Information report for NL1;SNFL1
Gene Details
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Functional Descriptions
- The NL1 gene encodes a GATA-type transcription factor with a single zinc finger domain, and its transcripts are detected predominantly in the bract primordia, which normally degenerate in the wild-type plants.
- To elucidate the underlying molecular mechanisms, we cloned the rice gene NECK LEAF 1 (NL1), which when mutated results in delays in flowering time, smaller panicles with overgrown bracts and abnormal UPI elongation patterns.
- Furthermore, the expression of two regulators of flowering, Hd3a and OsMADS1, was also affected in the NL1 mutant.
- Overexpression of NL1 in transgenic plants often gives rise to severe growth retardation, less vegetative phytomers and smaller leaves, suggesting that NL1 plays an important role in organ differentiation.
- On the basis of these findings, we propose that NL1 is an intrinsic factor that modulates and coordinates organogenesis through regulating the expression of PLA1 and other regulatory genes during reproductive development in rice.
- Histological analysis showed that the length of epidermal cells and number of longitudinal veins were decreased in the flag leaf of the SNFL1 mutant.
- Analysis of RT-PCR and the SNFL1 promoter by means of a GUS fusion expression assay showed that abundance of SNFL1 transcripts was higher in the culm, leaf sheath, and root.
- We conclude that SNFL1 is an important regulator of leaf development, the identification of which might have important implications for future research on GATA transcription factors.
- Map-based cloning indicated that a member of the GATA family of transcription factors is a candidate gene for SNFL1.
- Expression of the SNFL1-GFP fusion protein in rice protoplasts showed that SNFL1 was localized in nucleus.
Functional Keywords
- transcription-factor , panicle , flower , vegetative , reproductive , leaf , growth , flowering-time , root , leaf-development , map-based-cloning , nucleus
Literature and News
- NECK LEAF 1, a GATA type transcription factor, modulates organogenesis by regulating the expression of multiple regulatory genes during reproductive development in rice . DOI: 10.1038/cr.2009.36 ; PMID: 19337211
- Short and narrow flag leaf1, a GATA zinc finger domain-containing protein, regulates flag leaf size in rice (Oryza sativa) . DOI: 10.1186/s12870-018-1452-9 ; PMID: 30413183
Gene Resources
- UniProt: Q6L5E5
- EMBL: AC007858, AC098571, AC120988
- AlphaFoldDB: Q6L5E5
- EnsemblPlants: Os05t0578900-01
- Gramene: Os05t0578900-01
- KEGG: dosa:Os05g0578900
- Orthologous matrix: AFAVWPE
- InterPro: IPR000679, IPR013088
- PANTHER: PTHR46813, PTHR46813:SF1
- SUPFAM: SSF57716
- PROSITE: PS00344, PS50114
- Gene3D: 3.30.50.10
- OrthoDB: Q6L5E5
- SWISS-MODEL: Q6L5E5
- Conserved Domain Database: cd00202
- eggNOG: KOG1601
Homologs
- Panicum virgatum: Pavir.3KG214400.1.p
Sequences
cDNA Sequence
- >LOC_Os05g50270.1
GGTAGAGTGTTGTGAATAGCCAGTTCAGTTCATCAGTTGATAGGAGCCATGCTTCACCATTACTACAGCGGCGGCGCCGGCCATCATCAGGACGTCGCTGCAGCTGGTAGCCCCGGCGACATGGCTTCCTCCACCTTCTCGCTCTTCTTCCCGATGTCCAATGGGCAGTGTTGGCCGCCGTCGACGGTGGAGGAGTCCGCGGCCTACGACGACCACAGCACCGTCACCACCTCTCCTTCCTCGCCTTCGTCGTCCTCCACCGGCTCCGTCGACTGCACGCTCTCGCTCGGCACGCCGTCGTCTCGCCGCGCCGAGCCCGTCGCGGCGGCGGCGCCAGCGGCAAACCATGGGGCGCCCGTGCCGGCGCATTATCCGTCGCTGTCAGCGGCGACCGTGTCCTGGGACGCGACTGCCGAGTCGTACTATTGTGGCCAGCAGGGGAGGCCGGCCACCGGCGCCGCCAAGTGCGCCGCCGGCGCCGGGCACGACGCGCTCCTCGACCGCCGCTGCGCCAACTGCGGCACCGCGTCCACGCCGCTCTGGAGGAACGGCCCTCGCGGACCCAAGTCGCTGTGCAACGCGTGCGGGATCAGGTACAAGAAGGAGGAGCGGCGCGCGGCGGCGACGACGACGACGGCCGACGGCGCCGCCGGATGCGGCTTCATCACCGCGCAGCGTGGACGCGGGTCGACCGCGGCCAAGGCGGCGCCCGCCGTGACGACGTGCGGCGAGGAGACGTCACCGTACGTCGTCGGCGGCGGCGGCGGCGGCGGCGAGGTCGCGGACGCGGCGTATCTCGCCTGGCGGCTCAACGTCGTCCCACCGGCGGCGACGGCGACGGCGTTCTCGGTGTGGCCGGAGCGAGCTAGCCTCTACCACTACAACTAGCTATAGGCGATTAACTAGTGTAGCATTAGCGTTGGGGAGAAGGGAAGCTGCACCGTTTTTTTGGGGAACATTTTGTACGGTCGCACGCTCCCGGATTGATCGAACTGTATGCATCTGCTGTGCTGTGTTGCACGCTAGCTAGCTAGATGACTCTGATCTTGAAACGAACCGTGATCATTTCATTCATTTGGAATGAACATGTAGCTGTGCAAGGTCATCTTGCTCGGAAAATTCATAATCTTCCAATCTCTTGTGCTGC
CDS Sequence
- >LOC_Os05g50270.1
ATGCTTCACCATTACTACAGCGGCGGCGCCGGCCATCATCAGGACGTCGCTGCAGCTGGTAGCCCCGGCGACATGGCTTCCTCCACCTTCTCGCTCTTCTTCCCGATGTCCAATGGGCAGTGTTGGCCGCCGTCGACGGTGGAGGAGTCCGCGGCCTACGACGACCACAGCACCGTCACCACCTCTCCTTCCTCGCCTTCGTCGTCCTCCACCGGCTCCGTCGACTGCACGCTCTCGCTCGGCACGCCGTCGTCTCGCCGCGCCGAGCCCGTCGCGGCGGCGGCGCCAGCGGCAAACCATGGGGCGCCCGTGCCGGCGCATTATCCGTCGCTGTCAGCGGCGACCGTGTCCTGGGACGCGACTGCCGAGTCGTACTATTGTGGCCAGCAGGGGAGGCCGGCCACCGGCGCCGCCAAGTGCGCCGCCGGCGCCGGGCACGACGCGCTCCTCGACCGCCGCTGCGCCAACTGCGGCACCGCGTCCACGCCGCTCTGGAGGAACGGCCCTCGCGGACCCAAGTCGCTGTGCAACGCGTGCGGGATCAGGTACAAGAAGGAGGAGCGGCGCGCGGCGGCGACGACGACGACGGCCGACGGCGCCGCCGGATGCGGCTTCATCACCGCGCAGCGTGGACGCGGGTCGACCGCGGCCAAGGCGGCGCCCGCCGTGACGACGTGCGGCGAGGAGACGTCACCGTACGTCGTCGGCGGCGGCGGCGGCGGCGGCGAGGTCGCGGACGCGGCGTATCTCGCCTGGCGGCTCAACGTCGTCCCACCGGCGGCGACGGCGACGGCGTTCTCGGTGTGGCCGGAGCGAGCTAGCCTCTACCACTACAACTAG
Protein Sequence
- >LOC_Os05g50270.1
MLHHYYSGGAGHHQDVAAAGSPGDMASSTFSLFFPMSNGQCWPPSTVEESAAYDDHSTVTTSPSSPSSSSTGSVDCTLSLGTPSSRRAEPVAAAAPAANHGAPVPAHYPSLSAATVSWDATAESYYCGQQGRPATGAAKCAAGAGHDALLDRRCANCGTASTPLWRNGPRGPKSLCNACGIRYKKEERRAAATTTTADGAAGCGFITAQRGRGSTAAKAAPAVTTCGEETSPYVVGGGGGGGEVADAAYLAWRLNVVPPAATATAFSVWPERASLYHYN*