Gene Details:

Functional Descriptions:

  • Transgenic Arabidopsis plants overexpressing OsPP2C50 and OsPP2C50FM changed ABA sensitivity, validating the in vitro results.
  • The effects of the second and forth residues on the interaction of OsPP2C50 with several OsPYL/RCAR proteins were investigated by systematic mutagenesis.
  • The water-mediated interaction between the wedging Trp-259 of OsPP2C50 and pyrabactin is lost, undermining the structural integrity of the ABA receptor:co-receptor.
  • OsPP2C50 and OsPP2C53 is major negative regulators of ABA signaling regarding stomata closing in rice.
  • We identified that only OsPP2C50 and OsPP2C53 among 9 OsPP2CAs might be related with stomatal closure/opening signaling based on guard cell specific expression and subcellular localization.
  • Transgenic rice overexpressing OsPP2C50 and OsPP2C53 showed significantly higher water loss than control.

Literature:

Gene Resources:

  • NCBI ID:
  • UniProt accessions:

Sequences:

cDNA Sequence
  • >LOC_Os05g46040.1
    ATGGCGGCGGCGGCGGCGGCGGCGGCGATATGTGGGGAGGATGAGACGGCGGCGCGGGTGGGGTGCACGGGGGAATGGGCGGGCGGGATCGAGAGGGTGGATCTTGGGGAGAGGAAGGAGGCGGTGGCGGCGGCGGGGGCGGGGAAGAGGAGCGTCTACCTGATGGACTGCGCGCCGGTGTGGGGCTGCGCGTCGACGCGCGGCCGCAGCGCGGAGATGGAGGACGCGAGCGCCGCCGTGCCGCGGTTCGCGGACGTGCCGGTGCGGCTGCTCGCCAGCCGGCGCGACCTCGACGCGCTGGGCCTCGACGCCGACGCGCTCCGCCTGCCGGCGCACCTCTTCGGCGTGTTCGACGGCCACGGCGGCGCCGAGGTGGCGAACTACTGCCGTGAAAGGATCCACGTCGTCTTGAGCGAGGAGCTGAAGCGACTTGGCAAGAATTTGGGGGAGATGGGCGAGGTGGACATGAAAGAGCACTGGGATGATGTGTTCACGAAATGTTTCCAGAGAGTGGACGATGAGGTTTCAGGGAGAGTGACCAGGGTTGTCAATGGTGGCGGTGAGGTCCGGTCAGAACCGGTGACCGCAGAGAACGTCGGCTCGACGGCGGTCGTTGCGCTTGTCTGCTCATCTCATGTGGTGGTTGCCAACTGTGGAGATTCGCGCATCGTGCTCTGCCGCGGGAAGGAGCCCGTAGCCTTGTCAATTGATCACAAGCCTGACAGGAAGGATGAGCGGGCAAGGATTGAAGCCCAGGGAGGCAAGGTCATCCAATGGAATGGTTACCGGGTGTCCGGTATACTTGCTATGTCCCGATCAATCGGTGATCGCTATCTGAAACCATTTGTCATTCCAAAACCGGAAGTTATGGTCGTTCCACGGGCGAAGGATGATGACTGTCTTATTCTAGCAAGCGATGGGCTGTGGGATGTTGTGTCAAATGAAGAGGCATGCAAAGTCGCACGCCGACAGATCCTTCTGTGGCACAAGAACAATGGCGCTGCATCACCATTGTCTGATGAGGGTGAAGGATCCACCGACCCTGCTGCCCAAGCAGCTGCCGATTATCTGATGAGACTCGCTCTGAAGAAAGGCAGCGAGGATAACATCACTGTCATTGTTGTCGACTTGAAACCGCGAAAGAAACTCAAGAACATTTCATAACAGGGCCTGGGTTCATCTTGAGTAGCTGACCATGATGTGTGTCAAAGAGTTTCATACAACTAGTCGGTCTTCCTCACGGGACTTGACCCACTGTTTTTTTTGCTGGGCTTCAAGAAATTGTACATGTATATGCTAGATGTATCTTCCATCTAGGGATAAATATCTACCTACCTCCATTCTATCCCGTCAGCTTCAGTTTTCAGCCCTGGCAAGAAGTATCCCTTGTACAATAATTGAACTTGGAAAAAGCTAAGTGGCTGAGATGACCAGTAAAATTAACATAATTGTGATCACCTTTCCTGAA
  • >LOC_Os05g46040.2
    ATGGCGGCGGCGGCGGCGGCGGCGGCGATATGTGGGGAGGATGAGACGGCGGCGCGGGTGGGGTGCACGGGGGAATGGGCGGGCGGGATCGAGAGGGTGGATCTTGGGGAGAGGAAGGAGGCGGTGGCGGCGGCGGGGGCGGGGAAGAGGAGCGTCTACCTGATGGACTGCGCGCCGGTGTGGGGCTGCGCGTCGACGCGCGGCCGCAGCGCGGAGATGGAGGACGCGAGCGCCGCCGTGCCGCGGTTCGCGGACGTGCCGGTGCGGCTGCTCGCCAGCCGGCGCGACCTCGACGCGCTGGGCCTCGACGCCGACGCGCTCCGCCTGCCGGCGCACCTCTTCGGCGTGTTCGACGGCCACGGCGGCGCCGAGGTGGCGAACTACTGCCGTGAAAGGATCCACGTCGTCTTGAGCGAGGAGCTGAAGCGACTTGGCAAGAATTTGGGGGAGATGGGCGAGGTGGACATGAAAGAGCACTGGGATGATGTGTTCACGAAATGTTTCCAGAGAGTGGACGATGAGGTTTCAGGGAGAGTGACCAGGGTTGTCAATGGTGGCGGTGAGGTCCGGTCAGAACCGGTGACCGCAGAGAACGTCGGCTCGACGGCGGTCGTTGCGCTTGTCTGCTCATCTCATGTGGTGGTTGCCAACTGTGGAGATTCGCGCATCGTGCTCTGCCGCGGGAAGGAGCCCGTAGCCTTGTCAATTGATCACAAGCCTGACAGGAAGGATGAGCGGGCAAGGATTGAAGCCCAGGGAGGCAAGGTCATCCAATGGAATGGTTACCGGGTGTCCGGTATACTTGCTATGTCCCGATCAATCGGTGATCGCTATCTGAAACCATTTGTCATTCCAAAACCGGAAGTTATGGTCGTTCCACGGGCGAAGGATGATGACTGTCTTATTCTAGCAAGCGATGGGCTGTGGGATGTTGTGTCAAATGAAGAGGCATGCAAAGTCGCACGCCGACAGATCCTTCTGTGGCACAAGAACAATGGCGCTGCATCACCATTGTCTGATGAGGGTGAAGGATCCACCGACCCTGCTGCCCAAGCAGCTGCCGATTATCTGATGAGACTCGCTCTGAAGAAAGGCAGCGAGGATAACATCACTGTCATTGTTGTCGACTTGAAACCGCGAAAGAAACTCAAGAACATTTCATAACAGGGCCTGGGTTCATCTTGAGTAGCTGACCATGATGTGTGTCAAAGAGTTTCATACAACTAGTCGGTCTTCCTCACGGGACTTGACCCACTGTTTTTTTTGCTGGGCTTCAAGAAATTGTACATGTATATGCTAGATGTATCTTCCATCTAGGGATAAATATCTACCTACCTCCATTCTATCCCGTCAGCTTCAGTTTTCAGCCCTGGCAAGAAGTATCCCTTGTACAATAATTGAACTTGGAAAAAGCTAAGTGGCTGAGATGACCAGTAAAATTAACATAATTGTGATCACCTTTCCTGAA
CDS Sequence
  • >LOC_Os05g46040.1
    ATGGCGGCGGCGGCGGCGGCGGCGGCGATATGTGGGGAGGATGAGACGGCGGCGCGGGTGGGGTGCACGGGGGAATGGGCGGGCGGGATCGAGAGGGTGGATCTTGGGGAGAGGAAGGAGGCGGTGGCGGCGGCGGGGGCGGGGAAGAGGAGCGTCTACCTGATGGACTGCGCGCCGGTGTGGGGCTGCGCGTCGACGCGCGGCCGCAGCGCGGAGATGGAGGACGCGAGCGCCGCCGTGCCGCGGTTCGCGGACGTGCCGGTGCGGCTGCTCGCCAGCCGGCGCGACCTCGACGCGCTGGGCCTCGACGCCGACGCGCTCCGCCTGCCGGCGCACCTCTTCGGCGTGTTCGACGGCCACGGCGGCGCCGAGGTGGCGAACTACTGCCGTGAAAGGATCCACGTCGTCTTGAGCGAGGAGCTGAAGCGACTTGGCAAGAATTTGGGGGAGATGGGCGAGGTGGACATGAAAGAGCACTGGGATGATGTGTTCACGAAATGTTTCCAGAGAGTGGACGATGAGGTTTCAGGGAGAGTGACCAGGGTTGTCAATGGTGGCGGTGAGGTCCGGTCAGAACCGGTGACCGCAGAGAACGTCGGCTCGACGGCGGTCGTTGCGCTTGTCTGCTCATCTCATGTGGTGGTTGCCAACTGTGGAGATTCGCGCATCGTGCTCTGCCGCGGGAAGGAGCCCGTAGCCTTGTCAATTGATCACAAGCCTGACAGGAAGGATGAGCGGGCAAGGATTGAAGCCCAGGGAGGCAAGGTCATCCAATGGAATGGTTACCGGGTGTCCGGTATACTTGCTATGTCCCGATCAATCGGTGATCGCTATCTGAAACCATTTGTCATTCCAAAACCGGAAGTTATGGTCGTTCCACGGGCGAAGGATGATGACTGTCTTATTCTAGCAAGCGATGGGCTGTGGGATGTTGTGTCAAATGAAGAGGCATGCAAAGTCGCACGCCGACAGATCCTTCTGTGGCACAAGAACAATGGCGCTGCATCACCATTGTCTGATGAGGGTGAAGGATCCACCGACCCTGCTGCCCAAGCAGCTGCCGATTATCTGATGAGACTCGCTCTGAAGAAAGGCAGCGAGGATAACATCACTGTCATTGTTGTCGACTTGAAACCGCGAAAGAAACTCAAGAACATTTCATAA
  • >LOC_Os05g46040.2
    ATGGCGGCGGCGGCGGCGGCGGCGGCGATATGTGGGGAGGATGAGACGGCGGCGCGGGTGGGGTGCACGGGGGAATGGGCGGGCGGGATCGAGAGGGTGGATCTTGGGGAGAGGAAGGAGGCGGTGGCGGCGGCGGGGGCGGGGAAGAGGAGCGTCTACCTGATGGACTGCGCGCCGGTGTGGGGCTGCGCGTCGACGCGCGGCCGCAGCGCGGAGATGGAGGACGCGAGCGCCGCCGTGCCGCGGTTCGCGGACGTGCCGGTGCGGCTGCTCGCCAGCCGGCGCGACCTCGACGCGCTGGGCCTCGACGCCGACGCGCTCCGCCTGCCGGCGCACCTCTTCGGCGTGTTCGACGGCCACGGCGGCGCCGAGGTGGCGAACTACTGCCGTGAAAGGATCCACGTCGTCTTGAGCGAGGAGCTGAAGCGACTTGGCAAGAATTTGGGGGAGATGGGCGAGGTGGACATGAAAGAGCACTGGGATGATGTGTTCACGAAATGTTTCCAGAGAGTGGACGATGAGGTTTCAGGGAGAGTGACCAGGGTTGTCAATGGTGGCGGTGAGGTCCGGTCAGAACCGGTGACCGCAGAGAACGTCGGCTCGACGGCGGTCGTTGCGCTTGTCTGCTCATCTCATGTGGTGGTTGCCAACTGTGGAGATTCGCGCATCGTGCTCTGCCGCGGGAAGGAGCCCGTAGCCTTGTCAATTGATCACAAGCCTGACAGGAAGGATGAGCGGGCAAGGATTGAAGCCCAGGGAGGCAAGGTCATCCAATGGAATGGTTACCGGGTGTCCGGTATACTTGCTATGTCCCGATCAATCGGTGATCGCTATCTGAAACCATTTGTCATTCCAAAACCGGAAGTTATGGTCGTTCCACGGGCGAAGGATGATGACTGTCTTATTCTAGCAAGCGATGGGCTGTGGGATGTTGTGTCAAATGAAGAGGCATGCAAAGTCGCACGCCGACAGATCCTTCTGTGGCACAAGAACAATGGCGCTGCATCACCATTGTCTGATGAGGGTGAAGGATCCACCGACCCTGCTGCCCAAGCAGCTGCCGATTATCTGATGAGACTCGCTCTGAAGAAAGGCAGCGAGGATAACATCACTGTCATTGTTGTCGACTTGAAACCGCGAAAGAAACTCAAGAACATTTCATAA
Protein Sequence
  • >LOC_Os05g46040.1
    MAAAAAAAAICGEDETAARVGCTGEWAGGIERVDLGERKEAVAAAGAGKRSVYLMDCAPVWGCASTRGRSAEMEDASAAVPRFADVPVRLLASRRDLDALGLDADALRLPAHLFGVFDGHGGAEVANYCRERIHVVLSEELKRLGKNLGEMGEVDMKEHWDDVFTKCFQRVDDEVSGRVTRVVNGGGEVRSEPVTAENVGSTAVVALVCSSHVVVANCGDSRIVLCRGKEPVALSIDHKPDRKDERARIEAQGGKVIQWNGYRVSGILAMSRSIGDRYLKPFVIPKPEVMVVPRAKDDDCLILASDGLWDVVSNEEACKVARRQILLWHKNNGAASPLSDEGEGSTDPAAQAAADYLMRLALKKGSEDNITVIVVDLKPRKKLKNIS*
  • >LOC_Os05g46040.2
    MAAAAAAAAICGEDETAARVGCTGEWAGGIERVDLGERKEAVAAAGAGKRSVYLMDCAPVWGCASTRGRSAEMEDASAAVPRFADVPVRLLASRRDLDALGLDADALRLPAHLFGVFDGHGGAEVANYCRERIHVVLSEELKRLGKNLGEMGEVDMKEHWDDVFTKCFQRVDDEVSGRVTRVVNGGGEVRSEPVTAENVGSTAVVALVCSSHVVVANCGDSRIVLCRGKEPVALSIDHKPDRKDERARIEAQGGKVIQWNGYRVSGILAMSRSIGDRYLKPFVIPKPEVMVVPRAKDDDCLILASDGLWDVVSNEEACKVARRQILLWHKNNGAASPLSDEGEGSTDPAAQAAADYLMRLALKKGSEDNITVIVVDLKPRKKLKNIS*