Gene Details:

• MSU gene ID: LOC_Os06g04280
• RAPdb gene ID: Os06g0133900
• Gene Symbol: EPSPs OsEPSPS
• Genome: MSU7 ,  IRGSP-1.0
• Species: Oryza sativa

Functional Descriptions:

• Here, we describe the development of glyphosate-resistant rice lines by site-specific amino acid substitutions (G172A, T173I, and P177S: GATIPS-mOsEPSPS) and modification of phosphoenolpyruvate-binding site in the native OsEPSPS gene employing fragment knockout and knock-in of homology donor repair (HDR) template harboring desired mutations through CRISPR-Cas9-based genome editing.
• Together, results highlighted that the efficacy of GATIPS mutations in OsEPSPS has tremendously contributed in glyphosate resistance (foliar spray of 6 ml/L), enhanced aromatic amino acids, and improved grain yields in rice.

Function-related keywords:

• development ,  grain ,  resistance ,  grain-yield

Literature:

• Evidence for an evolutionary force that prevents epigenetic silencing between tail-to-tail rice genes with a short spacer .  DOI:  10.1016/j.gene.2004.11.012 ;  PMID:  15716045
• Gene replacements and insertions in rice by intron targeting using CRISPR-Cas9 .  DOI:  10.1038/nplants.2016.139 ;  PMID:  27618611
• Overexpression of improved EPSPS gene results in field level glyphosate tolerance and higher grain yield in rice .  DOI:  10.1111/pbi.13428 ;  PMID:  32516520
• CRISPR/Cas9-mediated homology donor repair base editing confers glyphosate resistance to rice (Oryza sativa L.) .  DOI:  10.3389/fpls.2023.1122926 ;  PMID:  36959937

Related News:

Gene Resources:

• NCBI ID: AB052962
• UniProt accessions:

Sequences: