Gene Details:

Functional Descriptions:

  • Disruption of OsAKT2 results in delayed growth of rice seedlings under short-day conditions.
  • Here, we identified the rice OsAKT2 as the ortholog of Arabidopsis AtAKT2, which is primarily expressed in the shoot phloem and localized at the plasma membrane.
  • Phosphatidic acid directly binds with rice potassium channel OsAKT2 to inhibit its activity.
  • Multiple basic amino acid residues contribute to phosphatidic acid-mediated inhibition of rice potassium channel OsAKT2.
  • Here, using a PA biosensor (PAleon), we found that the exogenous PA treatment significantly increased the PA level at the plasma membrane of Xenopus oocytes which were used to express OsAKT2 for electrophysiological assays.
  • Rice shaker potassium channel OsAKT2 positively regulates salt tolerance and grain yield by mediating K + redistribution.
  • In addition, OsAKT2 also regulates the translocation of K+ and sucrose from old leaves to young leaves, and affects grain shape and yield.
  • Here, we provide physiological and genetic evidence of the involvement of phloem-localized OsAKT2 in rice salt tolerance.
  • Under salt stress, a T-DNA knock-out mutant, OsAKT2 and two CRISPR lines showed a more sensitive phenotype and higher Na+ accumulation than wild type.
  • Disruption of OsAKT2 decreases K+ concentration in phloem sap and inhibits shoot-to-root redistribution of K+.
  • Additionally, overexpression of OsAKT2 ameliorated the salt tolerance of Arabidopsis.
  • Overexpression of OsAKT2 complemented the K+ deficiency in the phloem sap and leaves of the mutant plants but did not significantly contribute to the transport of sugars.
  • However, the expression of OsAKT2-K191R overcame both the shortage of phloem K+ and sucrose of the akt2 mutant, which was comparable to the effects of the overexpression of AtAKT2, while the expression of the inward mutation AtAKT2-R195K resembled the effects of OsAKT2.

Literature:

Gene Resources:

  • NCBI ID:
  • UniProt accessions:

Sequences:

cDNA Sequence
  • >LOC_Os05g35410.1
    TATGTTTGGTCGTTTTGATTTAATTAACTAATGAAAATGGATCTCATCTTCAGCCTCTCTGCCGACAGTCACCCAAGCAATAAGTTTTTTTTTGGTTTTGAAAAAGAAAAAAATATTGGTCGTTTTGATTATTTGAACACTTAAGTGATGCAGACTTGTTGTGCATTCACAGATATCTGTCGACGTTCTTCATCATGGATGTGGCGTCGACTATCCCGTTCCAAGGCCTTGCCTACATCGTCACCGGCGAGGTCAGGGAGAGCCCGGCGTTCAGCCTCCTCGGCATCCTCCGGCTATGGCGTCTCAGGAAGGTCAAGCAGTTCTTCACAAGGCTGGAGAAGGACATCAGGTTCAACTACTTCTGGATCCGCTGCGCCCGGCTCATCGCGGTGACGCTGTTCCTGGTGCACTGCGCGGGGTGCCTGTACTACCTGATCGCGGACAGGTACCCGCACAGGGAGAAGACGTGGATCGGCGCGGTGATCCCGGACTTCCAGGAGGCGAGCCTGTGGATCCGCTACACCTCCTCCGTCTACTGGTCCATCACCACCATGACCACCGTCGGCTACGGCGACATGCACGCCCAGAACACCGTCGAGATGATCTTCAACATCTTCTACATGCTCTTCAACCTCGGCCTCACCGCCTACCTCATCGGCAACATGACCAACCTCGTCGTTGAGGGCACCCGCCGTACCATGGAATTCAGGAACAGCATTCGTGCTGCCTCCAATTTCGTGGGCCGGAACCACCTGCCGCCTAGGCTGAAGCAGCAGATACTGGCCTACATGTGCCTCAAGTTCAGGGCAGAGAGCCTGAACCAGCAGCAGCTGATGGATCAGCTCCCCAAATCCATCTGCAAGGGCATCTGCGAGTACCTCTTCCTCCCCGTCGTCAAGGACGTCTACCTCTTCAAGGGTGTCTCAAGAGAAGTGCTCCTTCTCATGGTTACGAAGATGAAGCCGGAGTACATACCGCCGAAGGAGGACGTGATCGTGCAGAACGAGGCGCCGGACGACGTCTACATCGTGGTGTCCGGCGAGGTGGAGGTGATCTACTCCGACGGCGAAGCCGAGGAGCGGGTGGTGGCGACGCTGGGGACGAGGGGCGTGTTCGGCGAGGTGAGCGCGCTCAGCGACCGCCCGCAGAGCTTCACGCTGCGGACGCGGACGCTGTGCCAGCTGCTGCGCCTGCGCCAGGCCGCGCTCAAGGAGGCCATGCAGAGCAAGCCCGAGGACAGCGTCGTCATCATCAAGAACTTCCTCAAGCATCAGATCGAGATGCACGACATGAAGGTGGAGGACTTGCTCGGGGAGGACGCCGCCGGCGAGTACGACCACGGCAACATACCGTGCAATCTGCTGACGGTGGCAGCCACCGGGAACAGCAGCTTCCTTGAGGATCTCCTGAAAGTTGGAATGGATCCTGACGTCGGTGACTCCAAGGGAAGAACTGCACTGCATATAGCGGCATCGAAGGGGTACGAGGACTGCGTGCTGGTTCTCCTCAAGCAAGCGTGCAACGTAAACATCAAAGACGCGCAGGGCAACACGGCGCTGTGGAACGCCATCGCCGCGAGGCACCACAAGATCTTCAACATCCTGTACCACTTCGCGCGCGTCTCGAGCCCGCACCACGCCGCCGGCGACCTCCTCTGCCTCGCCGCGCGCCGGGGCGACCTCGACACGCTCCGGGAGCTCCTCAAGCACGGCCTCGCCGTCGACTCGGAGGACCGGGACGGCGCCACGGCGCTCCGCGTCGCGCTCGCCGAGGGCCACGCCGACGTCGCCAGGCTCCTCGTCCTCAACGGCGCCAGCGTCGACAGGGCGGCCAGCCACAACGAGCAGCAGGCGGCGGCGGCGGTGTCGGTCGACGAGCTGCGGGAGCTCATGAAGACGCGCGAGCTCGCGCACCCGGTCACCATCGTCGTCGACTCGCCGTCGCCGGCCGCCGCAGCCGTCATCCGGGAGGTCGGCTCGTCGGGGGACAGCCGGAATGGGCGGCGCCAGAGCGCGAGATCAGACGGCGCGCACTGGCCACGGGTGAGCATATACAGGGGCCATCCATTCGTCAGAAACCGTAGCTCAGAAGCCGGCAAGCTGATCAACCTGCCCGGCACCATGGAAGAATTCAGAATCATCATAGAGGAGAAACTGAAAGTCGACGCGAGGAAGACGCTGATCATGAACGACGAAGGAGCGGAGATTGACTCGATCGACGTGATCCGGGACAACGATAAGCTGTTCATAGTGACCGAGGAGCATATGACAGCAGTAGCATCGATGGACTCGGTGTCTGGATCATAGCATATTCGAGTTTGCTGAATAAGGCCTTGTCCAGCTTGGAGGGATTTGAGTGGGAATGTTTTTGCATCACAATAGGTGTAAAACTAGTCTCCCCAATCTGTTCCTTTTAGGATTAACCGAACAAAGCCTAATCAAGTTTTCTATTGGCGACAATCCAACAAATGTGAGAAGTGCCAATATAATTGAAGTGATTAATGGTTTTGTAGTGTATTGGATTAGGAGATAGATATGCCATAAACAAGATTCACAAATTGCTACTACCTCCGTTTCAAAAATTGTACTCCATCTATTTTATATTATAAAACGTTTTGATTTTTTTT
CDS Sequence
  • >LOC_Os05g35410.1
    ATGGATGTGGCGTCGACTATCCCGTTCCAAGGCCTTGCCTACATCGTCACCGGCGAGGTCAGGGAGAGCCCGGCGTTCAGCCTCCTCGGCATCCTCCGGCTATGGCGTCTCAGGAAGGTCAAGCAGTTCTTCACAAGGCTGGAGAAGGACATCAGGTTCAACTACTTCTGGATCCGCTGCGCCCGGCTCATCGCGGTGACGCTGTTCCTGGTGCACTGCGCGGGGTGCCTGTACTACCTGATCGCGGACAGGTACCCGCACAGGGAGAAGACGTGGATCGGCGCGGTGATCCCGGACTTCCAGGAGGCGAGCCTGTGGATCCGCTACACCTCCTCCGTCTACTGGTCCATCACCACCATGACCACCGTCGGCTACGGCGACATGCACGCCCAGAACACCGTCGAGATGATCTTCAACATCTTCTACATGCTCTTCAACCTCGGCCTCACCGCCTACCTCATCGGCAACATGACCAACCTCGTCGTTGAGGGCACCCGCCGTACCATGGAATTCAGGAACAGCATTCGTGCTGCCTCCAATTTCGTGGGCCGGAACCACCTGCCGCCTAGGCTGAAGCAGCAGATACTGGCCTACATGTGCCTCAAGTTCAGGGCAGAGAGCCTGAACCAGCAGCAGCTGATGGATCAGCTCCCCAAATCCATCTGCAAGGGCATCTGCGAGTACCTCTTCCTCCCCGTCGTCAAGGACGTCTACCTCTTCAAGGGTGTCTCAAGAGAAGTGCTCCTTCTCATGGTTACGAAGATGAAGCCGGAGTACATACCGCCGAAGGAGGACGTGATCGTGCAGAACGAGGCGCCGGACGACGTCTACATCGTGGTGTCCGGCGAGGTGGAGGTGATCTACTCCGACGGCGAAGCCGAGGAGCGGGTGGTGGCGACGCTGGGGACGAGGGGCGTGTTCGGCGAGGTGAGCGCGCTCAGCGACCGCCCGCAGAGCTTCACGCTGCGGACGCGGACGCTGTGCCAGCTGCTGCGCCTGCGCCAGGCCGCGCTCAAGGAGGCCATGCAGAGCAAGCCCGAGGACAGCGTCGTCATCATCAAGAACTTCCTCAAGCATCAGATCGAGATGCACGACATGAAGGTGGAGGACTTGCTCGGGGAGGACGCCGCCGGCGAGTACGACCACGGCAACATACCGTGCAATCTGCTGACGGTGGCAGCCACCGGGAACAGCAGCTTCCTTGAGGATCTCCTGAAAGTTGGAATGGATCCTGACGTCGGTGACTCCAAGGGAAGAACTGCACTGCATATAGCGGCATCGAAGGGGTACGAGGACTGCGTGCTGGTTCTCCTCAAGCAAGCGTGCAACGTAAACATCAAAGACGCGCAGGGCAACACGGCGCTGTGGAACGCCATCGCCGCGAGGCACCACAAGATCTTCAACATCCTGTACCACTTCGCGCGCGTCTCGAGCCCGCACCACGCCGCCGGCGACCTCCTCTGCCTCGCCGCGCGCCGGGGCGACCTCGACACGCTCCGGGAGCTCCTCAAGCACGGCCTCGCCGTCGACTCGGAGGACCGGGACGGCGCCACGGCGCTCCGCGTCGCGCTCGCCGAGGGCCACGCCGACGTCGCCAGGCTCCTCGTCCTCAACGGCGCCAGCGTCGACAGGGCGGCCAGCCACAACGAGCAGCAGGCGGCGGCGGCGGTGTCGGTCGACGAGCTGCGGGAGCTCATGAAGACGCGCGAGCTCGCGCACCCGGTCACCATCGTCGTCGACTCGCCGTCGCCGGCCGCCGCAGCCGTCATCCGGGAGGTCGGCTCGTCGGGGGACAGCCGGAATGGGCGGCGCCAGAGCGCGAGATCAGACGGCGCGCACTGGCCACGGGTGAGCATATACAGGGGCCATCCATTCGTCAGAAACCGTAGCTCAGAAGCCGGCAAGCTGATCAACCTGCCCGGCACCATGGAAGAATTCAGAATCATCATAGAGGAGAAACTGAAAGTCGACGCGAGGAAGACGCTGATCATGAACGACGAAGGAGCGGAGATTGACTCGATCGACGTGATCCGGGACAACGATAAGCTGTTCATAGTGACCGAGGAGCATATGACAGCAGTAGCATCGATGGACTCGGTGTCTGGATCATAG
Protein Sequence
  • >LOC_Os05g35410.1
    MDVASTIPFQGLAYIVTGEVRESPAFSLLGILRLWRLRKVKQFFTRLEKDIRFNYFWIRCARLIAVTLFLVHCAGCLYYLIADRYPHREKTWIGAVIPDFQEASLWIRYTSSVYWSITTMTTVGYGDMHAQNTVEMIFNIFYMLFNLGLTAYLIGNMTNLVVEGTRRTMEFRNSIRAASNFVGRNHLPPRLKQQILAYMCLKFRAESLNQQQLMDQLPKSICKGICEYLFLPVVKDVYLFKGVSREVLLLMVTKMKPEYIPPKEDVIVQNEAPDDVYIVVSGEVEVIYSDGEAEERVVATLGTRGVFGEVSALSDRPQSFTLRTRTLCQLLRLRQAALKEAMQSKPEDSVVIIKNFLKHQIEMHDMKVEDLLGEDAAGEYDHGNIPCNLLTVAATGNSSFLEDLLKVGMDPDVGDSKGRTALHIAASKGYEDCVLVLLKQACNVNIKDAQGNTALWNAIAARHHKIFNILYHFARVSSPHHAAGDLLCLAARRGDLDTLRELLKHGLAVDSEDRDGATALRVALAEGHADVARLLVLNGASVDRAASHNEQQAAAAVSVDELRELMKTRELAHPVTIVVDSPSPAAAAVIREVGSSGDSRNGRRQSARSDGAHWPRVSIYRGHPFVRNRSSEAGKLINLPGTMEEFRIIIEEKLKVDARKTLIMNDEGAEIDSIDVIRDNDKLFIVTEEHMTAVASMDSVSGS*