Gene Details:

Functional Descriptions:

  • Transgenic rice plants expressing OsPYL/RCAR5, a PYL/RCAR orthologue of rice, were found to be hypersensitive to ABA during seed germination and early seedling growth.
  • A rice orthologue of the ABA receptor, OsPYL/RCAR5, is a positive regulator of the ABA signal transduction pathway in seed germination and early seedling growth.
  • The constitutive expression of OsPYL/RCAR5 in rice driven by the Zea mays ubiquitin promoter induced the expression of many stress-responsive genes even under normal growth conditions and resulted in improved drought and salt stress tolerance in rice.
  • A rice ABA signalling unit composed of OsPYL/RCAR5, OsPP2C30, SAPK2, and OREB1 for ABA-dependent gene regulation was further identified, via interaction assays and a transient gene expression assay.
  • This suggests that, although exogenous expression of OsPYL/RCAR5 is able to improve abiotic stress tolerance in rice, fine regulation of its expression will be required to avoid deleterious effects on agricultural traits.
  • OsPYL/RCARs thus are considered to be good candidate genes for improvement of abiotic stress tolerance in crops.
  • This work demonstrates that the cytosolic ABA receptor OsPYL/RCAR5 in O.
  • Previous work identified OsPYL/RCARs as functional ABA receptors regulating ABA-dependent gene expression in Oryza sativa.

Literature:

Gene Resources:

Sequences:

cDNA Sequence
  • >LOC_Os05g12260.1
    CAGATTTTTGTACCCGCCCGTGTGTGTGCGTGTGTAAGCAAACCCAACCCACCGCTACCCTCTTCTCGCCGCAAGCGAGGCGAGCCAAGCAAACGCGAGGCCGACGACGACGACGAGGAGGAGGAAGCGCAGCCACTAGTCGCGGCGGCGGCGGCGGCGGCGGCGGCGGGTGATCGGAGGAGGGATGGTGGGGCTTGTGGGAGGAGGAGGTTGGAGGGTCGGGGATGATGCGGCGGGTGGGGGTGGGGGAGGAGCGGTGGCGGCGGGGGCTGCGGCGGCGGCGGAGGCGGAGCACATGCGGAGGCTCCACAGCCACGCCCCCGGCGAGCACCAGTGCAGCTCCGCGCTCGTCAAGCACATCAAGGCTCCTGTTCACCTCGTGTGGTCGCTGGTGCGGAGCTTCGACCAGCCGCAGAGGTACAAGCCGTTCGTCAGCCGCTGCGTCGTGCGCGGCGGCGACCTCGAGATCGGCAGCGTGCGCGAGGTCAACGTCAAGACCGGCCTCCCGGCGACCACCAGCACGGAGAGGCTCGAGCTGCTCGACGACGACGAGCACATCCTCAGCGTCAAGTTCGTCGGCGGCGACCACCGCCTCAGGAACTACTCATCCATCGTAACTGTCCATCCGGAGAGCATCGATGGAAGACCAGGGACGCTTGTGATTGAATCATTTGTGGTGGACGTGCCTGATGGAAATACAAAGGACGAGACATGCTACTTTGTCGAGGCCGTGATCAAGTGCAACTTAACATCTCTCGCCGAGGTATCAGAGCGGCTAGCAGTTCAGTCACCCACCTCGCCACTTGAACAGTAGGGTCATCACTGGCACGGGACATGCGAACTGGCATGAATAAGAAGCTTCAAATGGTTGCTAGAGATTTAAATCTTTTTGAGATGGTAGTTCCTCCATTGTCTTCGATATATATGCCTCTTGTAAGGATTAGTATGTAGGGTTAATTATCTTTAGTTGCTGGCTAGTGATGGGCTTAGGTCTTTTTAGGGACTGCACCTAGCTACCTTGTAGTTATGTATGCATACTGCTAGTAGTATGTGTATAAATAGGGAAGCATGGAGTGGTTTTTGCCTCTTTGGTGCACATGTGAAACAACCTCAAGAGTTCTTTTTCCTGCATTTGAGCTGGCTTCCCATTGTAGTATAGTATTGTTGTGTTCTTGGATGACATTCCGCTAACACTTGGGCTTGGTTGGGGGCCTTTTGATTTGGGTGGATCTTGGATTGTGCTGTAATATATACATATGAAAGGAGTTCTCCTTTGCACTATGAAATCCTTTCATAGTCAAAGAATATGCTTATTGAACCAGTATAATATTCCTGC
CDS Sequence
  • >LOC_Os05g12260.1
    ATGGTGGGGCTTGTGGGAGGAGGAGGTTGGAGGGTCGGGGATGATGCGGCGGGTGGGGGTGGGGGAGGAGCGGTGGCGGCGGGGGCTGCGGCGGCGGCGGAGGCGGAGCACATGCGGAGGCTCCACAGCCACGCCCCCGGCGAGCACCAGTGCAGCTCCGCGCTCGTCAAGCACATCAAGGCTCCTGTTCACCTCGTGTGGTCGCTGGTGCGGAGCTTCGACCAGCCGCAGAGGTACAAGCCGTTCGTCAGCCGCTGCGTCGTGCGCGGCGGCGACCTCGAGATCGGCAGCGTGCGCGAGGTCAACGTCAAGACCGGCCTCCCGGCGACCACCAGCACGGAGAGGCTCGAGCTGCTCGACGACGACGAGCACATCCTCAGCGTCAAGTTCGTCGGCGGCGACCACCGCCTCAGGAACTACTCATCCATCGTAACTGTCCATCCGGAGAGCATCGATGGAAGACCAGGGACGCTTGTGATTGAATCATTTGTGGTGGACGTGCCTGATGGAAATACAAAGGACGAGACATGCTACTTTGTCGAGGCCGTGATCAAGTGCAACTTAACATCTCTCGCCGAGGTATCAGAGCGGCTAGCAGTTCAGTCACCCACCTCGCCACTTGAACAGTAG
Protein Sequence
  • >LOC_Os05g12260.1
    MVGLVGGGGWRVGDDAAGGGGGGAVAAGAAAAAEAEHMRRLHSHAPGEHQCSSALVKHIKAPVHLVWSLVRSFDQPQRYKPFVSRCVVRGGDLEIGSVREVNVKTGLPATTSTERLELLDDDEHILSVKFVGGDHRLRNYSSIVTVHPESIDGRPGTLVIESFVVDVPDGNTKDETCYFVEAVIKCNLTSLAEVSERLAVQSPTSPLEQ*