Gene Details:

Functional Descriptions:

  • The synaptonemal complex is well installed in OsMSH5, while the chiasma frequency is greatly reduced to approximately 10% of that observed in the wild-type, leading to the homologous non-disjunction and complete sterile phenotype.
  • Immunofluorescence studies indicate that OsMSH5 chromosomal localization is limited to the early meiotic prophase I.
  • OsMSH5 can be loaded onto meiotic chromosomes in Oszip4, Osmer3, and hei10.
  • Here, we report the identification and characterization of its rice homolog, OsMSH5, and demonstrate its function in rice meiosis.
  • The role of OsMSH5 in crossover formation during rice meiosis.
  • OsMSH5 is predominantly expressed in panicles.
  • Five independent OsMSH5 mutants exhibited normal vegetative growth and severe sterility.
  • The MSH4-MSH5 heterodimer has been demonstrated in mammals to stabilize the formation of progenitor and double Holliday junctions that may be resolved as crossovers (COs).
  • Crossover Formation During Rice Meiosis Relies on Interaction of OsMSH4 and OsMSH5.

Literature:

Gene Resources:

  • NCBI ID:
  • UniProt accessions:

Sequences:

cDNA Sequence
  • >LOC_Os05g41880.1
    AACAACTTCCTTCCCGTACAAGCCAGTGCCCGAGCCACGCTCCCGCCGCACTTCGCCCCTCGCCGCCTAGCTTGCTCCATCCTCCGCGCCCGCGCAGCTGCGCTCGCCGTCGCCGTCGCTGCCTAGCTTCCTCCAGCCTCCGCATCTGCGCTCTATTTGTACCGCATTCTCGGAGGAGGAGCAGGAGGCGAGGAGCCGCCGAGTAGAGGAGAGGGGGAGGCGAAGTCGGTGCTCTCGCCGGGGGTTGACGGTGGAACTCCGGGCGAGCGATCGAGCCGGCGAAATGGACGAGGAAGAGGAGGAGGAGATGTCCGAGAGGGAGGTCGACTCCCAGGTTCATATGGCTTGTGTAATGCAAGGACGCAGAGTTGGAATAGCATACTATGATTCAAGTATGCACCAGCTTTTTGTATTGGAAATCTGGGAAGACATAACTGAAGATTTTCCTTTGATTGATCTTGTGAAATATCAATCCAAACCGAGCACAATATACACAAGTACTAAAACAGATGAGGCACTTTTGTTGGCGTTACAACGAAATGATTGTAATGATGAGGCCCCTGCTGTGAAGCTCATGAAAAGCTCAACCTTCAGCTATGAGCAAGCCTGGCACAGATTAATGTACTTGAAAGTCGCAGCTATGGACGAAGGGTTAAGTGTAAAAGAAAGGATCTGTTTTCTTAATTCCATGATGGATCTTGGAAGTGACGTTCAAGTTCGTGCTGCTGGAGGTCTTCTTGCTATATTAGACAACGAGAGGCTTCTTGATACCCTTGATCAGATGGAAGGTGGAGCATCCATTGCTATTGATTCTGTGGCACAGATATCATTTTATCCTTTTATCCGTAATGATATTATACGGACTAAATGTGCTAATGTACTGATCAGCATGTTTTTGTGGTTTTACTTTGGAAACAAGTTAGAAAGCTATTTATGTAGGATTGTAGTGCGTCACTCCTATGGGAAAACATCTCTTGAGGTCACTGTTACTTTCTATAATCATGATTGCAGATTTGCAAAATTCAACTCTCCAAGTTCATTCTGTACAAGCAGTGACTGGCATGCATTTCTGAAGTGCATTTGCTCTCTTCTGCATATAAACAAGATTTTTGAAGTAGGAATATCAGAGCATTTGGCAATCAAATTACAACATATGAACATTGACTTGGTTGGAAAGGCCAATTCTTCCATTACAGAAGAGCTGGATTATGTATCAGATCTGGTAGTTGGTGTGATTGATGTGCAACGGGGTAAAGAAAAAGGCTACGACACACTGGTGAAAGACGGATTATGTGAGGAGTTAGACGAACTGAGGATGGTCTATGAAGAATTGCCTGATTTTTTGGAGCAGGTGTCAGCCAATGAAATTGCCTCCTTCCCTTTCTCATTTGAATGCAGAAAAGCTCCTCTAATCGTCTATGTACACCAAATAGGATATTTAATGTGTTTTTTCGATGAGAAGATAAGTGATGCCTTGTTGATAGGACTTCCAGATTTTGAATTTGCGGAAATGACTGTTGATACCTTCGTTCCAAATGACACTAAAATCCGCAGTTCAGGACGAATCAACATAATAACTGGACCTAACTACTCAGGGAAAAGCATTTACATCAAGCAGGTGGCTTTGGTGGTTTTTCTTGCTCATATTGGAAGCTTTGTTCCTGCTGATTCTGCCATTGTTGGATTGACAGATAGGATTTTCTGTGCAATGGGCAGCAAATCCATGACAAGTGAACAGTCAACTTTTATGATTGATTTACATCAAGTTGGAACAATGCTCAGATGGTATTGGTCTGCTTGGAGGAACTATCAGCCATTTTACAGACTACGATTGTCCTCCAAAGGTGCTAGCTTGGAAAAATGTTGCTACTGA
CDS Sequence
  • >LOC_Os05g41880.1
    ATGGACGAGGAAGAGGAGGAGGAGATGTCCGAGAGGGAGGTCGACTCCCAGGTTCATATGGCTTGTGTAATGCAAGGACGCAGAGTTGGAATAGCATACTATGATTCAAGTATGCACCAGCTTTTTGTATTGGAAATCTGGGAAGACATAACTGAAGATTTTCCTTTGATTGATCTTGTGAAATATCAATCCAAACCGAGCACAATATACACAAGTACTAAAACAGATGAGGCACTTTTGTTGGCGTTACAACGAAATGATTGTAATGATGAGGCCCCTGCTGTGAAGCTCATGAAAAGCTCAACCTTCAGCTATGAGCAAGCCTGGCACAGATTAATGTACTTGAAAGTCGCAGCTATGGACGAAGGGTTAAGTGTAAAAGAAAGGATCTGTTTTCTTAATTCCATGATGGATCTTGGAAGTGACGTTCAAGTTCGTGCTGCTGGAGGTCTTCTTGCTATATTAGACAACGAGAGGCTTCTTGATACCCTTGATCAGATGGAAGGTGGAGCATCCATTGCTATTGATTCTGTGGCACAGATATCATTTTATCCTTTTATCCGTAATGATATTATACGGACTAAATGTGCTAATGTACTGATCAGCATGTTTTTGTGGTTTTACTTTGGAAACAAGTTAGAAAGCTATTTATGTAGGATTGTAGTGCGTCACTCCTATGGGAAAACATCTCTTGAGGTCACTGTTACTTTCTATAATCATGATTGCAGATTTGCAAAATTCAACTCTCCAAGTTCATTCTGTACAAGCAGTGACTGGCATGCATTTCTGAAGTGCATTTGCTCTCTTCTGCATATAAACAAGATTTTTGAAGTAGGAATATCAGAGCATTTGGCAATCAAATTACAACATATGAACATTGACTTGGTTGGAAAGGCCAATTCTTCCATTACAGAAGAGCTGGATTATGTATCAGATCTGGTAGTTGGTGTGATTGATGTGCAACGGGGTAAAGAAAAAGGCTACGACACACTGGTGAAAGACGGATTATGTGAGGAGTTAGACGAACTGAGGATGGTCTATGAAGAATTGCCTGATTTTTTGGAGCAGGTGTCAGCCAATGAAATTGCCTCCTTCCCTTTCTCATTTGAATGCAGAAAAGCTCCTCTAATCGTCTATGTACACCAAATAGGATATTTAATGTGTTTTTTCGATGAGAAGATAAGTGATGCCTTGTTGATAGGACTTCCAGATTTTGAATTTGCGGAAATGACTGTTGATACCTTCGTTCCAAATGACACTAAAATCCGCAGTTCAGGACGAATCAACATAATAACTGGACCTAACTACTCAGGGAAAAGCATTTACATCAAGCAGGTGGCTTTGGTGGTTTTTCTTGCTCATATTGGAAGCTTTGTTCCTGCTGATTCTGCCATTGTTGGATTGACAGATAGGATTTTCTGTGCAATGGGCAGCAAATCCATGACAAGTGAACAGTCAACTTTTATGATTGATTTACATCAAGTTGGAACAATGCTCAGATGGTATTGGTCTGCTTGGAGGAACTATCAGCCATTTTACAGACTACGATTGTCCTCCAAAGGTGCTAGCTTGGAAAAATGTTGCTACTGA
Protein Sequence
  • >LOC_Os05g41880.1
    MDEEEEEEMSEREVDSQVHMACVMQGRRVGIAYYDSSMHQLFVLEIWEDITEDFPLIDLVKYQSKPSTIYTSTKTDEALLLALQRNDCNDEAPAVKLMKSSTFSYEQAWHRLMYLKVAAMDEGLSVKERICFLNSMMDLGSDVQVRAAGGLLAILDNERLLDTLDQMEGGASIAIDSVAQISFYPFIRNDIIRTKCANVLISMFLWFYFGNKLESYLCRIVVRHSYGKTSLEVTVTFYNHDCRFAKFNSPSSFCTSSDWHAFLKCICSLLHINKIFEVGISEHLAIKLQHMNIDLVGKANSSITEELDYVSDLVVGVIDVQRGKEKGYDTLVKDGLCEELDELRMVYEELPDFLEQVSANEIASFPFSFECRKAPLIVYVHQIGYLMCFFDEKISDALLIGLPDFEFAEMTVDTFVPNDTKIRSSGRINIITGPNYSGKSIYIKQVALVVFLAHIGSFVPADSAIVGLTDRIFCAMGSKSMTSEQSTFMIDLHQVGTMLRWYWSAWRNYQPFYRLRLSSKGASLEKCCY*