Gene Details:

Functional Descriptions:

  • We have cloned two gibberellin (GA) 3 beta-hydroxylase genes, OsGA3ox1 and OsGA3ox2, from rice by screening a genomic library with a DNA fragment obtained by PCR using degenerate primers.
  • Although both genes show transient expression, the highest level for OsGA3ox1 is from unopened flower.
  • In addition, we generate an OsGA3ox1 knockout mutant by genome editing and demonstrate the bioactive gibberellic acid synthesis by the OsGA3ox1 action during starch accumulation in pollen via invertase regulation.

Literature:

Gene Resources:

Sequences:

cDNA Sequence
  • >LOC_Os05g08540.1
    GTACATCACTAGTCACAGGCTTTTGTGCGTTAGCCAACGAGTTCATGCAGATCATGACATCGTCGTCGACCTCGCCGACCTCGCCGACCTCGCCGCTGGCCGCCGCCGCCGACAATGGCGTCGCCGCCGCCTACTTCAACTTCCGCGGGGCGGAGCGCGTGCCGGAGTCGCACGTGTGGAAGGGGATGCACGAGAAGGACACCGCGCCGGTGGCGGCGGCGGACGCGGACGGCGGCGACGCGGTGCCGGTGGTGGACATGAGCGGCGGCGACGACGCCGCGGTGGCGGCGGTGGCGCGCGCGGCGGAGGAGTGGGGCGGGTTCCTGCTCGTCGGGCACGGCGTGACCGCGGAGGCCCTGGCGCGCGTCGAGGCGCAGGCGGCGCGGCTGTTCGCGCTGCCGGCGGACGACAAGGCGCGCGGGGCGCGGCGGCCCGGCGGCGGGAACACCGGCTACGGCGTGCCGCCGTACCTCCTCCGGTACCCGAAGCAGATGTGGGCCGAGGGCTACACCTTCCCTCCCCCTGCCATCCGCGACGAGTTCCGCCGCGTCTGGCCCGACGCCGGCGACGACTACCACCGCTTCTGCTCCGCCATGGAGGAGTACGACTCGTCGATGAGAGCTCTGGGCGAGAGGCTCCTCGCCATGTTCTTCAAGGCGCTCGGGCTCGCCGGCAACGATGCCCCCGGCGGCGAGACCGAGCGGAAGATCCGCGAAACGTTGACGTCGACGATTCACCTCAACATGTTCCCTAGGTGTCCAGATCCAGACCGGGTGGTCGGGCTGGCGGCGCACACGGACTCAGGCTTCTTCACCTTCATCCTGCAGAGCCCCGTGCCGGGGTTGCAGCTGCTCCGCCACCGGCCGGACCGGTGGGTGACGGTTCCGGGGACGCCGGGGGCGCTCATCGTCGTCGTCGGCGATCTCTTCCATGTGCTCACCAACGGGCGCTTCCACAGCGTGTTCCACCGCGCCGTCGTGAACCGGGAGAGAGACCGGATCTCCATGCCCTACTTCCTCGGTCCGCCGGCCGACATGAAGGTGACACCTCTCGTGGCGGCGGGGTCGCCGGAGAGCAAGGCCGTGTATCAGGCCGTGACATGGCCGGAGTACATGGCTGTAAGGGATAAGTTGTTCGGGACAAATATATCGGCGTTGAGCATGATTCGAGTAGCGAAGGAAGAGGACAAGGAGAGTTAGAACTATGGTATGATTGCAATTATCCATGCCAGAAAAAAAAAATTAGGGATATGATTTGTTGTGCTAAAATAAGGAAGGCAAATTTTAATTATAAGCAAGTATAGGTAGTTTTCCCTTGAATAAACTTTTTTTCTTAACAGCTTTCATAAATTGGAAAGGTGAATACTGACCTGATGAGAGTTTCACTGTAGAACCAGGACAAGGAATAAGGAATAAGTTGAGGCAACCTGAACAATGCT
CDS Sequence
  • >LOC_Os05g08540.1
    ATGCAGATCATGACATCGTCGTCGACCTCGCCGACCTCGCCGACCTCGCCGCTGGCCGCCGCCGCCGACAATGGCGTCGCCGCCGCCTACTTCAACTTCCGCGGGGCGGAGCGCGTGCCGGAGTCGCACGTGTGGAAGGGGATGCACGAGAAGGACACCGCGCCGGTGGCGGCGGCGGACGCGGACGGCGGCGACGCGGTGCCGGTGGTGGACATGAGCGGCGGCGACGACGCCGCGGTGGCGGCGGTGGCGCGCGCGGCGGAGGAGTGGGGCGGGTTCCTGCTCGTCGGGCACGGCGTGACCGCGGAGGCCCTGGCGCGCGTCGAGGCGCAGGCGGCGCGGCTGTTCGCGCTGCCGGCGGACGACAAGGCGCGCGGGGCGCGGCGGCCCGGCGGCGGGAACACCGGCTACGGCGTGCCGCCGTACCTCCTCCGGTACCCGAAGCAGATGTGGGCCGAGGGCTACACCTTCCCTCCCCCTGCCATCCGCGACGAGTTCCGCCGCGTCTGGCCCGACGCCGGCGACGACTACCACCGCTTCTGCTCCGCCATGGAGGAGTACGACTCGTCGATGAGAGCTCTGGGCGAGAGGCTCCTCGCCATGTTCTTCAAGGCGCTCGGGCTCGCCGGCAACGATGCCCCCGGCGGCGAGACCGAGCGGAAGATCCGCGAAACGTTGACGTCGACGATTCACCTCAACATGTTCCCTAGGTGTCCAGATCCAGACCGGGTGGTCGGGCTGGCGGCGCACACGGACTCAGGCTTCTTCACCTTCATCCTGCAGAGCCCCGTGCCGGGGTTGCAGCTGCTCCGCCACCGGCCGGACCGGTGGGTGACGGTTCCGGGGACGCCGGGGGCGCTCATCGTCGTCGTCGGCGATCTCTTCCATGTGCTCACCAACGGGCGCTTCCACAGCGTGTTCCACCGCGCCGTCGTGAACCGGGAGAGAGACCGGATCTCCATGCCCTACTTCCTCGGTCCGCCGGCCGACATGAAGGTGACACCTCTCGTGGCGGCGGGGTCGCCGGAGAGCAAGGCCGTGTATCAGGCCGTGACATGGCCGGAGTACATGGCTGTAAGGGATAAGTTGTTCGGGACAAATATATCGGCGTTGAGCATGATTCGAGTAGCGAAGGAAGAGGACAAGGAGAGTTAG
Protein Sequence
  • >LOC_Os05g08540.1
    MQIMTSSSTSPTSPTSPLAAAADNGVAAAYFNFRGAERVPESHVWKGMHEKDTAPVAAADADGGDAVPVVDMSGGDDAAVAAVARAAEEWGGFLLVGHGVTAEALARVEAQAARLFALPADDKARGARRPGGGNTGYGVPPYLLRYPKQMWAEGYTFPPPAIRDEFRRVWPDAGDDYHRFCSAMEEYDSSMRALGERLLAMFFKALGLAGNDAPGGETERKIRETLTSTIHLNMFPRCPDPDRVVGLAAHTDSGFFTFILQSPVPGLQLLRHRPDRWVTVPGTPGALIVVVGDLFHVLTNGRFHSVFHRAVVNRERDRISMPYFLGPPADMKVTPLVAAGSPESKAVYQAVTWPEYMAVRDKLFGTNISALSMIRVAKEEDKES*