Gene Details:

Functional Descriptions:

  • Overexpression of OsWRKY67 in rice confirmed enhanced disease resistance, but led to a restriction of plant growth in transgenic lines with high levels of OsWRKY67 protein.
  • OsWRKY67 Plays a Positive Role in Basal and XA21-Mediated Resistance in Rice.
  • Activation of OsWRKY67 by T-DNA tagging significantly improved the resistance against two rice pathogens, Magnaporthe oryzae and Xanthomonas oryzae pv.
  • OsWRKY67 RNAi lines significantly reduced resistance to M.
  • oryzae and Xoo isolates tested, and abolished XA21-mediated resistance, implying the possibility of broad-spectrum resistance from OsWRKY67.
  • Therefore, these results suggest that OsWRKY67 positively regulates basal and XA21-mediated resistance, and is a promising candidate for genetic improvement of disease resistance in rice.
  • Transcriptional activity and subcellular localization assays indicated that OsWRKY67 is present in the nucleus where it functions as a transcriptional activator.
  • Reactive oxygen species (ROS) rapidly accumulated in OsWRKY67 activation mutant lines in response to elicitor treatment, compared with the controls.
  • Here, we identified that the transcription of OsWRKY67 was strongly activated by leaf and panicle blast infection.
  • Rice plants overexpressing OsWRKY67 showed quantitatively enhanced resistance to leaf blast, panicle blast and bacterial blight.
  • These results together suggest the positive role of OsWRKY67 in regulating rice responses to leaf blast, panicle blast and bacterial blight disease.
  • OsWRKY67 positively regulates blast and bacteria blight resistance by direct activation of PR genes in rice.
  • RNA-seq analysis indicated that OsWRKY67 induces the transcription of a set of defense-related genes including the ones involved in the salicylic acid (SA)-dependent pathway.
  • In contrast, silencing of OsWRKY67 increased the susceptibility to blast and bacterial blight diseases.
  • OsWRKY67 is ubiquitously expressed and sub-localized in the nucleus.

Literature:

Gene Resources:

  • NCBI ID:
  • UniProt accessions:

Sequences:

cDNA Sequence
  • >LOC_Os05g09020.1
    ATCCATTACCATCACAGAAGAAAATTCACCGGTGAAAAGGTTCAGCACTGTGCAATGGCGGCTTCCGTAGGACTGAACCCTGAAGCTTTCTTCTTCAGCAACTCCTACTCCTACTCCTCATCCCCTTTCATGGCCAGCTACACGCCGGAGTTCTCGGCCGCCGCTATCGACGCCAACTTATTCTCCGGCGAGCTCGATTTCGACTGCTCTCTCCCAGCTCCGGCCCAGGAGTACCCGGAAAATGAAAACACTATGATGAGGTACGAGAGCGAGGAGAAGATGAGGGCGAGGGTGAACGGGAGGATCGGGTTCAGGACGAGGTCGGAGGTGGAGATTCTTGATGATGGCTTCAAGTGGAGGAAGTACGGCAAGAAAGCTGTCAAGAACAGCCCAAATCCAAGAAACTACTACCGGTGCTCGACGGAGGGGTGCAACGTGAAGAAGCGAGTGGAGAGAGACCGGGAGGACCACCGCTACGTCATCACCACCTACGACGGCGTCCACAACCACGCGAGCCCCGCCGCCGCCGCCGCGGCGCTGCAGTACGCCGCCGCCGCCGGCGACTACTACAGCCCGCCGCTCAGCAGCGCCGGCTCGCCGCCGGCCGCCTATTCGGCAGGCGGCTCGCTGCTCTTCTGAGGCTGATGAGTTTGTCTAGTACTAGACACGCCCGGGAGCTCTAGCTAGGATAGAAAACTAGAGAAAGTTTAATTATACCTGAATTTTAATTAATCACCTCACAAAACTCTGATGTATCATATTATATCGTGATAGTGACTGCTTGTTAATTTAATTTTGCCGATCGATGTGATCTTCGGTTCGTGTGTTCATGACTTAACGTCTTCATGTATGTTTTTAGCAGAAAGATCAACTGCTGGTTTTAATATACA
  • >LOC_Os05g09020.2
    ATCCATTACCATCACAGAAGAAAATTCACCGGTGAAAAGGTTCAGCACTGTGCAATGGCGGCTTCCGTAGGACTGAACCCTGAAGCTTTCTTCTTCAGCAACTCCTACTCCTACTCCTCATCCCCTTTCATGGCCAGCTACACGCCGGAGTTCTCGGCCGCCGCTATCGACGCCAACTTATTCTCCGGCGAGCTCGATTTCGACTGCTCTCTCCCAGCTCCGGCCCAGGAGTACCCGGAAAATGAAAACACTATGATGAGGTACGAGAGCGAGGAGAAGATGAGGGCGAGGGTGAACGGGAGGATCGGGTTCAGGACGAGGTCGGAGGTGGAGATTCTTGATGATGGCTTCAAGTGGAGGAAGTACGGCAAGAAAGCTGTCAAGAACAGCCCAAATCCAAGAAACTACTACCGGTGCTCGACGGAGGGGTGCAACGTGAAGAAGCGAGTGGAGAGAGACCGGGAGGACCACCGCTACGTCATCACCACCTACGACGGCGTCCACAACCACGCGAGCCCCGCCGCCGCCGCCGCGGCGCTGCAGTACGCCGCCGCCGCCGGCGACTACTACAGCCCGCCGCTCAGCAGCGCCGGCTCGCCGCCGGCCGCCTATTCGGCAGGCGGCTCGCTGCTCTTCTGAGGCTGATGAGTTTGTCTAGTACTAGACACGCCCGGGAGCTCTAGCTAGGATAGAAAACTAGAGAAAGTTTAATTATACCTGAATTTTAATTAATCACCTCACAAAACTCTGATGTATCATATTATATCGTGATAGTGACTGCTTGTTAATTTAATTTTGCCGATCGATGTGATCTTCGGTTCGTGTGTTCATGACTTAACGTCTTCATGTATGTTTTTAGCAGAAAGATCAACTGCTGGTTTTAATATACA
CDS Sequence
  • >LOC_Os05g09020.1
    ATGGCGGCTTCCGTAGGACTGAACCCTGAAGCTTTCTTCTTCAGCAACTCCTACTCCTACTCCTCATCCCCTTTCATGGCCAGCTACACGCCGGAGTTCTCGGCCGCCGCTATCGACGCCAACTTATTCTCCGGCGAGCTCGATTTCGACTGCTCTCTCCCAGCTCCGGCCCAGGAGTACCCGGAAAATGAAAACACTATGATGAGGTACGAGAGCGAGGAGAAGATGAGGGCGAGGGTGAACGGGAGGATCGGGTTCAGGACGAGGTCGGAGGTGGAGATTCTTGATGATGGCTTCAAGTGGAGGAAGTACGGCAAGAAAGCTGTCAAGAACAGCCCAAATCCAAGAAACTACTACCGGTGCTCGACGGAGGGGTGCAACGTGAAGAAGCGAGTGGAGAGAGACCGGGAGGACCACCGCTACGTCATCACCACCTACGACGGCGTCCACAACCACGCGAGCCCCGCCGCCGCCGCCGCGGCGCTGCAGTACGCCGCCGCCGCCGGCGACTACTACAGCCCGCCGCTCAGCAGCGCCGGCTCGCCGCCGGCCGCCTATTCGGCAGGCGGCTCGCTGCTCTTCTGA
  • >LOC_Os05g09020.2
    ATGGCGGCTTCCGTAGGACTGAACCCTGAAGCTTTCTTCTTCAGCAACTCCTACTCCTACTCCTCATCCCCTTTCATGGCCAGCTACACGCCGGAGTTCTCGGCCGCCGCTATCGACGCCAACTTATTCTCCGGCGAGCTCGATTTCGACTGCTCTCTCCCAGCTCCGGCCCAGGAGTACCCGGAAAATGAAAACACTATGATGAGGTACGAGAGCGAGGAGAAGATGAGGGCGAGGGTGAACGGGAGGATCGGGTTCAGGACGAGGTCGGAGGTGGAGATTCTTGATGATGGCTTCAAGTGGAGGAAGTACGGCAAGAAAGCTGTCAAGAACAGCCCAAATCCAAGAAACTACTACCGGTGCTCGACGGAGGGGTGCAACGTGAAGAAGCGAGTGGAGAGAGACCGGGAGGACCACCGCTACGTCATCACCACCTACGACGGCGTCCACAACCACGCGAGCCCCGCCGCCGCCGCCGCGGCGCTGCAGTACGCCGCCGCCGCCGGCGACTACTACAGCCCGCCGCTCAGCAGCGCCGGCTCGCCGCCGGCCGCCTATTCGGCAGGCGGCTCGCTGCTCTTCTGA
Protein Sequence
  • >LOC_Os05g09020.1
    MAASVGLNPEAFFFSNSYSYSSSPFMASYTPEFSAAAIDANLFSGELDFDCSLPAPAQEYPENENTMMRYESEEKMRARVNGRIGFRTRSEVEILDDGFKWRKYGKKAVKNSPNPRNYYRCSTEGCNVKKRVERDREDHRYVITTYDGVHNHASPAAAAAALQYAAAAGDYYSPPLSSAGSPPAAYSAGGSLLF*
  • >LOC_Os05g09020.2
    MAASVGLNPEAFFFSNSYSYSSSPFMASYTPEFSAAAIDANLFSGELDFDCSLPAPAQEYPENENTMMRYESEEKMRARVNGRIGFRTRSEVEILDDGFKWRKYGKKAVKNSPNPRNYYRCSTEGCNVKKRVERDREDHRYVITTYDGVHNHASPAAAAAALQYAAAAGDYYSPPLSSAGSPPAAYSAGGSLLF*