Gene Details:

Functional Descriptions:

  • Notably, the expression of OsHXK5, OsHXK6, or their mutant alleles complemented the Arabidopsis glucose insensitive2-1 mutant, thereby resulting in wild-type characteristics in seedling development, Glc-dependent gene expression, and plant growth.
  • Furthermore, transgenic rice plants overexpressing OsHXK5 or OsHXK6 exhibited hypersensitive plant growth retardation and enhanced repression of the photosynthetic gene RbcS in response to Glc treatment.
  • Transient expression analyses using GFP fusion constructs revealed that OsHXK5 and OsHXK6 are associated with mitochondria.
  • Interestingly, the OsHXK5DeltamTP-GFP and OsHXK6DeltamTP-GFP fusion proteins, which lack N-terminal mitochondrial targeting peptides, were present mainly in the nucleus with a small amount of the proteins seen in the cytosol.
  • In addition, the OsHXK5NLS-GFP and OsHXK6NLS-GFP fusion proteins harboring nuclear localization signals were targeted predominantly in the nucleus, suggesting that these OsHXKs retain a dual-targeting ability to mitochondria and nuclei.
  • Sugars induced the expression of three OsHXKs, OsHXK2, OsHXK5, and OsHXK6, in excised leaves, while suppressing OsHXK7 expression in excised leaves and immature seeds.
  • Our results demonstrate that OsHXK5 contributes to a large portion of the hexokinase activity necessary for the starch utilization pathway during pollen germination and tube growth, as well as for starch biosynthesis during pollen maturation.
  • We characterized hxk5-1, a Tos17 mutant of OsHXK5 that is up-regulated in maturing pollen, a stage when starch accumulates.

Literature:

Gene Resources:

Sequences:

cDNA Sequence
  • >LOC_Os05g44760.1
    ACCGGTTAAAACGCCCAAGTCAGTGGCTCTCGTCCTCCTTTCTCCTACGCGGCCGGAGAGAGGAGGGGGGAAGAGAGGATCTCGCAGAGCGCCATAGCCTCGGATCCAGAAGCGGAATTCGGTGGAGGTCTCGGGCACCTGGATCGATCGGAGGAAGGGAAGGCGGAGCAGCGGTGATGGGGAAGGCGGCGGCGGTGGGGACGGCGGTGGTGGTGGCCGCGGCGGTCGGGGTGGCGGTGGTGCTGGCGCGGAGGCGGAGGAGGAGGGACCTGGAGCTGGTGGAGGGAGCCGCGGCGGAGAGGAAGAGGAAGGTGGCGGCGGTGATCGAGGACGTGGAGCACGCGCTGTCGACCCCGACGGCGCTGCTGCGGGGCATCTCGGACGCCATGGTCACCGAGATGGAGCGAGGCCTGCGCGGGGACAGCCACGCCATGGTTAAGATGCTCATCACCTACGTCGACAACCTCCCCACCGGAAATGAACAGGGGTTGTTTTATGCATTGGATCTTGGAGGAACCAACTTCCGCGTCCTGCGAGTCCAACTCGGTGGCAAGGAGAAACGTGTCGTCCAACAACAGTACGAGGAAGTCTCAATTCCACCACATCTGATGGTTGGAACTTCCATGGAACTGTTTGATTTTATTGCTTCTGCATTGTCAAAATTTGTTGATACTGAAGGTGATGATTTCCACCTCCCAGAAGGGAGACAGAGAGAGCTGGGCTTCACCTTTTCCTTCCCAGTGAGCCAGACATCAATATCGTCAGGAACGCTCATCAAGTGGACAAAGGGTTTCTCCATCAATGACGCGGTTGGCGAAGATGTTGTATCTGAGTTGGGCAAGGCCATGGAGAGGCAGGGATTAGATATGAAAATTGCAGCATTGGTTAATGACACTGTCGGCACATTGGCTGGTGGGAGGTATGCGGATAACAGTGTTGTTGCTGCTATAATATTGGGCACTGGTACAAATGCAGCATATGTTGAGAATGCTAATGCAATTCCTAAATGGACCGGTTTACTGCCTAGGTCCGGAAATATGGTAATCAACACAGAATGGGGGAGCTTTAAATCAGATAAGCTTCCTCTTTCAGAATTCGATAAAGCAATGGATTTTGAAAGCTTGAATCCTGGAGAGCAGATATATGAAAAGTTGATTTCTGGCATGTATCTTGGAGAGATAGTGCGAAGAATCTTGCTGAAGCTTGCTCATGATGCAGCTTTGTTTGGGGATGTTGTTCCATCTAAGCTAGAGCAACCGTTTGTACTAAGGACACCGGATATGTCAGCCATGCATCATGACTCGTCACATGACCTTAAAACTGTTGGAGCTAAGCTAAAGGATATCGTCGGGGTCCCAGATACTTCCCTGGAAGTAAGATACATTACCAGTCACATTTGTGACATAGTTGCAGAGCGTGCTGCACGCTTGGCTGCTGCTGGCATATATGGGGTCCTAAAGAAGCTAGGTCGGGACAAGATGCCAAAAGACGGCAGTAAGATGCCTAGGACTGTCATTGCCTTGGATGGTGGGCTCTATGAACATTACAAGAAGTTCAGCAGTTGCTTAGAATCAACTCTAACAGACCTTCTTGGGGATGATGTCTCGTCTTCGGTGGTTACCAAGCTGGCCAACGATGGTTCTGGCATTGGAGCTGCTCTTCTCGCAGCCTCGCACTCCCAGTATGCCGAGATCGACTAGCTTTAAGGATGATCTTGATGAATGATGAATCAAACTCCGTTTGTAGGTTCTCATTTCCCCCTTCAAAATCCACATAATACTCCTGGCTCCCCCCTTGAAATCTTACCATCTTTTTTTGGCTATTCTGAGGGCAAACATAAGTGCCTCTGCAGCGGGATATAGCTAGTATAGCGCCAATGAGTTTGGAGGTTTTCTAATGGCATAAAACGTTGGATGGCAGTAGCAGACTAACAGGGAAATGGAGGCACAGGCAATTTCCATTCCTGTTCTGTCAGATTCTTTTCCCCCTTAATTGATGTTGAGAACCAAGATTTTTTTGCTCTGTATTTTCTCTTCGTAATAAAGAAGGGGACATAATCTAATTGCTCTTGTTTGATCTCATATATGTATGTGACTGCACTCTCACTGTCCCAAAATTTTATAGATGCATTTACAAAGTTACAATACTCATTACATGCAAATAACTGAAGGATGGCGAATGTATTTGTCTGGTATCTACCTGCTCTCCTAACTAGCGTGCCATCTCTCCTAGATGGTAACCATTGGATCAGGTTATACTTCTTATTGACCATTGTTCTCATACTATGCTGTCCAAGCTGCCGTTGCCGTCGTCGATGCCTTTGGTGTCCGAAAGCTGCTTCCGGCGGCTGCCACCCCTGATCGGCAGAAACGGCGCGCCGTCGCCGTCGTACTCCGCCACCTTCCCGTCCACGGTGTTCTGGAGCGCCTGCTCGCACCTCACCAGAATCTGCAGAACGTCCCTCATTGTCGGCCTCGTCGCCGGCTGCGCGCCGGTGCAGATGATGCCGAGCTTGAACACGACCTCGGCGTCGTCGCCGTACCCGGAGTCGGTGATGCACCTGTCCACCGCGTCGGCGATGCTCCTGCCCGATTGCAGGTGCCGCCACGCCCACTCCGCCAGCGAGCCGTGCTCGCCGCCGTCGTGGGCTTCCCTGCCGGTGATCAGCTCGAGGAGCACCACCCCGAAGCTGTACACGTCGACCTTCTCGTTCACCTTCCTCGTGTACGCGCACTCTGCA
CDS Sequence
  • >LOC_Os05g44760.1
    ATGGGGAAGGCGGCGGCGGTGGGGACGGCGGTGGTGGTGGCCGCGGCGGTCGGGGTGGCGGTGGTGCTGGCGCGGAGGCGGAGGAGGAGGGACCTGGAGCTGGTGGAGGGAGCCGCGGCGGAGAGGAAGAGGAAGGTGGCGGCGGTGATCGAGGACGTGGAGCACGCGCTGTCGACCCCGACGGCGCTGCTGCGGGGCATCTCGGACGCCATGGTCACCGAGATGGAGCGAGGCCTGCGCGGGGACAGCCACGCCATGGTTAAGATGCTCATCACCTACGTCGACAACCTCCCCACCGGAAATGAACAGGGGTTGTTTTATGCATTGGATCTTGGAGGAACCAACTTCCGCGTCCTGCGAGTCCAACTCGGTGGCAAGGAGAAACGTGTCGTCCAACAACAGTACGAGGAAGTCTCAATTCCACCACATCTGATGGTTGGAACTTCCATGGAACTGTTTGATTTTATTGCTTCTGCATTGTCAAAATTTGTTGATACTGAAGGTGATGATTTCCACCTCCCAGAAGGGAGACAGAGAGAGCTGGGCTTCACCTTTTCCTTCCCAGTGAGCCAGACATCAATATCGTCAGGAACGCTCATCAAGTGGACAAAGGGTTTCTCCATCAATGACGCGGTTGGCGAAGATGTTGTATCTGAGTTGGGCAAGGCCATGGAGAGGCAGGGATTAGATATGAAAATTGCAGCATTGGTTAATGACACTGTCGGCACATTGGCTGGTGGGAGGTATGCGGATAACAGTGTTGTTGCTGCTATAATATTGGGCACTGGTACAAATGCAGCATATGTTGAGAATGCTAATGCAATTCCTAAATGGACCGGTTTACTGCCTAGGTCCGGAAATATGGTAATCAACACAGAATGGGGGAGCTTTAAATCAGATAAGCTTCCTCTTTCAGAATTCGATAAAGCAATGGATTTTGAAAGCTTGAATCCTGGAGAGCAGATATATGAAAAGTTGATTTCTGGCATGTATCTTGGAGAGATAGTGCGAAGAATCTTGCTGAAGCTTGCTCATGATGCAGCTTTGTTTGGGGATGTTGTTCCATCTAAGCTAGAGCAACCGTTTGTACTAAGGACACCGGATATGTCAGCCATGCATCATGACTCGTCACATGACCTTAAAACTGTTGGAGCTAAGCTAAAGGATATCGTCGGGGTCCCAGATACTTCCCTGGAAGTAAGATACATTACCAGTCACATTTGTGACATAGTTGCAGAGCGTGCTGCACGCTTGGCTGCTGCTGGCATATATGGGGTCCTAAAGAAGCTAGGTCGGGACAAGATGCCAAAAGACGGCAGTAAGATGCCTAGGACTGTCATTGCCTTGGATGGTGGGCTCTATGAACATTACAAGAAGTTCAGCAGTTGCTTAGAATCAACTCTAACAGACCTTCTTGGGGATGATGTCTCGTCTTCGGTGGTTACCAAGCTGGCCAACGATGGTTCTGGCATTGGAGCTGCTCTTCTCGCAGCCTCGCACTCCCAGTATGCCGAGATCGACTAG
Protein Sequence
  • >LOC_Os05g44760.1
    MGKAAAVGTAVVVAAAVGVAVVLARRRRRRDLELVEGAAAERKRKVAAVIEDVEHALSTPTALLRGISDAMVTEMERGLRGDSHAMVKMLITYVDNLPTGNEQGLFYALDLGGTNFRVLRVQLGGKEKRVVQQQYEEVSIPPHLMVGTSMELFDFIASALSKFVDTEGDDFHLPEGRQRELGFTFSFPVSQTSISSGTLIKWTKGFSINDAVGEDVVSELGKAMERQGLDMKIAALVNDTVGTLAGGRYADNSVVAAIILGTGTNAAYVENANAIPKWTGLLPRSGNMVINTEWGSFKSDKLPLSEFDKAMDFESLNPGEQIYEKLISGMYLGEIVRRILLKLAHDAALFGDVVPSKLEQPFVLRTPDMSAMHHDSSHDLKTVGAKLKDIVGVPDTSLEVRYITSHICDIVAERAARLAAAGIYGVLKKLGRDKMPKDGSKMPRTVIALDGGLYEHYKKFSSCLESTLTDLLGDDVSSSVVTKLANDGSGIGAALLAASHSQYAEID*