Information report for OsWRKY5
Gene Details
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Functional Descriptions
- OsWRKY5 Promotes Rice Leaf Senescence via Senescence-Associated NAC and Abscisic Acid Biosynthesis Pathway.
- Reverse-transcription quantitative PCR (RT-qPCR) showed that OsWRKY5 upregulates the expression of genes controlling chlorophyll degradation and leaf senescence.
- Taken together, OsWRKY5 is a positive regulator of leaf senescence that upregulates senescence-induced NAC, ABA biosynthesis, and chlorophyll degradation genes.
- Furthermore, OsWRKY5 expression was suppressed by ABA treatment.
- Inactivating transcription factor OsWRKY5 enhances drought tolerance through abscisic acid signaling pathways.
- Here, we show that the rice (Oryza sativa) WRKY transcription factor OsWRKY5 negatively regulates drought tolerance.
- We determined that OsWRKY5 was mainly expressed in developing leaves at the seedling and heading stages, and that its expression was reduced by drought stress and by treatment with NaCl, mannitol, and abscisic acid (ABA).
- Our results demonstrate that OsWRKY5 functions as a negative regulator of ABA-induced drought stress tolerance, strongly suggesting that inactivation of OsWRKY5 or manipulation of key OsWRKY5 targets could be useful to improve drought tolerance in rice cultivars.
- Transcriptome deep sequencing and RT-qPCR analyses demonstrated that the expression of abiotic stress-related genes including rice MYB2 (OsMYB2) was upregulated in OsWRKY5 knockout mutants and downregulated in OsWRKY5-D mutants.
- Moreover, dual-luciferase, yeast one-hybrid, and chromatin immunoprecipitation assays showed that OsWRKY5 directly binds to the W-box sequences in the promoter region of OsMYB2 and represses OsMYB2 expression, thus downregulating genes downstream of OsMYB2 in the ABA signaling pathways.
- Loss of OsWRKY5 activity increased sensitivity to ABA, thus promoting ABA-dependent stomatal closure.
Functional Keywords
Literature and News
- OsWRKY5 Promotes Rice Leaf Senescence via Senescence-Associated NAC and Abscisic Acid Biosynthesis Pathway . DOI: 10.3390/ijms20184437 ; PMID: 31505875
- Inactivating transcription factor OsWRKY5 enhances drought tolerance through abscisic acid signaling pathways . DOI: 10.1093/plphys/kiab492 ; PMID: 34718775
Gene Resources
- UniProt: Q75L34
- EMBL: AC093490, AP014961, BK005008
- AlphaFoldDB: Q75L34
- KEGG: osa:107276141
- InterPro: IPR003657, IPR036576, IPR044810
- PANTHER: PTHR31429, PTHR31429:SF41
- SUPFAM: SSF118290
- PROSITE: PS50811
- Gene3D: 2.20.25.80
- OrthoDB: Q75L34
- SWISS-MODEL: Q75L34
- eggNOG: ENOG502SM8W
Sequences
cDNA Sequence
- >LOC_Os05g04640.1
CTCTCCTTCTCACTCTACTGATCGATCGATCGAGCTCTATCCATGGAGATGATGGTGCAGAAGCAACGACACGAGGAGGGGGAAGAGGAGCGAGGAGGCTTGTGCGCCCGCGAGATCAAGGAGCTCGACTTCTTCTCCGCCGCCGGTGCCGGTGCCGGCCGGAGAGACGACGACGACGTCCTCCGTGCGGATGGGATCAGCAGCAGCCACGCCGGATTTATGGTCAGCACTGCGCTGGACTTGCTGACAGCGGTCAACGACGGTGATCATCATGAGGAGAAGAAAGGGCAGTCAAATATTCACCAAAGCAAGCAGATGGATGCGGCGGCGACGACGGTGGAGGGGGAGCTCCGGCAAGCCGGCGAAGAGAACCGGCGGCTGCGGCGGAGGCTGGAGGAGCTCACCAGCAGCTATGGCGCCCTCTACCACCAGCTCGTCCAGGCGCAGCAGCTGCACACCAAGCATCAGCAGCAGGCTCCGATCGCCGGCGTGCAGTTGCTGGACGCGCTCGCGGCGGCGTCTCCGGCGAGCCACCGGCGACGAGCGGCGGCGGCGGTGGACGGCGATAGAACAGCTGACTCTGATGGTGGCGAGGGCGACGAGAACGTGTCGCCTTCTCTTGGTAGCAAGAGGCCGGCGGCGGCAGCGACGTTAACGCGGCTGACGCCGGAGAGCGGCAGCGGCGGGGAGAATAATGGCGGCGGCGAGCAGGCGCCGGCGGCGGAGATGGCGCCGTGCCGGAAGGCCAGGGTGTCGGTGCGAGCACGATCCGAGGCTCCAATGATCAGTGATGGATGCCAATGGAGGAAGTACGGGCAGAAGATGGCCAAGGGTAACCCTTGCCCTAGAGCCTACTACCGATGCACAATGGCTTCGCAATGCCCCGTCAGAAAACAGGTGCAACGATGCGCGGAGGACAAGAGCATCCTCATCACCACCTACGAGGGCACCCACAGCCACCCGCTGCCGCCGGCCGCCGCCGCCATGGCCAAGACCACCTCCGCCGCCGCCGCCATGCTCCTCTCCGGCCCCGCCGTCAGCCGCGACGCGCTGTTCGCCGCCCACCACCACGTCGTCGCGCCGCCGCCCTTCTTCCACCACCCCTACGCCGGGTCCACCATGGCCACGCTCTCCGCCTCCGCCCCGTTCCCGACCATCACGCTCGACCTCACCCAGCCGCCGCCGACGACGACGACCACCGCCGCCGCCGCCATGCTCCAGCTCCACCGCCCTTATGCCTTCTCCTCATTGCCGTTCTCGATGTACGGCGCCGGCGGCGGCTCGCACCGGCCGCCCGTCGTCCTGCCCCCGCCGTCGTCGGTGGTGGAGACGATGACCGCCGCGATCACCAGGGACCCCAACTTCACCACGGCGGTGGCGGCCGCGCTCTCCTCGATCATGGCGGGAGGCGGCGCCCAAGCTCGAACACCTCCACGCGGCGGGAGTGACGCCGCCGGAGACATCAACGGAGGCGGCGGCGCCGACCACGCCACTGCCGGAGCACGCGCCGCGGCGGCGGCGACGCAGCCTTGCGGCACGTCTCCCACCTGA
CDS Sequence
- >LOC_Os05g04640.1
ATGGAGATGATGGTGCAGAAGCAACGACACGAGGAGGGGGAAGAGGAGCGAGGAGGCTTGTGCGCCCGCGAGATCAAGGAGCTCGACTTCTTCTCCGCCGCCGGTGCCGGTGCCGGCCGGAGAGACGACGACGACGTCCTCCGTGCGGATGGGATCAGCAGCAGCCACGCCGGATTTATGGTCAGCACTGCGCTGGACTTGCTGACAGCGGTCAACGACGGTGATCATCATGAGGAGAAGAAAGGGCAGTCAAATATTCACCAAAGCAAGCAGATGGATGCGGCGGCGACGACGGTGGAGGGGGAGCTCCGGCAAGCCGGCGAAGAGAACCGGCGGCTGCGGCGGAGGCTGGAGGAGCTCACCAGCAGCTATGGCGCCCTCTACCACCAGCTCGTCCAGGCGCAGCAGCTGCACACCAAGCATCAGCAGCAGGCTCCGATCGCCGGCGTGCAGTTGCTGGACGCGCTCGCGGCGGCGTCTCCGGCGAGCCACCGGCGACGAGCGGCGGCGGCGGTGGACGGCGATAGAACAGCTGACTCTGATGGTGGCGAGGGCGACGAGAACGTGTCGCCTTCTCTTGGTAGCAAGAGGCCGGCGGCGGCAGCGACGTTAACGCGGCTGACGCCGGAGAGCGGCAGCGGCGGGGAGAATAATGGCGGCGGCGAGCAGGCGCCGGCGGCGGAGATGGCGCCGTGCCGGAAGGCCAGGGTGTCGGTGCGAGCACGATCCGAGGCTCCAATGATCAGTGATGGATGCCAATGGAGGAAGTACGGGCAGAAGATGGCCAAGGGTAACCCTTGCCCTAGAGCCTACTACCGATGCACAATGGCTTCGCAATGCCCCGTCAGAAAACAGGTGCAACGATGCGCGGAGGACAAGAGCATCCTCATCACCACCTACGAGGGCACCCACAGCCACCCGCTGCCGCCGGCCGCCGCCGCCATGGCCAAGACCACCTCCGCCGCCGCCGCCATGCTCCTCTCCGGCCCCGCCGTCAGCCGCGACGCGCTGTTCGCCGCCCACCACCACGTCGTCGCGCCGCCGCCCTTCTTCCACCACCCCTACGCCGGGTCCACCATGGCCACGCTCTCCGCCTCCGCCCCGTTCCCGACCATCACGCTCGACCTCACCCAGCCGCCGCCGACGACGACGACCACCGCCGCCGCCGCCATGCTCCAGCTCCACCGCCCTTATGCCTTCTCCTCATTGCCGTTCTCGATGTACGGCGCCGGCGGCGGCTCGCACCGGCCGCCCGTCGTCCTGCCCCCGCCGTCGTCGGTGGTGGAGACGATGACCGCCGCGATCACCAGGGACCCCAACTTCACCACGGCGGTGGCGGCCGCGCTCTCCTCGATCATGGCGGGAGGCGGCGCCCAAGCTCGAACACCTCCACGCGGCGGGAGTGACGCCGCCGGAGACATCAACGGAGGCGGCGGCGCCGACCACGCCACTGCCGGAGCACGCGCCGCGGCGGCGGCGACGCAGCCTTGCGGCACGTCTCCCACCTGA
Protein Sequence
- >LOC_Os05g04640.1
MEMMVQKQRHEEGEEERGGLCAREIKELDFFSAAGAGAGRRDDDDVLRADGISSSHAGFMVSTALDLLTAVNDGDHHEEKKGQSNIHQSKQMDAAATTVEGELRQAGEENRRLRRRLEELTSSYGALYHQLVQAQQLHTKHQQQAPIAGVQLLDALAAASPASHRRRAAAAVDGDRTADSDGGEGDENVSPSLGSKRPAAAATLTRLTPESGSGGENNGGGEQAPAAEMAPCRKARVSVRARSEAPMISDGCQWRKYGQKMAKGNPCPRAYYRCTMASQCPVRKQVQRCAEDKSILITTYEGTHSHPLPPAAAAMAKTTSAAAAMLLSGPAVSRDALFAAHHHVVAPPPFFHHPYAGSTMATLSASAPFPTITLDLTQPPPTTTTTAAAAMLQLHRPYAFSSLPFSMYGAGGGSHRPPVVLPPPSSVVETMTAAITRDPNFTTAVAAALSSIMAGGGAQARTPPRGGSDAAGDINGGGGADHATAGARAAAAATQPCGTSPT*