Gene Details:

• MSU gene ID: LOC_Os05g44340
• RAPdb gene ID: Os05g0519700
• Gene Symbol: HSP101 OsClpB-cyt HSP100
• Genome: MSU7 ,  IRGSP-1.0
• Species: Oryza sativa

Functional Descriptions:

• Our results showed that a positive feedback loop formed by two heat-inducible genes, HEAT SHOCK PROTEIN101 (HSP101) and HEAT STRESS-ASSOCIATED 32-KD PROTEIN (HSA32), at the posttranscriptional level prolongs the effect of heat acclimation in rice seedlings.
• Rice HSP101 cDNA expression in hsp104 deficient yeast also caused recovery in tolerance against arsenite.
• The interplay between HSP101 and HSA32 also affects basal thermotolerance of rice seeds.
• In the temperature regime tested, 45 degrees C treatment to intact rice seedlings for 2 h showed maximal levels of HSP101 mRNA.
• Transgenic rice plants produced with 2 kb OsClpB-cyt promoter driving Gus reporter gene showed heat- and metal-regulated Gus expression in vegetative tissues and constitutive Gus expression in calli, flowering tissues, and embryonal half of seeds.
• OsClpB-cyt transcript is shown to be enriched at milk and dough stages of seed development.
• Rice seedlings regenerated with OsClpB-cyt promoter fragment with deletion of its canonical heat shock element sequence (HSE(-273 to -280)) showed not only heat shock inducibility of Gus transcript/protein but also constitutive expression of Gus in vegetative tissues.
• While Os05g44340 encodes cytoplasmic HSP100 protein, those encoded by the other four genes are predicted to have chloroplast transit peptides.

Function-related keywords:

• seedling ,  arsenite ,  seed ,  flower ,  vegetative ,  chloroplast ,  seed-development ,  temperature

Literature:

• Molecular characterization of rice hsp101: complementation of yeast hsp104 mutation by disaggregation of protein granules and differential expression in indica and japonica rice types .  DOI:  10.1023/a:1022324920316 ;  PMID:  12650620
• OsHsfA2c and OsHsfB4b are involved in the transcriptional regulation of cytoplasmic OsClpB (Hsp100) gene in rice (Oryza sativa L.) .  DOI:  10.1007/s12192-011-0303-5 ;  PMID:  22147560
• Genome-wide analysis of rice ClpB/HSP100, ClpC and ClpD genes .  DOI:  10.1186/1471-2164-11-95 ;  PMID:  20141629
• A positive feedback loop between HEAT SHOCK PROTEIN101 and HEAT STRESS-ASSOCIATED 32-KD PROTEIN modulates long-term acquired thermotolerance illustrating diverse heat stress responses in rice varieties .  DOI:  10.1104/pp.113.229609 ;  PMID:  24520156
• Complexity of rice Hsp100 gene family: lessons from rice genome sequence data .  DOI:  10.1007/s12038-007-0060-x ;  PMID:  17536180

Related News:

Gene Resources:

• NCBI ID:
• UniProt accessions:

Sequences: