Information report for OsABIL3;OsPP2C50
Gene Details
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Functional Descriptions
- Transgenic Arabidopsis plants overexpressing OsPP2C50 and OsPP2C50FM changed ABA sensitivity, validating the in vitro results.
- The effects of the second and forth residues on the interaction of OsPP2C50 with several OsPYL/RCAR proteins were investigated by systematic mutagenesis.
- The water-mediated interaction between the wedging Trp-259 of OsPP2C50 and pyrabactin is lost, undermining the structural integrity of the ABA receptor:co-receptor.
- OsPP2C50 and OsPP2C53 is major negative regulators of ABA signaling regarding stomata closing in rice.
- We identified that only OsPP2C50 and OsPP2C53 among 9 OsPP2CAs might be related with stomatal closure/opening signaling based on guard cell specific expression and subcellular localization.
- Transgenic rice overexpressing OsPP2C50 and OsPP2C53 showed significantly higher water loss than control.
Functional Keywords
- ABA , R-protein , stomatal , stomata , water-loss
Literature and News
- ABA Regulates Subcellular Redistribution of OsABI-LIKE2, a Negative Regulator in ABA Signaling, to Control Root Architecture and Drought Resistance in Oryza sativa . DOI: 10.1093/pcp/pcv154 ; PMID: 26491145
- Modulation of ABA Signaling by Altering VxGΦL Motif of PP2Cs in Oryza sativa . DOI: 10.1016/j.molp.2017.08.003 ; PMID: 28827170
- Structural determinants for pyrabactin recognition in ABA receptors in Oryza sativa . DOI: 10.1007/s11103-019-00862-6 ; PMID: 30941543
- Two Clade A Phosphatase 2Cs Expressed in Guard Cells Physically Interact With Abscisic Acid Signaling Components to Induce Stomatal Closure in Rice . DOI: 10.1186/s12284-019-0297-7 ; PMID: 31134357
Gene Resources
- UniProt: Q6L5H6
- EMBL: AC097112, AC119291, AP008211
- AlphaFoldDB: Q6L5H6
- EnsemblPlants: Os05t0537400-01
- Gramene: Os05t0537400-01
- KEGG: dosa:Os05g0537400
- Orthologous matrix: HIDPCAR
- InterPro: IPR000222, IPR001932, IPR015655
Sequences
cDNA Sequence
- >LOC_Os05g46040.1
ATGGCGGCGGCGGCGGCGGCGGCGGCGATATGTGGGGAGGATGAGACGGCGGCGCGGGTGGGGTGCACGGGGGAATGGGCGGGCGGGATCGAGAGGGTGGATCTTGGGGAGAGGAAGGAGGCGGTGGCGGCGGCGGGGGCGGGGAAGAGGAGCGTCTACCTGATGGACTGCGCGCCGGTGTGGGGCTGCGCGTCGACGCGCGGCCGCAGCGCGGAGATGGAGGACGCGAGCGCCGCCGTGCCGCGGTTCGCGGACGTGCCGGTGCGGCTGCTCGCCAGCCGGCGCGACCTCGACGCGCTGGGCCTCGACGCCGACGCGCTCCGCCTGCCGGCGCACCTCTTCGGCGTGTTCGACGGCCACGGCGGCGCCGAGGTGGCGAACTACTGCCGTGAAAGGATCCACGTCGTCTTGAGCGAGGAGCTGAAGCGACTTGGCAAGAATTTGGGGGAGATGGGCGAGGTGGACATGAAAGAGCACTGGGATGATGTGTTCACGAAATGTTTCCAGAGAGTGGACGATGAGGTTTCAGGGAGAGTGACCAGGGTTGTCAATGGTGGCGGTGAGGTCCGGTCAGAACCGGTGACCGCAGAGAACGTCGGCTCGACGGCGGTCGTTGCGCTTGTCTGCTCATCTCATGTGGTGGTTGCCAACTGTGGAGATTCGCGCATCGTGCTCTGCCGCGGGAAGGAGCCCGTAGCCTTGTCAATTGATCACAAGCCTGACAGGAAGGATGAGCGGGCAAGGATTGAAGCCCAGGGAGGCAAGGTCATCCAATGGAATGGTTACCGGGTGTCCGGTATACTTGCTATGTCCCGATCAATCGGTGATCGCTATCTGAAACCATTTGTCATTCCAAAACCGGAAGTTATGGTCGTTCCACGGGCGAAGGATGATGACTGTCTTATTCTAGCAAGCGATGGGCTGTGGGATGTTGTGTCAAATGAAGAGGCATGCAAAGTCGCACGCCGACAGATCCTTCTGTGGCACAAGAACAATGGCGCTGCATCACCATTGTCTGATGAGGGTGAAGGATCCACCGACCCTGCTGCCCAAGCAGCTGCCGATTATCTGATGAGACTCGCTCTGAAGAAAGGCAGCGAGGATAACATCACTGTCATTGTTGTCGACTTGAAACCGCGAAAGAAACTCAAGAACATTTCATAACAGGGCCTGGGTTCATCTTGAGTAGCTGACCATGATGTGTGTCAAAGAGTTTCATACAACTAGTCGGTCTTCCTCACGGGACTTGACCCACTGTTTTTTTTGCTGGGCTTCAAGAAATTGTACATGTATATGCTAGATGTATCTTCCATCTAGGGATAAATATCTACCTACCTCCATTCTATCCCGTCAGCTTCAGTTTTCAGCCCTGGCAAGAAGTATCCCTTGTACAATAATTGAACTTGGAAAAAGCTAAGTGGCTGAGATGACCAGTAAAATTAACATAATTGTGATCACCTTTCCTGAA - >LOC_Os05g46040.2
ATGGCGGCGGCGGCGGCGGCGGCGGCGATATGTGGGGAGGATGAGACGGCGGCGCGGGTGGGGTGCACGGGGGAATGGGCGGGCGGGATCGAGAGGGTGGATCTTGGGGAGAGGAAGGAGGCGGTGGCGGCGGCGGGGGCGGGGAAGAGGAGCGTCTACCTGATGGACTGCGCGCCGGTGTGGGGCTGCGCGTCGACGCGCGGCCGCAGCGCGGAGATGGAGGACGCGAGCGCCGCCGTGCCGCGGTTCGCGGACGTGCCGGTGCGGCTGCTCGCCAGCCGGCGCGACCTCGACGCGCTGGGCCTCGACGCCGACGCGCTCCGCCTGCCGGCGCACCTCTTCGGCGTGTTCGACGGCCACGGCGGCGCCGAGGTGGCGAACTACTGCCGTGAAAGGATCCACGTCGTCTTGAGCGAGGAGCTGAAGCGACTTGGCAAGAATTTGGGGGAGATGGGCGAGGTGGACATGAAAGAGCACTGGGATGATGTGTTCACGAAATGTTTCCAGAGAGTGGACGATGAGGTTTCAGGGAGAGTGACCAGGGTTGTCAATGGTGGCGGTGAGGTCCGGTCAGAACCGGTGACCGCAGAGAACGTCGGCTCGACGGCGGTCGTTGCGCTTGTCTGCTCATCTCATGTGGTGGTTGCCAACTGTGGAGATTCGCGCATCGTGCTCTGCCGCGGGAAGGAGCCCGTAGCCTTGTCAATTGATCACAAGCCTGACAGGAAGGATGAGCGGGCAAGGATTGAAGCCCAGGGAGGCAAGGTCATCCAATGGAATGGTTACCGGGTGTCCGGTATACTTGCTATGTCCCGATCAATCGGTGATCGCTATCTGAAACCATTTGTCATTCCAAAACCGGAAGTTATGGTCGTTCCACGGGCGAAGGATGATGACTGTCTTATTCTAGCAAGCGATGGGCTGTGGGATGTTGTGTCAAATGAAGAGGCATGCAAAGTCGCACGCCGACAGATCCTTCTGTGGCACAAGAACAATGGCGCTGCATCACCATTGTCTGATGAGGGTGAAGGATCCACCGACCCTGCTGCCCAAGCAGCTGCCGATTATCTGATGAGACTCGCTCTGAAGAAAGGCAGCGAGGATAACATCACTGTCATTGTTGTCGACTTGAAACCGCGAAAGAAACTCAAGAACATTTCATAACAGGGCCTGGGTTCATCTTGAGTAGCTGACCATGATGTGTGTCAAAGAGTTTCATACAACTAGTCGGTCTTCCTCACGGGACTTGACCCACTGTTTTTTTTGCTGGGCTTCAAGAAATTGTACATGTATATGCTAGATGTATCTTCCATCTAGGGATAAATATCTACCTACCTCCATTCTATCCCGTCAGCTTCAGTTTTCAGCCCTGGCAAGAAGTATCCCTTGTACAATAATTGAACTTGGAAAAAGCTAAGTGGCTGAGATGACCAGTAAAATTAACATAATTGTGATCACCTTTCCTGAA
CDS Sequence
- >LOC_Os05g46040.1
ATGGCGGCGGCGGCGGCGGCGGCGGCGATATGTGGGGAGGATGAGACGGCGGCGCGGGTGGGGTGCACGGGGGAATGGGCGGGCGGGATCGAGAGGGTGGATCTTGGGGAGAGGAAGGAGGCGGTGGCGGCGGCGGGGGCGGGGAAGAGGAGCGTCTACCTGATGGACTGCGCGCCGGTGTGGGGCTGCGCGTCGACGCGCGGCCGCAGCGCGGAGATGGAGGACGCGAGCGCCGCCGTGCCGCGGTTCGCGGACGTGCCGGTGCGGCTGCTCGCCAGCCGGCGCGACCTCGACGCGCTGGGCCTCGACGCCGACGCGCTCCGCCTGCCGGCGCACCTCTTCGGCGTGTTCGACGGCCACGGCGGCGCCGAGGTGGCGAACTACTGCCGTGAAAGGATCCACGTCGTCTTGAGCGAGGAGCTGAAGCGACTTGGCAAGAATTTGGGGGAGATGGGCGAGGTGGACATGAAAGAGCACTGGGATGATGTGTTCACGAAATGTTTCCAGAGAGTGGACGATGAGGTTTCAGGGAGAGTGACCAGGGTTGTCAATGGTGGCGGTGAGGTCCGGTCAGAACCGGTGACCGCAGAGAACGTCGGCTCGACGGCGGTCGTTGCGCTTGTCTGCTCATCTCATGTGGTGGTTGCCAACTGTGGAGATTCGCGCATCGTGCTCTGCCGCGGGAAGGAGCCCGTAGCCTTGTCAATTGATCACAAGCCTGACAGGAAGGATGAGCGGGCAAGGATTGAAGCCCAGGGAGGCAAGGTCATCCAATGGAATGGTTACCGGGTGTCCGGTATACTTGCTATGTCCCGATCAATCGGTGATCGCTATCTGAAACCATTTGTCATTCCAAAACCGGAAGTTATGGTCGTTCCACGGGCGAAGGATGATGACTGTCTTATTCTAGCAAGCGATGGGCTGTGGGATGTTGTGTCAAATGAAGAGGCATGCAAAGTCGCACGCCGACAGATCCTTCTGTGGCACAAGAACAATGGCGCTGCATCACCATTGTCTGATGAGGGTGAAGGATCCACCGACCCTGCTGCCCAAGCAGCTGCCGATTATCTGATGAGACTCGCTCTGAAGAAAGGCAGCGAGGATAACATCACTGTCATTGTTGTCGACTTGAAACCGCGAAAGAAACTCAAGAACATTTCATAA - >LOC_Os05g46040.2
ATGGCGGCGGCGGCGGCGGCGGCGGCGATATGTGGGGAGGATGAGACGGCGGCGCGGGTGGGGTGCACGGGGGAATGGGCGGGCGGGATCGAGAGGGTGGATCTTGGGGAGAGGAAGGAGGCGGTGGCGGCGGCGGGGGCGGGGAAGAGGAGCGTCTACCTGATGGACTGCGCGCCGGTGTGGGGCTGCGCGTCGACGCGCGGCCGCAGCGCGGAGATGGAGGACGCGAGCGCCGCCGTGCCGCGGTTCGCGGACGTGCCGGTGCGGCTGCTCGCCAGCCGGCGCGACCTCGACGCGCTGGGCCTCGACGCCGACGCGCTCCGCCTGCCGGCGCACCTCTTCGGCGTGTTCGACGGCCACGGCGGCGCCGAGGTGGCGAACTACTGCCGTGAAAGGATCCACGTCGTCTTGAGCGAGGAGCTGAAGCGACTTGGCAAGAATTTGGGGGAGATGGGCGAGGTGGACATGAAAGAGCACTGGGATGATGTGTTCACGAAATGTTTCCAGAGAGTGGACGATGAGGTTTCAGGGAGAGTGACCAGGGTTGTCAATGGTGGCGGTGAGGTCCGGTCAGAACCGGTGACCGCAGAGAACGTCGGCTCGACGGCGGTCGTTGCGCTTGTCTGCTCATCTCATGTGGTGGTTGCCAACTGTGGAGATTCGCGCATCGTGCTCTGCCGCGGGAAGGAGCCCGTAGCCTTGTCAATTGATCACAAGCCTGACAGGAAGGATGAGCGGGCAAGGATTGAAGCCCAGGGAGGCAAGGTCATCCAATGGAATGGTTACCGGGTGTCCGGTATACTTGCTATGTCCCGATCAATCGGTGATCGCTATCTGAAACCATTTGTCATTCCAAAACCGGAAGTTATGGTCGTTCCACGGGCGAAGGATGATGACTGTCTTATTCTAGCAAGCGATGGGCTGTGGGATGTTGTGTCAAATGAAGAGGCATGCAAAGTCGCACGCCGACAGATCCTTCTGTGGCACAAGAACAATGGCGCTGCATCACCATTGTCTGATGAGGGTGAAGGATCCACCGACCCTGCTGCCCAAGCAGCTGCCGATTATCTGATGAGACTCGCTCTGAAGAAAGGCAGCGAGGATAACATCACTGTCATTGTTGTCGACTTGAAACCGCGAAAGAAACTCAAGAACATTTCATAA
Protein Sequence
- >LOC_Os05g46040.1
MAAAAAAAAICGEDETAARVGCTGEWAGGIERVDLGERKEAVAAAGAGKRSVYLMDCAPVWGCASTRGRSAEMEDASAAVPRFADVPVRLLASRRDLDALGLDADALRLPAHLFGVFDGHGGAEVANYCRERIHVVLSEELKRLGKNLGEMGEVDMKEHWDDVFTKCFQRVDDEVSGRVTRVVNGGGEVRSEPVTAENVGSTAVVALVCSSHVVVANCGDSRIVLCRGKEPVALSIDHKPDRKDERARIEAQGGKVIQWNGYRVSGILAMSRSIGDRYLKPFVIPKPEVMVVPRAKDDDCLILASDGLWDVVSNEEACKVARRQILLWHKNNGAASPLSDEGEGSTDPAAQAAADYLMRLALKKGSEDNITVIVVDLKPRKKLKNIS* - >LOC_Os05g46040.2
MAAAAAAAAICGEDETAARVGCTGEWAGGIERVDLGERKEAVAAAGAGKRSVYLMDCAPVWGCASTRGRSAEMEDASAAVPRFADVPVRLLASRRDLDALGLDADALRLPAHLFGVFDGHGGAEVANYCRERIHVVLSEELKRLGKNLGEMGEVDMKEHWDDVFTKCFQRVDDEVSGRVTRVVNGGGEVRSEPVTAENVGSTAVVALVCSSHVVVANCGDSRIVLCRGKEPVALSIDHKPDRKDERARIEAQGGKVIQWNGYRVSGILAMSRSIGDRYLKPFVIPKPEVMVVPRAKDDDCLILASDGLWDVVSNEEACKVARRQILLWHKNNGAASPLSDEGEGSTDPAAQAAADYLMRLALKKGSEDNITVIVVDLKPRKKLKNIS*