCTTCGGATAGGTCATCTGCACAAATCTTATTAAATCATCCATTCCTGAGCATGTATGACGACCTGAATATAGATCTTGCTTCGTACTTCACAACCGATGGATCTCCGCTTGCCACCTTCAATACAAGCAACCGATATGATGACAGATAG
Protein Sequence
- >LOC_Os06g05520.1
MGKPGKLALPSHESTIGKFLTQSGTFKDGDLLVNKDGLRIVSQSEEGEAPPIEPLDHNQLSLDDLDAIKVIGKGSSGIVQLVRHKWTGQFFALKVIQLNIQENIRRQIAQELKISLSTQCQYVVACCQCFYVNGVISIVLEYMDSGSLSDFLKTVKTIPEPYLAAICKQVLKGLMYLHHEKHIIHRDLKPSNILINHMGEVKISDFGVSAIIASSSAQRDTFTGTYNYMAPERISGQKHGYMSDIWSLGLVMLELATGEFPYPPRESFYELLEAVVDHPPPSAPSDQFSEEFCSFVSACIQKNASDRSSAQILLNHPFLSMYDDLNIDLASYFTTDGSPLATFNTSNRYDDR*