Gene Details:
- MSU gene ID: LOC_Os04g56580
- RAPdb gene ID: Os04g0661200
- Gene Symbol: IPK1 OsIPK1
- Genome: MSU7 , IRGSP-1.0
- Species: Oryza sativa
Functional Descriptions:
- 85-fold down-regulation in IPK1 transcripts, which correlated to a significant reduction in phytate levels and a concomitant increase in the amount of inorganic phosphate (Pi).
- Development of low phytate rice by RNAi mediated seed-specific silencing of inositol 1,3,4,5,6-pentakisphosphate 2-kinase gene (IPK1).
- The results provide evidence that silencing of IPK1 gene can mediate a substantial reduction in seed phytate levels without hampering the growth and development of transgenic rice plants.
- In the present study, we report the RNAi-mediated seed-specific silencing (using the Oleosin18 promoter) of the IPK1 gene, which catalyzes the last step of phytic acid biosynthesis in rice.
Function-related keywords:
Literature:
- Development of low phytate rice by RNAi mediated seed-specific silencing of inositol 1,3,4,5,6-pentakisphosphate 2-kinase gene (IPK1) . DOI: 10.1371/journal.pone.0068161 ; PMID: 23844166
- An Inositol 1, 3, 4, 5, 6-Pentakisphosphate 2-Kinase 1 Mutant with a 33-nt Deletion Showed Enhanced Tolerance to Salt and Drought Stress in Rice . DOI: 10.3390/plants10010023 ; PMID: 33374298
Related News:
Gene Resources:
- NCBI ID: AK102842
- UniProt accessions:
Sequences:
cDNA Sequence
- >LOC_Os04g56580.1
GCTAATCCTTCCTTGCAGGGGAGGGGAATCTGCCGCTTTCCTCATCGTCGGCTCCACTTCCACTTCCTCTTCCTCCTCCTCGAGGACGAGGAGGAAATTGGCAGCTGGAGGCTTGGAGCGCGCTGCATCATCCCGCCCGCCCTGCTGCTGCGGCGATCGCCGGAGCAGGGCTGCAGGGGATCCCCACGGCCGCAGCAGCTGTTGTTATCTGATTCTGTGTGGGGATGGAGGTGGTTCTGCATGAGGGGGATGCCAAAGATTGGGTTTACAAAGGAGAAGGTGCAGCGAATCTAATCCTCAGCTACACTGGCTCGTCACCTTCTATGCTTGGCAAGGTTCTGCGAGTCAAAAAGATTCTAAAAGACAAGGGACAACCGGCACCAAACTGTATAGTCTTCTCAAGTCATGAGGAACATCTGTGGGGCAAGATCCCAGGATTGTTGGAATCTGTTAAAAATGATTGCTTGCCACAAGCCTATGCTACAATTGTTATGAGCCAACATTTGGGTGCCAATCATGTTGATGGCGGGGTCCGTGTACGTGTGTCTAAGAACTTTTTCGAGCTTGCTGGAAAGAATGTGCTTGACAACCGTCCTGCATGGAGAGTGAATGCTAGTGCAATTGATGCTGGAGCTGATTCTGCTCTTCTAATTTCTGACCACACATTATTTTCCGGTAATCCTAGAGGAAGCAGTTGCATAGCAGTGGAGATAAAGGCAAAATGTGGATTTCTTCCGTCATCTGAATACATATCGAAGGAGAATTCTATCAAGAAACAAGTAACACGGTATAAGATGCATCAGCACCTAAAATTTCATCTGGGAGAGATATCGAAGACAAGTGAATACGATCCCCTAGATTTATTTTCTGGATCAAAAGAAAGAATACATATGGCTATCAAGTCATTTTTTTCAACTCCTCAGAACAACTTTAGAATTTTTGTGGATGGTTCTTTAGTTTTTGGTGGCATGGGAGGTGGCGCAGATAGTGTTCATCCTAATGAAACAGAGAAATGTCTTGAAGATCTGAGCAAGGTTACTGGCTTACAACTATCTGACTTTATTGAGCTCCTGTCAGAGGCAATCTTTAAGTCTGGAGTGTTGGGTAAACTTTTAGCCACTCAAAAACTGGATGATCATGACATCGAAGGGGCGATTCATCTGTACTATAACATCATTTCTCAGCCTTGTTTGGTATGCAAAAGTATAACTGATACAGAACTTCTGCGCAAGTACTCCACCTTGCATTCTCTTCCATTGGACAAGAGTGAGAAGATTGTCAGGGACTTTCTTATCTCTGCTACTGCAAAAGATTGTAGCCTAATGATCAGCTTTCGACCAAGACAGAGTGGAACAACAGATTCTGAGTACGATTCTGTATTTCTTGATTCAGTGAACCAAAGCTATGATTACAAGGCATATTTTATTGATCTGGATGTGAAACCCTTGGATAAGATGGTACATTACTTTAAATTGGATCAAAAGATAGTCAATTTCTACACTAGAAATGGAGAAGTTGGGGGAGATCCACGTGATCCTCCAAAGGGATGTGGCCCTAGGTGACACAAAGGTTCAGCTCCAAAGGTTCAGCTCCAACATTGACGACCTTGAAATATAAAGGGCATAGGACAAGTGTCTGTTGTATCTTGGTGATTGTTGTGTTTCTACAAGTGTGCATGTCCAATGTGCAGGTTCTCCTCCCTTCTAGGGAAAACGTGTAAGAGAGTGGTAGTTGTTGTAGTACATCCTGTATACCGTGTGGTTTGCCTCAGCAGTAGGCCGAATTTTCAGTAGACAAACATTAAGAAGAGTTAATAAACTGTATACTGAGGCATCCACGTTTACCTTGGAAATCTATGATAAATTCCCGTATACTCAGCTTGCTGTGAATCTGTGATCTGAGTTCTGAC
CDS Sequence
- >LOC_Os04g56580.1
ATGGAGGTGGTTCTGCATGAGGGGGATGCCAAAGATTGGGTTTACAAAGGAGAAGGTGCAGCGAATCTAATCCTCAGCTACACTGGCTCGTCACCTTCTATGCTTGGCAAGGTTCTGCGAGTCAAAAAGATTCTAAAAGACAAGGGACAACCGGCACCAAACTGTATAGTCTTCTCAAGTCATGAGGAACATCTGTGGGGCAAGATCCCAGGATTGTTGGAATCTGTTAAAAATGATTGCTTGCCACAAGCCTATGCTACAATTGTTATGAGCCAACATTTGGGTGCCAATCATGTTGATGGCGGGGTCCGTGTACGTGTGTCTAAGAACTTTTTCGAGCTTGCTGGAAAGAATGTGCTTGACAACCGTCCTGCATGGAGAGTGAATGCTAGTGCAATTGATGCTGGAGCTGATTCTGCTCTTCTAATTTCTGACCACACATTATTTTCCGGTAATCCTAGAGGAAGCAGTTGCATAGCAGTGGAGATAAAGGCAAAATGTGGATTTCTTCCGTCATCTGAATACATATCGAAGGAGAATTCTATCAAGAAACAAGTAACACGGTATAAGATGCATCAGCACCTAAAATTTCATCTGGGAGAGATATCGAAGACAAGTGAATACGATCCCCTAGATTTATTTTCTGGATCAAAAGAAAGAATACATATGGCTATCAAGTCATTTTTTTCAACTCCTCAGAACAACTTTAGAATTTTTGTGGATGGTTCTTTAGTTTTTGGTGGCATGGGAGGTGGCGCAGATAGTGTTCATCCTAATGAAACAGAGAAATGTCTTGAAGATCTGAGCAAGGTTACTGGCTTACAACTATCTGACTTTATTGAGCTCCTGTCAGAGGCAATCTTTAAGTCTGGAGTGTTGGGTAAACTTTTAGCCACTCAAAAACTGGATGATCATGACATCGAAGGGGCGATTCATCTGTACTATAACATCATTTCTCAGCCTTGTTTGGTATGCAAAAGTATAACTGATACAGAACTTCTGCGCAAGTACTCCACCTTGCATTCTCTTCCATTGGACAAGAGTGAGAAGATTGTCAGGGACTTTCTTATCTCTGCTACTGCAAAAGATTGTAGCCTAATGATCAGCTTTCGACCAAGACAGAGTGGAACAACAGATTCTGAGTACGATTCTGTATTTCTTGATTCAGTGAACCAAAGCTATGATTACAAGGCATATTTTATTGATCTGGATGTGAAACCCTTGGATAAGATGGTACATTACTTTAAATTGGATCAAAAGATAGTCAATTTCTACACTAGAAATGGAGAAGTTGGGGGAGATCCACGTGATCCTCCAAAGGGATGTGGCCCTAGGTGA
Protein Sequence
- >LOC_Os04g56580.1
MEVVLHEGDAKDWVYKGEGAANLILSYTGSSPSMLGKVLRVKKILKDKGQPAPNCIVFSSHEEHLWGKIPGLLESVKNDCLPQAYATIVMSQHLGANHVDGGVRVRVSKNFFELAGKNVLDNRPAWRVNASAIDAGADSALLISDHTLFSGNPRGSSCIAVEIKAKCGFLPSSEYISKENSIKKQVTRYKMHQHLKFHLGEISKTSEYDPLDLFSGSKERIHMAIKSFFSTPQNNFRIFVDGSLVFGGMGGGADSVHPNETEKCLEDLSKVTGLQLSDFIELLSEAIFKSGVLGKLLATQKLDDHDIEGAIHLYYNIISQPCLVCKSITDTELLRKYSTLHSLPLDKSEKIVRDFLISATAKDCSLMISFRPRQSGTTDSEYDSVFLDSVNQSYDYKAYFIDLDVKPLDKMVHYFKLDQKIVNFYTRNGEVGGDPRDPPKGCGPR*