Information report for RA1
Gene Details
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Functional Descriptions
- By transient expression in Nicotiana benthamiana, we found that RA1 protein was localized in the chloroplast.
- RNA analysis revealed biogenesis of chloroplast rRNAs was abnormal in RA1.
- These results suggest that RA1 is involved in early chloroplast development and establishment of the plastidic ribosome system in rice.
- Expression levels of chlorophyll biosynthesis and plastid development related genes were disordered in the RA1 mutant.
- Map-based cloning revealed that RA1 gene encodes a glycyl-tRNA synthetase (GlyRS), which was confirmed by genetic complementation and knockout by Crispr/Cas9 technology.
Functional Keywords
Literature and News
Gene Resources
- UniProt: Q0DFB6
- EMBL: AP014962, CM000143
- EnsemblPlants: Os06t0103600-01
- Gramene: Os06t0103600-01
- KEGG: osa:4339847
- Orthologous matrix: LPIPKRM
- InterPro: IPR002310, IPR006194, IPR008909
- PANTHER: PTHR30075, PTHR30075:SF2
- SUPFAM: SSF109604, SSF55681
- PROSITE: PS50861
- Gene3D: 1.20.58.180, 3.30.930.10
- OrthoDB: Q0DFB6
- SWISS-MODEL: Q0DFB6
- Conserved Domain Database: cd00733
- eggNOG: ENOG502QS5T
Homologs
- Arabidopsis thaliana: AT3G48110
- Lactuca sativa: Lsat_1_v5_gn_1_90880
Sequences
cDNA Sequence
- >LOC_Os06g01400.1
GGAGTCCGTCCAGGTCCAGGTCCAGTGTAGTCGTAGCCATGGCGGGTTTGGCTCCCCCTCCCCTCCTCCTCCTCCCCCTCTCCTCCTCACTCTCTCCTGCCGCCGCCTCCCACCACCCAACCTCCCCCCCTTCTCGCACACGCCGACGACGCCGCCTGCTATCCGCCGTGGCGTCTGCCGACGGTGATGCTCCCTCCCCAGTCTCTGTCTCCGCCTCCGCCGCCACCAAGGGGCCTTCCTCCTCCTCCGTGCTCACCTTCCAGCAGGCCATCCAGCGCCTCCAGGACTACTGGGCCTCAGTGGGGTGTGCCGTCATGCAGTGCAGCAATACAGAGGTTGGAGCAGGGACCATGAATCCCTTGACATTCCTCAGGGTTTTAGGCCCCGAGCCATGGAACGTTGCTTATGTGGAGCCAAGTATAAGACCCGACGACAGCCGCTATGGGGACAATCCTAATAGGCTTCAGCGTCATACTCAATTTCAGGTAATTTTGAAGCCTGACCCTGGAAATTCACAAGACCTCTTTCTGCACAGCCTTTCAGCACTAGGTATCAATGTCCGTGAACATGACATCCGTTTTGTTGAAGACAACTGGGAGAGTCCAGTTCTTGGTGCGTGGGGATTAGGCTGGGAGGTTTGGATGGATGGCATGGAAATAACACAGTTCACATATTTTCAGCAGTCAGGAAGCCTTCCATTGCTGCCTGTTTCTGTTGAGATAACATATGGACTTGAACGCATCCTCATGTCTCTTCAGGGTGTAGATCATTTCAAGAATATACAATACACTAAAGGAATTACATATGGAGAACTATTCCTTGAGAATGAGAAAGAGATGAGTGCATATTATTTGGAGCATGCAAATGTTGATAACATTCAAAAGCATTTTGATGATTTTGAAGAAGAGGCTCGCTCTTTATTATCACTTTGGCTTCCTATTCCTGCGTACGATCATGTTCTGAAGGCCTCTCATGCTTTTAATATTTTAGATTCTAGAGGCTTTGTTGGTGTAACCGAGCGTGCAAGATATTTTGGTCGCATGCGGAGTCTTGCTCGCCAGTGTGCTCAGCTTTGGGTGAAAACAAGAGAGAACCTCGGTTATCCTTTGGGCACTTATCAAGAGTCTAATCTTATCTATCCTCATGTCTCTGAAAAGCCTAGTAGAAAGGGAGTTGTCGGACAGCCACGAGCATTTGTTCTTGAAATAGGAACAGAAGAGTTGCCACCTCATGATGTGATAGAAGCAACCAAACAGCTGGAGAAATCTTTAATTCAAATATTGGAGAAACGAAGACTGAGCCATGGGAAAGTACGCTCTTATGGGACACCAAGGAGATTAGCGGTTGTTGTTGAAAATCTTAATATGAAGCAAATGGAGGAAGAGATTGAACTACGTGGTCCACCTGTTGCAAAGGCATTTGACCAAGAAGGAAGACCCACTAAGGCTGCTGAAGGATTTTGTCGAAAGAATAATGTCCTGATAGATAGTCTATATAGAAGAACTGATGGTAAAACAGAATATATCTACGCTCGGGTCAAAGAGTCTGCACGTTTTGCTGATGAGGTACTAACTGAAGATCTACCGACTATTATATCTGGTATTTCATTTCCCAAGTCTATGCGCTGGAACTCCAATATTGTGTTTAGCAGGCCAATACGCTGGATCTTTGCCCTTCATGGGGATCTCATTGTGCCATTTTGCTTTGCTGGCATATCAAGTGGGAATCAGTCGTGTGGCCTTCGTAACTCCTCCTTGGCTAACTTTAAGGTGGAAGCTGCAGAATTATATTTACACACCCTGGAGAAAGCTGGGATCCTGATTGATATGCAGGAGCGCAAACAACGAATCTTGCATGACTCTAGTATATTGGCTGAGGGTGTTGGCGGTGATATCATTGCACCTGATAGCTTGGTGCAGGAGGTTATCAATCTTGTGGAGGCGCCGATGCCCATTATTGGTCGATACGATGTTTCCTTCTTAGCACTTCCAAAGGATGTATTAATAACGGTTATGCAGAAACACCAGAAATATTTCCCTGTAACCTCCAAGACCATGGGCAATCTGCTACCATGTTTCATAACTGTTGCCAATGGTGCCATTAAGGAAGAAGTAGTCCGCAAAGGGAATGAAGCTGTACTCAGGGCACGGTATGAGGATGCTAAATTCTTTTATAAGATGGATACGCAGAAGAAACTTTCTGAATTCCGAGATCAGCTCAGCAGCATTCTCTTTCATGAAAGACTTGGTACCATGCTTGACAAAATGAAACGTGTTGAGAACACTGTTGCAGAAGTGGCCCTCCTTCTCGGAATTAATGAGAAAATGATCCCAGCAATCAAAGATGCAGCTGCATTGGCTATGTCAGATCTTGCCACTAATATTGTTACTGAATTTACTTCACTTGCTGGAATTATGGCACGGCATTATGCTTTGAGAGATGGCCTTTCAGAACAGATTGCAGAAGCCCTATTTGAGATAACACTTCCAAGGTTCTCTGGTGATGTGTTCCCAAAAACAGATCCTGGCATAGTCCTTGCAGTCACTGACAGATTGGATAGTCTAGTGGGTCTGTTTGGAGCTGGATGCCAACCAAGTTCTACAAATGATCCATTTGGGCTAAGGAGAATTTCCTATGGATTGGTTCAAATATTGGTTGAGAACAAGAAGAACTTTGACCTCACGAAGGCCTTGACCTTAGTGGCAGAGGAGCAGCCAATAACAATAGATAGTGGTGTTATAGATGAGGTAGTACAATTTGTCACGCGGAGATTGGAGCAACTTTTGGTGGATGAAGGAATTAATTGCGAGATAGTCCGTTCAGTGCTTATAGAGCGTGCAAACTGCCCTTATCTAGCATCACAAACAGCAATTGAAATGGAAGCATTTTCAAGAACTGAAGATTTCCCAAAAATTGTCGAAGCGTACTCAAGGCCCGCGAGAATAATACGTGGAAAAGAAATTGGGTCTGCTTTAGAGGTTGATGCAAGTGTATTTGAGAAAGACGAAGAAAGAGCTTTGTGGAGTGCCTACTTGGAGGTTGCGGATAAGATTCATCCTGGTGTTGACATAAAAGCCTTTGCGGACGCTTCTCTGGAACTGCTGCAACCCCTAGAAGATTTCTTTACAAATGTTTTTGTCATGGCGGAAGACGAGAGAGTTCGTAACAATCGGCTTGCACTGCTAACGAAGGTTGCGAGTCTGCCAAAGGGAATTGCCGACCTGTCAGTTTTACCAGGGTTTTAGCCATTTTTTGTTGCTCTTCTAGTTTTATCTGTACCATGCTTGTACATTCATACCGACTACAGTTTTGCAGCCAAGAAAAGAGCGTGATCCCTATATATATAATGTATAGGTAGCTAGCATAAATTGTCCTCAACTTTGTTGTCCATTGCTATGTTAAACATATGTTTTTGTTTTCAAACCATTTTGGAAATCAAATGCCATAACTTGTATCAGACTATATACCAACTCAAGCGGTATCTTGCATAAATTCCATTCTATTCCC
CDS Sequence
- >LOC_Os06g01400.1
ATGGCGGGTTTGGCTCCCCCTCCCCTCCTCCTCCTCCCCCTCTCCTCCTCACTCTCTCCTGCCGCCGCCTCCCACCACCCAACCTCCCCCCCTTCTCGCACACGCCGACGACGCCGCCTGCTATCCGCCGTGGCGTCTGCCGACGGTGATGCTCCCTCCCCAGTCTCTGTCTCCGCCTCCGCCGCCACCAAGGGGCCTTCCTCCTCCTCCGTGCTCACCTTCCAGCAGGCCATCCAGCGCCTCCAGGACTACTGGGCCTCAGTGGGGTGTGCCGTCATGCAGTGCAGCAATACAGAGGTTGGAGCAGGGACCATGAATCCCTTGACATTCCTCAGGGTTTTAGGCCCCGAGCCATGGAACGTTGCTTATGTGGAGCCAAGTATAAGACCCGACGACAGCCGCTATGGGGACAATCCTAATAGGCTTCAGCGTCATACTCAATTTCAGGTAATTTTGAAGCCTGACCCTGGAAATTCACAAGACCTCTTTCTGCACAGCCTTTCAGCACTAGGTATCAATGTCCGTGAACATGACATCCGTTTTGTTGAAGACAACTGGGAGAGTCCAGTTCTTGGTGCGTGGGGATTAGGCTGGGAGGTTTGGATGGATGGCATGGAAATAACACAGTTCACATATTTTCAGCAGTCAGGAAGCCTTCCATTGCTGCCTGTTTCTGTTGAGATAACATATGGACTTGAACGCATCCTCATGTCTCTTCAGGGTGTAGATCATTTCAAGAATATACAATACACTAAAGGAATTACATATGGAGAACTATTCCTTGAGAATGAGAAAGAGATGAGTGCATATTATTTGGAGCATGCAAATGTTGATAACATTCAAAAGCATTTTGATGATTTTGAAGAAGAGGCTCGCTCTTTATTATCACTTTGGCTTCCTATTCCTGCGTACGATCATGTTCTGAAGGCCTCTCATGCTTTTAATATTTTAGATTCTAGAGGCTTTGTTGGTGTAACCGAGCGTGCAAGATATTTTGGTCGCATGCGGAGTCTTGCTCGCCAGTGTGCTCAGCTTTGGGTGAAAACAAGAGAGAACCTCGGTTATCCTTTGGGCACTTATCAAGAGTCTAATCTTATCTATCCTCATGTCTCTGAAAAGCCTAGTAGAAAGGGAGTTGTCGGACAGCCACGAGCATTTGTTCTTGAAATAGGAACAGAAGAGTTGCCACCTCATGATGTGATAGAAGCAACCAAACAGCTGGAGAAATCTTTAATTCAAATATTGGAGAAACGAAGACTGAGCCATGGGAAAGTACGCTCTTATGGGACACCAAGGAGATTAGCGGTTGTTGTTGAAAATCTTAATATGAAGCAAATGGAGGAAGAGATTGAACTACGTGGTCCACCTGTTGCAAAGGCATTTGACCAAGAAGGAAGACCCACTAAGGCTGCTGAAGGATTTTGTCGAAAGAATAATGTCCTGATAGATAGTCTATATAGAAGAACTGATGGTAAAACAGAATATATCTACGCTCGGGTCAAAGAGTCTGCACGTTTTGCTGATGAGGTACTAACTGAAGATCTACCGACTATTATATCTGGTATTTCATTTCCCAAGTCTATGCGCTGGAACTCCAATATTGTGTTTAGCAGGCCAATACGCTGGATCTTTGCCCTTCATGGGGATCTCATTGTGCCATTTTGCTTTGCTGGCATATCAAGTGGGAATCAGTCGTGTGGCCTTCGTAACTCCTCCTTGGCTAACTTTAAGGTGGAAGCTGCAGAATTATATTTACACACCCTGGAGAAAGCTGGGATCCTGATTGATATGCAGGAGCGCAAACAACGAATCTTGCATGACTCTAGTATATTGGCTGAGGGTGTTGGCGGTGATATCATTGCACCTGATAGCTTGGTGCAGGAGGTTATCAATCTTGTGGAGGCGCCGATGCCCATTATTGGTCGATACGATGTTTCCTTCTTAGCACTTCCAAAGGATGTATTAATAACGGTTATGCAGAAACACCAGAAATATTTCCCTGTAACCTCCAAGACCATGGGCAATCTGCTACCATGTTTCATAACTGTTGCCAATGGTGCCATTAAGGAAGAAGTAGTCCGCAAAGGGAATGAAGCTGTACTCAGGGCACGGTATGAGGATGCTAAATTCTTTTATAAGATGGATACGCAGAAGAAACTTTCTGAATTCCGAGATCAGCTCAGCAGCATTCTCTTTCATGAAAGACTTGGTACCATGCTTGACAAAATGAAACGTGTTGAGAACACTGTTGCAGAAGTGGCCCTCCTTCTCGGAATTAATGAGAAAATGATCCCAGCAATCAAAGATGCAGCTGCATTGGCTATGTCAGATCTTGCCACTAATATTGTTACTGAATTTACTTCACTTGCTGGAATTATGGCACGGCATTATGCTTTGAGAGATGGCCTTTCAGAACAGATTGCAGAAGCCCTATTTGAGATAACACTTCCAAGGTTCTCTGGTGATGTGTTCCCAAAAACAGATCCTGGCATAGTCCTTGCAGTCACTGACAGATTGGATAGTCTAGTGGGTCTGTTTGGAGCTGGATGCCAACCAAGTTCTACAAATGATCCATTTGGGCTAAGGAGAATTTCCTATGGATTGGTTCAAATATTGGTTGAGAACAAGAAGAACTTTGACCTCACGAAGGCCTTGACCTTAGTGGCAGAGGAGCAGCCAATAACAATAGATAGTGGTGTTATAGATGAGGTAGTACAATTTGTCACGCGGAGATTGGAGCAACTTTTGGTGGATGAAGGAATTAATTGCGAGATAGTCCGTTCAGTGCTTATAGAGCGTGCAAACTGCCCTTATCTAGCATCACAAACAGCAATTGAAATGGAAGCATTTTCAAGAACTGAAGATTTCCCAAAAATTGTCGAAGCGTACTCAAGGCCCGCGAGAATAATACGTGGAAAAGAAATTGGGTCTGCTTTAGAGGTTGATGCAAGTGTATTTGAGAAAGACGAAGAAAGAGCTTTGTGGAGTGCCTACTTGGAGGTTGCGGATAAGATTCATCCTGGTGTTGACATAAAAGCCTTTGCGGACGCTTCTCTGGAACTGCTGCAACCCCTAGAAGATTTCTTTACAAATGTTTTTGTCATGGCGGAAGACGAGAGAGTTCGTAACAATCGGCTTGCACTGCTAACGAAGGTTGCGAGTCTGCCAAAGGGAATTGCCGACCTGTCAGTTTTACCAGGGTTTTAG
Protein Sequence
- >LOC_Os06g01400.1
MAGLAPPPLLLLPLSSSLSPAAASHHPTSPPSRTRRRRRLLSAVASADGDAPSPVSVSASAATKGPSSSSVLTFQQAIQRLQDYWASVGCAVMQCSNTEVGAGTMNPLTFLRVLGPEPWNVAYVEPSIRPDDSRYGDNPNRLQRHTQFQVILKPDPGNSQDLFLHSLSALGINVREHDIRFVEDNWESPVLGAWGLGWEVWMDGMEITQFTYFQQSGSLPLLPVSVEITYGLERILMSLQGVDHFKNIQYTKGITYGELFLENEKEMSAYYLEHANVDNIQKHFDDFEEEARSLLSLWLPIPAYDHVLKASHAFNILDSRGFVGVTERARYFGRMRSLARQCAQLWVKTRENLGYPLGTYQESNLIYPHVSEKPSRKGVVGQPRAFVLEIGTEELPPHDVIEATKQLEKSLIQILEKRRLSHGKVRSYGTPRRLAVVVENLNMKQMEEEIELRGPPVAKAFDQEGRPTKAAEGFCRKNNVLIDSLYRRTDGKTEYIYARVKESARFADEVLTEDLPTIISGISFPKSMRWNSNIVFSRPIRWIFALHGDLIVPFCFAGISSGNQSCGLRNSSLANFKVEAAELYLHTLEKAGILIDMQERKQRILHDSSILAEGVGGDIIAPDSLVQEVINLVEAPMPIIGRYDVSFLALPKDVLITVMQKHQKYFPVTSKTMGNLLPCFITVANGAIKEEVVRKGNEAVLRARYEDAKFFYKMDTQKKLSEFRDQLSSILFHERLGTMLDKMKRVENTVAEVALLLGINEKMIPAIKDAAALAMSDLATNIVTEFTSLAGIMARHYALRDGLSEQIAEALFEITLPRFSGDVFPKTDPGIVLAVTDRLDSLVGLFGAGCQPSSTNDPFGLRRISYGLVQILVENKKNFDLTKALTLVAEEQPITIDSGVIDEVVQFVTRRLEQLLVDEGINCEIVRSVLIERANCPYLASQTAIEMEAFSRTEDFPKIVEAYSRPARIIRGKEIGSALEVDASVFEKDEERALWSAYLEVADKIHPGVDIKAFADASLELLQPLEDFFTNVFVMAEDERVRNNRLALLTKVASLPKGIADLSVLPGF*