Gene Details:
- MSU gene ID: LOC_Os05g08540
- RAPdb gene ID: Os05g0178100
- Gene Symbol: OsGA3ox1
- Genome: MSU7 , IRGSP-1.0
- Species: Oryza sativa
Functional Descriptions:
- We have cloned two gibberellin (GA) 3 beta-hydroxylase genes, OsGA3ox1 and OsGA3ox2, from rice by screening a genomic library with a DNA fragment obtained by PCR using degenerate primers.
- Although both genes show transient expression, the highest level for OsGA3ox1 is from unopened flower.
- In addition, we generate an OsGA3ox1 knockout mutant by genome editing and demonstrate the bioactive gibberellic acid synthesis by the OsGA3ox1 action during starch accumulation in pollen via invertase regulation.
Function-related keywords:
- gibberellin , flower , pollen , starch , starch-accumulation
Literature:
- Gibberellin homeostasis and plant height control by EUI and a role for gibberellin in root gravity responses in rice . DOI: 10.1038/cr.2008.28 ; PMID: 18268540
- Cloning and functional analysis of two gibberellin 3 beta -hydroxylase genes that are differently expressed during the growth of rice . DOI: 10.1073/pnas.141239398 ; PMID: 11438692
- Evolutionary alterations in gene expression and enzymatic activities of gibberellin 3-oxidase 1 in Oryza . DOI: 10.1038/s42003-022-03008-5 ; PMID: 35046494
Related News:
Gene Resources:
- NCBI ID: AB054084
- UniProt accessions:
Sequences:
cDNA Sequence
- >LOC_Os05g08540.1
GTACATCACTAGTCACAGGCTTTTGTGCGTTAGCCAACGAGTTCATGCAGATCATGACATCGTCGTCGACCTCGCCGACCTCGCCGACCTCGCCGCTGGCCGCCGCCGCCGACAATGGCGTCGCCGCCGCCTACTTCAACTTCCGCGGGGCGGAGCGCGTGCCGGAGTCGCACGTGTGGAAGGGGATGCACGAGAAGGACACCGCGCCGGTGGCGGCGGCGGACGCGGACGGCGGCGACGCGGTGCCGGTGGTGGACATGAGCGGCGGCGACGACGCCGCGGTGGCGGCGGTGGCGCGCGCGGCGGAGGAGTGGGGCGGGTTCCTGCTCGTCGGGCACGGCGTGACCGCGGAGGCCCTGGCGCGCGTCGAGGCGCAGGCGGCGCGGCTGTTCGCGCTGCCGGCGGACGACAAGGCGCGCGGGGCGCGGCGGCCCGGCGGCGGGAACACCGGCTACGGCGTGCCGCCGTACCTCCTCCGGTACCCGAAGCAGATGTGGGCCGAGGGCTACACCTTCCCTCCCCCTGCCATCCGCGACGAGTTCCGCCGCGTCTGGCCCGACGCCGGCGACGACTACCACCGCTTCTGCTCCGCCATGGAGGAGTACGACTCGTCGATGAGAGCTCTGGGCGAGAGGCTCCTCGCCATGTTCTTCAAGGCGCTCGGGCTCGCCGGCAACGATGCCCCCGGCGGCGAGACCGAGCGGAAGATCCGCGAAACGTTGACGTCGACGATTCACCTCAACATGTTCCCTAGGTGTCCAGATCCAGACCGGGTGGTCGGGCTGGCGGCGCACACGGACTCAGGCTTCTTCACCTTCATCCTGCAGAGCCCCGTGCCGGGGTTGCAGCTGCTCCGCCACCGGCCGGACCGGTGGGTGACGGTTCCGGGGACGCCGGGGGCGCTCATCGTCGTCGTCGGCGATCTCTTCCATGTGCTCACCAACGGGCGCTTCCACAGCGTGTTCCACCGCGCCGTCGTGAACCGGGAGAGAGACCGGATCTCCATGCCCTACTTCCTCGGTCCGCCGGCCGACATGAAGGTGACACCTCTCGTGGCGGCGGGGTCGCCGGAGAGCAAGGCCGTGTATCAGGCCGTGACATGGCCGGAGTACATGGCTGTAAGGGATAAGTTGTTCGGGACAAATATATCGGCGTTGAGCATGATTCGAGTAGCGAAGGAAGAGGACAAGGAGAGTTAGAACTATGGTATGATTGCAATTATCCATGCCAGAAAAAAAAAATTAGGGATATGATTTGTTGTGCTAAAATAAGGAAGGCAAATTTTAATTATAAGCAAGTATAGGTAGTTTTCCCTTGAATAAACTTTTTTTCTTAACAGCTTTCATAAATTGGAAAGGTGAATACTGACCTGATGAGAGTTTCACTGTAGAACCAGGACAAGGAATAAGGAATAAGTTGAGGCAACCTGAACAATGCT
CDS Sequence
- >LOC_Os05g08540.1
ATGCAGATCATGACATCGTCGTCGACCTCGCCGACCTCGCCGACCTCGCCGCTGGCCGCCGCCGCCGACAATGGCGTCGCCGCCGCCTACTTCAACTTCCGCGGGGCGGAGCGCGTGCCGGAGTCGCACGTGTGGAAGGGGATGCACGAGAAGGACACCGCGCCGGTGGCGGCGGCGGACGCGGACGGCGGCGACGCGGTGCCGGTGGTGGACATGAGCGGCGGCGACGACGCCGCGGTGGCGGCGGTGGCGCGCGCGGCGGAGGAGTGGGGCGGGTTCCTGCTCGTCGGGCACGGCGTGACCGCGGAGGCCCTGGCGCGCGTCGAGGCGCAGGCGGCGCGGCTGTTCGCGCTGCCGGCGGACGACAAGGCGCGCGGGGCGCGGCGGCCCGGCGGCGGGAACACCGGCTACGGCGTGCCGCCGTACCTCCTCCGGTACCCGAAGCAGATGTGGGCCGAGGGCTACACCTTCCCTCCCCCTGCCATCCGCGACGAGTTCCGCCGCGTCTGGCCCGACGCCGGCGACGACTACCACCGCTTCTGCTCCGCCATGGAGGAGTACGACTCGTCGATGAGAGCTCTGGGCGAGAGGCTCCTCGCCATGTTCTTCAAGGCGCTCGGGCTCGCCGGCAACGATGCCCCCGGCGGCGAGACCGAGCGGAAGATCCGCGAAACGTTGACGTCGACGATTCACCTCAACATGTTCCCTAGGTGTCCAGATCCAGACCGGGTGGTCGGGCTGGCGGCGCACACGGACTCAGGCTTCTTCACCTTCATCCTGCAGAGCCCCGTGCCGGGGTTGCAGCTGCTCCGCCACCGGCCGGACCGGTGGGTGACGGTTCCGGGGACGCCGGGGGCGCTCATCGTCGTCGTCGGCGATCTCTTCCATGTGCTCACCAACGGGCGCTTCCACAGCGTGTTCCACCGCGCCGTCGTGAACCGGGAGAGAGACCGGATCTCCATGCCCTACTTCCTCGGTCCGCCGGCCGACATGAAGGTGACACCTCTCGTGGCGGCGGGGTCGCCGGAGAGCAAGGCCGTGTATCAGGCCGTGACATGGCCGGAGTACATGGCTGTAAGGGATAAGTTGTTCGGGACAAATATATCGGCGTTGAGCATGATTCGAGTAGCGAAGGAAGAGGACAAGGAGAGTTAG
Protein Sequence
- >LOC_Os05g08540.1
MQIMTSSSTSPTSPTSPLAAAADNGVAAAYFNFRGAERVPESHVWKGMHEKDTAPVAAADADGGDAVPVVDMSGGDDAAVAAVARAAEEWGGFLLVGHGVTAEALARVEAQAARLFALPADDKARGARRPGGGNTGYGVPPYLLRYPKQMWAEGYTFPPPAIRDEFRRVWPDAGDDYHRFCSAMEEYDSSMRALGERLLAMFFKALGLAGNDAPGGETERKIRETLTSTIHLNMFPRCPDPDRVVGLAAHTDSGFFTFILQSPVPGLQLLRHRPDRWVTVPGTPGALIVVVGDLFHVLTNGRFHSVFHRAVVNRERDRISMPYFLGPPADMKVTPLVAAGSPESKAVYQAVTWPEYMAVRDKLFGTNISALSMIRVAKEEDKES*