GSK2;OsGSK2

Gene Details

MSU gene ID: LOC_Os05g11730
RAPdb gene ID: Os05g0207500
Gene Symbol: GSK2 OsGSK2 OsSK22
Genome: MSU7 , IRGSP-1.0
Species: Oryza sativa

Functional Descriptions

Overexpression of GSK2 (Go) led to plants with typical BR loss-of-function phenotypes, and suppression of GSK2 resulted in enhanced BR signaling phenotypes.
Suppression of DLT can enhance the phenotypes of BR receptor mutant d61-1, and overexpression of DLT obviously suppressed the BR loss-of-function phenotypes of both d61-1 and Go, suggesting that DLT functions downstream of GSK2 to modulate BR responses.
These results demonstrate that DLT is a GSK2 substrate, further reinforcing that the BIN2/GSK2 kinase has multiple substrates that carry out various BR responses.
Together, these results suggest that OsGSK2 is selected to regulate mesocotyl length by coordinating strigolactone and brassinosteroid signaling during domestication.
Variations in the coding region of OsGSK2 alter its kinase activity.
In addition, the stability of OsPUB24 was downregulated by BL and bikinin, an inhibitor of OsSK22 (Oryza sativa Shaggy/GSK3-like kinase 22).
OsGSK2 physically interacts with OsMYC2, resulting in the degradation of OsMYC2 by phosphorylation and reduces JA-mediated defense to facilitate virus infection.
Brassinosteroid treatment increased the LR number, as did mutation of GSK3/SHAGGY-like kinase 2 (OsGSK2), whereas overexpression of OsGSK2 decreased the LR number.
OsGSK2 deacetylation attenuated the interaction between OsGSK2 and BRASSINAZOLE RESISTANT 1 (OsBZR1), leading to accumulation of OsBZR1.
In addition, inhibition of BR synthesis promotes GSK2 accumulation and suppresses TUD1 stability.
Furthermore, the GSK2 degradation process is largely impaired in tud1 in response to BR.
In conclusion, our study demonstrates the role of TUD1 in BR-induced GSK2 degradation, thereby advancing our understanding of a critical step in the BR signaling pathway of rice.
The U-box ubiquitin ligase TUD1 promotes brassinosteroid-induced GSK2 degradation in rice.
Together, our results suggest that OsBSK1-1 may serve as a scaffold protein directly bridging OsBRI1 and OsGSK2 to positively regulate BR signalling, thus affecting plant architecture and grain size in rice.

Functional Keywords

Literature and News

Gene Resources

Sequences

cDNA Sequence

>LOC_Os05g11730.1
ATCCCTCCCAGTATAAGCCAAACCAATCCATAGAGGAGGAAGAGGAGGACCAGGTGGTGGCACACCTAAGCTTTGTGCAGTGCCATTCACGCACCTGCAGCTTCCAGCTTTGCCACATGGACCAGCCGGCGCCGGCGCCGGAGCCGATGCTGCTCGACGCGCAGCCGCCCGCCGCCGTCGCCTGCGACAAGAAGCAGCAGGAGGGGGAGGCGCCGTACGCGGAGGGGAATGACGCGGTGACCGGGCACATCATCTCCACCACCATCGGGGGCAAGAACGGCGAGCCCAAGAGGACAATTAGCTACATGGCGGAGCGCGTTGTGGGCACTGGTTCTTTCGGTATCGTCTTTCAGGCTAAATGCTTGGAGACAGGAGAGACTGTTGCCATTAAGAAGGTATTGCAGGACCGACGGTACAAGAACCGTGAGCTTCAGCTTATGCGCGCCATGGACCACCCCAATGTCATCTCCCTGAAGCATTGCTTCTTCTCAACCACAAGTAGGGATGAGCTGTTCCTCAATCTTGTCATGGAATATGTTCCAGAAACACTCTACCGTGTGCTTAAGCACTACAGCAATGCCAACCACCGGATGCCACTTATCTACGTCAAGCTTTACATGTATCAGTTATTTAGGGGGCTTGCGTACATTCATACTGTTCCAGGGGTCTGTCATAGGGATGTGAAGCCACAAAATGTTTTGGTGGATCCTCTAACTCATCAAGTCAAGCTCTGTGACTTTGGGAGCGCAAAAACACTGGTCCCAGGTGAACCCAATATATCATATATATGCTCACGCTACTACCGAGCACCGGAGCTCATATTTGGTGCAACTGAATATACTACATCAATAGATATATGGTCAGCTGGGTGTGTTCTTGCAGAGCTACTCCTTGGTCAGCCATTGTTTCCAGGGGAGAGTGCAGTCGATCAGCTTGTAGAGATAATTAAGGTTCTTGGTACACCAACCCGTGAGGAAATACGTTGCATGAACCCGAACTATACAGAGTTTAGGTTTCCACAGATAAAAGCTCACCCTTGGCACAAGGTTTTCCACAAGAGGATGCCTCCTGAAGCAATAGACCTCGCTTCACGCCTTCTTCAATATTCACCGAGTCTCCGCTGCACTGCTCTTGATGCATGTGCACATCCTTTCTTTGATGAGCTGCGAGAGCCGAATGCACGCTTGCCAAACGGACGTCCATTTCCACCACTATTCAACTTCAAACACGAACTAGCAAATTCTTCTCAAGAGCTCATCAGCAGGCTCATACCAGAACATGTTCGACGGCAAGCTACCCACAACTTCTTCAATACTGGGAGCTAAAAATGCTAAATGCACACCACCAGACCTTTTGTTGGATCGTTTTCGCGGAACCGGTGAAGTTCACATGAAGGCTGAGTCAGATGATTCTTCGAATCCCCGCAAAACAAGAAGAATAGAAAATATGATTCCTCAGATGATGATATGCAAATGCTTCGTTGGAAGTTCAATTCAATCATCGAAGAAGAACAACATTGTAAATCGAGAAGTTTTTGCATCGCGAGTTTGGTAGTGAAACCGGGATCAGCTGGTATGACGGAGGAAACCGAAATGTTTAGATCCATGACTGAGTTTTCTTTCATTTTTTTTGCCCAATTGTAACAGAAGAATATAGTTCCCTAATGTAGGCGTAGTTGTAACCTGTAAACTGCCACTGTTTTGTTCACATTCCATGATGTAAATGCCACCATGCCTCTGATGAATAACTCTCCTTGTAACCTTGTTCCTTCCATCCTTGACTGTTTACCTTAAAGCCGTGGACAGTGTACACTGTACATGTACCGTGCTACACGGAAGGACATATTTGAATTTTTTTTCTCTC

CDS Sequence

>LOC_Os05g11730.1
ATGGACCAGCCGGCGCCGGCGCCGGAGCCGATGCTGCTCGACGCGCAGCCGCCCGCCGCCGTCGCCTGCGACAAGAAGCAGCAGGAGGGGGAGGCGCCGTACGCGGAGGGGAATGACGCGGTGACCGGGCACATCATCTCCACCACCATCGGGGGCAAGAACGGCGAGCCCAAGAGGACAATTAGCTACATGGCGGAGCGCGTTGTGGGCACTGGTTCTTTCGGTATCGTCTTTCAGGCTAAATGCTTGGAGACAGGAGAGACTGTTGCCATTAAGAAGGTATTGCAGGACCGACGGTACAAGAACCGTGAGCTTCAGCTTATGCGCGCCATGGACCACCCCAATGTCATCTCCCTGAAGCATTGCTTCTTCTCAACCACAAGTAGGGATGAGCTGTTCCTCAATCTTGTCATGGAATATGTTCCAGAAACACTCTACCGTGTGCTTAAGCACTACAGCAATGCCAACCACCGGATGCCACTTATCTACGTCAAGCTTTACATGTATCAGTTATTTAGGGGGCTTGCGTACATTCATACTGTTCCAGGGGTCTGTCATAGGGATGTGAAGCCACAAAATGTTTTGGTGGATCCTCTAACTCATCAAGTCAAGCTCTGTGACTTTGGGAGCGCAAAAACACTGGTCCCAGGTGAACCCAATATATCATATATATGCTCACGCTACTACCGAGCACCGGAGCTCATATTTGGTGCAACTGAATATACTACATCAATAGATATATGGTCAGCTGGGTGTGTTCTTGCAGAGCTACTCCTTGGTCAGCCATTGTTTCCAGGGGAGAGTGCAGTCGATCAGCTTGTAGAGATAATTAAGGTTCTTGGTACACCAACCCGTGAGGAAATACGTTGCATGAACCCGAACTATACAGAGTTTAGGTTTCCACAGATAAAAGCTCACCCTTGGCACAAGGTTTTCCACAAGAGGATGCCTCCTGAAGCAATAGACCTCGCTTCACGCCTTCTTCAATATTCACCGAGTCTCCGCTGCACTGCTCTTGATGCATGTGCACATCCTTTCTTTGATGAGCTGCGAGAGCCGAATGCACGCTTGCCAAACGGACGTCCATTTCCACCACTATTCAACTTCAAACACGAACTAGCAAATTCTTCTCAAGAGCTCATCAGCAGGCTCATACCAGAACATGTTCGACGGCAAGCTACCCACAACTTCTTCAATACTGGGAGCTAA

Protein Sequence

>LOC_Os05g11730.1
MDQPAPAPEPMLLDAQPPAAVACDKKQQEGEAPYAEGNDAVTGHIISTTIGGKNGEPKRTISYMAERVVGTGSFGIVFQAKCLETGETVAIKKVLQDRRYKNRELQLMRAMDHPNVISLKHCFFSTTSRDELFLNLVMEYVPETLYRVLKHYSNANHRMPLIYVKLYMYQLFRGLAYIHTVPGVCHRDVKPQNVLVDPLTHQVKLCDFGSAKTLVPGEPNISYICSRYYRAPELIFGATEYTTSIDIWSAGCVLAELLLGQPLFPGESAVDQLVEIIKVLGTPTREEIRCMNPNYTEFRFPQIKAHPWHKVFHKRMPPEAIDLASRLLQYSPSLRCTALDACAHPFFDELREPNARLPNGRPFPPLFNFKHELANSSQELISRLIPEHVRRQATHNFFNTGS*