Information report for MPR25
Gene Details
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Functional Descriptions
- In this study, we investigated the molecular function in rice of the mitochondrial PPR-encoding gene MITOCHONDRIAL PPR25 (MPR25), which belongs to the E subgroup of the PPR family.
- FLAG-tagged MPR25 accumulated in mitochondria but not in chloroplasts.
- Direct sequencing revealed that the MPR25 mutant fails to edit a C-U RNA editing site at nucleotide 1580 of nad5, which encodes a subunit of complex I (NADH dehydrogenase) of the respiratory chain in mitochondria.
- The MPR25 mutant may therefore provide new information on the coordinated interaction between mitochondria and chloroplasts.
- Rice MPR25 encodes a pentatricopeptide repeat protein and is essential for RNA editing of nad5 transcripts in mitochondria.
- A Tos17 knockout mutant of MPR25 exhibited growth retardation and pale-green leaves with reduced chlorophyll content during the early stages of plant development.
- We also show that splicing of chloroplast atpF transcripts is affected in the rice MPR25 mutant.
Functional Keywords
Literature and News
- Rice MPR25 encodes a pentatricopeptide repeat protein and is essential for RNA editing of nad5 transcripts in mitochondria . DOI: 10.1111/j.1365-313X.2012.05091.x ; PMID: 22747551
- AEF1/MPR25 is implicated in RNA editing of plastid atpF and mitochondrial nad5, and also promotes atpF splicing in Arabidopsis and rice . DOI: 10.1111/tpj.12756 ; PMID: 25585673
Gene Resources
- UniProt: A0A0N7KJM8
- EMBL: AL606638, AP008210
- AlphaFoldDB: A0A0N7KJM8
- EnsemblPlants: Os04t0602600-01
- Gramene: Os04t0602600-01
- KEGG: dosa:Os04g0602600, osa:4336891
- Orthologous matrix: CCIADML
- InterPro: IPR002885, IPR011990, IPR046848
- PANTHER: PTHR47926, PTHR47926:SF452
- SUPFAM: SSF48452
- PROSITE: PS51375
- Gene3D: 1.25.40.10
- OrthoDB: A0A0N7KJM8
- SWISS-MODEL: A0A0N7KJM8
Homologs
- Arabidopsis thaliana: AT3G22150
- Gossypium barbadense: Gobar.A02G135500, Gobar.D03G069200
- Gossypium hirsutum: Gohir.A02G124200
- Lactuca sativa: Lsat_1_v5_gn_3_119881
- Rosa chinensis: RchiOBHm_Chr5g0011421
Sequences
cDNA Sequence
- >LOC_Os04g51350.1
ATCGAATCGTCTGTGGCGCCCGCGAAGCAACGGAAGAGATGAGGAGATGACTCGGATGAGCGGAAGCTGCAGGGAGCGAGTCGTCGTCGTCGTCGTCACCGCGAGGCGCCGCGCCAACCTCTCATGTCCTCTCCTCGCTGCGCCGTCTCGCTCCCACCCACCGCCGCGGCGACCACCGCCACCAATGGCGGAGGCGGCGGGAGGAGGAACGCGCAGCCGGCAGCGGCGACGGCGGCGTCGCAGGTGAAGAAGCTGTGCAAGCAGGGGCGGCTGGACCACGCGCGGCGGCTGCTCCTCGAGGCGCTTCCCCGGCCGCCACCGACGCTGCTCTGCAACGCGCTCCTCATCGCCTACGCCGCCCGCGCGCTCCCGGAGGAGGCGCTCCGCCTCTACGCTCTCCTCAACCACGCCGCGCGCCCGCCGGTCCGCTCCGACCACTACACCTACTCCGCCGCGCTCACCGCCTGCGCACGCTCCCGCCGCCTCCGCCTCGGGAGGTCCGTCCACGCGCACATGCTCCGGCGCGCCCGCTCCCTCCCGGACACCGCCGTCCTCCGCAACTCCCTCCTCAACCTCTACGCCTCCAGCGTGCGGTACCGGGAGGCACGCGTCGACGTCGTCCGGAGGCTGTTCGACGCAATGCCTAAGAGGAACGTGGTTTCTTGGAACACTCTGTTCGGCTGGTACGTCAAGACCGGGCGTCCCCAGGAAGCCCTGGAGCTGTTTGTGCGCATGCTAGAAGATGGTTTCAGGCCCACGCCGGTCAGCTTCGTGAATATATTTCCGGCTGCTGTGGCTGACGATCCAAGCTGGCCATTCCAGCTCTACGGATTACTTGTGAAGTATGGGGTAGAGTATATCAACGATCTGTTTGTTGTGAGCTCAGCAATCGACATGTTCTCCGAATTTGGGGATGTGCAATCAGCTCGGAGGGTGTTTGATCGTGCTGCCAAGAAGAACACCGAGGTTTGGAACACCATGATCACTGGGTATGTACAGAATGGCCAGTTTTCTGAAGCCATTGATCTCTTCAGCAAAATCTTGGGATCCCGAGAGGTTCCATTGGATGTTGTGACCTTCTTATCGGCCCTCACAGCTGCCTCACAGTCACAGGATGTTAGCTTGGGTCAGCAGCTGCATGGCTATTTGATTAAAGGAATGCATAGGACATTGCCTGTAATACTCGGTAATGCACTTGTTGTGATGTACTCAAGATGTGGCAATGTCCAAACTGCCTTTGATCTCTTCGACCGGTTGCCAGAGAAGGACATTGTTACTTGGAATACCATGGTGACTGCTTTTATACAGAACGATTTTGACTTGGAAGGCTTGCTGCTCGTCTATGAGATGCAGAAATCAGGTTTTGCTGCTGATTCTGTGACATTGACTGCAGTTCTGTCAGCATCATCAAATACTGGAGACCTTCAGATCGGTAAACAAGCACATGGTTATCTTATCAGGCATGGTATCGAGGGTGAGGGCTTGGAGAGCTACCTAATAGACATGTATGCAAAATCTGGCCGTGTAGAAATGGCTCAGAGAGTGTTTGATAGCTTTAAAAATGCCAAGAGGGATGAAGTCACTTGGAATGCCATGATAGCGGGATACACACAGAGTGGACAGCCTGAAAAGGCAATCTTAGTATTCCGGGCAATGCTTGAGGCAGGTCTTGAACCTACTTCGGTGACACTTGCTTCGGTGCTACCTGCATGTGATCCTGTTGGAGGGGGCGTTTATTCTGGGAAGCAAATCCACTGTTTTGCTGTGCGCCGTTGTTTGGATACCAATGTCTTCGTAGGTACAGCTCTTATTGACATGTACTCTAAGTGTGGTGAGATCACTACAGCAGAAAATGTCTTTGGTGGCATGACGGGGAAGAGCACTGTCACCTATACGACAATGATATCTGGTCTTGGTCAGCATGGTTTCGGCAAAAAAGCGCTTGCTCTTTTCAACTCCATGCAAGAAAAGGGGTTGAAGCCTGATGCAGTGACCTTCTTGTCTGCGATTTCAGCATGTAATTACTCTGGACTCGTCGACGAAGGACTAGCTTTGTACAGGTCGATGGACTCATTTGGAATTTCGGCTACTCCTCAGCACCACTGCTGTGTTGCAGACTTGTTAGCTAAAGCTGGAAGGGTGGAGGAAGCATACGAGTTTATAGAGGGGCTGGGGGAGGAGGGCAACTTTGTTGCCATCTGGGGATCGCTGCTTGCATCCTGCAAAGCTCAGGGCAAGCAAGAGTTGGCAAAGTTGGTGACCAAGAAGCTGCTTGACATCGAGAAGCAGTACGGTCATGCAGGCTACAGCGTTTTGTTGTCACAGGTCCTTGCTGCTGAAAGTAACTGGAATAGTGCTGATAGTCTAAGAAAGGAGATGAGGGCAAGGGGATTGAAGAAAGAAGCAGGTTCTAGTTGGATTAAAGTCCAGAACGCAGCATTGGAACACAAGTTTATCGAAAAAGACCAAAATTATGTCGAAAATGAGCACATGTTCTCGATTCTGGATGGTGATGCCGACAGTACGGATAGACTTTAACATGTTTCAATACCTTTTGGCACGCAAGATTGCAGATATGAGATTCCTAATCCTATTGTGGTGATTACCTCACGCTGACAGACTAGGCTCCTGTTTTACCTGCAGTTTAACATGATTTATCTTCATTCAGAAATCAGAATTGATGCTCCTCATAGTAATACTTGACTGGATGGAAACTTGGAAAGACACCTAGCCCACAAACCTCATGCGGCGGCCACACGGTGAACTTGTGCTGCGAGACATCAATCTATGGATGAGGTTGATACCAATGATGTGCCAGATGAAGAGCAAGCTGCAATCTTGCAGGTGGCCAGGTTGTAACTTGTATTCAGTAGTACGCATATCTTGACAAGCACAGCGCCCTCCTTAGGGATTCTGGGTGAATTGTACAGCTACATTTGCTTATAATCGTCTAGAAATAGGTGAGTACTGTCATAGAACGAGCAACGTGATTAGGCAACGGGATTTTTTTTGGTTGTGAACGTACATACAATATCTTAAATGAAGATTATTCTCACATGATTACGGCCAATGCAAAGGAGGATTTTGCATTGGTTTTCTGT
CDS Sequence
- >LOC_Os04g51350.1
ATGTCCTCTCCTCGCTGCGCCGTCTCGCTCCCACCCACCGCCGCGGCGACCACCGCCACCAATGGCGGAGGCGGCGGGAGGAGGAACGCGCAGCCGGCAGCGGCGACGGCGGCGTCGCAGGTGAAGAAGCTGTGCAAGCAGGGGCGGCTGGACCACGCGCGGCGGCTGCTCCTCGAGGCGCTTCCCCGGCCGCCACCGACGCTGCTCTGCAACGCGCTCCTCATCGCCTACGCCGCCCGCGCGCTCCCGGAGGAGGCGCTCCGCCTCTACGCTCTCCTCAACCACGCCGCGCGCCCGCCGGTCCGCTCCGACCACTACACCTACTCCGCCGCGCTCACCGCCTGCGCACGCTCCCGCCGCCTCCGCCTCGGGAGGTCCGTCCACGCGCACATGCTCCGGCGCGCCCGCTCCCTCCCGGACACCGCCGTCCTCCGCAACTCCCTCCTCAACCTCTACGCCTCCAGCGTGCGGTACCGGGAGGCACGCGTCGACGTCGTCCGGAGGCTGTTCGACGCAATGCCTAAGAGGAACGTGGTTTCTTGGAACACTCTGTTCGGCTGGTACGTCAAGACCGGGCGTCCCCAGGAAGCCCTGGAGCTGTTTGTGCGCATGCTAGAAGATGGTTTCAGGCCCACGCCGGTCAGCTTCGTGAATATATTTCCGGCTGCTGTGGCTGACGATCCAAGCTGGCCATTCCAGCTCTACGGATTACTTGTGAAGTATGGGGTAGAGTATATCAACGATCTGTTTGTTGTGAGCTCAGCAATCGACATGTTCTCCGAATTTGGGGATGTGCAATCAGCTCGGAGGGTGTTTGATCGTGCTGCCAAGAAGAACACCGAGGTTTGGAACACCATGATCACTGGGTATGTACAGAATGGCCAGTTTTCTGAAGCCATTGATCTCTTCAGCAAAATCTTGGGATCCCGAGAGGTTCCATTGGATGTTGTGACCTTCTTATCGGCCCTCACAGCTGCCTCACAGTCACAGGATGTTAGCTTGGGTCAGCAGCTGCATGGCTATTTGATTAAAGGAATGCATAGGACATTGCCTGTAATACTCGGTAATGCACTTGTTGTGATGTACTCAAGATGTGGCAATGTCCAAACTGCCTTTGATCTCTTCGACCGGTTGCCAGAGAAGGACATTGTTACTTGGAATACCATGGTGACTGCTTTTATACAGAACGATTTTGACTTGGAAGGCTTGCTGCTCGTCTATGAGATGCAGAAATCAGGTTTTGCTGCTGATTCTGTGACATTGACTGCAGTTCTGTCAGCATCATCAAATACTGGAGACCTTCAGATCGGTAAACAAGCACATGGTTATCTTATCAGGCATGGTATCGAGGGTGAGGGCTTGGAGAGCTACCTAATAGACATGTATGCAAAATCTGGCCGTGTAGAAATGGCTCAGAGAGTGTTTGATAGCTTTAAAAATGCCAAGAGGGATGAAGTCACTTGGAATGCCATGATAGCGGGATACACACAGAGTGGACAGCCTGAAAAGGCAATCTTAGTATTCCGGGCAATGCTTGAGGCAGGTCTTGAACCTACTTCGGTGACACTTGCTTCGGTGCTACCTGCATGTGATCCTGTTGGAGGGGGCGTTTATTCTGGGAAGCAAATCCACTGTTTTGCTGTGCGCCGTTGTTTGGATACCAATGTCTTCGTAGGTACAGCTCTTATTGACATGTACTCTAAGTGTGGTGAGATCACTACAGCAGAAAATGTCTTTGGTGGCATGACGGGGAAGAGCACTGTCACCTATACGACAATGATATCTGGTCTTGGTCAGCATGGTTTCGGCAAAAAAGCGCTTGCTCTTTTCAACTCCATGCAAGAAAAGGGGTTGAAGCCTGATGCAGTGACCTTCTTGTCTGCGATTTCAGCATGTAATTACTCTGGACTCGTCGACGAAGGACTAGCTTTGTACAGGTCGATGGACTCATTTGGAATTTCGGCTACTCCTCAGCACCACTGCTGTGTTGCAGACTTGTTAGCTAAAGCTGGAAGGGTGGAGGAAGCATACGAGTTTATAGAGGGGCTGGGGGAGGAGGGCAACTTTGTTGCCATCTGGGGATCGCTGCTTGCATCCTGCAAAGCTCAGGGCAAGCAAGAGTTGGCAAAGTTGGTGACCAAGAAGCTGCTTGACATCGAGAAGCAGTACGGTCATGCAGGCTACAGCGTTTTGTTGTCACAGGTCCTTGCTGCTGAAAGTAACTGGAATAGTGCTGATAGTCTAAGAAAGGAGATGAGGGCAAGGGGATTGAAGAAAGAAGCAGGTTCTAGTTGGATTAAAGTCCAGAACGCAGCATTGGAACACAAGTTTATCGAAAAAGACCAAAATTATGTCGAAAATGAGCACATGTTCTCGATTCTGGATGGTGATGCCGACAGTACGGATAGACTTTAA
Protein Sequence
- >LOC_Os04g51350.1
MSSPRCAVSLPPTAAATTATNGGGGGRRNAQPAAATAASQVKKLCKQGRLDHARRLLLEALPRPPPTLLCNALLIAYAARALPEEALRLYALLNHAARPPVRSDHYTYSAALTACARSRRLRLGRSVHAHMLRRARSLPDTAVLRNSLLNLYASSVRYREARVDVVRRLFDAMPKRNVVSWNTLFGWYVKTGRPQEALELFVRMLEDGFRPTPVSFVNIFPAAVADDPSWPFQLYGLLVKYGVEYINDLFVVSSAIDMFSEFGDVQSARRVFDRAAKKNTEVWNTMITGYVQNGQFSEAIDLFSKILGSREVPLDVVTFLSALTAASQSQDVSLGQQLHGYLIKGMHRTLPVILGNALVVMYSRCGNVQTAFDLFDRLPEKDIVTWNTMVTAFIQNDFDLEGLLLVYEMQKSGFAADSVTLTAVLSASSNTGDLQIGKQAHGYLIRHGIEGEGLESYLIDMYAKSGRVEMAQRVFDSFKNAKRDEVTWNAMIAGYTQSGQPEKAILVFRAMLEAGLEPTSVTLASVLPACDPVGGGVYSGKQIHCFAVRRCLDTNVFVGTALIDMYSKCGEITTAENVFGGMTGKSTVTYTTMISGLGQHGFGKKALALFNSMQEKGLKPDAVTFLSAISACNYSGLVDEGLALYRSMDSFGISATPQHHCCVADLLAKAGRVEEAYEFIEGLGEEGNFVAIWGSLLASCKAQGKQELAKLVTKKLLDIEKQYGHAGYSVLLSQVLAAESNWNSADSLRKEMRARGLKKEAGSSWIKVQNAALEHKFIEKDQNYVENEHMFSILDGDADSTDRL*