Gene Details:

• MSU gene ID: LOC_Os04g57610
• RAPdb gene ID: Os04g0671900
• Gene Symbol: OsARF12
• Genome: MSU7 ,  IRGSP-1.0
• Species: Oryza sativa

Functional Descriptions:

• Here, we found a limiting factor of RSA–OsARF12–an auxin response factor whose knockout led to decreased primary root length in rice (Oryza sativa).
• • The root elongation zones of OsARF12 and OsARF12/25, which had lower auxin concentrations, were distinctly shorter than for the wild-type, possibly as a result of decreased expression of auxin synthesis genes OsYUCCAs and auxin efflux carriers OsPINs and OsPGPs.
• The knockout of OsARF12 also altered the abundance of mitochondrial iron-regulated (OsMIR), iron (Fe)-regulated transporter1 (OsIRT1) and short postembryonic root1 (OsSPR1) in roots of rice, and resulted in lower Fe content.
• • The data provide evidence for the biological function of OsARF12, which is implicated in regulating root elongation.
• OsARF12, a transcription activator on auxin response gene, regulates root elongation and affects iron accumulation in rice (Oryza sativa).
• Transcript analysis revealed that Pi-responsive genes–Phosphate starvation (OsIPS)1 and OsIPS2, SYG1/Pho81/XPR1(OsSPX1), Sulfoquinovosyldiacylglycerol 2 (OsSQD2), R2R3 MYB transcription factor (OsMYB2P-1) and Transport Inhibitor Response1 (OsTIR1)–were more abundant in the OsARF12 and OsARF12/25 mutants under +Pi/-Pi conditions.
• Auxin response factor (OsARF12), a novel regulator for phosphate homeostasis in rice (Oryza sativa).
• Here, we report that an auxin response factor, OsARF12, functions in Pi homeostasis.
• Results from -Pi/1-naphthylphthalamic acid (NPA) treatments, and auxin reporter DR5::GUS staining suggest that root system alteration and Pi-induced auxin response were at least partially controlled by OsARF12.
• • OsARF12 as a transcription activator can facilitate the expression of the auxin response element DR5::GFP, and OsARF12 was inhibited by osa-miRNA167d by transient expression in tobacco and rice callus.
• These findings enrich our understanding of the biological functions of OsARF12, which also acts in regulating Pi homeostasis.
• Measurement of element content, quantitative reverse transcription polymerase chain reaction analysis and acid phosphatases (APases) activity assay showed that the OsARF12 mutant and OsARF12/25 double mutant with P-intoxicated phenotypes had higher P concentrations, up-regulation of the Pi transporter encoding genes and increased APase activity under Pi-sufficient/-deficient (+Pi/-Pi, 0.
• Strikingly, our genetic data showed that loss-of-function mutants of OsARF12 or osarf16 exhibit reduced resistance whereas osarf11 mutants display enhanced resistance to RDV.
• In turn, OsARF12 activates the down-stream OsWRKY13 expression through direct binding to its promoter, loss-of-function mutants of oswrky13 exhibit reduced resistance.
• miR167d-ARFs Module Regulates Flower Opening and Stigma Size in Rice.
• Further analysis indicates that OsARF12 is the major mediator of miR167 in regulating rice grain filling.
• Expectedly, over expressing OsARF12 could resemble the phenotype of STTM/MIM167 plants with respect to grain weight and grain filling rate.
• Flow cytometric analysis in young panicles of plants overexpressing OsARF12 and cell number examination of cdkf;2 mutants verify that OsARF12 positively regulates grain filling and grain size by targeting OsCDKF;2.
• Additionally, plants overexpressing OsARF12 or cdkf;2 mutants present enhanced or reduced sensitivity to exogenous auxin and brassinosteroid (BR) treatments, confirming that OsCDKF;2 targeting by OsARF12 mediates auxin and BR signaling.

Function-related keywords:

• primary-root ,  root ,  phosphate ,  auxin ,  iron ,  homeostasis ,  mitochondria ,  transporter ,  transcription-factor ,  pi ,  resistance ,  flower-opening ,  stigma-size ,  grain ,  grain-size ,  brassinosteroid ,  BR ,  Brassinosteroid ,  BR-signaling ,  grain-filling ,  grain-weight ,  young-panicles

Literature:

• OsARF12, a transcription activator on auxin response gene, regulates root elongation and affects iron accumulation in rice (Oryza sativa) .  DOI:  10.1111/j.1469-8137.2011.03910.x ;  PMID:  21973088
• Auxin response factor (OsARF12), a novel regulator for phosphate homeostasis in rice (Oryza sativa) .  DOI:  10.1111/nph.12499 ;  PMID:  24111723
• Osa-miR167d facilitates infection of Magnaporthe oryzae in rice .  DOI:  10.1111/jipb.12816 ;  PMID:  31001874
• Rice GROWTH-REGULATING FACTOR7 Modulates Plant Architecture through Regulating GA and IAA Metabolism .  DOI:  10.1104/pp.20.00302 ;  PMID:  32581114
• Auxin response factors (ARFs) differentially regulate rice antiviral immune response against rice dwarf virus .  DOI:  10.1371/journal.ppat.1009118 ;  PMID:  33264360
• The miR167-OsARF12 module regulates rice grain filling and grain size downstream of miR159 .  DOI:  10.1016/j.xplc.2023.100604 ;  PMID:  37085993

Related News:

Gene Resources:

• NCBI ID: AK071455
• UniProt accessions:

Sequences: