Information report for EUI1;OsCYP714D1
Gene Details
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Functional Descriptions
- It was found that the level of active gibberellin was elevated in the mutant, whereas its growth in response to gibberellin is similar to that of the wild type, suggesting that the higher level accumulation of gibberellin in the EUI1 mutant causes the abnormal elongation of the uppermost internode.
- Overexpression of EUI1 gave rise to the gibberellin-deficient-like phenotypes, which could be partially reversed by supplementation with gibberellin.
- Furthermore, apart from the alteration of expression levels of the gibberellin biosynthesis genes, accumulation of SLR1 protein was found in the overexpressing transgenic plants, indicating that the expression level of EUI1 is implicated in both gibberellin-mediated SLR1 destruction and a feedback regulation in gibberellin biosynthesis.
- Therefore, we proposed that EUI1 plays a negative role in gibberellin-mediated regulation of cell elongation in the uppermost internode of rice.
- EUI1, encoding a putative cytochrome P450 monooxygenase, regulates internode elongation by modulating gibberellin responses in rice.
- Here, we report a detailed characterization of an elongated uppermost internode1 (EUI1) mutant, which has been used in hybrid rice breeding.
- We cloned the EUI1 gene, which encodes a putative cytochrome P450 monooxygenase, by map-based cloning and found that EUI1 was weakly expressed in most tissues, but preferentially in young panicles.
- The expression of EUI1 is finely tuned, thereby maintaining homeostasis of endogenous bioactive GA and producing plants of normal plant height.
- This complex generates closed chromatin at EUI1, suppressing EUI1 expression and modulating GA homeostasis.
- Here, we identified a dominant dwarf mutant, dEUI1, caused by the deletion of an RY motif-containing cis silencing element (SE1) in the intron of EUI1.
- OsCYP714D1 improves plant growth and salt tolerance through regulating gibberellin and ion homeostasis in transgenic poplar.
- Previously, we reported that expression of the rice cytochrome P450 monooxygenase gene OsCYP714D1 increased gibberellic acid (GA) accumulation and shoot growth in transgenic poplar.
- Our findings imply that OsCYP714D1 participated in the regulation of both shoot growth and salt resistance through regulating gibberellin and ion homeostasis in transgenic poplar, and it can be used as a candidate gene for the engineering of new tree varieties with improved biomass production and salt stress resistance.
- In this work, we demonstrate that expression of OsCYP714D1 improved the salt tolerance of transgenic poplar plants.
- Transcriptomic analyses revealed that OsCYP714D1 expression up-regulated the expressions of GA biosynthesis, signaling and stress responsive genes in transgenic plants under both normal and high salt stress conditions.
- Functional complementation and overexpression tests showed that LG5 can rescue the bigger grain size and larger flag leaf angle in the Xiushui11 (XS) background.
- Knockdown of the LG5 transcription level by RNA interference resulted in elevated grain size and flag leaf angle in the Nipponbare (NIP) background.
- Morphological and cellular analyses suggested that LG5 regulated grain size and flag leaf angle by promoting cell expansion and cell proliferation.
Functional Keywords
- growth , gibberellin , cell-elongation , breeding , panicle , homeostasis , ga , dwarf , height , plant-height , GA , shoot , resistance , salt , tolerance , salt-tolerance , salt-stress , stress , plant-growth , biomass , Gibberellin , biomass-production , GA-biosynthesis , leaf , grain , grain-size , cell-proliferation , leaf-angle , cell-expansion
Literature and News
- Identification of a 98-kb DNA segment containing the rice Eui gene controlling uppermost internode elongation, and construction of a TAC transgene sublibrary . DOI: 10.1007/s00438-004-1019-3 ; PMID: 15338279
- Gibberellin homeostasis and plant height control by EUI and a role for gibberellin in root gravity responses in rice . DOI: 10.1038/cr.2008.28 ; PMID: 18268540
- Altered disease development in the eui mutants and Eui overexpressors indicates that gibberellins negatively regulate rice basal disease resistance . DOI: 10.1093/mp/ssn021 ; PMID: 19825558
- The APETALA-2-like transcription factor OsAP2-39 controls key interactions between abscisic acid and gibberellin in rice . DOI: 10.1371/journal.pgen.1001098 ; PMID: 20838584
- ELONGATED UPPERMOST INTERNODE encodes a cytochrome P450 monooxygenase that epoxidizes gibberellins in a novel deactivation reaction in rice . DOI: 10.1105/tpc.105.038455 ; PMID: 16399803
- EUI1, encoding a putative cytochrome P450 monooxygenase, regulates internode elongation by modulating gibberellin responses in rice . DOI: 10.1093/pcp/pci233 ; PMID: 16306061
- Fine mapping and in silico isolation of the EUI1 gene controlling upper internode elongation in rice . DOI: 10.1007/s11103-005-2762-5 ; PMID: 16463101
- The Intronic cis Element SE1 Recruits trans-Acting Repressor Complexes to Repress the Expression of ELONGATED UPPERMOST INTERNODE1 in Rice . DOI: 10.1016/j.molp.2018.03.001 ; PMID: 29524649
- OsCYP714D1 improves plant growth and salt tolerance through regulating gibberellin and ion homeostasis in transgenic poplar . DOI: 10.1016/j.plaphy.2021.10.023 ; PMID: 34715569
- LG5, a Novel Allele of EUI1, Regulates Grain Size and Flag Leaf Angle in Rice . DOI: 10.3390/plants12030675 ; PMID: 36771759
Gene Resources
- UniProt: Q5KQH7
- EMBL: AC137619, AK109526, AP008211
- AlphaFoldDB: Q5KQH7
- EnsemblPlants: Os05t0482400-01
- Gramene: Os05t0482400-01
- KEGG: dosa:Os05g0482400
- Orthologous matrix: HPQASFM
- InterPro: IPR001128, IPR002401, IPR017972
- PANTHER: PTHR24282, PTHR24282:SF39
- SUPFAM: SSF48264
- PROSITE: PS00086
- Gene3D: 1.10.630.10
- OrthoDB: Q5KQH7
- SWISS-MODEL: Q5KQH7
- Conserved Domain Database: cd20640
- eggNOG: KOG0157
Sequences
cDNA Sequence
- >LOC_Os05g40384.1
TAAACATATCCGCTAATCCCCCATCCCAGGTGGAATATAAACCCCCGCAAGAGGGAGAAGAAGAAGAAGGGCGAGGTGAGCGAGGCGAGACTCGAGAGAGAGGAGAAGATCCAGAAATCGCAAGCTGCACGCTATAGCTAGGTAGCTATCTCCGGGGAAAGAGAGAGTAGGCTGCCATGGAGAGCTTCTTCGTCTTCTTCACGGCGGCGGCGTTGCCGGTGGTGGTGGCGGCGGCGGTGATCGCCGGGCTGTGCATTACGGCGGCGTGGCTGGCGAGGCCGCGGCGCGTGGCGGAGGTGTTCCGGAGGCAGGGGATCGACGGCCCGCCGCCGTCGTCGTTCCTGGCGGGGAACCTCCCGGAGATGAAGGCGAGGGTGGCCGCCGCGGCGTCGGCGGCGGCGCCAACGGCGGACGGGGAGGAGACCGCCTCCGCCGGCGGCGGCGGCGGTGGCCGGGACTTCGAGAAGGACGGGTTCGACGACTACTGCACCAGGATCTTCCCTTACTTCCACAAGTGGAGGAAAGCCTACGGCGAGACGTACCTGTACTGGCTGCGGCGGCGGCCGGCGCTGTACGTGACGGACCCGGAGCTCATCGGCGAGATCGGGCGGTGCGTGTCGCTCGACATGGGCAAGCCCAAGTACCTCCAGAAAGGCCAGGAGCCACTCTTCGGCGGCGGCGTCCTCAAGGCCAACGGCGCGTGCTGGGCGCGCCAGCGCAAGGTCATCGCGCCGGAGTTCTACATGGCCCGTGTCAGGGCCATGGTCCAGCTCATGGTCGACGCCGCGCAGCCGCTGATCGCCTCCTGGGAATCCAGGATCGACGCCGCTGGAGGCGCGGCGGCGGCGGAGGTCGTCGTCGACGGCGACCTCCGGAGCTTCTCCTTCGATGTGATATCGCGGGCTTGCTTTGGGAGTGATTACTCGAGGGGGAGGGAGATCTTCCTCCGCCTCCGTGAGCTGTCCGGGCTCATGTCGGAGACCAGCGTCATCTTCAGCATCCCTTCGCTGAGGCACCTGCCGACGGGGAAGAACCGGAGGATCTGGAGGCTCACGGGGGAGATCCGGTCGCTGATCATGGAGCTCGTCAGGGAGCGGAGGTGCGCGGCGAGGGCGGCGAGGGAGCACGGCGGGAAGGCGGCGCCGCCGTCGCCGCCGGAGCGCGACTTCCTCGGCTCCATCATCGAGAACAGCGGCGGGCAGCCGCGGCCGGACGACTTCGTGGTGGACAACTGCAAGAACATCTACTTCGCCGGGCACGAGACGAGCGCGGTCACCGCGACGTGGTGCCTCATGCTGCTCGCCGCGCACCCGGAGTGGCAGGACCGCGCCCGCGCCGAGGTGCTCGAGGTCTGCGGCGGCGACGGCGCCGCCGCCCCCGCCGCGCCGGACTTCGACATGGTGTCCCGGATGCGGACGGTGGGGATGGTGGTGCAGGAAACGCTGCGGCTGTTCCCGCCGTCGTCGTTCGTGGTGCGGGAGACGTTCCGGGACATGCAGTTGGGTAGGCTGCTGGCGCCCAAGGGCACCTACCTGTTCGTCCCGGTGTCCACCATGCACCACGACGTCGCCGCCTGGGGCCCGACGGCGAGGCTGTTCGACCCGTCCCGCTTCCGCGACGGCGTGGCGGCGGCGTGCAAGCACCCGCAGGCGTCGTTCATGCCGTTCGGCCTCGGCGCCCGCACCTGCCTCGGCCAGAACCTCGCGCTCGTCGAGGTCAAGACGCTCGTCGCCGTCGTCCTCGCCCGGTTCGAGTTCACGCTCTCGCCGGAGTACAGGCACTCGCCGGCGTTCCGGCTCATCATCGAGCCGGAGTTCGGCCTCCGCCTCCGCATCCGCCGCGCCGGCGGTCAGGACGCCACGTCACAAGTTGACACATCTACTGCACCCGTGCATAGTTCTCATAATTAATTGGCTTCTGTAAACAGCTAGTAAGTCGTGAACTGAAAAAAATACATCACACATACAGAGACAACAGAGGATTACCATTTTACCAGCATACATCCATGGCATACCAAAACAATTTACTAT
CDS Sequence
- >LOC_Os05g40384.1
ATGGAGAGCTTCTTCGTCTTCTTCACGGCGGCGGCGTTGCCGGTGGTGGTGGCGGCGGCGGTGATCGCCGGGCTGTGCATTACGGCGGCGTGGCTGGCGAGGCCGCGGCGCGTGGCGGAGGTGTTCCGGAGGCAGGGGATCGACGGCCCGCCGCCGTCGTCGTTCCTGGCGGGGAACCTCCCGGAGATGAAGGCGAGGGTGGCCGCCGCGGCGTCGGCGGCGGCGCCAACGGCGGACGGGGAGGAGACCGCCTCCGCCGGCGGCGGCGGCGGTGGCCGGGACTTCGAGAAGGACGGGTTCGACGACTACTGCACCAGGATCTTCCCTTACTTCCACAAGTGGAGGAAAGCCTACGGCGAGACGTACCTGTACTGGCTGCGGCGGCGGCCGGCGCTGTACGTGACGGACCCGGAGCTCATCGGCGAGATCGGGCGGTGCGTGTCGCTCGACATGGGCAAGCCCAAGTACCTCCAGAAAGGCCAGGAGCCACTCTTCGGCGGCGGCGTCCTCAAGGCCAACGGCGCGTGCTGGGCGCGCCAGCGCAAGGTCATCGCGCCGGAGTTCTACATGGCCCGTGTCAGGGCCATGGTCCAGCTCATGGTCGACGCCGCGCAGCCGCTGATCGCCTCCTGGGAATCCAGGATCGACGCCGCTGGAGGCGCGGCGGCGGCGGAGGTCGTCGTCGACGGCGACCTCCGGAGCTTCTCCTTCGATGTGATATCGCGGGCTTGCTTTGGGAGTGATTACTCGAGGGGGAGGGAGATCTTCCTCCGCCTCCGTGAGCTGTCCGGGCTCATGTCGGAGACCAGCGTCATCTTCAGCATCCCTTCGCTGAGGCACCTGCCGACGGGGAAGAACCGGAGGATCTGGAGGCTCACGGGGGAGATCCGGTCGCTGATCATGGAGCTCGTCAGGGAGCGGAGGTGCGCGGCGAGGGCGGCGAGGGAGCACGGCGGGAAGGCGGCGCCGCCGTCGCCGCCGGAGCGCGACTTCCTCGGCTCCATCATCGAGAACAGCGGCGGGCAGCCGCGGCCGGACGACTTCGTGGTGGACAACTGCAAGAACATCTACTTCGCCGGGCACGAGACGAGCGCGGTCACCGCGACGTGGTGCCTCATGCTGCTCGCCGCGCACCCGGAGTGGCAGGACCGCGCCCGCGCCGAGGTGCTCGAGGTCTGCGGCGGCGACGGCGCCGCCGCCCCCGCCGCGCCGGACTTCGACATGGTGTCCCGGATGCGGACGGTGGGGATGGTGGTGCAGGAAACGCTGCGGCTGTTCCCGCCGTCGTCGTTCGTGGTGCGGGAGACGTTCCGGGACATGCAGTTGGGTAGGCTGCTGGCGCCCAAGGGCACCTACCTGTTCGTCCCGGTGTCCACCATGCACCACGACGTCGCCGCCTGGGGCCCGACGGCGAGGCTGTTCGACCCGTCCCGCTTCCGCGACGGCGTGGCGGCGGCGTGCAAGCACCCGCAGGCGTCGTTCATGCCGTTCGGCCTCGGCGCCCGCACCTGCCTCGGCCAGAACCTCGCGCTCGTCGAGGTCAAGACGCTCGTCGCCGTCGTCCTCGCCCGGTTCGAGTTCACGCTCTCGCCGGAGTACAGGCACTCGCCGGCGTTCCGGCTCATCATCGAGCCGGAGTTCGGCCTCCGCCTCCGCATCCGCCGCGCCGGCGGTCAGGACGCCACGTCACAAGTTGACACATCTACTGCACCCGTGCATAGTTCTCATAATTAA
Protein Sequence
- >LOC_Os05g40384.1
MESFFVFFTAAALPVVVAAAVIAGLCITAAWLARPRRVAEVFRRQGIDGPPPSSFLAGNLPEMKARVAAAASAAAPTADGEETASAGGGGGGRDFEKDGFDDYCTRIFPYFHKWRKAYGETYLYWLRRRPALYVTDPELIGEIGRCVSLDMGKPKYLQKGQEPLFGGGVLKANGACWARQRKVIAPEFYMARVRAMVQLMVDAAQPLIASWESRIDAAGGAAAAEVVVDGDLRSFSFDVISRACFGSDYSRGREIFLRLRELSGLMSETSVIFSIPSLRHLPTGKNRRIWRLTGEIRSLIMELVRERRCAARAAREHGGKAAPPSPPERDFLGSIIENSGGQPRPDDFVVDNCKNIYFAGHETSAVTATWCLMLLAAHPEWQDRARAEVLEVCGGDGAAAPAAPDFDMVSRMRTVGMVVQETLRLFPPSSFVVRETFRDMQLGRLLAPKGTYLFVPVSTMHHDVAAWGPTARLFDPSRFRDGVAAACKHPQASFMPFGLGARTCLGQNLALVEVKTLVAVVLARFEFTLSPEYRHSPAFRLIIEPEFGLRLRIRRAGGQDATSQVDTSTAPVHSSHN*