Information report for OsLEA3;OsLEA3-1
Gene Details
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Functional Descriptions
- Further studies demonstrated that overexpression of OsMYB48-1 could regulate the expression of some ABA biosynthesis genes (OsNCED4, OsNCED5), early signaling genes (OsPP2C68, OSRK1) and late responsive genes (RAB21, OsLEA3, RAB16C and RAB16D) under drought stress conditions.
- Overexpression of OsDSR2 could increase salt and simulated drought (polyethyleneglycol)-stress sensitivities in rice by downregulating the expression of ABA- and stress-responsive genes including OsNCED4, SNAC1, OsbZIP23, P5CS, OsLEA3 and rab16C.
- In this report, a LEA protein gene OsLEA3-1 was identified and over-expressed in rice to test the drought resistance of transgenic lines under the field conditions.
- These results indicate that transgenic rice with significantly enhanced drought resistance and without yield penalty can be generated by over-expressing OsLEA3-1 gene with appropriate promoters and following a bipartite (stress and non-stress) in-field screening protocol.
- Drought resistance pre-screening of T(1) families at anthesis stage revealed that the over-expressing families with OsLEA3-S and OsLEA3-H constructs had significantly higher relative yield (yield under drought stress treatment/yield under normal growth conditions) than the wild type under drought stress conditions, although a yield penalty existed in T(1) families under normal growth conditions.
- OsLEA3-1 is induced by drought, salt and abscisic acid (ABA), but not by cold stress.
- The promoter of OsLEA3-1 isolated from the upland rice IRAT109 exhibits strong activity under drought- and salt-stress conditions.
- Three expression constructs consisting of the full-length cDNA driven by the drought-inducible promoter of OsLEA3-1 (OsLEA3-H), the CaMV 35S promoter (OsLEA3-S), and the rice Actin1 promoter (OsLEA3-A) were transformed into the drought-sensitive japonica rice Zhonghua 11.
- Exogenous application of ABA and exposure to salt shock (150 mM NaCl) rapidly induces a de novo, abundant OsLEA3 transcript accumulation in seedling roots, whereas application of jasmonic acid (9 microM) does not induce OsLEA3 expression.
- The ZFP179 transgenic rice exhibited significantly increased tolerance to oxidative stress, the reactive oxygen species (ROS)-scavenging ability, and expression levels of a number of stress-related genes, including OsDREB2A, OsP5CS OsProT, and OsLEA3 under salt stress.
- Except for two families (transformed with OsLEA3-A), all the other families (transformed with OsLEA3-S and OsLEA3-H constructs) had higher grain yield than the wild type under drought stress in both the field and the PVC pipes conditions.
- The encoded OsLEA3 protein has previously been found to accumulate to higher levels in roots of two salt-tolerant compared to a salt-sensitive rice variety in response to abscisic acid (ABA) [Moons et al.
- The stress-induced OsLEA3 transcript gradually declined upon prolonged salt shock, as wilting-induced damage became irreversible.
- OsLEA3 expression was compared for the salt-tolerant variety Pokkali and the salt-sensitive cultivar Taichung N1.
- The results suggest that a differential regulation of OsLEA3 expression is an aspect of the varietal differences in salt stress tolerance.
- Collectively, our results indicate that the stress-responsive proteins OsNAC5 and OsNAC6 are transcriptional activators that enhance stress tolerance by upregulating the expression of stress-inducible rice genes such as OsLEA3, although the effects of these proteins on growth are different.
- A cDNA clone OsLEA3, encoding a group 3 late-embryogenesis abundant (LEA) protein was isolated from roots of rice seedlings (Oryza sativa L.
- Real-time PCR analysis revealed that some abiotic stress-related genes, such as OsLEA3, OsTPP1, Rab25, gp1 precursor, beta-gal, LOC_Os05g11910 and LOC_Os05g39250, were down-regulated in OsbZIP52 overexpression lines.
Functional Keywords
- drought , drought-resistance , cold-stress , salt , ABA , ethylene , oxidative , grain , yield , grain-yield , jasmonic , salt-stress , growth , seedling , jasmonic-acid , root , abiotic-stress
Literature and News
- A group 3 LEA cDNA of rice, responsive to abscisic acid, but not to jasmonic acid, shows variety-specific differences in salt stress response . DOI: 10.1016/s0378-1119(97)00059-0 ; PMID: 9218720
- bZIP transcription factor OsbZIP52/RISBZ5: a potential negative regulator of cold and drought stress response in rice . DOI: 10.1007/s00425-011-1564-z ; PMID: 22189955
- The abiotic stress-responsive NAC-type transcription factor OsNAC5 regulates stress-inducible genes and stress tolerance in rice . DOI: 10.1007/s00438-010-0557-0 ; PMID: 20632034
- Functional analysis of a novel Cys2/His2-type zinc finger protein involved in salt tolerance in rice . DOI: 10.1093/jxb/erq120 ; PMID: 20460361
- Overexpression of OsMYB48-1, a novel MYB-related transcription factor, enhances drought and salinity tolerance in rice . DOI: 10.1371/journal.pone.0092913 ; PMID: 24667379
- A R2R3-type MYB gene, OsMYB2, is involved in salt, cold, and dehydration tolerance in rice . DOI: 10.1093/jxb/err431 ; PMID: 22301384
- Over-expression of a LEA gene in rice improves drought resistance under the field conditions . DOI: 10.1007/s00122-007-0538-9 ; PMID: 17426956
- Overexpression of a new stress-repressive gene OsDSR2 encoding a protein with a DUF966 domain increases salt and simulated drought stress sensitivities and reduces ABA sensitivity in rice . DOI: 10.1007/s00299-013-1532-0 ; PMID: 24247850
- Histone deacetylase HDA710 controls salt tolerance by regulating ABA signaling in rice . DOI: 10.1111/jipb.13042 ; PMID: 33289304
- OsNAC45 is Involved in ABA Response and Salt Tolerance in Rice . DOI: 10.1186/s12284-020-00440-1 ; PMID: 33284415
- OsNAC45 is Involved in ABA Response and Salt Tolerance in Rice . DOI: 10.1186/s12284-020-00440-1 ; PMID: 33284415
Gene Resources
- UniProt: V5K4U5
- EMBL: KC140128
- AlphaFoldDB: V5K4U5
- EnsemblPlants: Os05t0542500-02
- Gramene: Os05t0542500-02
- KEGG: dosa:Os05g0542500
- Orthologous matrix: MDAMATQ
- PANTHER: PTHR47372, PTHR47372:SF11
- Gene3D: 1.20.120.20
- OrthoDB: V5K4U5
- SWISS-MODEL: V5K4U5
Sequences
cDNA Sequence
- >LOC_Os05g46480.2
ACAGCCAGTGCAAATCTGATCGATCAAGATAATCGAGCAAAATCCATCAGAGTTCTAACATTCGCGCGTGAATTTCGAGGTTAATTTTTGAAGCTTAGGATCAATGGCTTCCCACCAGGACCAGGCTAGCTACCGCGCCGGCGAGACCAAGGCCCACACCGAGGAGAAGGCGGGGCAGGTGATGGGGGCGAGCAAGGACAAGGCGAGCGAGGCGAAGGACAGGGCGTCGGAGGCGGCGGGGCACGCCGCCGGCAAGGGGCAGGATACCAAGGAGGCGACGAAGGAGAAGGCGCAGGCGGCGAAGGAGAGGGCGTCGGAGACGGCGCAGGCGGCGAAGGACAAGACCTCCAGCACGTCGCAGGCGGCGAGGGACAAAGCCGCCGAGAGCAAGGACCAGACCGGCGGCTTCCTCGGCGAGAAGACCGAGCAGGCCAAGCAGAAGGCCGCCGAGACCGCTGGCGCCGCCAAGCAGAAGACCGCCGAGACGGCGCAGTACACCAAGGACTCTGCCATCGCCGGCAAGGACAAGACCGGCAGCGTCCTCCAACAGGCGAGTGAGCAGGTGAAGAGCACGGTGGTCGGCGCCAAGGACGCGGTGATGAGCACGCTGGGGATGACCGAAGACGAGGCCGGCACCGACGACGGCGCCAACAAGGACACCTCTGCCACCGCCGCCGCCACGGAGACGACGGCGAGGGATCACTAGACGCCGTGAATGATTTCCCTTTGGGTCTATTTATTGGTGGTCGTCGCAGGGGAGGATTTCTGACGGGTGTGGGTGATGTGTACTGATGATGTTCAGTTGTTTTCGTCAGAGTTTCTCGTCTGTGTTCTGTTTATTATGGCGTAACAATAATAAAAGTTTGGGCCTAAAGCCCGCATTTGTGGTTTATCATTTCGATGTGTTTACATTC - >LOC_Os05g46480.3
ACAGCCAGTGCAAATCTGATCGATCAAGATAATCGAGCAAAATCCATCAGAGTTCTAACATTCGCGCGTGAATTTCGAGGTTAATTTTTGAAGCTTAGGATCAATGGCTTCCCACCAGGACCAGGCTAGCTACCGCGCCGGCGAGACCAAGGCCCACACCGAGGAGAAGGCGGGGCAGGTGATGGGGGCGAGCAAGGACAAGGCGAGCGAGGCGAAGGACAGGGCGTCGGAGGCGGCGGGGCACGCCGCCGGCAAGGGGCAGGATACCAAGGAGGCGACGAAGGAGAAGGCGCAGGCGGCGAAGGAGAGGGCGTCGGAGACGGCGCAGGCGGCGAAGGACAAGACCTCCAGCACGTCGCAGGCGGCGAGGGACAAAGCCGCCGAGAGCAAGGACCAGACCGGCGGCTTCCTCGGCGAGAAGACCGAGCAGGCCAAGCAGAAGGCCGCCGAGACCGCTGGCGCCGCCAAGCAGAAGACCGCCGAGACGGCGCAGTACACCAAGGACTCTGCCATCGCCGGCAAGGACAAGACCGGCAGCGTCCTCCAACAGGCGAGTGAGCAGGTGAAGAGCACGGTGGTCGGCGCCAAGGACGCGGTGATGAGCACGCTGGGGATGACCGAAGACGAGGCCGGCACCGACGACGGCGCCAACAAGGACACCTCTGCCACCGCCGCCGCCACGGAGACGACGGCGAGGGATCACTAGACGCCGTGAATGATTTCCCTTTGGGTCTATTTATTGGTGGTCGTCGCAGGGGAGGATTTCTGACGGGTGTGGGTGATGTGTACTGATGATGTTCAGTTGTTTTCGTCAGAGTTTCTCGTCTGTGTTCTGTTTATTATGGCGTAACAATAATAAAAGTTTGGGCCTAAAGCCCGCATTTGTGGTTTATCATTTCGATGTGTTTACATTC - >LOC_Os05g46480.1
ACAGCCAGTGCAAATCTGATCGATCAAGATAATCGAGCAAAATCCATCAGAGTTCTAACATTCGCGCGTGAATTTCGAGGTTAATTTTTGAAGCTTAGGATCAATGGCTTCCCACCAGGACCAGGCTAGCTACCGCGCCGGCGAGACCAAGGCCCACACCGAGGAGAAGGCGGGGCAGGTGATGGGGGCGAGCAAGGACAAGGCGAGCGAGGCGAAGGACAGGGCGTCGGAGGCGGCGGGGCACGCCGCCGGCAAGGGGCAGGATACCAAGGAGGCGACGAAGGAGAAGGCGCAGGCGGCGAAGGAGAGGGCGTCGGAGACGGCGCAGGCGGCGAAGGACAAGACCTCCAGCACGTCGCAGGCGGCGAGGGACAAAGCCGCCGAGAGCAAGGACCAGACCGGCGGCTTCCTCGGCGAGAAGACCGAGCAGGCCAAGCAGAAGGCCGCCGAGACCGCTGGCGCCGCCAAGCAGAAGACCGCCGAGACGGCGCAGTACACCAAGGACTCTGCCATCGCCGGCAAGGACAAGACCGGCAGCGTCCTCCAACAGGCGAGTGAGCAGGTGAAGAGCACGGTGGTCGGCGCCAAGGACGCGGTGATGAGCACGCTGGGGATGACCGAAGACGAGGCCGGCACCGACGACGGCGCCAACAAGGACACCTCTGCCACCGCCGCCGCCACGGAGACGACGGCGAGGGATCACTAGACGCCGTGAATGATTTCCCTTTGGGTCTATTTATTGGTGGTCGTCGCAGGGGAGGATTTCTGACGGGTGTGGGTGATGTGTACTGATGATGTTCAGTTGTTTTCGTCAGAGTTTCTCGTCTGTGTTCTGTTTATTATGGCGTAACAATAATAAAAGTTTGGGCCTAAAGCCCGCATTTGTGGTTTATCATTTCGATGTGTTTACATTC - >LOC_Os05g46480.4
ACAGCCAGTGCAAATCTGATCGATCAAGATAATCGAGCAAAATCCATCAGAGTTCTAACATTCGCGCGTGAATTTCGAGGTTAATTTTTGAAGCTTAGGATCAATGGCTTCCCACCAGGACCAGGCTAGCTACCGCGCCGGCGAGACCAAGGCCCACACCGAGGAGAAGGCGGGGCAGGTGATGGGGGCGAGCAAGGACAAGGCGAGCGAGGCGAAGGACAGGGCGTCGGAGGCGGCGGGGCACGCCGCCGGCAAGGGGCAGGATACCAAGGAGGCGACGAAGGAGAAGGCGCAGGCGGCGAAGGAGAGGGCGTCGGAGACGGCGCAGGCGGCGAAGGACAAGACCTCCAGCACGTCGCAGGCGGCGAGGGACAAAGCCGCCGAGAGCAAGGACCAGACCGGCGGCTTCCTCGGCGAGAAGACCGAGCAGGCCAAGCAGAAGGCCGCCGAGACCGCTGGCGCCGCCAAGCAGAAGACCGCCGAGACGGCGCAGTACACCAAGGACTCTGCCATCGCCGGCAAGGACAAGACCGGCAGCGTCCTCCAACAGGCGAGTGAGCAGGTGAAGAGCACGGTGGTCGGCGCCAAGGACGCGGTGATGAGCACGCTGGGGATGACCGAAGACGAGGCCGGCACCGACGACGGCGCCAACAAGGACACCTCTGCCACCGCCGCCGCCACGGAGACGACGGCGAGGGATCACTAGACGCCGTGAATGATTTCCCTTTGGGTCTATTTATTGGTGGTCGTCGCAGGGGAGGATTTCTGACGGGTGTGGGTGATGTGTACTGATGATGTTCAGTTGTTTTCGTCAGAGTTTCTCGTCTGTGTTCTGTTTATTATGGCGTAACAATAATAAAAGTTTGGGCCTAAAGCCCGCATTTGTGGTTTATCATTTCGATGTGTTTACATTC
CDS Sequence
- >LOC_Os05g46480.2
ATGGCTTCCCACCAGGACCAGGCTAGCTACCGCGCCGGCGAGACCAAGGCCCACACCGAGGAGAAGGCGGGGCAGGTGATGGGGGCGAGCAAGGACAAGGCGAGCGAGGCGAAGGACAGGGCGTCGGAGGCGGCGGGGCACGCCGCCGGCAAGGGGCAGGATACCAAGGAGGCGACGAAGGAGAAGGCGCAGGCGGCGAAGGAGAGGGCGTCGGAGACGGCGCAGGCGGCGAAGGACAAGACCTCCAGCACGTCGCAGGCGGCGAGGGACAAAGCCGCCGAGAGCAAGGACCAGACCGGCGGCTTCCTCGGCGAGAAGACCGAGCAGGCCAAGCAGAAGGCCGCCGAGACCGCTGGCGCCGCCAAGCAGAAGACCGCCGAGACGGCGCAGTACACCAAGGACTCTGCCATCGCCGGCAAGGACAAGACCGGCAGCGTCCTCCAACAGGCGAGTGAGCAGGTGAAGAGCACGGTGGTCGGCGCCAAGGACGCGGTGATGAGCACGCTGGGGATGACCGAAGACGAGGCCGGCACCGACGACGGCGCCAACAAGGACACCTCTGCCACCGCCGCCGCCACGGAGACGACGGCGAGGGATCACTAG - >LOC_Os05g46480.3
ATGGCTTCCCACCAGGACCAGGCTAGCTACCGCGCCGGCGAGACCAAGGCCCACACCGAGGAGAAGGCGGGGCAGGTGATGGGGGCGAGCAAGGACAAGGCGAGCGAGGCGAAGGACAGGGCGTCGGAGGCGGCGGGGCACGCCGCCGGCAAGGGGCAGGATACCAAGGAGGCGACGAAGGAGAAGGCGCAGGCGGCGAAGGAGAGGGCGTCGGAGACGGCGCAGGCGGCGAAGGACAAGACCTCCAGCACGTCGCAGGCGGCGAGGGACAAAGCCGCCGAGAGCAAGGACCAGACCGGCGGCTTCCTCGGCGAGAAGACCGAGCAGGCCAAGCAGAAGGCCGCCGAGACCGCTGGCGCCGCCAAGCAGAAGACCGCCGAGACGGCGCAGTACACCAAGGACTCTGCCATCGCCGGCAAGGACAAGACCGGCAGCGTCCTCCAACAGGCGAGTGAGCAGGTGAAGAGCACGGTGGTCGGCGCCAAGGACGCGGTGATGAGCACGCTGGGGATGACCGAAGACGAGGCCGGCACCGACGACGGCGCCAACAAGGACACCTCTGCCACCGCCGCCGCCACGGAGACGACGGCGAGGGATCACTAG - >LOC_Os05g46480.1
ATGGCTTCCCACCAGGACCAGGCTAGCTACCGCGCCGGCGAGACCAAGGCCCACACCGAGGAGAAGGCGGGGCAGGTGATGGGGGCGAGCAAGGACAAGGCGAGCGAGGCGAAGGACAGGGCGTCGGAGGCGGCGGGGCACGCCGCCGGCAAGGGGCAGGATACCAAGGAGGCGACGAAGGAGAAGGCGCAGGCGGCGAAGGAGAGGGCGTCGGAGACGGCGCAGGCGGCGAAGGACAAGACCTCCAGCACGTCGCAGGCGGCGAGGGACAAAGCCGCCGAGAGCAAGGACCAGACCGGCGGCTTCCTCGGCGAGAAGACCGAGCAGGCCAAGCAGAAGGCCGCCGAGACCGCTGGCGCCGCCAAGCAGAAGACCGCCGAGACGGCGCAGTACACCAAGGACTCTGCCATCGCCGGCAAGGACAAGACCGGCAGCGTCCTCCAACAGGCGAGTGAGCAGGTGAAGAGCACGGTGGTCGGCGCCAAGGACGCGGTGATGAGCACGCTGGGGATGACCGAAGACGAGGCCGGCACCGACGACGGCGCCAACAAGGACACCTCTGCCACCGCCGCCGCCACGGAGACGACGGCGAGGGATCACTAG - >LOC_Os05g46480.4
ATGGCTTCCCACCAGGACCAGGCTAGCTACCGCGCCGGCGAGACCAAGGCCCACACCGAGGAGAAGGCGGGGCAGGTGATGGGGGCGAGCAAGGACAAGGCGAGCGAGGCGAAGGACAGGGCGTCGGAGGCGGCGGGGCACGCCGCCGGCAAGGGGCAGGATACCAAGGAGGCGACGAAGGAGAAGGCGCAGGCGGCGAAGGAGAGGGCGTCGGAGACGGCGCAGGCGGCGAAGGACAAGACCTCCAGCACGTCGCAGGCGGCGAGGGACAAAGCCGCCGAGAGCAAGGACCAGACCGGCGGCTTCCTCGGCGAGAAGACCGAGCAGGCCAAGCAGAAGGCCGCCGAGACCGCTGGCGCCGCCAAGCAGAAGACCGCCGAGACGGCGCAGTACACCAAGGACTCTGCCATCGCCGGCAAGGACAAGACCGGCAGCGTCCTCCAACAGGCGAGTGAGCAGGTGAAGAGCACGGTGGTCGGCGCCAAGGACGCGGTGATGAGCACGCTGGGGATGACCGAAGACGAGGCCGGCACCGACGACGGCGCCAACAAGGACACCTCTGCCACCGCCGCCGCCACGGAGACGACGGCGAGGGATCACTAG
Protein Sequence
- >LOC_Os05g46480.2
MASHQDQASYRAGETKAHTEEKAGQVMGASKDKASEAKDRASEAAGHAAGKGQDTKEATKEKAQAAKERASETAQAAKDKTSSTSQAARDKAAESKDQTGGFLGEKTEQAKQKAAETAGAAKQKTAETAQYTKDSAIAGKDKTGSVLQQASEQVKSTVVGAKDAVMSTLGMTEDEAGTDDGANKDTSATAAATETTARDH* - >LOC_Os05g46480.3
MASHQDQASYRAGETKAHTEEKAGQVMGASKDKASEAKDRASEAAGHAAGKGQDTKEATKEKAQAAKERASETAQAAKDKTSSTSQAARDKAAESKDQTGGFLGEKTEQAKQKAAETAGAAKQKTAETAQYTKDSAIAGKDKTGSVLQQASEQVKSTVVGAKDAVMSTLGMTEDEAGTDDGANKDTSATAAATETTARDH* - >LOC_Os05g46480.1
MASHQDQASYRAGETKAHTEEKAGQVMGASKDKASEAKDRASEAAGHAAGKGQDTKEATKEKAQAAKERASETAQAAKDKTSSTSQAARDKAAESKDQTGGFLGEKTEQAKQKAAETAGAAKQKTAETAQYTKDSAIAGKDKTGSVLQQASEQVKSTVVGAKDAVMSTLGMTEDEAGTDDGANKDTSATAAATETTARDH* - >LOC_Os05g46480.4
MASHQDQASYRAGETKAHTEEKAGQVMGASKDKASEAKDRASEAAGHAAGKGQDTKEATKEKAQAAKERASETAQAAKDKTSSTSQAARDKAAESKDQTGGFLGEKTEQAKQKAAETAGAAKQKTAETAQYTKDSAIAGKDKTGSVLQQASEQVKSTVVGAKDAVMSTLGMTEDEAGTDDGANKDTSATAAATETTARDH*