Information report for MID1;OsARM1
Gene Details
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Functional Descriptions
- MID1 was primarily expressed in root and leaf vascular tissues, with low level in the tapetum, and was induced by drought and other abiotic stresses.
- Our findings suggest that MID1 is a transcriptional regulator that promotes rice male development under drought by modulating the expressions of drought-related and anther developmental genes and provide valuable information for crop improvement.
- MID1 Plays an Important Role in Response to Drought Stress during Reproductive Development.
- We show here that MID1 (MYB Important for Drought Response1), encoding a putative R-R-type MYB-like transcription factor, can improve rice yield under drought.
- MID1 was localized to the nucleus and could activate gene expression in yeast, and its homologs were identified in many other plants with high levels sequence similarity.
- Here, we show that the rice R2R3 MYB transcription factor OsARM1 (ARSENITE-RESPONSIVE MYB1) regulates As-associated transporters genes.
- Our findings suggest that the OsARM1 transcription factor has essential functions in regulating As uptake and root-to-shoot translocation in rice.
- Histochemical analysis of OsARM1pro::GUS lines indicated that OsARM1 was expressed in the phloem of vascular bundles in basal and upper nodes.
- Knockout of OsARM1 (OsARM1-KO CRISPR/Cas9-generated mutants) improved tolerance to As(III) and overexpression of OsARM1 (OsARM1-OE lines) increased sensitivity to As(III).
- Treatment with As(III) induced OsARM1 transcript accumulation and an OsARM1-GFP fusion localized to the nucleus.
- In particular, the As(III) levels in node I were significantly higher in OsARM1-KOs, but significantly lower in OsARM1-OEs, compared to wild-type plants, implying that OsARM1 is important for the regulation of root-to-shoot translocation of As.
Functional Keywords
- leaf , root , anther , development , drought , transcription-factor , yield , abiotic-stress , reproductive , stress , nucleus , anther-development , biotic-stress , drought-stress , transcriptional-regulator , reproductive-development , vascular-bundle , tolerance , phloem , node
Literature and News
Gene Resources
- UniProt: Q6F2M6
- EMBL: AK107134, AP014961
- AlphaFoldDB: Q6F2M6
- EnsemblPlants: Os05t0442400-01
- Gramene: Os05t0442400-01
- KEGG: osa:4338925
- Orthologous matrix: DDIMAHY
- InterPro: IPR001005, IPR006447, IPR009057
Sequences
cDNA Sequence
- >LOC_Os05g37060.1
AGATCCAAGGCTATCTAAGCACCGGCATTTGCATACAGCCGCCTAAGCAAAGAGCACAGCAAGTATACGCGAATCTGAGTGTGTGAGAGAGACTAGTAGCACAGACGAAGGTATGGCGTTCTACCTCGGCAGCATGGGTGGCTCGCCATCGTCATGGGGGGTGGCGGAGGTGCCGGTGCCGAGCAGCAGGCCGTGGAGCAAGGCGGAGGACAAGGTGTTCGAGAGCGCGCTCGTGGCGTTCCCGGAGCACACGCACAACCGGTGGGCGCTCGTCGCGTCGCGGCTCCCGGGGCGCTCGGCGCACGAGGTTTGGGAACACTACCAGGTGCTCGTGGACGATGTCGACCTCATCGAGCGTGGCATGGTTGCGTCCCCGGGCTGCTGGGACGACGACAACAACAGCGCCGGCCACGGCCGTGGCAGTGGTGGGGATGAGCGTCGTCGCGGCGTGCCCTGGACTGAGGAGGAGCACAGGCTATTTCTTGAAGGGCTAGAGAAATATGGGCGTGGCGACTGGCGCAACATCTCGCGCTGGTCGGTGAAGACGCGGACTCCGACGCAAGTGGCGAGCCATGCGCAGAAGTTCTTCATCCGACAGGCCAACGCCAGCAGCCGTGGCGACTCCAAGCGTAAGAGCATCCACGACATCACTGCCCCATGATGTGTGGTCCGATCGAGGATATGCATTATGATCCGGTCCAGTCTAGGTTTAGTTGTATTTCCATCAATTCCAACTAGCTTTCAACCCCATTTCAAAGAGGAGAGAACGAAGGGAGTTTAGTCGTCGGCCCTCCCTGTTAGTTCCTCTCTCTGTGTTGTGTAAAATATAGCTAGGCAATAGCTAAAACTAAGCTTGGCTGGAACCTGAGATCAATTGTTGTGATACTAGTTTGTGTACTTGTCAAATAATATTTGTATCCGTTTAATTTCA
CDS Sequence
- >LOC_Os05g37060.1
ATGGCGTTCTACCTCGGCAGCATGGGTGGCTCGCCATCGTCATGGGGGGTGGCGGAGGTGCCGGTGCCGAGCAGCAGGCCGTGGAGCAAGGCGGAGGACAAGGTGTTCGAGAGCGCGCTCGTGGCGTTCCCGGAGCACACGCACAACCGGTGGGCGCTCGTCGCGTCGCGGCTCCCGGGGCGCTCGGCGCACGAGGTTTGGGAACACTACCAGGTGCTCGTGGACGATGTCGACCTCATCGAGCGTGGCATGGTTGCGTCCCCGGGCTGCTGGGACGACGACAACAACAGCGCCGGCCACGGCCGTGGCAGTGGTGGGGATGAGCGTCGTCGCGGCGTGCCCTGGACTGAGGAGGAGCACAGGCTATTTCTTGAAGGGCTAGAGAAATATGGGCGTGGCGACTGGCGCAACATCTCGCGCTGGTCGGTGAAGACGCGGACTCCGACGCAAGTGGCGAGCCATGCGCAGAAGTTCTTCATCCGACAGGCCAACGCCAGCAGCCGTGGCGACTCCAAGCGTAAGAGCATCCACGACATCACTGCCCCATGA
Protein Sequence
- >LOC_Os05g37060.1
MAFYLGSMGGSPSSWGVAEVPVPSSRPWSKAEDKVFESALVAFPEHTHNRWALVASRLPGRSAHEVWEHYQVLVDDVDLIERGMVASPGCWDDDNNSAGHGRGSGGDERRRGVPWTEEEHRLFLEGLEKYGRGDWRNISRWSVKTRTPTQVASHAQKFFIRQANASSRGDSKRKSIHDITAP*