Gene Details:

• MSU gene ID: LOC_Os05g50410
• RAPdb gene ID: Os05g0580500
• Gene Symbol: OsRad21-4 OsREC8
• Genome: MSU7 ,  IRGSP-1.0
• Species: Oryza sativa

Functional Descriptions:

• These observations suggest that OsRad21-4 is essential for efficient meiosis.
• The rice OsRad21-4, an orthologue of yeast Rec8 protein, is required for efficient meiosis.
• Furthermore, fluorescent in situ hybridization experiments on OsREC8 meiocytes demonstrated that the mutation eliminated meiotic centromeric cohesion completely during prophase I and also led to the bipolar orientation of the kinetochores during the first meiotic division and accordingly resulted in premature separation of sister chromatid during meiosis I.
• OsREC8 is essential for chromatid cohesion and metaphase I monopolar orientation in rice meiosis.
• We showed that OsREC8 encodes a protein that localized to meiotic chromosomes from approximately meiotic interphase to metaphase I.
• These results suggest that OsREC8 has several essential roles in the meiotic processes.
• Finally, we found that the centromeric localization of OsSGO1 depends on OsAM1, not other meiotic proteins such as OsREC8, PAIR2, OsMER3, or ZEP1.
• Very faint OsREC8 foci persisted in the Osam1 mutant, indicating that OsAM1 is not required for the initial meiotic recruitment of OsREC8.
• Meiotic chromosome behaviors were monitored from male meiocytes of OsRad21-4-deficient lines mediated by RNAi.
• Several key meiotic proteins, including ZEP1 and OsMER3, were not loaded normally onto chromosomes in Oscom1 mutants, whereas the localization of OsREC8, PAIR2 and PAIR3 seemed to be normal.
• Moreover, OsCOM1 was loaded normally onto meiotic chromosomes in OsREC8, zep1 and Osmer3 mutants, but could not be properly loaded in Osam1, pair2 and OsSPO11-1(RNAi) plants.

Function-related keywords:

• meiosis ,  meiotic

Literature:

• OsAM1 is required for leptotene-zygotene transition in rice .  DOI:  10.1038/cr.2011.7 ;  PMID:  21221128
• MER3 is required for normal meiotic crossover formation, but not for presynaptic alignment in rice .  DOI:  10.1242/jcs.049080 ;  PMID:  19470578
• OsSGO1 maintains synaptonemal complex stabilization in addition to protecting centromeric cohesion during rice meiosis .  DOI:  10.1111/j.1365-313X.2011.04615.x ;  PMID:  21615569
• The role of OsCOM1 in homologous chromosome synapsis and recombination in rice meiosis .  DOI:  10.1111/j.1365-313X.2012.05025.x ;  PMID:  22507309
• OsREC8 is essential for chromatid cohesion and metaphase I monopolar orientation in rice meiosis .  DOI:  10.1104/pp.111.177428 ;  PMID:  21606318
• OsSPO11-1 is essential for both homologous chromosome pairing and crossover formation in rice .  DOI:  10.1007/s00412-010-0284-7 ;  PMID:  20625906
• The rice OsRad21-4, an orthologue of yeast Rec8 protein, is required for efficient meiosis .  DOI:  10.1007/s11103-005-4922-z ;  PMID:  16525890
• Rice OsBRCA2 Is Required for DNA Double-Strand Break Repair in Meiotic Cells .  DOI:  10.3389/fpls.2020.600820 ;  PMID:  33304374

Related News:

Gene Resources:

• NCBI ID: AY371049
• UniProt accessions:

Sequences: