Gene Details:

Functional Descriptions:

  • Overexpression of GSK2 (Go) led to plants with typical BR loss-of-function phenotypes, and suppression of GSK2 resulted in enhanced BR signaling phenotypes.
  • Suppression of DLT can enhance the phenotypes of BR receptor mutant d61-1, and overexpression of DLT obviously suppressed the BR loss-of-function phenotypes of both d61-1 and Go, suggesting that DLT functions downstream of GSK2 to modulate BR responses.
  • These results demonstrate that DLT is a GSK2 substrate, further reinforcing that the BIN2/GSK2 kinase has multiple substrates that carry out various BR responses.
  • Together, these results suggest that OsGSK2 is selected to regulate mesocotyl length by coordinating strigolactone and brassinosteroid signaling during domestication.
  • Variations in the coding region of OsGSK2 alter its kinase activity.
  • In addition, the stability of OsPUB24 was downregulated by BL and bikinin, an inhibitor of OsSK22 (Oryza sativa Shaggy/GSK3-like kinase 22).
  • OsGSK2 physically interacts with OsMYC2, resulting in the degradation of OsMYC2 by phosphorylation and reduces JA-mediated defense to facilitate virus infection.
  • Brassinosteroid treatment increased the LR number, as did mutation of GSK3/SHAGGY-like kinase 2 (OsGSK2), whereas overexpression of OsGSK2 decreased the LR number.
  • OsGSK2 deacetylation attenuated the interaction between OsGSK2 and BRASSINAZOLE RESISTANT 1 (OsBZR1), leading to accumulation of OsBZR1.
  • In addition, inhibition of BR synthesis promotes GSK2 accumulation and suppresses TUD1 stability.
  • Furthermore, the GSK2 degradation process is largely impaired in tud1 in response to BR.
  • In conclusion, our study demonstrates the role of TUD1 in BR-induced GSK2 degradation, thereby advancing our understanding of a critical step in the BR signaling pathway of rice.
  • The U-box ubiquitin ligase TUD1 promotes brassinosteroid-induced GSK2 degradation in rice.
  • Together, our results suggest that OsBSK1-1 may serve as a scaffold protein directly bridging OsBRI1 and OsGSK2 to positively regulate BR signalling, thus affecting plant architecture and grain size in rice.

Literature:

Gene Resources:

Sequences:

cDNA Sequence
  • >LOC_Os05g11730.1
    ATCCCTCCCAGTATAAGCCAAACCAATCCATAGAGGAGGAAGAGGAGGACCAGGTGGTGGCACACCTAAGCTTTGTGCAGTGCCATTCACGCACCTGCAGCTTCCAGCTTTGCCACATGGACCAGCCGGCGCCGGCGCCGGAGCCGATGCTGCTCGACGCGCAGCCGCCCGCCGCCGTCGCCTGCGACAAGAAGCAGCAGGAGGGGGAGGCGCCGTACGCGGAGGGGAATGACGCGGTGACCGGGCACATCATCTCCACCACCATCGGGGGCAAGAACGGCGAGCCCAAGAGGACAATTAGCTACATGGCGGAGCGCGTTGTGGGCACTGGTTCTTTCGGTATCGTCTTTCAGGCTAAATGCTTGGAGACAGGAGAGACTGTTGCCATTAAGAAGGTATTGCAGGACCGACGGTACAAGAACCGTGAGCTTCAGCTTATGCGCGCCATGGACCACCCCAATGTCATCTCCCTGAAGCATTGCTTCTTCTCAACCACAAGTAGGGATGAGCTGTTCCTCAATCTTGTCATGGAATATGTTCCAGAAACACTCTACCGTGTGCTTAAGCACTACAGCAATGCCAACCACCGGATGCCACTTATCTACGTCAAGCTTTACATGTATCAGTTATTTAGGGGGCTTGCGTACATTCATACTGTTCCAGGGGTCTGTCATAGGGATGTGAAGCCACAAAATGTTTTGGTGGATCCTCTAACTCATCAAGTCAAGCTCTGTGACTTTGGGAGCGCAAAAACACTGGTCCCAGGTGAACCCAATATATCATATATATGCTCACGCTACTACCGAGCACCGGAGCTCATATTTGGTGCAACTGAATATACTACATCAATAGATATATGGTCAGCTGGGTGTGTTCTTGCAGAGCTACTCCTTGGTCAGCCATTGTTTCCAGGGGAGAGTGCAGTCGATCAGCTTGTAGAGATAATTAAGGTTCTTGGTACACCAACCCGTGAGGAAATACGTTGCATGAACCCGAACTATACAGAGTTTAGGTTTCCACAGATAAAAGCTCACCCTTGGCACAAGGTTTTCCACAAGAGGATGCCTCCTGAAGCAATAGACCTCGCTTCACGCCTTCTTCAATATTCACCGAGTCTCCGCTGCACTGCTCTTGATGCATGTGCACATCCTTTCTTTGATGAGCTGCGAGAGCCGAATGCACGCTTGCCAAACGGACGTCCATTTCCACCACTATTCAACTTCAAACACGAACTAGCAAATTCTTCTCAAGAGCTCATCAGCAGGCTCATACCAGAACATGTTCGACGGCAAGCTACCCACAACTTCTTCAATACTGGGAGCTAAAAATGCTAAATGCACACCACCAGACCTTTTGTTGGATCGTTTTCGCGGAACCGGTGAAGTTCACATGAAGGCTGAGTCAGATGATTCTTCGAATCCCCGCAAAACAAGAAGAATAGAAAATATGATTCCTCAGATGATGATATGCAAATGCTTCGTTGGAAGTTCAATTCAATCATCGAAGAAGAACAACATTGTAAATCGAGAAGTTTTTGCATCGCGAGTTTGGTAGTGAAACCGGGATCAGCTGGTATGACGGAGGAAACCGAAATGTTTAGATCCATGACTGAGTTTTCTTTCATTTTTTTTGCCCAATTGTAACAGAAGAATATAGTTCCCTAATGTAGGCGTAGTTGTAACCTGTAAACTGCCACTGTTTTGTTCACATTCCATGATGTAAATGCCACCATGCCTCTGATGAATAACTCTCCTTGTAACCTTGTTCCTTCCATCCTTGACTGTTTACCTTAAAGCCGTGGACAGTGTACACTGTACATGTACCGTGCTACACGGAAGGACATATTTGAATTTTTTTTCTCTC
CDS Sequence
  • >LOC_Os05g11730.1
    ATGGACCAGCCGGCGCCGGCGCCGGAGCCGATGCTGCTCGACGCGCAGCCGCCCGCCGCCGTCGCCTGCGACAAGAAGCAGCAGGAGGGGGAGGCGCCGTACGCGGAGGGGAATGACGCGGTGACCGGGCACATCATCTCCACCACCATCGGGGGCAAGAACGGCGAGCCCAAGAGGACAATTAGCTACATGGCGGAGCGCGTTGTGGGCACTGGTTCTTTCGGTATCGTCTTTCAGGCTAAATGCTTGGAGACAGGAGAGACTGTTGCCATTAAGAAGGTATTGCAGGACCGACGGTACAAGAACCGTGAGCTTCAGCTTATGCGCGCCATGGACCACCCCAATGTCATCTCCCTGAAGCATTGCTTCTTCTCAACCACAAGTAGGGATGAGCTGTTCCTCAATCTTGTCATGGAATATGTTCCAGAAACACTCTACCGTGTGCTTAAGCACTACAGCAATGCCAACCACCGGATGCCACTTATCTACGTCAAGCTTTACATGTATCAGTTATTTAGGGGGCTTGCGTACATTCATACTGTTCCAGGGGTCTGTCATAGGGATGTGAAGCCACAAAATGTTTTGGTGGATCCTCTAACTCATCAAGTCAAGCTCTGTGACTTTGGGAGCGCAAAAACACTGGTCCCAGGTGAACCCAATATATCATATATATGCTCACGCTACTACCGAGCACCGGAGCTCATATTTGGTGCAACTGAATATACTACATCAATAGATATATGGTCAGCTGGGTGTGTTCTTGCAGAGCTACTCCTTGGTCAGCCATTGTTTCCAGGGGAGAGTGCAGTCGATCAGCTTGTAGAGATAATTAAGGTTCTTGGTACACCAACCCGTGAGGAAATACGTTGCATGAACCCGAACTATACAGAGTTTAGGTTTCCACAGATAAAAGCTCACCCTTGGCACAAGGTTTTCCACAAGAGGATGCCTCCTGAAGCAATAGACCTCGCTTCACGCCTTCTTCAATATTCACCGAGTCTCCGCTGCACTGCTCTTGATGCATGTGCACATCCTTTCTTTGATGAGCTGCGAGAGCCGAATGCACGCTTGCCAAACGGACGTCCATTTCCACCACTATTCAACTTCAAACACGAACTAGCAAATTCTTCTCAAGAGCTCATCAGCAGGCTCATACCAGAACATGTTCGACGGCAAGCTACCCACAACTTCTTCAATACTGGGAGCTAA
Protein Sequence
  • >LOC_Os05g11730.1
    MDQPAPAPEPMLLDAQPPAAVACDKKQQEGEAPYAEGNDAVTGHIISTTIGGKNGEPKRTISYMAERVVGTGSFGIVFQAKCLETGETVAIKKVLQDRRYKNRELQLMRAMDHPNVISLKHCFFSTTSRDELFLNLVMEYVPETLYRVLKHYSNANHRMPLIYVKLYMYQLFRGLAYIHTVPGVCHRDVKPQNVLVDPLTHQVKLCDFGSAKTLVPGEPNISYICSRYYRAPELIFGATEYTTSIDIWSAGCVLAELLLGQPLFPGESAVDQLVEIIKVLGTPTREEIRCMNPNYTEFRFPQIKAHPWHKVFHKRMPPEAIDLASRLLQYSPSLRCTALDACAHPFFDELREPNARLPNGRPFPPLFNFKHELANSSQELISRLIPEHVRRQATHNFFNTGS*