Information report for OsSPX-MFS1;OsPSS1
Gene Details
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Functional Descriptions
- Here we report on the characterization of rice osa-miR827 and its two target genes, OsSPX-MFS1 and OsSPX-MFS2, which encode SPX-MFS proteins predicted to be implicated in phosphate (Pi) sensing or transport.
- OsSPX-MFS1 mRNA is mainly expressed in shoots under sufficient Pi supply while its expression is reduced on Pi starvation, revealing a direct relationship between induction of osa-miR827 and down-regulation of OsSPX-MFS1.
- Functional characterization of the rice SPX-MFS family reveals a key role of OsSPX-MFS1 in controlling phosphate homeostasis in leaves.
- The function of OsSPX-MFS1 in Pi homeostasis was analysed using an OsSPX-MFS1 mutant (mfs1) and osa-miR827 overexpression line (miR827-Oe).
- Mutation in OsSPX-MFS1 (mfs1) and overexpression of the upstream miR827 (miR827-Oe) plants impaired Pi homeostasis in the leaves.
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- The results suggest that OsSPX-MFS1 is a key player in maintaining Pi homeostasis in the leaves, potentially acting as a Pi transporter.
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- Transcript analyses revealed that members of the SPX-MFS family were mainly expressed in the shoots, with OsSPX-MFS1 and OsSPX-MFS3 being suppressed by Pi deficiency, while OsSPX-MFS2 was induced.
- Finally, heterologous complementation of a yeast mutant impaired in Pi transporter was used to assess the capacity of OsSPX-MFS1 to transport Pi.
- In addition, studies in yeast revealed that OsSPX-MFS1 may be involved in Pi transport.
- In parallel, we analyzed the responses of the two OsSPX-MFS1 and OsSPX-MFS2 gene targets to Pi starvation.
- This suggests that OsSPX-MFS1 and OsSPX-MFS2 are both negatively regulated by osa-miR827 abundance although they respond differently to external Pi conditions.
- Furthermore, we show that heterologous expression of the rice homologue OsSPX-MFS1 mediates Pi influx to yeast vacuoles.
Functional Keywords
- phosphate , shoot , homeostasis , transporter , pi , Pi
Literature and News
- Auxin response factor (OsARF12), a novel regulator for phosphate homeostasis in rice (Oryza sativa) . DOI: 10.1111/nph.12499 ; PMID: 24111723
- Functional characterization of the rice SPX-MFS family reveals a key role of OsSPX-MFS1 in controlling phosphate homeostasis in leaves . DOI: 10.1111/j.1469-8137.2012.04227.x ; PMID: 22803610
- Complex regulation of two target genes encoding SPX-MFS proteins by rice miR827 in response to phosphate starvation . DOI: 10.1093/pcp/pcq170 ; PMID: 21062869
- Identification of plant vacuolar transporters mediating phosphate storage . DOI: 10.1038/ncomms11095 ; PMID: 27029856
Gene Resources
- UniProt: Q0JAW2
- EMBL: AK066068, AL606640, AP008210
- AlphaFoldDB: Q0JAW2
- EnsemblPlants: Os04t0573000-01
- Gramene: Os04t0573000-01
- KEGG: dosa:Os04g0573000, osa:4336724
- Orthologous matrix: WLMAIAW
- InterPro: IPR004331, IPR011701, IPR036259
- PANTHER: PTHR23510, PTHR23510:SF72
- SUPFAM: SSF103473
- PROSITE: PS51382
- Gene3D: 1.20.1250.20
- OrthoDB: Q0JAW2
- Conserved Domain Database: cd14479
- eggNOG: KOG1161, KOG2325
Sequences
cDNA Sequence
- >LOC_Os04g48390.1
ACCCCTCCCTCAGCTTCCCTTCCCATCTGCCCGTCTTCTTCTTCTTCCTCCTCCTCCTGCCCTACCCGCGGCCGCGCGATATAAATACATCACCCTCCCCATCCGCCTCTCCGCCAGCAAACCAGCACAGCCGTACGCCGCCGGCAACTGCGTTCGCGCGAGCGCCTGTCCGCGTTGTACATATTTGTGAGTAATCCGTCACCTCTGTACGCAGCACGCACATGCCGCCGCCAAAGCGCAGATGATTCCAGTCTTTCCCTACTTTTGAATCTGTGCCTGATGAATCTGCGTCGTCGTGCTGTAGCACTAGCGAGTGTTTCCCTTGCGACGTAGAGTTTGCTGATGTTCATCTAATTAGCCCGGAGGACTCGGGAATCAGAATTCACCGTGGTGTCAGGGAGAAAAAGTGGATCGAACTTCGGATCACCTGGTACCATTTGTTTACGATACAGGTATTGAACGACAAGATGGTTAATTTTGGAAAGAAGTTGATGGCTGATCAAGTAGAAGAATGGAAAGGATACTATATTAACTACAAATTGATGAAGAAAATGTTGAAGCAATACGTTCAGCAGACCCAACTTGGTGGAAAAGATCGAGAGCAAGTACTTAAAGAGTTCTCAAGAATTCTTGATGAACAGATTGAAAGGATTGTACTTTTTCTGCTACAACAACAAGGCCACCTTGCTAATAGGATTGAGGAATTGGGAGAACAGCGTGCTGCTCTTCTAGAACAGCATGATATATCCCAAGTTTTTCAACTACGAGAGGCTTATAGAGAAGTTGGGCGAGATCTTATAAAGCTTCTCCGTTTTGTTGATATGAATGCTACTGGTATAAGGAAGATTCTAAAGAAATTCGATAAGCGCTTTGGCTATAGGTTTACAGACTATTATGTCACCACTCGTGCAAACCATCCTTATTCTCAGCTTCAACAAGTCTTTAAGCAAGTGGGGATTGTAGCTGTTGTAGGAGCTTTATCGCGCAACCTTGCATATCTGCAAGATCATGAAGGAAGTGTTTTGTCCATCTATGATCATCCATCAGTTACCTTAAAGGACCCTATCATAGACCAAGTAAATCATGCGGTACAAAAACTTACACATGCGACGAGTTTTCTGCAATTCTTAGGTCAACATGCACTTATCATCCAAGAGGACGTGCAAAGTGGGTCGGAGGATCTTGTCGATGATCAAAGTTACCATTTCATGTCTTTGATACTCAACCTAGTGAACACCTTTCTTTACATGGTGAACACATATATCATTGTGCCAACTGCTGATGACTACGCTGTAAGCCTTGGAGCTGCAGCGACAGTTTGTGGCGTAATCATCGGATCAATGGCAGTTGCTCAAGTGTTCTCCTCAGTTTATTTCAGTGCATGGTCAAATAGGTCATACTTCAGACCTCTTGTATTCAGTAGCATCATGTTATTCGCTGGGAATCTGCTATATGCATTAGCATATGACCTGAATTCTCTGACAGTTCTCCTGATTGGCCGGCTACTATGCGGGTTAGGCTCTGCAAGAGCTGTGAACCGTCGATATATCAGCGATTGTGTGCCTCTCAAAATCAGGCTACAGGCCTCTGCAGGATTCGTCAGTGCTAGTGCTCTTGGCATGGCATGTGGCCCTGCTCTTGCTGGTTTACTCCAGACAAGATTTAAGATATACTCACTCACTTTTGATCAGAGCACGTTGCCTGGGTGGGTCATGTGCATTGCTTGGCTTGTTTACTTGCTGTGGCTGTGGATATCATTCAAAGAGCCGGGACACTTTGCTAAGAGTTCAGACACGGCACAGCCTGCTGAATCTGGCCATCAAGTCAATGCTAATCTGGAGGAAGGTCTAGCGCAACCATTGCTTACAGGCTCAGAAGAGGGACAAGACCAAAATGCTGAGGATAATGACGATAACGAAGAGGAGTCTAAAAATTCTCACGGGCCAGCAACTTCAATCAGTTCAGCATACAAATTGTTGACACCATCTGTGAAGGTCCAGCTATTGATATACTTTATGCTCAAGTACGCTATGGAAATCTTGCTCTCTGAATCAAGTGTCATCACCACATACTATTTTAACTGGTCTACAAGCGCTGTGGCTATCTTTTTAGCGATTCTTGGGTGTACTGTTCTCCCTGTCAACGCCATTGTTGGAAGCTACATTACAAATTTATTCGAGGACAGGCAAATTTTGGTGGCATCTGAAATCATGGTCCTCATTGGTATAATCATGAGCTTCCGTTACACCCCTCACTACTCCGTTCCGCAGTACGTCTTGTCCGCTCTCATCACATTTGTGTTTGCTGAGGTCCTCGAAGGAGTGAACCTGTCGTTGCTTTCACGAGTAATGTCGTCGAGGCTCGCGCGAGGAACCTACAACGGCGGCCTCCTGTCGACAGAGGCCGGGACGCTGGCCCGTGTAGTTGCAGACGCGACGATTACCGCGGCGGGTTATCTAGGCCCGGACCTCCTGCTCAACATCACTCTGCTGCCACCTCTTGTCATCTGCATAGCCTCCCTCGTTGCAACATTCTGCACATACAACACCCTGTACTAAGGAAGTTGCCTCTCTCTGCACACTGTAGAGCCGGTTAAACTTTATTCATGCTCACATTGGGTTGTATTTTACATGTATCTGCATGCGGTAGCATTCTTTGTTTGTACAAGTCCTTAAACAGTTCAATAAAATGTTCTTCTGTAAATCCTACAGATGTTTTCTCGATGCATGTTTACAGTCCTTATGCATTTTACATATGTATTCATTTTTCCTATATCTGTAAAAAAAATTTCTTGATCTGTACCAATATTATGAGTTATGAGGTTGAAAAGACACATGCACCATGGAC
CDS Sequence
- >LOC_Os04g48390.1
ATGGTTAATTTTGGAAAGAAGTTGATGGCTGATCAAGTAGAAGAATGGAAAGGATACTATATTAACTACAAATTGATGAAGAAAATGTTGAAGCAATACGTTCAGCAGACCCAACTTGGTGGAAAAGATCGAGAGCAAGTACTTAAAGAGTTCTCAAGAATTCTTGATGAACAGATTGAAAGGATTGTACTTTTTCTGCTACAACAACAAGGCCACCTTGCTAATAGGATTGAGGAATTGGGAGAACAGCGTGCTGCTCTTCTAGAACAGCATGATATATCCCAAGTTTTTCAACTACGAGAGGCTTATAGAGAAGTTGGGCGAGATCTTATAAAGCTTCTCCGTTTTGTTGATATGAATGCTACTGGTATAAGGAAGATTCTAAAGAAATTCGATAAGCGCTTTGGCTATAGGTTTACAGACTATTATGTCACCACTCGTGCAAACCATCCTTATTCTCAGCTTCAACAAGTCTTTAAGCAAGTGGGGATTGTAGCTGTTGTAGGAGCTTTATCGCGCAACCTTGCATATCTGCAAGATCATGAAGGAAGTGTTTTGTCCATCTATGATCATCCATCAGTTACCTTAAAGGACCCTATCATAGACCAAGTAAATCATGCGGTACAAAAACTTACACATGCGACGAGTTTTCTGCAATTCTTAGGTCAACATGCACTTATCATCCAAGAGGACGTGCAAAGTGGGTCGGAGGATCTTGTCGATGATCAAAGTTACCATTTCATGTCTTTGATACTCAACCTAGTGAACACCTTTCTTTACATGGTGAACACATATATCATTGTGCCAACTGCTGATGACTACGCTGTAAGCCTTGGAGCTGCAGCGACAGTTTGTGGCGTAATCATCGGATCAATGGCAGTTGCTCAAGTGTTCTCCTCAGTTTATTTCAGTGCATGGTCAAATAGGTCATACTTCAGACCTCTTGTATTCAGTAGCATCATGTTATTCGCTGGGAATCTGCTATATGCATTAGCATATGACCTGAATTCTCTGACAGTTCTCCTGATTGGCCGGCTACTATGCGGGTTAGGCTCTGCAAGAGCTGTGAACCGTCGATATATCAGCGATTGTGTGCCTCTCAAAATCAGGCTACAGGCCTCTGCAGGATTCGTCAGTGCTAGTGCTCTTGGCATGGCATGTGGCCCTGCTCTTGCTGGTTTACTCCAGACAAGATTTAAGATATACTCACTCACTTTTGATCAGAGCACGTTGCCTGGGTGGGTCATGTGCATTGCTTGGCTTGTTTACTTGCTGTGGCTGTGGATATCATTCAAAGAGCCGGGACACTTTGCTAAGAGTTCAGACACGGCACAGCCTGCTGAATCTGGCCATCAAGTCAATGCTAATCTGGAGGAAGGTCTAGCGCAACCATTGCTTACAGGCTCAGAAGAGGGACAAGACCAAAATGCTGAGGATAATGACGATAACGAAGAGGAGTCTAAAAATTCTCACGGGCCAGCAACTTCAATCAGTTCAGCATACAAATTGTTGACACCATCTGTGAAGGTCCAGCTATTGATATACTTTATGCTCAAGTACGCTATGGAAATCTTGCTCTCTGAATCAAGTGTCATCACCACATACTATTTTAACTGGTCTACAAGCGCTGTGGCTATCTTTTTAGCGATTCTTGGGTGTACTGTTCTCCCTGTCAACGCCATTGTTGGAAGCTACATTACAAATTTATTCGAGGACAGGCAAATTTTGGTGGCATCTGAAATCATGGTCCTCATTGGTATAATCATGAGCTTCCGTTACACCCCTCACTACTCCGTTCCGCAGTACGTCTTGTCCGCTCTCATCACATTTGTGTTTGCTGAGGTCCTCGAAGGAGTGAACCTGTCGTTGCTTTCACGAGTAATGTCGTCGAGGCTCGCGCGAGGAACCTACAACGGCGGCCTCCTGTCGACAGAGGCCGGGACGCTGGCCCGTGTAGTTGCAGACGCGACGATTACCGCGGCGGGTTATCTAGGCCCGGACCTCCTGCTCAACATCACTCTGCTGCCACCTCTTGTCATCTGCATAGCCTCCCTCGTTGCAACATTCTGCACATACAACACCCTGTACTAA
Protein Sequence
- >LOC_Os04g48390.1
MVNFGKKLMADQVEEWKGYYINYKLMKKMLKQYVQQTQLGGKDREQVLKEFSRILDEQIERIVLFLLQQQGHLANRIEELGEQRAALLEQHDISQVFQLREAYREVGRDLIKLLRFVDMNATGIRKILKKFDKRFGYRFTDYYVTTRANHPYSQLQQVFKQVGIVAVVGALSRNLAYLQDHEGSVLSIYDHPSVTLKDPIIDQVNHAVQKLTHATSFLQFLGQHALIIQEDVQSGSEDLVDDQSYHFMSLILNLVNTFLYMVNTYIIVPTADDYAVSLGAAATVCGVIIGSMAVAQVFSSVYFSAWSNRSYFRPLVFSSIMLFAGNLLYALAYDLNSLTVLLIGRLLCGLGSARAVNRRYISDCVPLKIRLQASAGFVSASALGMACGPALAGLLQTRFKIYSLTFDQSTLPGWVMCIAWLVYLLWLWISFKEPGHFAKSSDTAQPAESGHQVNANLEEGLAQPLLTGSEEGQDQNAEDNDDNEEESKNSHGPATSISSAYKLLTPSVKVQLLIYFMLKYAMEILLSESSVITTYYFNWSTSAVAIFLAILGCTVLPVNAIVGSYITNLFEDRQILVASEIMVLIGIIMSFRYTPHYSVPQYVLSALITFVFAEVLEGVNLSLLSRVMSSRLARGTYNGGLLSTEAGTLARVVADATITAAGYLGPDLLLNITLLPPLVICIASLVATFCTYNTLY*