Gene Details:
- MSU gene ID: LOC_Os05g31040
- RAPdb gene ID: Os05g0374200
- Gene Symbol: OsCKX9
- Genome: MSU7 , IRGSP-1.0
- Species: Oryza sativa
Functional Descriptions:
- Here we show that the cytokinin (CK) content is dramatically increased in shoot bases of the rice SL signaling mutant d53 By examining transcript levels of all the CK metabolism-related genes after treatment with SL analog GR24, we identified CYTOKININ OXIDASE/DEHYDROGENASE 9 (OsCKX9) as a primary response gene significantly up-regulated within 1 h of treatment in the wild type but not in d53 We also found that OsCKX9 functions as a cytosolic and nuclear dual-localized CK catabolic enzyme, and that the overexpression of OsCKX9 suppresses the browning of d53 calli.
- Both the CRISPR/Cas9-generated OsCKX9 mutants and OsCKX9-overexpressing transgenic plants showed significant increases in tiller number and decreases in plant height and panicle size, suggesting that the homeostasis of OsCKX9 plays a critical role in regulating rice shoot architecture.
Function-related keywords:
- shoot , panicle , tiller , cytokinin , architecture , homeostasis , height , plant-height , panicle-size , tiller-number , shoot-architecture
Literature:
- Strigolactone promotes cytokinin degradation through transcriptional activation of CYTOKININ OXIDASE/DEHYDROGENASE 9 in rice . DOI: 10.1073/pnas.1810980116 ; PMID: 31235564
Related News:
Gene Resources:
Sequences:
cDNA Sequence
- >LOC_Os05g31040.1
ATGAGGCCATCACTCTTGCAGTATCTGAAGCTGCTCCTGTTACTTGCCCTTGGTGGTGTCACCACCATGCATGTGCCTAAACAAGATGTGCCATCATCCCTGGAGGAACTCACCCTTGATGGCCATTTCAGCTTCCACGATGTGTCTGCTGCTGCACAGGACTTTGGCAACCTGTCCAGCTTCCCGCCGGTTGCGGTGCTTCATCCTGGCTCAGTGGCTGACATTGCCACAACCATCAGGCATGTGTTCTTGATGGGTGAGCACTCCACGCTCACAGTGGCAGCCCGTGGACATGGGCACTCGCTGTATGGGCAGTCCCAGGCTGCTGAGGGAATCATCATCAGTATGGAATCCCTCCAGAGTAACACAATGAGGGTGAACCCTGGCGTATCACCCTATGTCGATGCTTCAGGAGGAGAACTCTGGATAAATGTTTTGCATGAGACTCTGAAGTATGGTTTAGCACCAAAGTCGTGGACGGACTATCTCCATCTCACGGTTGGAGGCACACTGTCGAATGCAGGAGTCAGTGGGCAGACCTTCCGGCATGGCCCACAGATCAGCAATGTCAACGAGCTGGAAATTGTGACTGGAAGAGGTGATGTTATCACATGCTCACCTGAACAAAACTCTGATCTCTTCCATGCTGCCCTTGGTGGCCTAGGTCAGTTTGGCGTCATCACAAGAGCCAGGATTCCTCTTGAACCTGCTCCAAAAATGGTAAGGTGGCTACGAGTTCTCTACTTGGATTTCACAAGCTTCACCGAAGACCAGGAAATGCTCATTTCAGCAGAGAAGACATTTGACTACATCGAGGGCTTTGTCATCATAAATAGGACAGGCATCCTGAACAACTGGAGGTCGTCCTTCAATCCGCAGGACCCAGTGCGTTCTAGCCAGTTTGAATCAGATGGAAAAGTGCTCTTCTGCCTTGAGATGACAAAGAACTTCAACCCTGACGAGGCTGATGTCATGGAACAGGAGGTCAACACACTGCTGTCTCAACTTAGATATATGCCTTCCTCCTTATTCCACACGGATGTAACATACATAGAGTTCTTGGATAGGGTACACTCTTCTGAGATGAAGTTGAGAGCTAAGGGCATGTGGGAAGTCCCACACCCATGGCTTAATATCATCATTCCAAGAAGCATGATCCATAAATTTGCGAAGGAGGTCTTTGGGAAAATCCTGAAAGACAGTAACAATGGTCCCATATTACTCTATCCAGTGAACAAATCCAGATGGGACAACAGAACATCAGTAGTCATACCAGATGAGGAAGTTTTCTACCTGGTGGCATTCCTATCCTCAGCACTTGGCCCCCATAACATCAAACATACATTGGACCTGAACTACAGGATAATAGAGTTCTCTGACAAGGCAGGTATTGGAGTGAAGCAGTATCTCCCAAACTATACCACAGAACAGGAGTGGCAGTCCCATTTTGGTGCAAGGTGGGACACATTTCAACAGAGGAAAAAAGCCTATGACCCCTTGGCCATCTTAGCTCCAGGACAAAGAATATTTCAAAAGGCATCAGCGTCACTACCCTTGCCCTCATGACAGTATCTAATGTAAATATTTATTTCCTGGGAGCAAACGTTTGTAGTTTGTACAGAACTGTAGGTAAAATTTCTTTTACAAAAGGCAATTAACTGTAGCATGGGCAACACAGTTTACTAACTACTGAATATCAGCAGTGAACTAGTGGTGCACTCATGAACATTCTCATCAAGAAGATTAAAGGAGAGCAGCATTGCCTTACTTCCAATTTTCTATGAAGCTCTTTGGAAGCAATAA
CDS Sequence
- >LOC_Os05g31040.1
ATGAGGCCATCACTCTTGCAGTATCTGAAGCTGCTCCTGTTACTTGCCCTTGGTGGTGTCACCACCATGCATGTGCCTAAACAAGATGTGCCATCATCCCTGGAGGAACTCACCCTTGATGGCCATTTCAGCTTCCACGATGTGTCTGCTGCTGCACAGGACTTTGGCAACCTGTCCAGCTTCCCGCCGGTTGCGGTGCTTCATCCTGGCTCAGTGGCTGACATTGCCACAACCATCAGGCATGTGTTCTTGATGGGTGAGCACTCCACGCTCACAGTGGCAGCCCGTGGACATGGGCACTCGCTGTATGGGCAGTCCCAGGCTGCTGAGGGAATCATCATCAGTATGGAATCCCTCCAGAGTAACACAATGAGGGTGAACCCTGGCGTATCACCCTATGTCGATGCTTCAGGAGGAGAACTCTGGATAAATGTTTTGCATGAGACTCTGAAGTATGGTTTAGCACCAAAGTCGTGGACGGACTATCTCCATCTCACGGTTGGAGGCACACTGTCGAATGCAGGAGTCAGTGGGCAGACCTTCCGGCATGGCCCACAGATCAGCAATGTCAACGAGCTGGAAATTGTGACTGGAAGAGGTGATGTTATCACATGCTCACCTGAACAAAACTCTGATCTCTTCCATGCTGCCCTTGGTGGCCTAGGTCAGTTTGGCGTCATCACAAGAGCCAGGATTCCTCTTGAACCTGCTCCAAAAATGGTAAGGTGGCTACGAGTTCTCTACTTGGATTTCACAAGCTTCACCGAAGACCAGGAAATGCTCATTTCAGCAGAGAAGACATTTGACTACATCGAGGGCTTTGTCATCATAAATAGGACAGGCATCCTGAACAACTGGAGGTCGTCCTTCAATCCGCAGGACCCAGTGCGTTCTAGCCAGTTTGAATCAGATGGAAAAGTGCTCTTCTGCCTTGAGATGACAAAGAACTTCAACCCTGACGAGGCTGATGTCATGGAACAGGAGGTCAACACACTGCTGTCTCAACTTAGATATATGCCTTCCTCCTTATTCCACACGGATGTAACATACATAGAGTTCTTGGATAGGGTACACTCTTCTGAGATGAAGTTGAGAGCTAAGGGCATGTGGGAAGTCCCACACCCATGGCTTAATATCATCATTCCAAGAAGCATGATCCATAAATTTGCGAAGGAGGTCTTTGGGAAAATCCTGAAAGACAGTAACAATGGTCCCATATTACTCTATCCAGTGAACAAATCCAGATGGGACAACAGAACATCAGTAGTCATACCAGATGAGGAAGTTTTCTACCTGGTGGCATTCCTATCCTCAGCACTTGGCCCCCATAACATCAAACATACATTGGACCTGAACTACAGGATAATAGAGTTCTCTGACAAGGCAGGTATTGGAGTGAAGCAGTATCTCCCAAACTATACCACAGAACAGGAGTGGCAGTCCCATTTTGGTGCAAGGTGGGACACATTTCAACAGAGGAAAAAAGCCTATGACCCCTTGGCCATCTTAGCTCCAGGACAAAGAATATTTCAAAAGGCATCAGCGTCACTACCCTTGCCCTCATGA
Protein Sequence
- >LOC_Os05g31040.1
MRPSLLQYLKLLLLLALGGVTTMHVPKQDVPSSLEELTLDGHFSFHDVSAAAQDFGNLSSFPPVAVLHPGSVADIATTIRHVFLMGEHSTLTVAARGHGHSLYGQSQAAEGIIISMESLQSNTMRVNPGVSPYVDASGGELWINVLHETLKYGLAPKSWTDYLHLTVGGTLSNAGVSGQTFRHGPQISNVNELEIVTGRGDVITCSPEQNSDLFHAALGGLGQFGVITRARIPLEPAPKMVRWLRVLYLDFTSFTEDQEMLISAEKTFDYIEGFVIINRTGILNNWRSSFNPQDPVRSSQFESDGKVLFCLEMTKNFNPDEADVMEQEVNTLLSQLRYMPSSLFHTDVTYIEFLDRVHSSEMKLRAKGMWEVPHPWLNIIIPRSMIHKFAKEVFGKILKDSNNGPILLYPVNKSRWDNRTSVVIPDEEVFYLVAFLSSALGPHNIKHTLDLNYRIIEFSDKAGIGVKQYLPNYTTEQEWQSHFGARWDTFQQRKKAYDPLAILAPGQRIFQKASASLPLPS*