Gene Details:

• MSU gene ID: LOC_Os05g06670
• RAPdb gene ID: Os05g0158600
• Gene Symbol: OsGA2ox1
• Genome: MSU7 ,  IRGSP-1.0
• Species: Oryza sativa

Functional Descriptions:

• Unlike the over-expression of OsGA2ox1, which led to a high rate of seed abortion, the H032 mutant retained normal flowering and seed production.
• Ectopic expression of theOsGA2ox1 cDNA in transgenic rice inhibited stem elongation and the development of reproductive organs.
• OsGA2ox1was expressed in shoot apex and roots but not in leaves and stems.
• These observations suggest that OsGA2ox1 controls the level of bioactive GAs in the shoot apical meristem; therefore, reduction in its expression may contribute to the early development of the inflorescence meristem.
• We have isolated and characterized a cDNA, designated Oryza sativa GA 2-oxidase 1 (OsGA2ox1) from rice (Oryza sativa L.
• These results indicate that OsGA2ox1 encodes a GA 2-oxidase, which is functional not only in vitro but also in vivo.
• In situ hybridization analysis revealed that OsGA2ox1 mRNA was localized in a ring at the basal region of leaf primordia and young leaves.
• When rice seedlings were subjected to chilling stress, the repressive effect of OsGRF6 on OsGA2ox1 was released by cold-induced SLR1, which activated OsGA2ox1 expression to decrease the active GA levels, enhancing chilling tolerance.
• OsGRF6 interacts with SLR1 to regulate OsGA2ox1 expression for coordinating chilling tolerance and growth in rice.
• The yeast one-hybrid, qRT-PCR, and transactivation assays showed that both SLR1 and OsGRF6 can bind to the promoter of the active GA catabolic gene OsGA2ox1, where SLR1 promoted and OsGRF6 suppressed OsGA2ox1 expression.
• At normal temperature, OsGRF6 was responsible for maintaining active GA levels by inhibiting OsGA2ox1.

Function-related keywords:

• flower ,  stem ,  meristem ,  ga ,  reproductive ,  shoot-apical-meristem ,  leaf ,  shoot ,  root ,  seed ,  inflorescence ,  seedlings ,  growth ,  tolerance ,  chilling ,  chilling-stress ,  GA

Literature:

• Activation of gibberellin 2-oxidase 6 decreases active gibberellin levels and creates a dominant semi-dwarf phenotype in rice (Oryza sativa L.) .  DOI:  10.1016/S1673-8527(09)60022-9 ;  PMID:  20171575
• Gibberellin homeostasis and plant height control by EUI and a role for gibberellin in root gravity responses in rice .  DOI:  10.1038/cr.2008.28 ;  PMID:  18268540
• OsDOG, a gibberellin-induced A20/AN1 zinc-finger protein, negatively regulates gibberellin-mediated cell elongation in rice .  DOI:  10.1016/j.jplph.2010.12.013 ;  PMID:  21316795
• Expression of a gibberellin 2-oxidase gene around the shoot apex is related to phase transition in rice .  DOI:  10.1104/pp.125.3.1508 ;  PMID:  11244129
• OsGRF6 interacts with SLR1 to regulate OsGA2ox1 expression for coordinating chilling tolerance and growth in rice .  DOI:  10.1016/j.jplph.2021.153406 ;  PMID:  33756268
• Orchestration of ethylene and gibberellin signals determines primary root elongation in rice .  DOI:  10.1093/plcell/koac008 ;  PMID:  35021223

Related News:

Gene Resources:

• NCBI ID:
• UniProt accessions:

Sequences: