Gene Details:

Functional Descriptions:

  • The OsBBI1-overexpressing plants showed higher levels of H(2)O(2) accumulation in cells and higher levels of phenolic compounds and cross-linking of proteins in cell walls at infection sites by M.
  • The cell walls were thicker in the OsBBI1-overexpressing plants and thinner in the mutant plants than in the WT plants.
  • Our results suggest that OsBBI1 modulates broad-spectrum resistance to blast fungus by modifying cell wall defence responses.
  • Rice RING protein OsBBI1 with E3 ligase activity confers broad-spectrum resistance against Magnaporthe oryzae by modifying the cell wall defence.
  • The expression of OsBBI1 was induced by rice blast fungus Magnaporthe oryzae, as well as chemical inducers, benzothiadiazole and salicylic acid.
  • This indicates that OsBBI1 modulates broad-spectrum resistance against the blast fungus.
  • We report that a rice gene, OsBBI1, encoding a RING finger protein with E3 ligase activity, mediates broad-spectrum disease resistance.
  • The functional characterization of OsBBI1 provides insight into the E3 ligase-mediated innate immunity, and a practical tool for constructing broad-spectrum resistance against the most destructive disease in rice.

Literature:

Gene Resources:

  • NCBI ID:
  • UniProt accessions:

Sequences:

cDNA Sequence
  • >LOC_Os06g03580.3
    ATTAACAAAGAATCCGTCCAGCCTTGAGAAGCAGAGAAGAGGAGAGAAACAAGAGACAGACAGACAGAGATAGAGTAACTGAGTAAACGGGTTCTTGCCTGACATTTGTCTTGTCCGGGTGGCCATTCATGGCCACCGTGGGGCAGCCTGATGCCATGAGAAGGATCACCGTTCACTACGTGAATCCGCCGCCGATCGCCGGCGCCGGAGAAGCACACGTCGATGGCCTCGACGACGAGGTTCTTGATTATGTGATTGGTGATGTTCTTCAGGATCAGGAGGGTCTGTATCAATCGATATTGTATGGCAAATACGGGGATGACATGAGAGGGGCAAGAAACACTGCTCTTGCCCAATCTGATGGTTTACATTACTACTATCACGGAGAGAACAGCAGTGGTGAAGCAACAACCTCGAGAAATTCAGAAATCGACCAACAGATTGAGTATGACTTGGTGTTCGCTAGGCAGCTGCAGGCAATGGATAATCTGACAATTGAGACACCTGCAGATGAAGATGATGATATAAGCTGCGTACCCTCTCCATCTGACTCTGAAACCGATGAACCGGCAGAAGGCAACAACGAAGAGGCTGCCACACAGGATGATAACGATGATCCAGACAACATGACATACGAGCAAAGGCAGGCACTTGTGGAATCTGTAGGAAATGAGAACAGAGGTTTATCTGATCTGCTTATCTCCTACTTGGAGACATGGAAGTACAAATCAGGCTTTTTCCCAAGGAAGGCAAACCATGATAACTGTCCTATATGTCTATCCGCTTTCAGACGCCGAGAAACACTCATTACATTGGCTTGCAAGCATAGTTACCATGAAGGTTGTATTGCTAGATGGCTCAAGATTGACAAGGTAAACACCTTCTTGGGACACTAGATTTGCTATCTGAATTGTGTACCTATCTTAGATTATACCATTTGTCAAAGTTTACATGAGCACTGATATGCACTGTCCATTTCATATAACAAGATGGTCTTTGTCTTTCAGTTTCCTTGAGCTTCTTCCAAATTAAAAGAACTTCATGGGCTTGTGCAGTGGAATTCCTGACATTTTTGTTTATGAAACAGGCCTGCCCAGTTTGCAAATATGAAGTGTTTGGACCTTCCTAGCGTGTGAGGAGGCGATCAACAAGGCTGACCAACTATGACTCGATGAGACGGCATATAGTGGTTGCAGCATCTGCTCTGCAGTATTAATTAAAAAGGGAAACCTTAACTCCCAAAAAAAACAGAGAAGGGAAGAAAAAGAAAGCAAAAAGGAAAAAGGAAAAAGAAAAAGAAAAGACAAGGAGAAAAGAAATTTGAAAACAGAGATGCTTCGTCAAGATTGTGCATTTTTTTCATGTTTTTTTTTGGCTGTGTACAAGCGTAATTTTGACCTGATCGACTCAAGGCAGTCGCTCGAGGCCTTTAATTTCGTAAAGCTTCATATGCATGAGTGTGCAATTGTGTGCCTATAGTAGTATAGTACATGTTTTCTGTTTGAAACGGAGTCAAAGATCTCTCTCCTGCCGACCACCCATCTAAAAGAAGGCCATGTGTACATCAATACATGTACTGATATATACTCCCTCTGTCCAAAATAAATCAACTTTTGTACATGTCAA
  • >LOC_Os06g03580.1
    ATTAACAAAGAATCCGTCCAGCCTTGAGAAGCAGAGAAGAGGAGAGAAACAAGAGACAGACAGACAGAGATAGAGTAACTGAGTAAACGGGTTCTTGCCTGACATTTGTCTTGTCCGGGTGGCCATTCATGGCCACCGTGGGGCAGCCTGATGCCATGAGAAGGATCACCGTTCACTACGTGAATCCGCCGCCGATCGCCGGCGCCGGAGAAGCACACGTCGATGGCCTCGACGACGAGGTTCTTGATTATGTGATTGGTGATGTTCTTCAGGATCAGGAGGGTCTGTATCAATCGATATTGTATGGCAAATACGGGGATGACATGAGAGGGGCAAGAAACACTGCTCTTGCCCAATCTGATGGTTTACATTACTACTATCACGGAGAGAACAGCAGTGGTGAAGCAACAACCTCGAGAAATTCAGAAATCGACCAACAGATTGAGTATGACTTGGTGTTCGCTAGGCAGCTGCAGGCAATGGATAATCTGACAATTGAGACACCTGCAGATGAAGATGATGATATAAGCTGCGTACCCTCTCCATCTGACTCTGAAACCGATGAACCGGCAGAAGGCAACAACGAAGAGGCTGCCACACAGGATGATAACGATGATCCAGACAACATGACATACGAGCAAAGGCAGGCACTTGTGGAATCTGTAGGAAATGAGAACAGAGGTTTATCTGATCTGCTTATCTCCTACTTGGAGACATGGAAGTACAAATCAGGCTTTTTCCCAAGGAAGGCAAACCATGATAACTGTCCTATATGTCTATCCGCTTTCAGACGCCGAGAAACACTCATTACATTGGCTTGCAAGCATAGTTACCATGAAGGTTGTATTGCTAGATGGCTCAAGATTGACAAGGTAAACACCTTCTTGGGACACTAGATTTGCTATCTGAATTGTGTACCTATCTTAGATTATACCATTTGTCAAAGTTTACATGAGCACTGATATGCACTGTCCATTTCATATAACAAGATGGTCTTTGTCTTTCAGTTTCCTTGAGCTTCTTCCAAATTAAAAGAACTTCATGGGCTTGTGCAGTGGAATTCCTGACATTTTTGTTTATGAAACAGGCCTGCCCAGTTTGCAAATATGAAGTGTTTGGACCTTCCTAGCGTGTGAGGAGGCGATCAACAAGGCTGACCAACTATGACTCGATGAGACGGCATATAGTGGTTGCAGCATCTGCTCTGCAGTATTAATTAAAAAGGGAAACCTTAACTCCCAAAAAAAACAGAGAAGGGAAGAAAAAGAAAGCAAAAAGGAAAAAGGAAAAAGAAAAAGAAAAGACAAGGAGAAAAGAAATTTGAAAACAGAGATGCTTCGTCAAGATTGTGCATTTTTTTCATGTTTTTTTTTGGCTGTGTACAAGCGTAATTTTGACCTGATCGACTCAAGGCAGTCGCTCGAGGCCTTTAATTTCGTAAAGCTTCATATGCATGAGTGTGCAATTGTGTGCCTATAGTAGTATAGTACATGTTTTCTGTTTGAAACGGAGTCAAAGATCTCTCTCCTGCCGACCACCCATCTAAAAGAAGGCCATGTGTACATCAATACATGTACTGATATATACTCCCTCTGTCCAAAATAAATCAACTTTTGTACATGTCAA
  • >LOC_Os06g03580.4
    ATTAACAAAGAATCCGTCCAGCCTTGAGAAGCAGAGAAGAGGAGAGAAACAAGAGACAGACAGACAGAGATAGAGTAACTGAGTAAACGGGTTCTTGCCTGACATTTGTCTTGTCCGGGTGGCCATTCATGGCCACCGTGGGGCAGCCTGATGCCATGAGAAGGATCACCGTTCACTACGTGAATCCGCCGCCGATCGCCGGCGCCGGAGAAGCACACGTCGATGGCCTCGACGACGAGGTTCTTGATTATGTGATTGGTGATGTTCTTCAGGATCAGGAGGGTCTGTATCAATCGATATTGTATGGCAAATACGGGGATGACATGAGAGGGGCAAGAAACACTGCTCTTGCCCAATCTGATGGTTTACATTACTACTATCACGGAGAGAACAGCAGTGGTGAAGCAACAACCTCGAGAAATTCAGAAATCGACCAACAGATTGAGTATGACTTGGTGTTCGCTAGGCAGCTGCAGGCAATGGATAATCTGACAATTGAGACACCTGCAGATGAAGATGATGATATAAGCTGCGTACCCTCTCCATCTGACTCTGAAACCGATGAACCGGCAGAAGGCAACAACGAAGAGGCTGCCACACAGGATGATAACGATGATCCAGACAACATGACATACGAGCAAAGGCAGGCACTTGTGGAATCTGTAGGAAATGAGAACAGAGGTTTATCTGATCTGCTTATCTCCTACTTGGAGACATGGAAGTACAAATCAGGCTTTTTCCCAAGGAAGGCAAACCATGATAACTGTCCTATATGTCTATCCGCTTTCAGACGCCGAGAAACACTCATTACATTGGCTTGCAAGCATAGTTACCATGAAGGTTGTATTGCTAGATGGCTCAAGATTGACAAGGTAAACACCTTCTTGGGACACTAGATTTGCTATCTGAATTGTGTACCTATCTTAGATTATACCATTTGTCAAAGTTTACATGAGCACTGATATGCACTGTCCATTTCATATAACAAGATGGTCTTTGTCTTTCAGTTTCCTTGAGCTTCTTCCAAATTAAAAGAACTTCATGGGCTTGTGCAGTGGAATTCCTGACATTTTTGTTTATGAAACAGGCCTGCCCAGTTTGCAAATATGAAGTGTTTGGACCTTCCTAGCGTGTGAGGAGGCGATCAACAAGGCTGACCAACTATGACTCGATGAGACGGCATATAGTGGTTGCAGCATCTGCTCTGCAGTATTAATTAAAAAGGGAAACCTTAACTCCCAAAAAAAACAGAGAAGGGAAGAAAAAGAAAGCAAAAAGGAAAAAGGAAAAAGAAAAAGAAAAGACAAGGAGAAAAGAAATTTGAAAACAGAGATGCTTCGTCAAGATTGTGCATTTTTTTCATGTTTTTTTTTGGCTGTGTACAAGCGTAATTTTGACCTGATCGACTCAAGGCAGTCGCTCGAGGCCTTTAATTTCGTAAAGCTTCATATGCATGAGTGTGCAATTGTGTGCCTATAGTAGTATAGTACATGTTTTCTGTTTGAAACGGAGTCAAAGATCTCTCTCCTGCCGACCACCCATCTAAAAGAAGGCCATGTGTACATCAATACATGTACTGATATATACTCCCTCTGTCCAAAATAAATCAACTTTTGTACATGTCAA
  • >LOC_Os06g03580.2
    ATTAACAAAGAATCCGTCCAGCCTTGAGAAGCAGAGAAGAGGAGAGAAACAAGAGACAGACAGACAGAGATAGAGTAACTGAGTAAACGGGTTCTTGCCTGACATTTGTCTTGTCCGGGTGGCCATTCATGGCCACCGTGGGGCAGCCTGATGCCATGAGAAGGATCACCGTTCACTACGTGAATCCGCCGCCGATCGCCGGCGCCGGAGAAGCACACGTCGATGGCCTCGACGACGAGGTTCTTGATTATGTGATTGGTGATGTTCTTCAGGATCAGGAGGGTCTGTATCAATCGATATTGTATGGCAAATACGGGGATGACATGAGAGGGGCAAGAAACACTGCTCTTGCCCAATCTGATGGTTTACATTACTACTATCACGGAGAGAACAGCAGTGGTGAAGCAACAACCTCGAGAAATTCAGAAATCGACCAACAGATTGAGTATGACTTGGTGTTCGCTAGGCAGCTGCAGGCAATGGATAATCTGACAATTGAGACACCTGCAGATGAAGATGATGATATAAGCTGCGTACCCTCTCCATCTGACTCTGAAACCGATGAACCGGCAGAAGGCAACAACGAAGAGGCTGCCACACAGGATGATAACGATGATCCAGACAACATGACATACGAGCAAAGGCAGGCACTTGTGGAATCTGTAGGAAATGAGAACAGAGGTTTATCTGATCTGCTTATCTCCTACTTGGAGACATGGAAGTACAAATCAGGCTTTTTCCCAAGGAAGGCAAACCATGATAACTGTCCTATATGTCTATCCGCTTTCAGACGCCGAGAAACACTCATTACATTGGCTTGCAAGCATAGTTACCATGAAGGTTGTATTGCTAGATGGCTCAAGATTGACAAGGTAAACACCTTCTTGGGACACTAGATTTGCTATCTGAATTGTGTACCTATCTTAGATTATACCATTTGTCAAAGTTTACATGAGCACTGATATGCACTGTCCATTTCATATAACAAGATGGTCTTTGTCTTTCAGTTTCCTTGAGCTTCTTCCAAATTAAAAGAACTTCATGGGCTTGTGCAGTGGAATTCCTGACATTTTTGTTTATGAAACAGGCCTGCCCAGTTTGCAAATATGAAGTGTTTGGACCTTCCTAGCGTGTGAGGAGGCGATCAACAAGGCTGACCAACTATGACTCGATGAGACGGCATATAGTGGTTGCAGCATCTGCTCTGCAGTATTAATTAAAAAGGGAAACCTTAACTCCCAAAAAAAACAGAGAAGGGAAGAAAAAGAAAGCAAAAAGGAAAAAGGAAAAAGAAAAAGAAAAGACAAGGAGAAAAGAAATTTGAAAACAGAGATGCTTCGTCAAGATTGTGCATTTTTTTCATGTTTTTTTTTGGCTGTGTACAAGCGTAATTTTGACCTGATCGACTCAAGGCAGTCGCTCGAGGCCTTTAATTTCGTAAAGCTTCATATGCATGAGTGTGCAATTGTGTGCCTATAGTAGTATAGTACATGTTTTCTGTTTGAAACGGAGTCAAAGATCTCTCTCCTGCCGACCACCCATCTAAAAGAAGGCCATGTGTACATCAATACATGTACTGATATATACTCCCTCTGTCCAAAATAAATCAACTTTTGTACATGTCAA
CDS Sequence
  • >LOC_Os06g03580.3
    ATGGCCACCGTGGGGCAGCCTGATGCCATGAGAAGGATCACCGTTCACTACGTGAATCCGCCGCCGATCGCCGGCGCCGGAGAAGCACACGTCGATGGCCTCGACGACGAGGTTCTTGATTATGTGATTGGTGATGTTCTTCAGGATCAGGAGGGTCTGTATCAATCGATATTGTATGGCAAATACGGGGATGACATGAGAGGGGCAAGAAACACTGCTCTTGCCCAATCTGATGGTTTACATTACTACTATCACGGAGAGAACAGCAGTGGTGAAGCAACAACCTCGAGAAATTCAGAAATCGACCAACAGATTGAGTATGACTTGGTGTTCGCTAGGCAGCTGCAGGCAATGGATAATCTGACAATTGAGACACCTGCAGATGAAGATGATGATATAAGCTGCGTACCCTCTCCATCTGACTCTGAAACCGATGAACCGGCAGAAGGCAACAACGAAGAGGCTGCCACACAGGATGATAACGATGATCCAGACAACATGACATACGAGCAAAGGCAGGCACTTGTGGAATCTGTAGGAAATGAGAACAGAGGTTTATCTGATCTGCTTATCTCCTACTTGGAGACATGGAAGTACAAATCAGGCTTTTTCCCAAGGAAGGCAAACCATGATAACTGTCCTATATGTCTATCCGCTTTCAGACGCCGAGAAACACTCATTACATTGGCTTGCAAGCATAGTTACCATGAAGGTTGTATTGCTAGATGGCTCAAGATTGACAAGGTAAACACCTTCTTGGGACACTAG
  • >LOC_Os06g03580.1
    ATGGCCACCGTGGGGCAGCCTGATGCCATGAGAAGGATCACCGTTCACTACGTGAATCCGCCGCCGATCGCCGGCGCCGGAGAAGCACACGTCGATGGCCTCGACGACGAGGTTCTTGATTATGTGATTGGTGATGTTCTTCAGGATCAGGAGGGTCTGTATCAATCGATATTGTATGGCAAATACGGGGATGACATGAGAGGGGCAAGAAACACTGCTCTTGCCCAATCTGATGGTTTACATTACTACTATCACGGAGAGAACAGCAGTGGTGAAGCAACAACCTCGAGAAATTCAGAAATCGACCAACAGATTGAGTATGACTTGGTGTTCGCTAGGCAGCTGCAGGCAATGGATAATCTGACAATTGAGACACCTGCAGATGAAGATGATGATATAAGCTGCGTACCCTCTCCATCTGACTCTGAAACCGATGAACCGGCAGAAGGCAACAACGAAGAGGCTGCCACACAGGATGATAACGATGATCCAGACAACATGACATACGAGCAAAGGCAGGCACTTGTGGAATCTGTAGGAAATGAGAACAGAGGTTTATCTGATCTGCTTATCTCCTACTTGGAGACATGGAAGTACAAATCAGGCTTTTTCCCAAGGAAGGCAAACCATGATAACTGTCCTATATGTCTATCCGCTTTCAGACGCCGAGAAACACTCATTACATTGGCTTGCAAGCATAGTTACCATGAAGGTTGTATTGCTAGATGGCTCAAGATTGACAAGGTAAACACCTTCTTGGGACACTAG
  • >LOC_Os06g03580.4
    ATGGCCACCGTGGGGCAGCCTGATGCCATGAGAAGGATCACCGTTCACTACGTGAATCCGCCGCCGATCGCCGGCGCCGGAGAAGCACACGTCGATGGCCTCGACGACGAGGTTCTTGATTATGTGATTGGTGATGTTCTTCAGGATCAGGAGGGTCTGTATCAATCGATATTGTATGGCAAATACGGGGATGACATGAGAGGGGCAAGAAACACTGCTCTTGCCCAATCTGATGGTTTACATTACTACTATCACGGAGAGAACAGCAGTGGTGAAGCAACAACCTCGAGAAATTCAGAAATCGACCAACAGATTGAGTATGACTTGGTGTTCGCTAGGCAGCTGCAGGCAATGGATAATCTGACAATTGAGACACCTGCAGATGAAGATGATGATATAAGCTGCGTACCCTCTCCATCTGACTCTGAAACCGATGAACCGGCAGAAGGCAACAACGAAGAGGCTGCCACACAGGATGATAACGATGATCCAGACAACATGACATACGAGCAAAGGCAGGCACTTGTGGAATCTGTAGGAAATGAGAACAGAGGTTTATCTGATCTGCTTATCTCCTACTTGGAGACATGGAAGTACAAATCAGGCTTTTTCCCAAGGAAGGCAAACCATGATAACTGTCCTATATGTCTATCCGCTTTCAGACGCCGAGAAACACTCATTACATTGGCTTGCAAGCATAGTTACCATGAAGGTTGTATTGCTAGATGGCTCAAGATTGACAAGGTAAACACCTTCTTGGGACACTAG
  • >LOC_Os06g03580.2
    ATGGCCACCGTGGGGCAGCCTGATGCCATGAGAAGGATCACCGTTCACTACGTGAATCCGCCGCCGATCGCCGGCGCCGGAGAAGCACACGTCGATGGCCTCGACGACGAGGTTCTTGATTATGTGATTGGTGATGTTCTTCAGGATCAGGAGGGTCTGTATCAATCGATATTGTATGGCAAATACGGGGATGACATGAGAGGGGCAAGAAACACTGCTCTTGCCCAATCTGATGGTTTACATTACTACTATCACGGAGAGAACAGCAGTGGTGAAGCAACAACCTCGAGAAATTCAGAAATCGACCAACAGATTGAGTATGACTTGGTGTTCGCTAGGCAGCTGCAGGCAATGGATAATCTGACAATTGAGACACCTGCAGATGAAGATGATGATATAAGCTGCGTACCCTCTCCATCTGACTCTGAAACCGATGAACCGGCAGAAGGCAACAACGAAGAGGCTGCCACACAGGATGATAACGATGATCCAGACAACATGACATACGAGCAAAGGCAGGCACTTGTGGAATCTGTAGGAAATGAGAACAGAGGTTTATCTGATCTGCTTATCTCCTACTTGGAGACATGGAAGTACAAATCAGGCTTTTTCCCAAGGAAGGCAAACCATGATAACTGTCCTATATGTCTATCCGCTTTCAGACGCCGAGAAACACTCATTACATTGGCTTGCAAGCATAGTTACCATGAAGGTTGTATTGCTAGATGGCTCAAGATTGACAAGGTAAACACCTTCTTGGGACACTAG
Protein Sequence
  • >LOC_Os06g03580.3
    MATVGQPDAMRRITVHYVNPPPIAGAGEAHVDGLDDEVLDYVIGDVLQDQEGLYQSILYGKYGDDMRGARNTALAQSDGLHYYYHGENSSGEATTSRNSEIDQQIEYDLVFARQLQAMDNLTIETPADEDDDISCVPSPSDSETDEPAEGNNEEAATQDDNDDPDNMTYEQRQALVESVGNENRGLSDLLISYLETWKYKSGFFPRKANHDNCPICLSAFRRRETLITLACKHSYHEGCIARWLKIDKVNTFLGH*
  • >LOC_Os06g03580.1
    MATVGQPDAMRRITVHYVNPPPIAGAGEAHVDGLDDEVLDYVIGDVLQDQEGLYQSILYGKYGDDMRGARNTALAQSDGLHYYYHGENSSGEATTSRNSEIDQQIEYDLVFARQLQAMDNLTIETPADEDDDISCVPSPSDSETDEPAEGNNEEAATQDDNDDPDNMTYEQRQALVESVGNENRGLSDLLISYLETWKYKSGFFPRKANHDNCPICLSAFRRRETLITLACKHSYHEGCIARWLKIDKVNTFLGH*
  • >LOC_Os06g03580.4
    MATVGQPDAMRRITVHYVNPPPIAGAGEAHVDGLDDEVLDYVIGDVLQDQEGLYQSILYGKYGDDMRGARNTALAQSDGLHYYYHGENSSGEATTSRNSEIDQQIEYDLVFARQLQAMDNLTIETPADEDDDISCVPSPSDSETDEPAEGNNEEAATQDDNDDPDNMTYEQRQALVESVGNENRGLSDLLISYLETWKYKSGFFPRKANHDNCPICLSAFRRRETLITLACKHSYHEGCIARWLKIDKVNTFLGH*
  • >LOC_Os06g03580.2
    MATVGQPDAMRRITVHYVNPPPIAGAGEAHVDGLDDEVLDYVIGDVLQDQEGLYQSILYGKYGDDMRGARNTALAQSDGLHYYYHGENSSGEATTSRNSEIDQQIEYDLVFARQLQAMDNLTIETPADEDDDISCVPSPSDSETDEPAEGNNEEAATQDDNDDPDNMTYEQRQALVESVGNENRGLSDLLISYLETWKYKSGFFPRKANHDNCPICLSAFRRRETLITLACKHSYHEGCIARWLKIDKVNTFLGH*