Gene Details:
- MSU gene ID: LOC_Os05g34460
- RAPdb gene ID: Os05g0417100
- Gene Symbol: TCM5
- Genome: MSU7 , IRGSP-1.0
- Species: Oryza sativa
Functional Descriptions:
- In this study, we reported the first rice deg mutant TCM5 (thermo-sensitive chlorophyll-deficient mutant 5) that has an albino phenotype, defective chloroplasts and could not survive after the 4-5 leaf seedling stage when grown at high temperature (32 °C).
- The TCM5 encodes chloroplast-targeted Deg protease protein which is important for chloroplast development and the maintenance of PSII function and its disruption would lead to a defective chloroplast and affected expression levels of genes associated with chloroplast development and photosynthesis at early rice seedling stage under high temperatures.
- The Rice TCM5 Gene Encoding a Novel Deg Protease Protein is Essential for Chloroplast Development under High Temperatures.
- In TCM5 mutants grown at high temperatures, the transcript levels of certain genes associated with chloroplast development especially PSII-associated genes were severely affected, but recovered to normal levels at low temperatures.
- These results showed important role of TCM5 for chloroplast development under high temperatures.
- Map-based cloning showed that TCM5 encoding a chloroplast-targeted Deg protease protein.
Function-related keywords:
- leaf , temperature , seedling , chloroplast , development , map-based-cloning , photosynthesis , chloroplast-development
Literature:
- The Rice TCM5 Gene Encoding a Novel Deg Protease Protein is Essential for Chloroplast Development under High Temperatures . DOI: 10.1186/s12284-016-0086-5 ; PMID: 27000876
Related News:
Gene Resources:
- NCBI ID: AK073199
- UniProt accessions:
Sequences:
cDNA Sequence
- >LOC_Os05g34460.1
GACCGCCTCCTGCCCCGCGCCGCGCCGCGCCGCTTCTTCCGGCGAGGCCGCCCCGCCACCACCATGCTCGCCTCCGTCCGCTCTCTCCGCCGCCTCTCCTCCTCCTCCTCGTCCTCGTCCTTGTCCCCCGCCTCGCTACGCCGCCTCCTCCTCCTCCTCCACCGCCCGCCGCCTCCGCCCCCGCCCCCTCGGCCTCCTCTCCCGCCCTTCCAAACCCTAGCCCGAGGCATCCTGCCGCGCATCGCGGCGCGGCCTGTGCCCCGCCGCTTCTCCACCGCGTCCTGCTCCTCCACGCTCTTCCGCGTGGGGGAATGCGGCGCCCCCGGCGCCACCGCCATACCCGAGGCGGAGCGAGGCGAGGAGGAGGAGCGTGAGGGGGAGGATGGAGGCGAGGCCGACGCGGCGGTGGAGGTGGCGGCGGGGAGGCATGACACGGACGCGTACGCGGCGGTGGAGCTGGCGCTGGACTCGGTGGTGAAGGTTTTCACGGTGTCCAGTAGCCCCAACTACTTCCTGCCGTGGCAGAACAAGGCGCAGCGCGAGAGCATGGGCTCAGGATTTGTAATTCCGGGGAGGAGAATCATCACAAATGCTCATGTTATAGCGGATCATACCTTTGTTCTCGTGAGGAAGCATGGGTCGCCTACTAAGTACAAGGCAGAAGTCCAGGCTGTTGGCCATGAGTGCGACTTGGCTCTTCTGAAAGTTGATAGTGAGGAGTTTTGGGATGGAATGAACAGTTTGGAGCTTGGAGACATTCCCTTCTTGCAGGAAGCAGTTGCTGTGGTTGGCTACCCGCAAGGGGGGGACAACATTTCTGTCACCAAGGGTGTGGTCTCTAGAGTTGAGCCAACACAATATGCACATGGTGCTACTCAGCTCATGGCCATACAAATAGATGCGGCTATCAACCCAGGAAACAGCGGAGGGCCTGCAATTATGGGCGACAAAGTTGCTGGAGTTGCTTTTCAAAATTTATCAGGGGCAGAAAACATTGGGTATATTATACCGGTGCCTGTAATTAAACGTTTCATCTCTGGAGTGGAAGAGAGCGGTAAATATTCTGGTTTTTGTACCCTTGGAATATCTTGCCAGGCCACAGAGAATATTCAATTAAGAGAGTGCTTTGGGATGAAGCCTGATTTGACTGGAGTGTTAGTGAGTAGAATAAATCCTCTATCTGATGCTCACAGAGTTCTTAAGAAAGATGACATTCTTCTTGAGTTTGACGGTGTGCCTATAGCAAATGATGGCACAGTCCCATTTCGAAATAGGGAGAGAATCACCTTCGATCATCTGGTGTCCATGAAGAAGCCTGGGGAAATGGCGGTTCTCAAAGTTCTAAGAGATGGCAAAGAGCATGAATTGAATGTCACTGTTAGACCTTTGCAACCTTTAGTCCCTGTACATCAATTTGATAAACTACCCAGCTACTACATCTTTGCTGGTTTTGTTTTCATCCCACTGACCCAGCCTTATCTCCATGAATTTGGAGAAGACTGGTACAACAATTCGCCACGCCGACTGTGTGAACGGGCACTGAGAGAGCTTCCAAAAAAAGCTGCTGAACAACTAGTTATCCTATCTCAGGTTCTCATGGATGATATTAATGTTGGTTATGAAAGGCTCGCTGAACTACAGGTAAAAAAGGTGAACGGTGTGGAAGTAGAAAATCTGAAGCATCTGTGCAGCCTTGTGGAAAGCTGCACTGAAGAAAACCTGAGATTTGATCTGGACGATGAGCGGGTGATCGTCCTGAAATATCAGAACGCAAAGCTCGCCACGTCCCGGATCTTAAAACGGCACAGGATACCGTCGGCCATATCTGCTGACCTCGTCGACGAACAGGCGACTGACGACGGTGAGGAGGCGACTAATGGTGAGATCGAGGCATCATGCACTAGCTAACATTGTTTTGCATACTTAGCTAGGCAATTACCTGCGGAAGAATAGATAGATATTAGCACGGTTCAATTTCCAGAGACAGACGCAGTGAGGCGAGGACTGCCCGGAGCACTGCGCTGGTTGCAGCGGCTCCTGTTCGGCCGTCTCAGTTTTGACGTGAGGGGTGGGTGCTCCGGTTTCCCTCAGCAAATAAGAAGTGAAGAACCCAAGTTCTTCAGCCTCATGATATGTTGTAACACAAATTATCTTCCCCATTTCTTCCCAATATTTCTCATTCTTTCACCAAAAGTGCCGTGGCACAGTCACCTCATATTGAACTCAGATTATATATATCATCACGAAACAGAGCATATGAAACAGGAGTTACAAAGTTTACAGCTCGATGAAAATCCACAATAAGTTTCTTATGTACTTTCTTGGTAAATTATCACCCTTGCTGTTAATCCCTCCATTTCATATTAT
CDS Sequence
- >LOC_Os05g34460.1
ATGCTCGCCTCCGTCCGCTCTCTCCGCCGCCTCTCCTCCTCCTCCTCGTCCTCGTCCTTGTCCCCCGCCTCGCTACGCCGCCTCCTCCTCCTCCTCCACCGCCCGCCGCCTCCGCCCCCGCCCCCTCGGCCTCCTCTCCCGCCCTTCCAAACCCTAGCCCGAGGCATCCTGCCGCGCATCGCGGCGCGGCCTGTGCCCCGCCGCTTCTCCACCGCGTCCTGCTCCTCCACGCTCTTCCGCGTGGGGGAATGCGGCGCCCCCGGCGCCACCGCCATACCCGAGGCGGAGCGAGGCGAGGAGGAGGAGCGTGAGGGGGAGGATGGAGGCGAGGCCGACGCGGCGGTGGAGGTGGCGGCGGGGAGGCATGACACGGACGCGTACGCGGCGGTGGAGCTGGCGCTGGACTCGGTGGTGAAGGTTTTCACGGTGTCCAGTAGCCCCAACTACTTCCTGCCGTGGCAGAACAAGGCGCAGCGCGAGAGCATGGGCTCAGGATTTGTAATTCCGGGGAGGAGAATCATCACAAATGCTCATGTTATAGCGGATCATACCTTTGTTCTCGTGAGGAAGCATGGGTCGCCTACTAAGTACAAGGCAGAAGTCCAGGCTGTTGGCCATGAGTGCGACTTGGCTCTTCTGAAAGTTGATAGTGAGGAGTTTTGGGATGGAATGAACAGTTTGGAGCTTGGAGACATTCCCTTCTTGCAGGAAGCAGTTGCTGTGGTTGGCTACCCGCAAGGGGGGGACAACATTTCTGTCACCAAGGGTGTGGTCTCTAGAGTTGAGCCAACACAATATGCACATGGTGCTACTCAGCTCATGGCCATACAAATAGATGCGGCTATCAACCCAGGAAACAGCGGAGGGCCTGCAATTATGGGCGACAAAGTTGCTGGAGTTGCTTTTCAAAATTTATCAGGGGCAGAAAACATTGGGTATATTATACCGGTGCCTGTAATTAAACGTTTCATCTCTGGAGTGGAAGAGAGCGGTAAATATTCTGGTTTTTGTACCCTTGGAATATCTTGCCAGGCCACAGAGAATATTCAATTAAGAGAGTGCTTTGGGATGAAGCCTGATTTGACTGGAGTGTTAGTGAGTAGAATAAATCCTCTATCTGATGCTCACAGAGTTCTTAAGAAAGATGACATTCTTCTTGAGTTTGACGGTGTGCCTATAGCAAATGATGGCACAGTCCCATTTCGAAATAGGGAGAGAATCACCTTCGATCATCTGGTGTCCATGAAGAAGCCTGGGGAAATGGCGGTTCTCAAAGTTCTAAGAGATGGCAAAGAGCATGAATTGAATGTCACTGTTAGACCTTTGCAACCTTTAGTCCCTGTACATCAATTTGATAAACTACCCAGCTACTACATCTTTGCTGGTTTTGTTTTCATCCCACTGACCCAGCCTTATCTCCATGAATTTGGAGAAGACTGGTACAACAATTCGCCACGCCGACTGTGTGAACGGGCACTGAGAGAGCTTCCAAAAAAAGCTGCTGAACAACTAGTTATCCTATCTCAGGTTCTCATGGATGATATTAATGTTGGTTATGAAAGGCTCGCTGAACTACAGGTAAAAAAGGTGAACGGTGTGGAAGTAGAAAATCTGAAGCATCTGTGCAGCCTTGTGGAAAGCTGCACTGAAGAAAACCTGAGATTTGATCTGGACGATGAGCGGGTGATCGTCCTGAAATATCAGAACGCAAAGCTCGCCACGTCCCGGATCTTAAAACGGCACAGGATACCGTCGGCCATATCTGCTGACCTCGTCGACGAACAGGCGACTGACGACGGTGAGGAGGCGACTAATGGTGAGATCGAGGCATCATGCACTAGCTAA
Protein Sequence
- >LOC_Os05g34460.1
MLASVRSLRRLSSSSSSSSLSPASLRRLLLLLHRPPPPPPPPRPPLPPFQTLARGILPRIAARPVPRRFSTASCSSTLFRVGECGAPGATAIPEAERGEEEEREGEDGGEADAAVEVAAGRHDTDAYAAVELALDSVVKVFTVSSSPNYFLPWQNKAQRESMGSGFVIPGRRIITNAHVIADHTFVLVRKHGSPTKYKAEVQAVGHECDLALLKVDSEEFWDGMNSLELGDIPFLQEAVAVVGYPQGGDNISVTKGVVSRVEPTQYAHGATQLMAIQIDAAINPGNSGGPAIMGDKVAGVAFQNLSGAENIGYIIPVPVIKRFISGVEESGKYSGFCTLGISCQATENIQLRECFGMKPDLTGVLVSRINPLSDAHRVLKKDDILLEFDGVPIANDGTVPFRNRERITFDHLVSMKKPGEMAVLKVLRDGKEHELNVTVRPLQPLVPVHQFDKLPSYYIFAGFVFIPLTQPYLHEFGEDWYNNSPRRLCERALRELPKKAAEQLVILSQVLMDDINVGYERLAELQVKKVNGVEVENLKHLCSLVESCTEENLRFDLDDERVIVLKYQNAKLATSRILKRHRIPSAISADLVDEQATDDGEEATNGEIEASCTS*