Gene Details:

Functional Descriptions:

  • Although Oryza sativa CCaMK (OsCCaMK) is required for fungal accommodation in rice roots, little is known about the role of OsCCaMK in rice symbiosis with bacteria.
  • Thus, CH4 oxidation and N2 fixation were simultaneously activated in the root zone of WT rice in the LN field and both processes are likely controlled by OsCCaMK.
  • In the present study, the role of rice CCaMK, OsDMI3, in ABA-induced antioxidant defense was investigated in leaves of rice (Oryza sativa) plants.
  • Our data reveal that OsDMI3 is an important component in ABA-induced antioxidant defense in rice.
  • OsDMI3 is a novel component of abscisic acid signaling in the induction of antioxidant defense in leaves of rice.
  • ABA treatment induced the expression of OsDMI3 and OsMPK1 and the activities of OsDMI3 and OsMPK1 in rice leaves.
  • Our data indicate that there exists a cross-talk between OsDMI3 and OsMPK1 in ABA signalling, in which OsDMI3 functions upstream of OsMPK1 to regulate the activities of antioxidant enzymes and the production of H2 O2 in rice.
  • Further, the oxidative damage induced by higher concentrations of PEG and H(2)O(2) was aggravated in the mutant of OsDMI3.
  • Treatments with ABA, H(2)O(2), and polyethylene glycol (PEG) induced the expression of OsDMI3 and the activity of OsDMI3, and H(2)O(2) is required for the ABA-induced increases in the expression and the activity of OsDMI3 under water stress.
  • Here, we report the effect of a Tos17-induced OsCCaMK mutant (NE1115) on CH4 flux in low-nitrogen (LN) and standard-nitrogen (SN) paddy fields compared with wild-type (WT) Nipponbare.
  • Moreover, the analysis of the RNAi silencing of OsDMI3 in protoplasts and the mutant of OsDMI3 showed that higher levels of H(2)O(2) accumulation require OsDMI3 activation in ABA signaling, but the initial H(2)O(2) production induced by ABA is not dependent on the activation of OsDMI3 in leaves of rice plants.
  • This was consistent with the results of Na+ content and NMT that indicated OsDMI3 promoted root elongation under saline-alkaline stress by reducing root Na+ and H+ influx.
  • Calcium/calmodulin-dependent protein kinase OsDMI3 positively regulates saline-alkaline tolerance in rice roots.
  • Collectively, our results suggest that OsDMI3 could promote saline-alkaline tolerance in rice roots by modulating the Na+ and H+ influx.
  • Moreover, real-time RT-PCR analysis revealed that OsDMI3 up-regulated the transcript levels of OsSOS1 and PM-H+-ATPase genes OsA3 and OsA8 in saline-alkaline stressed rice plants.
  • We measured the OsDMI3 activity by an in-gel kinase assay, Na+ content by NaHCO3 treatment, and Na+ and H+ fluxes by noninvasive micro-test technology (NMT).
  • OsDMI3 was found to directly phosphorylate Thr-25 in the N-terminus of OsMKK1, and this Thr-25 phosphorylation is OsDMI3-specific in ABA signaling.
  • These findings reveal OsUXS3 as a direct target of OsDMI3 and demonstrate its involvement in antioxidant defense.
  • Genetic and biochemical evidence demonstrated that OsUXS3 acts downstream of OsDMI3 to enhance the oxidative stress tolerance conferred by higher catalase (CAT) activity.
  • Our results indicate that OsDMI3 promotes the association of OsUXS3 with OsCATB to enhance CAT activity under oxidative stress.

Literature:

Gene Resources:

Sequences:

cDNA Sequence
  • >LOC_Os05g41090.1
    ACTGATCAGGTCTTCAGGAGAAGGAGCTTGCTTTGTACTCCTAACTGCTCCTTCCAGACACAGCTTTGCTACTGGATTACTGCCAATTGAGTCTTGTTATTCGGTTCCTCAATTTATCAAGTTCTTCAGAGCAGGCAGTCGAAGTCCATAATGTCCAAGACTGAGAGCAGAAAGTTGTCCGATGACTATGAAGTTGTGGATGTTCTTGGCCGGGGCGGGTTCTCAATAGTAAGGAGGGGAGTGAGCAAGTCTGAAGAGAAGACCCAGGTTGCCATCAAGACTCTGAGGAGGCTCGGCCCGGCGATGGCGGGTATGAAGCAGGGGACAAAGCCCGTGCCCGGCTCCGGCCTGCCGATGTGGAAACAGGTATCGATCTCCGACGCGCTGCTGACGAACGAGATACTCGTCATGAGGAGGATAGTGGAGAGCGTTGCGCCACATCCTAATGTCATCAACCTGCATGATGTGTATGAAGATGTGCATGGTGTGCACCTCGTCCTTGAGCTGTGCTCGGGTGGTGAGCTTTTTGATCGGATTGTGGGGCGTGACCGGTACTCGGAGTTCGATGCGGCCTGCGTCATTCGTCAGATTGCTAGCGGGCTGGAGGCTCTTCATAAGGCAAGCATTGTACACAGGGACCTGAAGCCGGAGAATTGTCTGTTCTCTGACAAAGATGAGAAGTCCACACTGAAGATCATGGATTTTGGTTTGAGTTCTGTCGAAGATTTCAGTGACCCAATCGTCGCGCTGTTTGGGTCAATAGATTATGTTTCACCGGAGGCTCTATCCAGGCAGGAGGTTTCAGCTGCTAGTGATATGTGGTCTGTTGGGGTAATTCTGTACATTCTTTTATCTGGATGCCCACCATTTCATGCTGCAACCAATCGAGAAAAGCAGCAAAGGATCCTGCAAGGTGAATTCAGTTTTCAGGACCACACATGGAAAACAATATCTTCATCCGCGAAAGATTTGATTTCGCGCCTTCTTTCTGTTCAACCTTACAAAAGGCCAACAGCAAGTGATCTTCTGAGGCATCCTTGGGTGATTGGAGACTGCGCCAAGCAAGATCTCATGGATGCAGAGGTCGTCTCAAAACTGCAAAAGTTCAATGCTAGAAGGAAATTGCGGGCAGCTGCGATAGCCAGCGTTTTGAGCTGCAAAGTGGCATTGAGGACTAAAAGGCTGAGAAATCTTTTAGGAACCCATGACCTTACCTCAGAGGAGCTGGATAATCTGCGGCTTCATTTTGGACGAATATGTGCAGATGGAGAAAACGCCACTCTGTCAGAGTTTGAGCAGGTGCTGAGAGCAATGAAAATGGACTCACTGATTCCCCTAGCTCCCCGCGTATTCGATCTGTTCGACAACAACCGTGATGGAACCGTCGACATGAGAGAGATCCTGTGTGGGTTCTCCAGCCTCAGGAACTCACGAGGCGATGATGCTCTTCGCTTGTGCTTCCAGATGTACGACGCTGATCGGTCAGGCTGCATCAGCAAGGAAGAGCTGGCATCAATGCTCCGAGCGTTGCCGGAGGAGTGCCTGCCAGGCGACATAACGGAGCCGGGGAAGCTGGACGAGGTGTTCGACCAGATGGACGCCGACAGCGACGGCAAGGTCACCTTCGACGAGTTCAAGGCCGCCATGAACAAGGACAGCGCCCTCCAGGACGTCCTCCTCTCCTCCCTCCGCCCACAGTAGCAGCAGCAGCAGCAGCAGAAGCTTCTCAGATTGATCGATTTCTTCAGGAAAAATACTAGTATCTGTACTGCTAATACTTCTGCAGCGAATGGCTGGCTGCCTTATCTGAAATTTTCATCTGTGTCGGCTGCTGAAATGGTAGCAAAACTGTAGCTGTTCTGTTGTCACCTCGTCCGAAAAAATTATGAGCGGTGCGGATTTCACATTTTCACTATATTCTGGGTGAAATTGAATAGCAAGCCGGGTAACATATTTACCTGATTTCCAAAAAAAGAGAAAAAA
CDS Sequence
  • >LOC_Os05g41090.1
    ATGTCCAAGACTGAGAGCAGAAAGTTGTCCGATGACTATGAAGTTGTGGATGTTCTTGGCCGGGGCGGGTTCTCAATAGTAAGGAGGGGAGTGAGCAAGTCTGAAGAGAAGACCCAGGTTGCCATCAAGACTCTGAGGAGGCTCGGCCCGGCGATGGCGGGTATGAAGCAGGGGACAAAGCCCGTGCCCGGCTCCGGCCTGCCGATGTGGAAACAGGTATCGATCTCCGACGCGCTGCTGACGAACGAGATACTCGTCATGAGGAGGATAGTGGAGAGCGTTGCGCCACATCCTAATGTCATCAACCTGCATGATGTGTATGAAGATGTGCATGGTGTGCACCTCGTCCTTGAGCTGTGCTCGGGTGGTGAGCTTTTTGATCGGATTGTGGGGCGTGACCGGTACTCGGAGTTCGATGCGGCCTGCGTCATTCGTCAGATTGCTAGCGGGCTGGAGGCTCTTCATAAGGCAAGCATTGTACACAGGGACCTGAAGCCGGAGAATTGTCTGTTCTCTGACAAAGATGAGAAGTCCACACTGAAGATCATGGATTTTGGTTTGAGTTCTGTCGAAGATTTCAGTGACCCAATCGTCGCGCTGTTTGGGTCAATAGATTATGTTTCACCGGAGGCTCTATCCAGGCAGGAGGTTTCAGCTGCTAGTGATATGTGGTCTGTTGGGGTAATTCTGTACATTCTTTTATCTGGATGCCCACCATTTCATGCTGCAACCAATCGAGAAAAGCAGCAAAGGATCCTGCAAGGTGAATTCAGTTTTCAGGACCACACATGGAAAACAATATCTTCATCCGCGAAAGATTTGATTTCGCGCCTTCTTTCTGTTCAACCTTACAAAAGGCCAACAGCAAGTGATCTTCTGAGGCATCCTTGGGTGATTGGAGACTGCGCCAAGCAAGATCTCATGGATGCAGAGGTCGTCTCAAAACTGCAAAAGTTCAATGCTAGAAGGAAATTGCGGGCAGCTGCGATAGCCAGCGTTTTGAGCTGCAAAGTGGCATTGAGGACTAAAAGGCTGAGAAATCTTTTAGGAACCCATGACCTTACCTCAGAGGAGCTGGATAATCTGCGGCTTCATTTTGGACGAATATGTGCAGATGGAGAAAACGCCACTCTGTCAGAGTTTGAGCAGGTGCTGAGAGCAATGAAAATGGACTCACTGATTCCCCTAGCTCCCCGCGTATTCGATCTGTTCGACAACAACCGTGATGGAACCGTCGACATGAGAGAGATCCTGTGTGGGTTCTCCAGCCTCAGGAACTCACGAGGCGATGATGCTCTTCGCTTGTGCTTCCAGATGTACGACGCTGATCGGTCAGGCTGCATCAGCAAGGAAGAGCTGGCATCAATGCTCCGAGCGTTGCCGGAGGAGTGCCTGCCAGGCGACATAACGGAGCCGGGGAAGCTGGACGAGGTGTTCGACCAGATGGACGCCGACAGCGACGGCAAGGTCACCTTCGACGAGTTCAAGGCCGCCATGAACAAGGACAGCGCCCTCCAGGACGTCCTCCTCTCCTCCCTCCGCCCACAGTAG
Protein Sequence
  • >LOC_Os05g41090.1
    MSKTESRKLSDDYEVVDVLGRGGFSIVRRGVSKSEEKTQVAIKTLRRLGPAMAGMKQGTKPVPGSGLPMWKQVSISDALLTNEILVMRRIVESVAPHPNVINLHDVYEDVHGVHLVLELCSGGELFDRIVGRDRYSEFDAACVIRQIASGLEALHKASIVHRDLKPENCLFSDKDEKSTLKIMDFGLSSVEDFSDPIVALFGSIDYVSPEALSRQEVSAASDMWSVGVILYILLSGCPPFHAATNREKQQRILQGEFSFQDHTWKTISSSAKDLISRLLSVQPYKRPTASDLLRHPWVIGDCAKQDLMDAEVVSKLQKFNARRKLRAAAIASVLSCKVALRTKRLRNLLGTHDLTSEELDNLRLHFGRICADGENATLSEFEQVLRAMKMDSLIPLAPRVFDLFDNNRDGTVDMREILCGFSSLRNSRGDDALRLCFQMYDADRSGCISKEELASMLRALPEECLPGDITEPGKLDEVFDQMDADSDGKVTFDEFKAAMNKDSALQDVLLSSLRPQ*