Gene Details:

• MSU gene ID: LOC_Os05g46480
• RAPdb gene ID: Os05g0542500
• Gene Symbol: OsLEA3 OsLEA3-1
• Genome: MSU7 ,  IRGSP-1.0
• Species: Oryza sativa

Functional Descriptions:

• Further studies demonstrated that overexpression of OsMYB48-1 could regulate the expression of some ABA biosynthesis genes (OsNCED4, OsNCED5), early signaling genes (OsPP2C68, OSRK1) and late responsive genes (RAB21, OsLEA3, RAB16C and RAB16D) under drought stress conditions.
• Overexpression of OsDSR2 could increase salt and simulated drought (polyethyleneglycol)-stress sensitivities in rice by downregulating the expression of ABA- and stress-responsive genes including OsNCED4, SNAC1, OsbZIP23, P5CS, OsLEA3 and rab16C.
• In this report, a LEA protein gene OsLEA3-1 was identified and over-expressed in rice to test the drought resistance of transgenic lines under the field conditions.
• These results indicate that transgenic rice with significantly enhanced drought resistance and without yield penalty can be generated by over-expressing OsLEA3-1 gene with appropriate promoters and following a bipartite (stress and non-stress) in-field screening protocol.
• Drought resistance pre-screening of T(1) families at anthesis stage revealed that the over-expressing families with OsLEA3-S and OsLEA3-H constructs had significantly higher relative yield (yield under drought stress treatment/yield under normal growth conditions) than the wild type under drought stress conditions, although a yield penalty existed in T(1) families under normal growth conditions.
• OsLEA3-1 is induced by drought, salt and abscisic acid (ABA), but not by cold stress.
• The promoter of OsLEA3-1 isolated from the upland rice IRAT109 exhibits strong activity under drought- and salt-stress conditions.
• Three expression constructs consisting of the full-length cDNA driven by the drought-inducible promoter of OsLEA3-1 (OsLEA3-H), the CaMV 35S promoter (OsLEA3-S), and the rice Actin1 promoter (OsLEA3-A) were transformed into the drought-sensitive japonica rice Zhonghua 11.
• Exogenous application of ABA and exposure to salt shock (150 mM NaCl) rapidly induces a de novo, abundant OsLEA3 transcript accumulation in seedling roots, whereas application of jasmonic acid (9 microM) does not induce OsLEA3 expression.
• The ZFP179 transgenic rice exhibited significantly increased tolerance to oxidative stress, the reactive oxygen species (ROS)-scavenging ability, and expression levels of a number of stress-related genes, including OsDREB2A, OsP5CS OsProT, and OsLEA3 under salt stress.
• Except for two families (transformed with OsLEA3-A), all the other families (transformed with OsLEA3-S and OsLEA3-H constructs) had higher grain yield than the wild type under drought stress in both the field and the PVC pipes conditions.
• The encoded OsLEA3 protein has previously been found to accumulate to higher levels in roots of two salt-tolerant compared to a salt-sensitive rice variety in response to abscisic acid (ABA) [Moons et al.
• The stress-induced OsLEA3 transcript gradually declined upon prolonged salt shock, as wilting-induced damage became irreversible.
• OsLEA3 expression was compared for the salt-tolerant variety Pokkali and the salt-sensitive cultivar Taichung N1.
• The results suggest that a differential regulation of OsLEA3 expression is an aspect of the varietal differences in salt stress tolerance.
• Collectively, our results indicate that the stress-responsive proteins OsNAC5 and OsNAC6 are transcriptional activators that enhance stress tolerance by upregulating the expression of stress-inducible rice genes such as OsLEA3, although the effects of these proteins on growth are different.
• A cDNA clone OsLEA3, encoding a group 3 late-embryogenesis abundant (LEA) protein was isolated from roots of rice seedlings (Oryza sativa L.
• Real-time PCR analysis revealed that some abiotic stress-related genes, such as OsLEA3, OsTPP1, Rab25, gp1 precursor, beta-gal, LOC_Os05g11910 and LOC_Os05g39250, were down-regulated in OsbZIP52 overexpression lines.

Function-related keywords:

• drought ,  drought-resistance ,  cold-stress ,  salt ,  ABA ,  ethylene ,  oxidative ,  grain ,  yield ,  grain-yield ,  jasmonic ,  salt-stress ,  growth ,  seedling ,  jasmonic-acid ,  root ,  abiotic-stress

Literature:

• A group 3 LEA cDNA of rice, responsive to abscisic acid, but not to jasmonic acid, shows variety-specific differences in salt stress response .  DOI:  10.1016/s0378-1119(97)00059-0 ;  PMID:  9218720
• bZIP transcription factor OsbZIP52/RISBZ5: a potential negative regulator of cold and drought stress response in rice .  DOI:  10.1007/s00425-011-1564-z ;  PMID:  22189955
• The abiotic stress-responsive NAC-type transcription factor OsNAC5 regulates stress-inducible genes and stress tolerance in rice .  DOI:  10.1007/s00438-010-0557-0 ;  PMID:  20632034
• Functional analysis of a novel Cys2/His2-type zinc finger protein involved in salt tolerance in rice .  DOI:  10.1093/jxb/erq120 ;  PMID:  20460361
• Overexpression of OsMYB48-1, a novel MYB-related transcription factor, enhances drought and salinity tolerance in rice .  DOI:  10.1371/journal.pone.0092913 ;  PMID:  24667379
• A R2R3-type MYB gene, OsMYB2, is involved in salt, cold, and dehydration tolerance in rice .  DOI:  10.1093/jxb/err431 ;  PMID:  22301384
• Over-expression of a LEA gene in rice improves drought resistance under the field conditions .  DOI:  10.1007/s00122-007-0538-9 ;  PMID:  17426956
• Overexpression of a new stress-repressive gene OsDSR2 encoding a protein with a DUF966 domain increases salt and simulated drought stress sensitivities and reduces ABA sensitivity in rice .  DOI:  10.1007/s00299-013-1532-0 ;  PMID:  24247850
• Histone deacetylase HDA710 controls salt tolerance by regulating ABA signaling in rice .  DOI:  10.1111/jipb.13042 ;  PMID:  33289304
• OsNAC45 is Involved in ABA Response and Salt Tolerance in Rice .  DOI:  10.1186/s12284-020-00440-1 ;  PMID:  33284415
• OsNAC45 is Involved in ABA Response and Salt Tolerance in Rice .  DOI:  10.1186/s12284-020-00440-1 ;  PMID:  33284415

Related News:

Gene Resources:

• NCBI ID: DQ789359
• UniProt accessions:

Sequences: