Gene Details:
- MSU gene ID: LOC_Os05g04550
- RAPdb gene ID: Os05g0136200
- Gene Symbol: OsCIPK17
- Genome: MSU7 , IRGSP-1.0
- Species: Oryza sativa
Functional Descriptions:
- Functional Analysis of OsCIPK17 in Rice Grain Filling.
- The results showed that knockout of OsCIPK17 affected photosynthesis and starch-, sucrose-, and amino acid metabolism-related gene expression; furthermore, the mutation reduced PSII utilization efficiency, it blocked the synthesis and metabolism of starch and sucrose, and affected the formation and transport of assimilates, thereby reducing final grain weight.
- The results showed that OsCIPK17 improved drought resistance of rice by regulating deep roots under drought stress; Response to drought by regulating the energy metabolism pathway and controlling the accumulation of citric acid in the tricarboxylic acid (TCA) cycle; Our exogenous experiments also proved that OsCIPK17 responds to citric acid, and this process involves the auxin metabolism pathway; Exogenous citric acid can improve the drought resistance of overexpression plants.
- However, there is no report on the function and mechanism of OsCIPK17 in rice drought resistance.
- Our research reveals that OsCIPK17 positively regulates rice drought resistance and participates in the accumulation of citric acid in the TCA cycle, providing new insights for rice drought resistance.
- Combined metabolomic and transcriptomic analysis reveals key components of OsCIPK17 overexpression improves drought tolerance in rice.
- We combined transcriptional and metabonomic analysis to clarify the specific mechanism of OsCIPK17 in response to rice drought tolerance.
Function-related keywords:
- grain , starch , photosynthesis , grain-filling , grain-weight , auxin , resistance , drought , drought-stress , tolerance , drought-tolerance , drought-resistance , energy-metabolism
Literature:
- Functional Analysis of OsCIPK17 in Rice Grain Filling . DOI: 10.3389/fpls.2021.808312 ; PMID: 35145535
- Combined metabolomic and transcriptomic analysis reveals key components of OsCIPK17 overexpression improves drought tolerance in rice . DOI: 10.3389/fpls.2022.1043757 ; PMID: 36699859
Related News:
Gene Resources:
Sequences:
cDNA Sequence
- >LOC_Os05g04550.1
GTGCTGTAAACCTTGCTGCCCACTTGTCATCTCGTACTACTCCAGACACCTTCTTCCCCCTTCAAATTCAATCCTAAACTCATCAGGGAGTAGAGATCACTGTCAGAGATGCAAAAGCATCGAAGATAGTAGAGTCTTAGAGACAACAAGTGGGATCTTTATCAATAAATACCCTCCATTTTTTCTTGCCAATCCGTCTTCCATTATATTTCTCTCCCCTTCCAACAAATCGTCTCGAGAAAGAGGAGGAAGAATCGATCTTTTGGTGGAGTTTGCAATGGTGAAGGGAGGGAGGGAGGCGCTGCTGGGCGGGTACGAGATGGGGAGGACGCTCGGGGAGGGCAACTTCGGCAAGGTGAAGTACGCGCGCCACCTCGCCACCGGCGGCCACTTCGCCGTCAAGATCCTCGACCGCGGCCGCGTCGTCTCCCTCCGCGCCGGCGACCAGATTCGCCGCGAGATCGCCACGCTCAAGCTGCTCAGGCACCCCCACGTCGTCCGCCTCCACGAGGTTGCTGCTAGCAAAACTAAGATTTACATGGTGCTTGAGTTTGTCAATGGAGGTGAACTGTTTGAGAGAATTGCAGTAAAGGGAAAACTATCAGAGAAAGAAGGAAGGAGGCTTTTTCAGCAGTTAATTGATGGTGTTAGCTATTGCCATGATAGGGGAGTCTACCACAGAGACCTCAAGCCTGAGAACGTTCTTGTGGACCAAAAAGGCAACATCAAGATCTCTGATTTTGGTCTTAGTGCTTTACCTCAACACCTCGGGAATGATGGATTGCTGCATACTACCTGTGGTAGCCCCAACTATATTGCTCCTGAGGTTCTGCAGAATAAAGGATATGATGGATCCTTGTCAGATATCTGGTCTTGCGGAGTAATCCTTTATGTAATGCTTATAGGATATCTTCCGTTCGATGACCGAAATATTGTTGTTCTGTATCAGAAGATTTTCAAGGGTGACACTCAGATCCCAAAGTGGCTTTCACACAGTGCACAAAATCTTCTTCGGAGGATTCTTGAACCCAATCCGATGAAGAGGATCGACATGGCAGGGATCAAATCACACGAATGGTTTCAGAAGGACTATATTCCTGTTCTTCCATATGATGATGACGATGAAGATGTACAGTTTGGGGCACGTCTTCCTGCCAAAGAGCAAATCAATGATGAGCCTGGAGATAAGAATTCTCATCAGATCAACGCTTTCCAGTTGATTGGAATGGCATCATCCCTTGATCTTTCAGGGTTTTTCGAGGATGAGGAAGTGTCCCAAAGGAGGATTAGATTCACATCAACGCATCCACCCAAGGATGCCTTCGACAAGATTGAAAGCTCAGCGACGGAGTTGGGATTCCAAGTTCAGAGGGGGCACAGCAAGCTCAAATTAATGCGCAATTGCAAGGGATCAAAGAACCCTGAGTCATTTATGGTGTCCGCCGAGGTTTTTGAGCTTGGCCCTTCTGTAAATGTTGTAGAACTTAGGAAGTCCAACGGAGACCCTGCACTGTATAGACAGCTCTGTGAGAGGATCTCCAGTGACATGGGGGCGCGCAACACGGAGCAGATCTTCGCAACGGCATCGCTTGAGGATGATCTTCAGAACAGCAATGCAGGGACGCCGCTGTTCGCTTTGTAACGAATGCTGTGTTCTGCAAAACTGCAATGTTGTAGGATGATCTTAGGCAAGAGGATGTGTAGGAACATATTTGGTCAGGCATTTGGAGAAGAGATAAGACTTGAGAGGGTAGGATCATAGTAGTATATGATACAGTGTACAGAACCGAGTGGTTCAAAAACTAGCCACCGTTGTAAAGAGAAAGAAAGGAAAGATGAAATAAAAGAAAGAAATAAATTGCCTTGA
CDS Sequence
- >LOC_Os05g04550.1
ATGGTGAAGGGAGGGAGGGAGGCGCTGCTGGGCGGGTACGAGATGGGGAGGACGCTCGGGGAGGGCAACTTCGGCAAGGTGAAGTACGCGCGCCACCTCGCCACCGGCGGCCACTTCGCCGTCAAGATCCTCGACCGCGGCCGCGTCGTCTCCCTCCGCGCCGGCGACCAGATTCGCCGCGAGATCGCCACGCTCAAGCTGCTCAGGCACCCCCACGTCGTCCGCCTCCACGAGGTTGCTGCTAGCAAAACTAAGATTTACATGGTGCTTGAGTTTGTCAATGGAGGTGAACTGTTTGAGAGAATTGCAGTAAAGGGAAAACTATCAGAGAAAGAAGGAAGGAGGCTTTTTCAGCAGTTAATTGATGGTGTTAGCTATTGCCATGATAGGGGAGTCTACCACAGAGACCTCAAGCCTGAGAACGTTCTTGTGGACCAAAAAGGCAACATCAAGATCTCTGATTTTGGTCTTAGTGCTTTACCTCAACACCTCGGGAATGATGGATTGCTGCATACTACCTGTGGTAGCCCCAACTATATTGCTCCTGAGGTTCTGCAGAATAAAGGATATGATGGATCCTTGTCAGATATCTGGTCTTGCGGAGTAATCCTTTATGTAATGCTTATAGGATATCTTCCGTTCGATGACCGAAATATTGTTGTTCTGTATCAGAAGATTTTCAAGGGTGACACTCAGATCCCAAAGTGGCTTTCACACAGTGCACAAAATCTTCTTCGGAGGATTCTTGAACCCAATCCGATGAAGAGGATCGACATGGCAGGGATCAAATCACACGAATGGTTTCAGAAGGACTATATTCCTGTTCTTCCATATGATGATGACGATGAAGATGTACAGTTTGGGGCACGTCTTCCTGCCAAAGAGCAAATCAATGATGAGCCTGGAGATAAGAATTCTCATCAGATCAACGCTTTCCAGTTGATTGGAATGGCATCATCCCTTGATCTTTCAGGGTTTTTCGAGGATGAGGAAGTGTCCCAAAGGAGGATTAGATTCACATCAACGCATCCACCCAAGGATGCCTTCGACAAGATTGAAAGCTCAGCGACGGAGTTGGGATTCCAAGTTCAGAGGGGGCACAGCAAGCTCAAATTAATGCGCAATTGCAAGGGATCAAAGAACCCTGAGTCATTTATGGTGTCCGCCGAGGTTTTTGAGCTTGGCCCTTCTGTAAATGTTGTAGAACTTAGGAAGTCCAACGGAGACCCTGCACTGTATAGACAGCTCTGTGAGAGGATCTCCAGTGACATGGGGGCGCGCAACACGGAGCAGATCTTCGCAACGGCATCGCTTGAGGATGATCTTCAGAACAGCAATGCAGGGACGCCGCTGTTCGCTTTGTAA
Protein Sequence
- >LOC_Os05g04550.1
MVKGGREALLGGYEMGRTLGEGNFGKVKYARHLATGGHFAVKILDRGRVVSLRAGDQIRREIATLKLLRHPHVVRLHEVAASKTKIYMVLEFVNGGELFERIAVKGKLSEKEGRRLFQQLIDGVSYCHDRGVYHRDLKPENVLVDQKGNIKISDFGLSALPQHLGNDGLLHTTCGSPNYIAPEVLQNKGYDGSLSDIWSCGVILYVMLIGYLPFDDRNIVVLYQKIFKGDTQIPKWLSHSAQNLLRRILEPNPMKRIDMAGIKSHEWFQKDYIPVLPYDDDDEDVQFGARLPAKEQINDEPGDKNSHQINAFQLIGMASSLDLSGFFEDEEVSQRRIRFTSTHPPKDAFDKIESSATELGFQVQRGHSKLKLMRNCKGSKNPESFMVSAEVFELGPSVNVVELRKSNGDPALYRQLCERISSDMGARNTEQIFATASLEDDLQNSNAGTPLFAL*