Gene Details:

Functional Descriptions:

  • MID1 was primarily expressed in root and leaf vascular tissues, with low level in the tapetum, and was induced by drought and other abiotic stresses.
  • Our findings suggest that MID1 is a transcriptional regulator that promotes rice male development under drought by modulating the expressions of drought-related and anther developmental genes and provide valuable information for crop improvement.
  • MID1 Plays an Important Role in Response to Drought Stress during Reproductive Development.
  • We show here that MID1 (MYB Important for Drought Response1), encoding a putative R-R-type MYB-like transcription factor, can improve rice yield under drought.
  • MID1 was localized to the nucleus and could activate gene expression in yeast, and its homologs were identified in many other plants with high levels sequence similarity.
  • Here, we show that the rice R2R3 MYB transcription factor OsARM1 (ARSENITE-RESPONSIVE MYB1) regulates As-associated transporters genes.
  • Our findings suggest that the OsARM1 transcription factor has essential functions in regulating As uptake and root-to-shoot translocation in rice.
  • Histochemical analysis of OsARM1pro::GUS lines indicated that OsARM1 was expressed in the phloem of vascular bundles in basal and upper nodes.
  • Knockout of OsARM1 (OsARM1-KO CRISPR/Cas9-generated mutants) improved tolerance to As(III) and overexpression of OsARM1 (OsARM1-OE lines) increased sensitivity to As(III).
  • Treatment with As(III) induced OsARM1 transcript accumulation and an OsARM1-GFP fusion localized to the nucleus.
  • In particular, the As(III) levels in node I were significantly higher in OsARM1-KOs, but significantly lower in OsARM1-OEs, compared to wild-type plants, implying that OsARM1 is important for the regulation of root-to-shoot translocation of As.

Literature:

Gene Resources:

  • NCBI ID:
  • UniProt accessions:

Sequences:

cDNA Sequence
  • >LOC_Os05g37060.1
    AGATCCAAGGCTATCTAAGCACCGGCATTTGCATACAGCCGCCTAAGCAAAGAGCACAGCAAGTATACGCGAATCTGAGTGTGTGAGAGAGACTAGTAGCACAGACGAAGGTATGGCGTTCTACCTCGGCAGCATGGGTGGCTCGCCATCGTCATGGGGGGTGGCGGAGGTGCCGGTGCCGAGCAGCAGGCCGTGGAGCAAGGCGGAGGACAAGGTGTTCGAGAGCGCGCTCGTGGCGTTCCCGGAGCACACGCACAACCGGTGGGCGCTCGTCGCGTCGCGGCTCCCGGGGCGCTCGGCGCACGAGGTTTGGGAACACTACCAGGTGCTCGTGGACGATGTCGACCTCATCGAGCGTGGCATGGTTGCGTCCCCGGGCTGCTGGGACGACGACAACAACAGCGCCGGCCACGGCCGTGGCAGTGGTGGGGATGAGCGTCGTCGCGGCGTGCCCTGGACTGAGGAGGAGCACAGGCTATTTCTTGAAGGGCTAGAGAAATATGGGCGTGGCGACTGGCGCAACATCTCGCGCTGGTCGGTGAAGACGCGGACTCCGACGCAAGTGGCGAGCCATGCGCAGAAGTTCTTCATCCGACAGGCCAACGCCAGCAGCCGTGGCGACTCCAAGCGTAAGAGCATCCACGACATCACTGCCCCATGATGTGTGGTCCGATCGAGGATATGCATTATGATCCGGTCCAGTCTAGGTTTAGTTGTATTTCCATCAATTCCAACTAGCTTTCAACCCCATTTCAAAGAGGAGAGAACGAAGGGAGTTTAGTCGTCGGCCCTCCCTGTTAGTTCCTCTCTCTGTGTTGTGTAAAATATAGCTAGGCAATAGCTAAAACTAAGCTTGGCTGGAACCTGAGATCAATTGTTGTGATACTAGTTTGTGTACTTGTCAAATAATATTTGTATCCGTTTAATTTCA
CDS Sequence
  • >LOC_Os05g37060.1
    ATGGCGTTCTACCTCGGCAGCATGGGTGGCTCGCCATCGTCATGGGGGGTGGCGGAGGTGCCGGTGCCGAGCAGCAGGCCGTGGAGCAAGGCGGAGGACAAGGTGTTCGAGAGCGCGCTCGTGGCGTTCCCGGAGCACACGCACAACCGGTGGGCGCTCGTCGCGTCGCGGCTCCCGGGGCGCTCGGCGCACGAGGTTTGGGAACACTACCAGGTGCTCGTGGACGATGTCGACCTCATCGAGCGTGGCATGGTTGCGTCCCCGGGCTGCTGGGACGACGACAACAACAGCGCCGGCCACGGCCGTGGCAGTGGTGGGGATGAGCGTCGTCGCGGCGTGCCCTGGACTGAGGAGGAGCACAGGCTATTTCTTGAAGGGCTAGAGAAATATGGGCGTGGCGACTGGCGCAACATCTCGCGCTGGTCGGTGAAGACGCGGACTCCGACGCAAGTGGCGAGCCATGCGCAGAAGTTCTTCATCCGACAGGCCAACGCCAGCAGCCGTGGCGACTCCAAGCGTAAGAGCATCCACGACATCACTGCCCCATGA
Protein Sequence
  • >LOC_Os05g37060.1
    MAFYLGSMGGSPSSWGVAEVPVPSSRPWSKAEDKVFESALVAFPEHTHNRWALVASRLPGRSAHEVWEHYQVLVDDVDLIERGMVASPGCWDDDNNSAGHGRGSGGDERRRGVPWTEEEHRLFLEGLEKYGRGDWRNISRWSVKTRTPTQVASHAQKFFIRQANASSRGDSKRKSIHDITAP*