Gene Details:

Functional Descriptions:

  • By transient expression in Nicotiana benthamiana, we found that RA1 protein was localized in the chloroplast.
  • RNA analysis revealed biogenesis of chloroplast rRNAs was abnormal in RA1.
  • These results suggest that RA1 is involved in early chloroplast development and establishment of the plastidic ribosome system in rice.
  • Expression levels of chlorophyll biosynthesis and plastid development related genes were disordered in the RA1 mutant.
  • Map-based cloning revealed that RA1 gene encodes a glycyl-tRNA synthetase (GlyRS), which was confirmed by genetic complementation and knockout by Crispr/Cas9 technology.

Literature:

Gene Resources:

  • NCBI ID:
  • UniProt accessions:

Sequences:

cDNA Sequence
  • >LOC_Os06g01400.1
    GGAGTCCGTCCAGGTCCAGGTCCAGTGTAGTCGTAGCCATGGCGGGTTTGGCTCCCCCTCCCCTCCTCCTCCTCCCCCTCTCCTCCTCACTCTCTCCTGCCGCCGCCTCCCACCACCCAACCTCCCCCCCTTCTCGCACACGCCGACGACGCCGCCTGCTATCCGCCGTGGCGTCTGCCGACGGTGATGCTCCCTCCCCAGTCTCTGTCTCCGCCTCCGCCGCCACCAAGGGGCCTTCCTCCTCCTCCGTGCTCACCTTCCAGCAGGCCATCCAGCGCCTCCAGGACTACTGGGCCTCAGTGGGGTGTGCCGTCATGCAGTGCAGCAATACAGAGGTTGGAGCAGGGACCATGAATCCCTTGACATTCCTCAGGGTTTTAGGCCCCGAGCCATGGAACGTTGCTTATGTGGAGCCAAGTATAAGACCCGACGACAGCCGCTATGGGGACAATCCTAATAGGCTTCAGCGTCATACTCAATTTCAGGTAATTTTGAAGCCTGACCCTGGAAATTCACAAGACCTCTTTCTGCACAGCCTTTCAGCACTAGGTATCAATGTCCGTGAACATGACATCCGTTTTGTTGAAGACAACTGGGAGAGTCCAGTTCTTGGTGCGTGGGGATTAGGCTGGGAGGTTTGGATGGATGGCATGGAAATAACACAGTTCACATATTTTCAGCAGTCAGGAAGCCTTCCATTGCTGCCTGTTTCTGTTGAGATAACATATGGACTTGAACGCATCCTCATGTCTCTTCAGGGTGTAGATCATTTCAAGAATATACAATACACTAAAGGAATTACATATGGAGAACTATTCCTTGAGAATGAGAAAGAGATGAGTGCATATTATTTGGAGCATGCAAATGTTGATAACATTCAAAAGCATTTTGATGATTTTGAAGAAGAGGCTCGCTCTTTATTATCACTTTGGCTTCCTATTCCTGCGTACGATCATGTTCTGAAGGCCTCTCATGCTTTTAATATTTTAGATTCTAGAGGCTTTGTTGGTGTAACCGAGCGTGCAAGATATTTTGGTCGCATGCGGAGTCTTGCTCGCCAGTGTGCTCAGCTTTGGGTGAAAACAAGAGAGAACCTCGGTTATCCTTTGGGCACTTATCAAGAGTCTAATCTTATCTATCCTCATGTCTCTGAAAAGCCTAGTAGAAAGGGAGTTGTCGGACAGCCACGAGCATTTGTTCTTGAAATAGGAACAGAAGAGTTGCCACCTCATGATGTGATAGAAGCAACCAAACAGCTGGAGAAATCTTTAATTCAAATATTGGAGAAACGAAGACTGAGCCATGGGAAAGTACGCTCTTATGGGACACCAAGGAGATTAGCGGTTGTTGTTGAAAATCTTAATATGAAGCAAATGGAGGAAGAGATTGAACTACGTGGTCCACCTGTTGCAAAGGCATTTGACCAAGAAGGAAGACCCACTAAGGCTGCTGAAGGATTTTGTCGAAAGAATAATGTCCTGATAGATAGTCTATATAGAAGAACTGATGGTAAAACAGAATATATCTACGCTCGGGTCAAAGAGTCTGCACGTTTTGCTGATGAGGTACTAACTGAAGATCTACCGACTATTATATCTGGTATTTCATTTCCCAAGTCTATGCGCTGGAACTCCAATATTGTGTTTAGCAGGCCAATACGCTGGATCTTTGCCCTTCATGGGGATCTCATTGTGCCATTTTGCTTTGCTGGCATATCAAGTGGGAATCAGTCGTGTGGCCTTCGTAACTCCTCCTTGGCTAACTTTAAGGTGGAAGCTGCAGAATTATATTTACACACCCTGGAGAAAGCTGGGATCCTGATTGATATGCAGGAGCGCAAACAACGAATCTTGCATGACTCTAGTATATTGGCTGAGGGTGTTGGCGGTGATATCATTGCACCTGATAGCTTGGTGCAGGAGGTTATCAATCTTGTGGAGGCGCCGATGCCCATTATTGGTCGATACGATGTTTCCTTCTTAGCACTTCCAAAGGATGTATTAATAACGGTTATGCAGAAACACCAGAAATATTTCCCTGTAACCTCCAAGACCATGGGCAATCTGCTACCATGTTTCATAACTGTTGCCAATGGTGCCATTAAGGAAGAAGTAGTCCGCAAAGGGAATGAAGCTGTACTCAGGGCACGGTATGAGGATGCTAAATTCTTTTATAAGATGGATACGCAGAAGAAACTTTCTGAATTCCGAGATCAGCTCAGCAGCATTCTCTTTCATGAAAGACTTGGTACCATGCTTGACAAAATGAAACGTGTTGAGAACACTGTTGCAGAAGTGGCCCTCCTTCTCGGAATTAATGAGAAAATGATCCCAGCAATCAAAGATGCAGCTGCATTGGCTATGTCAGATCTTGCCACTAATATTGTTACTGAATTTACTTCACTTGCTGGAATTATGGCACGGCATTATGCTTTGAGAGATGGCCTTTCAGAACAGATTGCAGAAGCCCTATTTGAGATAACACTTCCAAGGTTCTCTGGTGATGTGTTCCCAAAAACAGATCCTGGCATAGTCCTTGCAGTCACTGACAGATTGGATAGTCTAGTGGGTCTGTTTGGAGCTGGATGCCAACCAAGTTCTACAAATGATCCATTTGGGCTAAGGAGAATTTCCTATGGATTGGTTCAAATATTGGTTGAGAACAAGAAGAACTTTGACCTCACGAAGGCCTTGACCTTAGTGGCAGAGGAGCAGCCAATAACAATAGATAGTGGTGTTATAGATGAGGTAGTACAATTTGTCACGCGGAGATTGGAGCAACTTTTGGTGGATGAAGGAATTAATTGCGAGATAGTCCGTTCAGTGCTTATAGAGCGTGCAAACTGCCCTTATCTAGCATCACAAACAGCAATTGAAATGGAAGCATTTTCAAGAACTGAAGATTTCCCAAAAATTGTCGAAGCGTACTCAAGGCCCGCGAGAATAATACGTGGAAAAGAAATTGGGTCTGCTTTAGAGGTTGATGCAAGTGTATTTGAGAAAGACGAAGAAAGAGCTTTGTGGAGTGCCTACTTGGAGGTTGCGGATAAGATTCATCCTGGTGTTGACATAAAAGCCTTTGCGGACGCTTCTCTGGAACTGCTGCAACCCCTAGAAGATTTCTTTACAAATGTTTTTGTCATGGCGGAAGACGAGAGAGTTCGTAACAATCGGCTTGCACTGCTAACGAAGGTTGCGAGTCTGCCAAAGGGAATTGCCGACCTGTCAGTTTTACCAGGGTTTTAGCCATTTTTTGTTGCTCTTCTAGTTTTATCTGTACCATGCTTGTACATTCATACCGACTACAGTTTTGCAGCCAAGAAAAGAGCGTGATCCCTATATATATAATGTATAGGTAGCTAGCATAAATTGTCCTCAACTTTGTTGTCCATTGCTATGTTAAACATATGTTTTTGTTTTCAAACCATTTTGGAAATCAAATGCCATAACTTGTATCAGACTATATACCAACTCAAGCGGTATCTTGCATAAATTCCATTCTATTCCC
CDS Sequence
  • >LOC_Os06g01400.1
    ATGGCGGGTTTGGCTCCCCCTCCCCTCCTCCTCCTCCCCCTCTCCTCCTCACTCTCTCCTGCCGCCGCCTCCCACCACCCAACCTCCCCCCCTTCTCGCACACGCCGACGACGCCGCCTGCTATCCGCCGTGGCGTCTGCCGACGGTGATGCTCCCTCCCCAGTCTCTGTCTCCGCCTCCGCCGCCACCAAGGGGCCTTCCTCCTCCTCCGTGCTCACCTTCCAGCAGGCCATCCAGCGCCTCCAGGACTACTGGGCCTCAGTGGGGTGTGCCGTCATGCAGTGCAGCAATACAGAGGTTGGAGCAGGGACCATGAATCCCTTGACATTCCTCAGGGTTTTAGGCCCCGAGCCATGGAACGTTGCTTATGTGGAGCCAAGTATAAGACCCGACGACAGCCGCTATGGGGACAATCCTAATAGGCTTCAGCGTCATACTCAATTTCAGGTAATTTTGAAGCCTGACCCTGGAAATTCACAAGACCTCTTTCTGCACAGCCTTTCAGCACTAGGTATCAATGTCCGTGAACATGACATCCGTTTTGTTGAAGACAACTGGGAGAGTCCAGTTCTTGGTGCGTGGGGATTAGGCTGGGAGGTTTGGATGGATGGCATGGAAATAACACAGTTCACATATTTTCAGCAGTCAGGAAGCCTTCCATTGCTGCCTGTTTCTGTTGAGATAACATATGGACTTGAACGCATCCTCATGTCTCTTCAGGGTGTAGATCATTTCAAGAATATACAATACACTAAAGGAATTACATATGGAGAACTATTCCTTGAGAATGAGAAAGAGATGAGTGCATATTATTTGGAGCATGCAAATGTTGATAACATTCAAAAGCATTTTGATGATTTTGAAGAAGAGGCTCGCTCTTTATTATCACTTTGGCTTCCTATTCCTGCGTACGATCATGTTCTGAAGGCCTCTCATGCTTTTAATATTTTAGATTCTAGAGGCTTTGTTGGTGTAACCGAGCGTGCAAGATATTTTGGTCGCATGCGGAGTCTTGCTCGCCAGTGTGCTCAGCTTTGGGTGAAAACAAGAGAGAACCTCGGTTATCCTTTGGGCACTTATCAAGAGTCTAATCTTATCTATCCTCATGTCTCTGAAAAGCCTAGTAGAAAGGGAGTTGTCGGACAGCCACGAGCATTTGTTCTTGAAATAGGAACAGAAGAGTTGCCACCTCATGATGTGATAGAAGCAACCAAACAGCTGGAGAAATCTTTAATTCAAATATTGGAGAAACGAAGACTGAGCCATGGGAAAGTACGCTCTTATGGGACACCAAGGAGATTAGCGGTTGTTGTTGAAAATCTTAATATGAAGCAAATGGAGGAAGAGATTGAACTACGTGGTCCACCTGTTGCAAAGGCATTTGACCAAGAAGGAAGACCCACTAAGGCTGCTGAAGGATTTTGTCGAAAGAATAATGTCCTGATAGATAGTCTATATAGAAGAACTGATGGTAAAACAGAATATATCTACGCTCGGGTCAAAGAGTCTGCACGTTTTGCTGATGAGGTACTAACTGAAGATCTACCGACTATTATATCTGGTATTTCATTTCCCAAGTCTATGCGCTGGAACTCCAATATTGTGTTTAGCAGGCCAATACGCTGGATCTTTGCCCTTCATGGGGATCTCATTGTGCCATTTTGCTTTGCTGGCATATCAAGTGGGAATCAGTCGTGTGGCCTTCGTAACTCCTCCTTGGCTAACTTTAAGGTGGAAGCTGCAGAATTATATTTACACACCCTGGAGAAAGCTGGGATCCTGATTGATATGCAGGAGCGCAAACAACGAATCTTGCATGACTCTAGTATATTGGCTGAGGGTGTTGGCGGTGATATCATTGCACCTGATAGCTTGGTGCAGGAGGTTATCAATCTTGTGGAGGCGCCGATGCCCATTATTGGTCGATACGATGTTTCCTTCTTAGCACTTCCAAAGGATGTATTAATAACGGTTATGCAGAAACACCAGAAATATTTCCCTGTAACCTCCAAGACCATGGGCAATCTGCTACCATGTTTCATAACTGTTGCCAATGGTGCCATTAAGGAAGAAGTAGTCCGCAAAGGGAATGAAGCTGTACTCAGGGCACGGTATGAGGATGCTAAATTCTTTTATAAGATGGATACGCAGAAGAAACTTTCTGAATTCCGAGATCAGCTCAGCAGCATTCTCTTTCATGAAAGACTTGGTACCATGCTTGACAAAATGAAACGTGTTGAGAACACTGTTGCAGAAGTGGCCCTCCTTCTCGGAATTAATGAGAAAATGATCCCAGCAATCAAAGATGCAGCTGCATTGGCTATGTCAGATCTTGCCACTAATATTGTTACTGAATTTACTTCACTTGCTGGAATTATGGCACGGCATTATGCTTTGAGAGATGGCCTTTCAGAACAGATTGCAGAAGCCCTATTTGAGATAACACTTCCAAGGTTCTCTGGTGATGTGTTCCCAAAAACAGATCCTGGCATAGTCCTTGCAGTCACTGACAGATTGGATAGTCTAGTGGGTCTGTTTGGAGCTGGATGCCAACCAAGTTCTACAAATGATCCATTTGGGCTAAGGAGAATTTCCTATGGATTGGTTCAAATATTGGTTGAGAACAAGAAGAACTTTGACCTCACGAAGGCCTTGACCTTAGTGGCAGAGGAGCAGCCAATAACAATAGATAGTGGTGTTATAGATGAGGTAGTACAATTTGTCACGCGGAGATTGGAGCAACTTTTGGTGGATGAAGGAATTAATTGCGAGATAGTCCGTTCAGTGCTTATAGAGCGTGCAAACTGCCCTTATCTAGCATCACAAACAGCAATTGAAATGGAAGCATTTTCAAGAACTGAAGATTTCCCAAAAATTGTCGAAGCGTACTCAAGGCCCGCGAGAATAATACGTGGAAAAGAAATTGGGTCTGCTTTAGAGGTTGATGCAAGTGTATTTGAGAAAGACGAAGAAAGAGCTTTGTGGAGTGCCTACTTGGAGGTTGCGGATAAGATTCATCCTGGTGTTGACATAAAAGCCTTTGCGGACGCTTCTCTGGAACTGCTGCAACCCCTAGAAGATTTCTTTACAAATGTTTTTGTCATGGCGGAAGACGAGAGAGTTCGTAACAATCGGCTTGCACTGCTAACGAAGGTTGCGAGTCTGCCAAAGGGAATTGCCGACCTGTCAGTTTTACCAGGGTTTTAG
Protein Sequence
  • >LOC_Os06g01400.1
    MAGLAPPPLLLLPLSSSLSPAAASHHPTSPPSRTRRRRRLLSAVASADGDAPSPVSVSASAATKGPSSSSVLTFQQAIQRLQDYWASVGCAVMQCSNTEVGAGTMNPLTFLRVLGPEPWNVAYVEPSIRPDDSRYGDNPNRLQRHTQFQVILKPDPGNSQDLFLHSLSALGINVREHDIRFVEDNWESPVLGAWGLGWEVWMDGMEITQFTYFQQSGSLPLLPVSVEITYGLERILMSLQGVDHFKNIQYTKGITYGELFLENEKEMSAYYLEHANVDNIQKHFDDFEEEARSLLSLWLPIPAYDHVLKASHAFNILDSRGFVGVTERARYFGRMRSLARQCAQLWVKTRENLGYPLGTYQESNLIYPHVSEKPSRKGVVGQPRAFVLEIGTEELPPHDVIEATKQLEKSLIQILEKRRLSHGKVRSYGTPRRLAVVVENLNMKQMEEEIELRGPPVAKAFDQEGRPTKAAEGFCRKNNVLIDSLYRRTDGKTEYIYARVKESARFADEVLTEDLPTIISGISFPKSMRWNSNIVFSRPIRWIFALHGDLIVPFCFAGISSGNQSCGLRNSSLANFKVEAAELYLHTLEKAGILIDMQERKQRILHDSSILAEGVGGDIIAPDSLVQEVINLVEAPMPIIGRYDVSFLALPKDVLITVMQKHQKYFPVTSKTMGNLLPCFITVANGAIKEEVVRKGNEAVLRARYEDAKFFYKMDTQKKLSEFRDQLSSILFHERLGTMLDKMKRVENTVAEVALLLGINEKMIPAIKDAAALAMSDLATNIVTEFTSLAGIMARHYALRDGLSEQIAEALFEITLPRFSGDVFPKTDPGIVLAVTDRLDSLVGLFGAGCQPSSTNDPFGLRRISYGLVQILVENKKNFDLTKALTLVAEEQPITIDSGVIDEVVQFVTRRLEQLLVDEGINCEIVRSVLIERANCPYLASQTAIEMEAFSRTEDFPKIVEAYSRPARIIRGKEIGSALEVDASVFEKDEERALWSAYLEVADKIHPGVDIKAFADASLELLQPLEDFFTNVFVMAEDERVRNNRLALLTKVASLPKGIADLSVLPGF*