Gene Details:

• MSU gene ID: LOC_Os05g05800
• RAPdb gene ID: Os05g0150500
• Gene Symbol: OsTIR1
• Genome: MSU7 ,  IRGSP-1.0
• Species: Oryza sativa

Functional Descriptions:

• Moreover, yeast two-hybrid and bimolecular fluorescence complementation assays showed that OsTIR1 and OsAFB2 interact with OsIAA1.
• OsTIR1 and OsAFB2 downregulation via OsmiR393 overexpression leads to more tillers, early flowering and less tolerance to salt and drought in rice.
• In this study, we verified that two rice auxin receptor gene homologs (OsTIR1 and OsAFB2) could be targeted by OsmiR393 (Os for Oryza sativa).
• These data indicated that the phenotypes of OsmiR393-overexpressing rice may be caused through hyposensitivity to the auxin signal by reduced expression of two auxin receptor genes (OsTIR1 and OsAFB2).
• The expression of an auxin transporter (OsAUX1) and a tillering inhibitor (OsTB1) were downregulated by overexpression of OsmiR393, which suggested that a gene chain from OsmiR393 to rice tillering may be from OsTIR1 and OsAFB2 to OsAUX1, which affected the transportation of auxin, then to OsTB1, which finally controlled tillering.
• Transcript analysis revealed that Pi-responsive genes–Phosphate starvation (OsIPS)1 and OsIPS2, SYG1/Pho81/XPR1(OsSPX1), Sulfoquinovosyldiacylglycerol 2 (OsSQD2), R2R3 MYB transcription factor (OsMYB2P-1) and Transport Inhibitor Response1 (OsTIR1)–were more abundant in the osarf12 and osarf12/25 mutants under +Pi/-Pi conditions.
• • This study functionally characterizes two members of the rice (Oryza sativa) miR393 family and their target genes, OsTIR1 and OsAFB2 (AUXIN SIGNALING F-BOX), the two closest homologs of Arabidopsis TRANSPORT INHIBITOR RESPONSE 1 (TIR1).
• We created targeted OsTIR1 and OsAFB2-5 mutations in rice using CRISPR/Cas9 genome editing, and functionally characterized the roles of these five members in plant growth and development and auxinic herbicide resistance.
• Together with our previous discovery that the viral P2 protein stabilizes OsIAA10 protein via thwarting its interaction with OsTIR1 to enhance viral infection and pathogenesis, our results reveal a novel auxin-IAA10-ARFs-mediated signaling mechanism employed by rice and RDV for defense and counter defense responses.

Function-related keywords:

• iaa ,  flower ,  auxin ,  drought ,  transporter ,  transcription-factor ,  phosphate ,  tiller ,  salt ,  tillering ,  growth ,  resistance ,  development ,  plant-growth ,  herbicide-resistance ,  defense ,  defense-response

Literature:

• OsTIR1 and OsAFB2 downregulation via OsmiR393 overexpression leads to more tillers, early flowering and less tolerance to salt and drought in rice .  DOI:  10.1371/journal.pone.0030039 ;  PMID:  22253868
• Auxin response factor (OsARF12), a novel regulator for phosphate homeostasis in rice (Oryza sativa) .  DOI:  10.1111/nph.12499 ;  PMID:  24111723
• Distinctive expression patterns and roles of the miRNA393/TIR1 homolog module in regulating flag leaf inclination and primary and crown root growth in rice (Oryza sativa) .  DOI:  10.1111/j.1469-8137.2012.04248.x ;  PMID:  22846038
• Functional analysis of auxin receptor OsTIR1/OsAFB family members in rice grain yield, tillering, plant height, root system, germination, and auxinic herbicide resistance .  DOI:  10.1111/nph.17061 ;  PMID:  33135782
• Auxin response factors (ARFs) differentially regulate rice antiviral immune response against rice dwarf virus .  DOI:  10.1371/journal.ppat.1009118 ;  PMID:  33264360

Related News:

Gene Resources:

• NCBI ID:
• UniProt accessions:

Sequences: