Information report for OsSAMS1
Gene Details
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Functional Descriptions
- Our results suggest that OsFBK12 is involved in the 26S proteasome pathway by interacting with Oryza sativa S-PHASE KINASE-ASSOCIATED PROTEIN1-LIKE PROTEIN and that it targets the substrate OsSAMS1 for degradation, triggering changes in ethylene levels for the regulation of leaf senescence and grain size.
- Phenotypically, overexpression of OsFBK12 led to a delay in leaf senescence and germination and increased seed size, whereas knockdown lines of either OsFBK12 or OsSAMS1 promoted the senescence program.
- The F-box protein OsFBK12 targets OsSAMS1 for degradation and affects pleiotropic phenotypes, including leaf senescence, in rice.
- Here, we demonstrate that OsSAMS1, 2 and 3 are essential for histone H3K4me3 and DNA methylation to regulate gene expression related to flowering in Oryza sativa.
- RNA interference (RNAi) transgenic rice with downregulated transcripts of OsSAMS1, 2 and 3 showed pleiotropic phenotypes, including dwarfism, reduced fertility, delayed germination, as well as late flowering.
- Knockdown of OsSAMS1, 2 and 3 led to distinguished late flowering and greatly reduced the expression of the flowering key genes, Early heading date 1 (Ehd1), Hd3a and RFT1 (rice FT-like genes).
- OsFBK12-RNA interference lines and OsSAMS1 overexpression lines showed increased ethylene levels, while OsFBK12-OX lines and OsSAMS1-RNA interference plants exhibited decreased ethylene.
- RDV-encoded Pns11 protein specifically interacted with OsSAMS1 to enhance its enzymatic activity, leading to higher ethylene levels in both RDV-infected and Pns11-overexpressing rice.
Functional Keywords
- grain-size , leaf , flower , senescence , dwarf , ethylene , heading-date , fertility , seed-size , seed , grain
Literature and News
- Knockdown of SAMS genes encoding S-adenosyl-l-methionine synthetases causes methylation alterations of DNAs and histones and leads to late flowering in rice . DOI: 10.1016/j.jplph.2011.05.020 ; PMID: 21757254
- The F-box protein OsFBK12 targets OsSAMS1 for degradation and affects pleiotropic phenotypes, including leaf senescence, in rice . DOI: 10.1104/pp.113.224527 ; PMID: 24144792
- A viral protein promotes host SAMS1 activity and ethylene production for the benefit of virus infection . DOI: 10.7554/eLife.27529 ; PMID: 28994391
Gene Resources
- UniProt: Q0DKY4
- EMBL: AC087552, AK072051, AP008211
- AlphaFoldDB: Q0DKY4
- EnsemblPlants: Os05t0135700-01, Os05t0135700-02
- Gramene: Os05t0135700-01, Os05t0135700-02
- KEGG: dosa:Os05g0135700
- Orthologous matrix: YINPCGR
- InterPro: IPR002133, IPR022628, IPR022629
Sequences
cDNA Sequence
- >LOC_Os05g04510.1
ATCTCGCCGTGCCCGGTCGGATCTAATTAATTTACCTCCTCCACCTCCTCCTCCTCCCCCCGATCGGATCGGCCCGGCTCCTCCTCCTCCGCCGCCGCCACCGCCGCCGCAGATAGGAGAAGATGGCCGCACTTGATACCTTCCTCTTTACCTCGGAGTCTGTGAACGAGGGCCACCCTGACAAGCTCTGCGACCAAGTCTCAGATGCTGTGCTTGATGCCTGCCTCGCCGAGGACCCTGACAGCAAGGTCGCTTGTGAGACCTGCACCAAGACAAACATGGTCATGGTCTTTGGTGAGATCACCACCAAGGCTAACGTTGACTATGAGAAGATTGTCAGGGAGACATGCCGTAACATCGGTTTTGTGTCAGCTGATGTCGGTCTCGATGCTGACCACTGCAAGGTGCTTGTGAACATCGAGCAGCAGTCCCCTGACATTGCACAGGGTGTGCACGGCCACTTCACCAAGCGCCCTGAGGAGATTGGTGCTGGTGACCAGGGACACATGTTTGGATATGCAACTGATGAGACCCCTGAGTTGATGCCCCTCAGCCATGTCCTTGCTACCAAGCTTGGCGCTCGTCTTACGGAGGTTCGCAAGAATGGGACCTGCGCATGGCTCAGGCCTGACGGGAAGACCCAAGTGACTGTTGAGTACCGCAATGAGAGCGGTGCCAGGGTCCCTGTCCGTGTCCACACCGTCCTCATCTCTACCCAGCATGATGAGACAGTCACCAACGATGAGATTGCTGCTGACCTGAAGGAGCATGTCATCAAGCCTGTCATTCCCGAGCAGTACCTTGATGAGAAGACAATCTTCCATCTTAACCCATCTGGTCGCTTCGTCATTGGCGGACCTCATGGTGATGCTGGTCTCACTGGCCGGAAGATCATCATTGACACTTATGGTGGCTGGGGAGCTCACGGTGGTGGTGCCTTCTCTGGCAAGGACCCAACCAAGGTTGACCGCAGTGGAGCATACGTCGCAAGGCAAGCTGCCAAGAGCATTGTTGCTAGTGGCCTTGCTCGCCGCTGCATTGTCCAAGTATCATACGCCATCGGTGTCCCAGAGCCACTGTCCGTATTCGTCGACACATACGGCACTGGCAGGATCCCTGACAAGGAGATCCTCAAGATTGTGAAGGAGAACTTCGACTTCAGGCCTGGCATGATCATCATCAACCTTGACCTCAAGAAAGGCGGCAACGGACGCTACCTCAAGACGGCGGCTTACGGTCACTTCGGAAGGGACGACCCAGACTTCACCTGGGAGGTGGTGAAGCCCCTCAAGTGGGAGAAGCCTTCTGCCTAAAAGCTCCCTTTCGGAGGCTTTTGCTCTGTCCCATTATGGTGTTTTGTTTCCTCGCTGCTCAGCATTGTGATTCTTAACCTGCCCCCCGCTGCCATTTATGCCCATGCACGCTACTTTCCTAATAATAAGTACTTATAAGGGTATTGTGTTTGAATATTTTACCTAGAGGAGGAGGAGGATTTGTTATCTGTTATTGCTTAAGCTTATGTAATGGTGAAAGAAGGAAAGATGCTACACTACTTTGAGAAGAAGAAATGAAACCTCCTATGTTTTGGTTATTCTATCCCTATATGGTTCCCTGGTGATCTGTGAGTGCTCAGTTCATGTGTTTGCTGAAGCTGGAAGTGACCTTTGTGCCTTTTGAAGTTGAGCATGCAACATGTTTGTTGGTCCATGATGATACCACGCCCTTTAGGACTCTTGCTTTTGCTATTCCAACTGAGACTTGTTGATTGATGCTGTACACATGTTGAATGTTAGAAGTCTGATGGTGGTTGGTGATCAAACTAAATAAGGAGGGTTTAGATGTCACTAGCAGCTCAGAGTGCATCCAAGGTAAGCTGTCGATTTCACTGACAGCTCAGAATGCATCCCATAAAATCGGTTGTCCAAGTCGCTGGCAATCCAACTTGTCATGCTACTAGCTTGGAGTTTAAAGAAGATGCTTTTGGTT - >LOC_Os05g04510.2
ATCTCGCCGTGCCCGGTCGGATCTAATTAATTTACCTCCTCCACCTCCTCCTCCTCCCCCCGATCGGATCGGCCCGGCTCCTCCTCCTCCGCCGCCGCCACCGCCGCCGCAGATAGGAGAAGATGGCCGCACTTGATACCTTCCTCTTTACCTCGGAGTCTGTGAACGAGGGCCACCCTGACAAGCTCTGCGACCAAGTCTCAGATGCTGTGCTTGATGCCTGCCTCGCCGAGGACCCTGACAGCAAGGTCGCTTGTGAGACCTGCACCAAGACAAACATGGTCATGGTCTTTGGTGAGATCACCACCAAGGCTAACGTTGACTATGAGAAGATTGTCAGGGAGACATGCCGTAACATCGGTTTTGTGTCAGCTGATGTCGGTCTCGATGCTGACCACTGCAAGGTGCTTGTGAACATCGAGCAGCAGTCCCCTGACATTGCACAGGGTGTGCACGGCCACTTCACCAAGCGCCCTGAGGAGATTGGTGCTGGTGACCAGGGACACATGTTTGGATATGCAACTGATGAGACCCCTGAGTTGATGCCCCTCAGCCATGTCCTTGCTACCAAGCTTGGCGCTCGTCTTACGGAGGTTCGCAAGAATGGGACCTGCGCATGGCTCAGGCCTGACGGGAAGACCCAAGTGACTGTTGAGTACCGCAATGAGAGCGGTGCCAGGGTCCCTGTCCGTGTCCACACCGTCCTCATCTCTACCCAGCATGATGAGACAGTCACCAACGATGAGATTGCTGCTGACCTGAAGGAGCATGTCATCAAGCCTGTCATTCCCGAGCAGTACCTTGATGAGAAGACAATCTTCCATCTTAACCCATCTGGTCGCTTCGTCATTGGCGGACCTCATGGTGATGCTGGTCTCACTGGCCGGAAGATCATCATTGACACTTATGGTGGCTGGGGAGCTCACGGTGGTGGTGCCTTCTCTGGCAAGGACCCAACCAAGGTTGACCGCAGTGGAGCATACGTCGCAAGGCAAGCTGCCAAGAGCATTGTTGCTAGTGGCCTTGCTCGCCGCTGCATTGTCCAAGTATCATACGCCATCGGTGTCCCAGAGCCACTGTCCGTATTCGTCGACACATACGGCACTGGCAGGATCCCTGACAAGGAGATCCTCAAGATTGTGAAGGAGAACTTCGACTTCAGGCCTGGCATGATCATCATCAACCTTGACCTCAAGAAAGGCGGCAACGGACGCTACCTCAAGACGGCGGCTTACGGTCACTTCGGAAGGGACGACCCAGACTTCACCTGGGAGGTGGTGAAGCCCCTCAAGTGGGAGAAGCCTTCTGCCTAAAAGCTCCCTTTCGGAGGCTTTTGCTCTGTCCCATTATGGTGTTTTGTTTCCTCGCTGCTCAGCATTGTGATTCTTAACCTGCCCCCCGCTGCCATTTATGCCCATGCACGCTACTTTCCTAATAATAAGTACTTATAAGGGTATTGTGTTTGAATATTTTACCTAGAGGAGGAGGAGGATTTGTTATCTGTTATTGCTTAAGCTTATGTAATGGTGAAAGAAGGAAAGATGCTACACTACTTTGAGAAGAAGAAATGAAACCTCCTATGTTTTGGTTATTCTATCCCTATATGGTTCCCTGGTGATCTGTGAGTGCTCAGTTCATGTGTTTGCTGAAGCTGGAAGTGACCTTTGTGCCTTTTGAAGTTGAGCATGCAACATGTTTGTTGGTCCATGATGATACCACGCCCTTTAGGACTCTTGCTTTTGCTATTCCAACTGAGACTTGTTGATTGATGCTGTACACATGTTGAATGTTAGAAGTCTGATGGTGGTTGGTGATCAAACTAAATAAGGAGGGTTTAGATGTCACTAGCAGCTCAGAGTGCATCCAAGGTAAGCTGTCGATTTCACTGACAGCTCAGAATGCATCCCATAAAATCGGTTGTCCAAGTCGCTGGCAATCCAACTTGTCATGCTACTAGCTTGGAGTTTAAAGAAGATGCTTTTGGTT
CDS Sequence
- >LOC_Os05g04510.1
ATGGCCGCACTTGATACCTTCCTCTTTACCTCGGAGTCTGTGAACGAGGGCCACCCTGACAAGCTCTGCGACCAAGTCTCAGATGCTGTGCTTGATGCCTGCCTCGCCGAGGACCCTGACAGCAAGGTCGCTTGTGAGACCTGCACCAAGACAAACATGGTCATGGTCTTTGGTGAGATCACCACCAAGGCTAACGTTGACTATGAGAAGATTGTCAGGGAGACATGCCGTAACATCGGTTTTGTGTCAGCTGATGTCGGTCTCGATGCTGACCACTGCAAGGTGCTTGTGAACATCGAGCAGCAGTCCCCTGACATTGCACAGGGTGTGCACGGCCACTTCACCAAGCGCCCTGAGGAGATTGGTGCTGGTGACCAGGGACACATGTTTGGATATGCAACTGATGAGACCCCTGAGTTGATGCCCCTCAGCCATGTCCTTGCTACCAAGCTTGGCGCTCGTCTTACGGAGGTTCGCAAGAATGGGACCTGCGCATGGCTCAGGCCTGACGGGAAGACCCAAGTGACTGTTGAGTACCGCAATGAGAGCGGTGCCAGGGTCCCTGTCCGTGTCCACACCGTCCTCATCTCTACCCAGCATGATGAGACAGTCACCAACGATGAGATTGCTGCTGACCTGAAGGAGCATGTCATCAAGCCTGTCATTCCCGAGCAGTACCTTGATGAGAAGACAATCTTCCATCTTAACCCATCTGGTCGCTTCGTCATTGGCGGACCTCATGGTGATGCTGGTCTCACTGGCCGGAAGATCATCATTGACACTTATGGTGGCTGGGGAGCTCACGGTGGTGGTGCCTTCTCTGGCAAGGACCCAACCAAGGTTGACCGCAGTGGAGCATACGTCGCAAGGCAAGCTGCCAAGAGCATTGTTGCTAGTGGCCTTGCTCGCCGCTGCATTGTCCAAGTATCATACGCCATCGGTGTCCCAGAGCCACTGTCCGTATTCGTCGACACATACGGCACTGGCAGGATCCCTGACAAGGAGATCCTCAAGATTGTGAAGGAGAACTTCGACTTCAGGCCTGGCATGATCATCATCAACCTTGACCTCAAGAAAGGCGGCAACGGACGCTACCTCAAGACGGCGGCTTACGGTCACTTCGGAAGGGACGACCCAGACTTCACCTGGGAGGTGGTGAAGCCCCTCAAGTGGGAGAAGCCTTCTGCCTAA - >LOC_Os05g04510.2
ATGGCCGCACTTGATACCTTCCTCTTTACCTCGGAGTCTGTGAACGAGGGCCACCCTGACAAGCTCTGCGACCAAGTCTCAGATGCTGTGCTTGATGCCTGCCTCGCCGAGGACCCTGACAGCAAGGTCGCTTGTGAGACCTGCACCAAGACAAACATGGTCATGGTCTTTGGTGAGATCACCACCAAGGCTAACGTTGACTATGAGAAGATTGTCAGGGAGACATGCCGTAACATCGGTTTTGTGTCAGCTGATGTCGGTCTCGATGCTGACCACTGCAAGGTGCTTGTGAACATCGAGCAGCAGTCCCCTGACATTGCACAGGGTGTGCACGGCCACTTCACCAAGCGCCCTGAGGAGATTGGTGCTGGTGACCAGGGACACATGTTTGGATATGCAACTGATGAGACCCCTGAGTTGATGCCCCTCAGCCATGTCCTTGCTACCAAGCTTGGCGCTCGTCTTACGGAGGTTCGCAAGAATGGGACCTGCGCATGGCTCAGGCCTGACGGGAAGACCCAAGTGACTGTTGAGTACCGCAATGAGAGCGGTGCCAGGGTCCCTGTCCGTGTCCACACCGTCCTCATCTCTACCCAGCATGATGAGACAGTCACCAACGATGAGATTGCTGCTGACCTGAAGGAGCATGTCATCAAGCCTGTCATTCCCGAGCAGTACCTTGATGAGAAGACAATCTTCCATCTTAACCCATCTGGTCGCTTCGTCATTGGCGGACCTCATGGTGATGCTGGTCTCACTGGCCGGAAGATCATCATTGACACTTATGGTGGCTGGGGAGCTCACGGTGGTGGTGCCTTCTCTGGCAAGGACCCAACCAAGGTTGACCGCAGTGGAGCATACGTCGCAAGGCAAGCTGCCAAGAGCATTGTTGCTAGTGGCCTTGCTCGCCGCTGCATTGTCCAAGTATCATACGCCATCGGTGTCCCAGAGCCACTGTCCGTATTCGTCGACACATACGGCACTGGCAGGATCCCTGACAAGGAGATCCTCAAGATTGTGAAGGAGAACTTCGACTTCAGGCCTGGCATGATCATCATCAACCTTGACCTCAAGAAAGGCGGCAACGGACGCTACCTCAAGACGGCGGCTTACGGTCACTTCGGAAGGGACGACCCAGACTTCACCTGGGAGGTGGTGAAGCCCCTCAAGTGGGAGAAGCCTTCTGCCTAA
Protein Sequence
- >LOC_Os05g04510.1
MAALDTFLFTSESVNEGHPDKLCDQVSDAVLDACLAEDPDSKVACETCTKTNMVMVFGEITTKANVDYEKIVRETCRNIGFVSADVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEEIGAGDQGHMFGYATDETPELMPLSHVLATKLGARLTEVRKNGTCAWLRPDGKTQVTVEYRNESGARVPVRVHTVLISTQHDETVTNDEIAADLKEHVIKPVIPEQYLDEKTIFHLNPSGRFVIGGPHGDAGLTGRKIIIDTYGGWGAHGGGAFSGKDPTKVDRSGAYVARQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDTYGTGRIPDKEILKIVKENFDFRPGMIIINLDLKKGGNGRYLKTAAYGHFGRDDPDFTWEVVKPLKWEKPSA* - >LOC_Os05g04510.2
MAALDTFLFTSESVNEGHPDKLCDQVSDAVLDACLAEDPDSKVACETCTKTNMVMVFGEITTKANVDYEKIVRETCRNIGFVSADVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEEIGAGDQGHMFGYATDETPELMPLSHVLATKLGARLTEVRKNGTCAWLRPDGKTQVTVEYRNESGARVPVRVHTVLISTQHDETVTNDEIAADLKEHVIKPVIPEQYLDEKTIFHLNPSGRFVIGGPHGDAGLTGRKIIIDTYGGWGAHGGGAFSGKDPTKVDRSGAYVARQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDTYGTGRIPDKEILKIVKENFDFRPGMIIINLDLKKGGNGRYLKTAAYGHFGRDDPDFTWEVVKPLKWEKPSA*