Gene Details:

• MSU gene ID: LOC_Os05g10670
• RAPdb gene ID: Os05g0195101
• Gene Symbol: OsTZF1
• Genome: MSU7 ,  IRGSP-1.0
• Species: Oryza sativa

Functional Descriptions:

• The OsTZF1 gene was expressed at relatively high levels in leaves and shoots, although its transcripts were detected in various organs.
• Expression of OsTZF1 was induced by drought, high-salt stress, and hydrogen peroxide.
• Ubi:OsTZF1-OX plants showed improved tolerance to high-salt and drought stresses and vice versa for OsTZF1-RNAi plants.
• Histochemical activity of beta-glucuronidase in transgenic rice plants containing the promoter of OsTZF1 fused with beta-glucuronidase was observed in callus, coleoptile, young leaf, and panicle tissues.
• In addition, OsTZF1 expression was regulated by salt, PEG, and ABA.
• Microarray analysis revealed that genes related to stress, reactive oxygen species homeostasis, and metal homeostasis were regulated in the Ubi:OsTZF1-OX plants.
• Overexpression of OsTZF1 caused a long leaf sheath relative to wild type (WT) under R and FR, suggesting that OsTZF1 probably acts as a negative regulator of photomorphogenesis in rice seedlings.
• Transgenic rice plants overexpressing OsTZF1 driven by a maize (Zea mays) ubiquitin promoter (Ubi:OsTZF1-OX [for overexpression]) exhibited delayed seed germination, growth retardation at the seedling stage, and delayed leaf senescence.
• RNA interference (RNAi) knocked-down plants (OsTZF1-RNAi) showed early seed germination, enhanced seedling growth, and early leaf senescence compared with controls.
• Here, we investigated a cytoplasmic TZF protein, OsTZF1, which is involved in photomorphogenesis and ABA responses in rice seedlings.
• Moreover, ABA-induced growth inhibition of rice seedlings was marked in the OsTZF1-overexpression lines compared with WT, suggesting the positive regulation of OsTZF1 to ABA responses.
• Overexpression of a phytochrome-regulated tandem zinc finger protein gene, OsTZF1, confers hypersensitivity to ABA and hyposensitivity to red light and far-red light in rice seedlings.
• OsTZF1 gene expression was also induced by abscisic acid, methyl jasmonate, and salicylic acid.
• OsTZF1, a CCCH-tandem zinc finger protein, confers delayed senescence and stress tolerance in rice by regulating stress-related genes.
• KEY MESSAGE: OsTZF1 encodes a cytoplasm-localized tandem zinc finger protein and is regulated by both ABA and phytochrome-mediated light signaling.
• OsTZF1 functions in phytochrome-mediated light and ABA responses in rice.
• Here, we report that transgenic rice plants overexpressing OsTZF1 under own promoter (P(OsTZF1):OsTZF1-OX [for overexpression]) transferred to soil showed normal growth similar to vector control plants.

Function-related keywords:

• shoot ,  salt ,  panicle ,  homeostasis ,  leaf ,  sheath ,  growth ,  seedling ,  salicylic-acid ,  drought ,  senescence ,  seed ,  salt-stress ,  ABA ,  seed-germination ,  jasmonate

Literature:

• Overexpression of a phytochrome-regulated tandem zinc finger protein gene, OsTZF1, confers hypersensitivity to ABA and hyposensitivity to red light and far-red light in rice seedlings .  DOI:  10.1007/s00299-012-1252-x ;  PMID:  22572927
• OsTZF1, a CCCH-tandem zinc finger protein, confers delayed senescence and stress tolerance in rice by regulating stress-related genes .  DOI:  10.1104/pp.112.205385 ;  PMID:  23296688
• OsTZF1, a CCCH-tandem zinc finger protein gene, driven under own promoter produces no pleiotropic effects and confers salt and drought tolerance in rice .  DOI:  10.1080/15592324.2022.2142725 ;  PMID:  36398733

Related News:

Gene Resources:

• NCBI ID: AK106392
• UniProt accessions:

Sequences: