OsHKT1;4

Gene Details

MSU gene ID: LOC_Os04g51830
RAPdb gene ID: Os04g0607600
Gene Symbol: OsHKT1 4
Genome: MSU7 , IRGSP-1.0
Species: Oryza sativa

Functional Descriptions

For OsHKT1;4, alternative splicing of transcript and the anatomical complexity of the sheath needed to be taken into account.
Thus, Na(+) accumulation in a specific leaf blade seems to be regulated by abundance of a correctly spliced OsHKT1;4 transcript in a corresponding sheath.
The level of OsHKT1;4 transcripts was prominent in leaf sheaths throughout the growth stages.
Taken together, our results indicate a newly recognized function of OsHKT1;4 in Na(+) exclusion in stems together with leaf sheaths, thus excluding Na(+) from leaf blades of a japonica rice cultivar in the reproductive growth stage, but the contribution is low when the plants are in the vegetative growth stage.
In addition, (22)Na(+) tracer experiments using peduncles of RNAi and WT plants suggested xylem Na(+) unloading by OsHKT1;4.
Unexpectedly however, we demonstrate here accumulation of OsHKT1;4 transcripts in the stem including internode II and peduncle in the reproductive growth stage.
Moreover, phenotypic analysis of OsHKT1;4 RNAi plants in the vegetative growth stage revealed no profound influence on the growth and ion accumulation in comparison with WT plants upon salinity stress.
Here, we report results of the functional characterization of the OsHKT1;4 transporter in rice.
T-DNA Tagging-Based Gain-of-Function of OsHKT1;4 Reinforces Na Exclusion from Leaves and Stems but Triggers Na Toxicity in Roots of Rice Under Salt Stress.
Here, we have characterized a transfer DNA (T-DNA) insertion mutant line of rice, which overexpresses OsHKT1;4, owing to enhancer elements in the T-DNA, to gain an insight into the impact of OsHKT1;4 on salt tolerance of rice.
We observed that progressive desalinization of the xylem sap along its ascent to the leaf blades still occurred in plants grown at submillimolar Na+ concentration, and that OsHKT1;4 was involved in reducing xylem sap Na+ concentration in roots in these conditions too.
Constitutive Contribution by the Rice OsHKT1;4 Na + Transporter to Xylem Sap Desalinization and Low Na + Accumulation in Young Leaves Under Low as High External Na + Conditions.
By analyzing transgenic rice plants expressing a GUS reporter gene construct, we observed that OsHKT1;4 is mainly expressed in xylem parenchyma in both roots and leaves.
Using mutant lines expressing artificial microRNA that selectively reduced OsHKT1;4 expression, the involvement of OsHKT1;4 in retrieving Na+ from the xylem sap in the roots upon salt stress was evidenced.
Three rice HKT genes, OsHKT1;1, OsHKT1;4 and OsHKT1;5, are known to contribute to salt tolerance by reducing Na+ accumulation in shoots upon salt stress.
Since OsHKT1;4 was found to be also well expressed in the roots in absence of salt stress, we extended the analysis of its role when plants were subjected to non-toxic Na+ conditions (0.
Here, we further investigate the mechanisms by which OsHKT1;4 contributes to this process and extend this analysis to the role of this transporter in plants in presence of low Na+ concentrations.

Functional Keywords

Literature and News

Gene Resources

Homologs

Sequences

cDNA Sequence

>LOC_Os04g51830.1
GGAGCTCATCCACAAGCTCGCCGAGCCAGCCGTTGCACCGGCCGCGCCGGAGCGAGCACACGGCGGCCGCGTGCGTGCTCCAATATGCCCACGTCGCGGCGCGCGCTCGCCGGCGGCGCTTTGTCCATGCACGTCGCCTACTTCCTCGCCATCTCCTGCCTCGGCTACGGCCTCCTCGGGGTGCTCAAGGTGCGGGAGCCCGGCGCCGCGCCGCGCCGCATCGACCGCTTCTTCACCGCCGTGTCGGCCGCCACGGTGTCGAGCATGTCCACCGTCGAGATGGAGGTGTTCTCCAACGGCCAGCTCGTGGTGCTCACCGTCCTGATGCTCCTCGGCGGCGAGGTGTTCGTCTCGCTCGTGGGGCTCGCGTCCAAGTGGTCCAAGCTGCGGAGCGACGCCATGGACAGATCCCGGCGCGTCGAGAGCCACGGCGACGTCGCGCTCGCCGACATCGACGGCGGCGACGTCGAGAACCCGACGTCGTCGGGAGAGGAGGCGGCGAGCCGACGCCGCCCGATGGACGCGGACACGCTGCGGCACAATGCGGTGCGCGCGCTGTTCTACATCGTGCTCGCCATCTTCGCGGTGGTGCACGTCGTCGGCGCCGTGGCCGTCGCGGCGTACGTGCTCGCGTCGCCGGGCGCGAGGCGGACGCTGGGGGACAAGTCGCTGAACACGTGGACGTTCGCGGTGTTCACGACGGTGTCGACGTTCTCCAACTGCGGGTTCATGCCGACGAACGAGAACATGGTGGTGTTCAAGCGGGACGCGCCGCTGCAGCTGCTGCTGGTGCCGCAGGTGCTGGCGGGGAACACGCTGTTCGCGCCGCTGCTGGCGGCGTGCGTGTGGGCGGCGGCGGCGGCGACGCGGCGGGAGGAGCTCGTGGAGATGGCGAGGGAGGGGGGCAGGGCGGCGGCGGCCGGGTACGCGCACCTGATGCCGGCGCGGCGGTGCTGGATGCTGGCGGCGACGGTGGCGGCGTTCGTCGCCGTGCTGATGGCGCTGGTGTGCGGCATGGAGTGGGGCGGGGCGCTGCAGGGGATGAGCCCGTGGGAGAAGGTGGTGAACGCGCTGTTCCTCGCCGTGAACGCCCGGCACACCGGCGAGTCCACCGTCGACCTCTCCATCCTCGCGCCGGCCATCCTCGTGCTCTTCGTCCTCATGATGTATCTACCTCCGTACACGACGTGGTTCCCATTTGAAGAGAATTCCACTACTAAGGATAGTAATGCAGAGAACCAGGGAATCAGACTGCTCGAGAGTACACTTTTGTCACAACTCTCCTACCTGACCATCTTTGTCATTGCCATCTGCATCACCGAGAGAAGAAAGCTCAAAGAAGACCCCCTCAACTTCAGTGTGCTAAGCATTGTTGTCGAAGTTGTCAGTGCATATGGAAATGTGGGGTTCTCAATGGGCTACAGTTGCAGTAGACAGATCAATCCAGACCATCTCTGCACAGATAAATGGACTGGTTTCGTAGGGAGATGGAGCGACTCTGGCAAACTGATACTCATTTTTGTGATGTTCTTTGGGAGGCTCAAGAAGTTCAGCATGAAAGGAGGCAAAGCCTGGAAACTTAGTTAGATCTTAGATGCAGTACCAGCTGAACATTGCAGGTCCCTGAGGGTAGGAAAAAAGTATAAAGATGTACTCTTTCTCTTCCGTTTGTTTGTATATTCATTTTCACATAGACAGGAACCTTCCACTGTCATCCTTGCACAAAGCTCACCCTCCACTCCAAGTGTTGGCAGTTTCAGATCAGGTGAATGAAAGTTCATCACAAGAAATCAACATAGGCTAATTAACCTGATTCACTTCACATAGAGATGCTTCTGTCACTGGCTCCCTGCAATGACCATTTATTGCATCCATGTAATGCTAGTAAAAGGCCGGATGTCATTGTGATGGCTGCAATACTTAAATACCCACATGTTAAAAGATATGTAGATGTCTATCAAGTGGTGATCAGTTCATACAGTACGTCCAGAAGTACTTTCTTCTGAAGCAAGGGCATCTAGAAGAACCTGAGAGCTGAGATCACAAAGATTGAAAAAACACTAGATTGCTTATCCTAGTCACTACACTTTTCTGATAGAAATTAAAAAGGACACACGTGCAACCAAGGGTGCATCAGTGAAAAAAAGAGAGAATAAAAGGGAAAAGGGAAAGAAAATCTCCACTACACATTGTGCATTCTGGTACACTTGTTCCCACTACAGAAGATGTTGATTATATAGCAATAAAGCAAACAATCTAGTATTTGTGG

CDS Sequence

>LOC_Os04g51830.1
ATGCCCACGTCGCGGCGCGCGCTCGCCGGCGGCGCTTTGTCCATGCACGTCGCCTACTTCCTCGCCATCTCCTGCCTCGGCTACGGCCTCCTCGGGGTGCTCAAGGTGCGGGAGCCCGGCGCCGCGCCGCGCCGCATCGACCGCTTCTTCACCGCCGTGTCGGCCGCCACGGTGTCGAGCATGTCCACCGTCGAGATGGAGGTGTTCTCCAACGGCCAGCTCGTGGTGCTCACCGTCCTGATGCTCCTCGGCGGCGAGGTGTTCGTCTCGCTCGTGGGGCTCGCGTCCAAGTGGTCCAAGCTGCGGAGCGACGCCATGGACAGATCCCGGCGCGTCGAGAGCCACGGCGACGTCGCGCTCGCCGACATCGACGGCGGCGACGTCGAGAACCCGACGTCGTCGGGAGAGGAGGCGGCGAGCCGACGCCGCCCGATGGACGCGGACACGCTGCGGCACAATGCGGTGCGCGCGCTGTTCTACATCGTGCTCGCCATCTTCGCGGTGGTGCACGTCGTCGGCGCCGTGGCCGTCGCGGCGTACGTGCTCGCGTCGCCGGGCGCGAGGCGGACGCTGGGGGACAAGTCGCTGAACACGTGGACGTTCGCGGTGTTCACGACGGTGTCGACGTTCTCCAACTGCGGGTTCATGCCGACGAACGAGAACATGGTGGTGTTCAAGCGGGACGCGCCGCTGCAGCTGCTGCTGGTGCCGCAGGTGCTGGCGGGGAACACGCTGTTCGCGCCGCTGCTGGCGGCGTGCGTGTGGGCGGCGGCGGCGGCGACGCGGCGGGAGGAGCTCGTGGAGATGGCGAGGGAGGGGGGCAGGGCGGCGGCGGCCGGGTACGCGCACCTGATGCCGGCGCGGCGGTGCTGGATGCTGGCGGCGACGGTGGCGGCGTTCGTCGCCGTGCTGATGGCGCTGGTGTGCGGCATGGAGTGGGGCGGGGCGCTGCAGGGGATGAGCCCGTGGGAGAAGGTGGTGAACGCGCTGTTCCTCGCCGTGAACGCCCGGCACACCGGCGAGTCCACCGTCGACCTCTCCATCCTCGCGCCGGCCATCCTCGTGCTCTTCGTCCTCATGATGTATCTACCTCCGTACACGACGTGGTTCCCATTTGAAGAGAATTCCACTACTAAGGATAGTAATGCAGAGAACCAGGGAATCAGACTGCTCGAGAGTACACTTTTGTCACAACTCTCCTACCTGACCATCTTTGTCATTGCCATCTGCATCACCGAGAGAAGAAAGCTCAAAGAAGACCCCCTCAACTTCAGTGTGCTAAGCATTGTTGTCGAAGTTGTCAGTGCATATGGAAATGTGGGGTTCTCAATGGGCTACAGTTGCAGTAGACAGATCAATCCAGACCATCTCTGCACAGATAAATGGACTGGTTTCGTAGGGAGATGGAGCGACTCTGGCAAACTGATACTCATTTTTGTGATGTTCTTTGGGAGGCTCAAGAAGTTCAGCATGAAAGGAGGCAAAGCCTGGAAACTTAGTTAG

Protein Sequence

>LOC_Os04g51830.1
MPTSRRALAGGALSMHVAYFLAISCLGYGLLGVLKVREPGAAPRRIDRFFTAVSAATVSSMSTVEMEVFSNGQLVVLTVLMLLGGEVFVSLVGLASKWSKLRSDAMDRSRRVESHGDVALADIDGGDVENPTSSGEEAASRRRPMDADTLRHNAVRALFYIVLAIFAVVHVVGAVAVAAYVLASPGARRTLGDKSLNTWTFAVFTTVSTFSNCGFMPTNENMVVFKRDAPLQLLLVPQVLAGNTLFAPLLAACVWAAAAATRREELVEMAREGGRAAAAGYAHLMPARRCWMLAATVAAFVAVLMALVCGMEWGGALQGMSPWEKVVNALFLAVNARHTGESTVDLSILAPAILVLFVLMMYLPPYTTWFPFEENSTTKDSNAENQGIRLLESTLLSQLSYLTIFVIAICITERRKLKEDPLNFSVLSIVVEVVSAYGNVGFSMGYSCSRQINPDHLCTDKWTGFVGRWSDSGKLILIFVMFFGRLKKFSMKGGKAWKLS*