Gene Details:

Functional Descriptions:

  • Here we report on the characterization of rice osa-miR827 and its two target genes, OsSPX-MFS1 and OsSPX-MFS2, which encode SPX-MFS proteins predicted to be implicated in phosphate (Pi) sensing or transport.
  • OsSPX-MFS1 mRNA is mainly expressed in shoots under sufficient Pi supply while its expression is reduced on Pi starvation, revealing a direct relationship between induction of osa-miR827 and down-regulation of OsSPX-MFS1.
  • Functional characterization of the rice SPX-MFS family reveals a key role of OsSPX-MFS1 in controlling phosphate homeostasis in leaves.
  • The function of OsSPX-MFS1 in Pi homeostasis was analysed using an OsSPX-MFS1 mutant (mfs1) and osa-miR827 overexpression line (miR827-Oe).
  • Mutation in OsSPX-MFS1 (mfs1) and overexpression of the upstream miR827 (miR827-Oe) plants impaired Pi homeostasis in the leaves.
    • The results suggest that OsSPX-MFS1 is a key player in maintaining Pi homeostasis in the leaves, potentially acting as a Pi transporter.
    • Transcript analyses revealed that members of the SPX-MFS family were mainly expressed in the shoots, with OsSPX-MFS1 and OsSPX-MFS3 being suppressed by Pi deficiency, while OsSPX-MFS2 was induced.
  • Finally, heterologous complementation of a yeast mutant impaired in Pi transporter was used to assess the capacity of OsSPX-MFS1 to transport Pi.
  • In addition, studies in yeast revealed that OsSPX-MFS1 may be involved in Pi transport.
  • In parallel, we analyzed the responses of the two OsSPX-MFS1 and OsSPX-MFS2 gene targets to Pi starvation.
  • This suggests that OsSPX-MFS1 and OsSPX-MFS2 are both negatively regulated by osa-miR827 abundance although they respond differently to external Pi conditions.
  • Furthermore, we show that heterologous expression of the rice homologue OsSPX-MFS1 mediates Pi influx to yeast vacuoles.

Literature:

Gene Resources:

Sequences:

cDNA Sequence
  • >LOC_Os04g48390.1
    ACCCCTCCCTCAGCTTCCCTTCCCATCTGCCCGTCTTCTTCTTCTTCCTCCTCCTCCTGCCCTACCCGCGGCCGCGCGATATAAATACATCACCCTCCCCATCCGCCTCTCCGCCAGCAAACCAGCACAGCCGTACGCCGCCGGCAACTGCGTTCGCGCGAGCGCCTGTCCGCGTTGTACATATTTGTGAGTAATCCGTCACCTCTGTACGCAGCACGCACATGCCGCCGCCAAAGCGCAGATGATTCCAGTCTTTCCCTACTTTTGAATCTGTGCCTGATGAATCTGCGTCGTCGTGCTGTAGCACTAGCGAGTGTTTCCCTTGCGACGTAGAGTTTGCTGATGTTCATCTAATTAGCCCGGAGGACTCGGGAATCAGAATTCACCGTGGTGTCAGGGAGAAAAAGTGGATCGAACTTCGGATCACCTGGTACCATTTGTTTACGATACAGGTATTGAACGACAAGATGGTTAATTTTGGAAAGAAGTTGATGGCTGATCAAGTAGAAGAATGGAAAGGATACTATATTAACTACAAATTGATGAAGAAAATGTTGAAGCAATACGTTCAGCAGACCCAACTTGGTGGAAAAGATCGAGAGCAAGTACTTAAAGAGTTCTCAAGAATTCTTGATGAACAGATTGAAAGGATTGTACTTTTTCTGCTACAACAACAAGGCCACCTTGCTAATAGGATTGAGGAATTGGGAGAACAGCGTGCTGCTCTTCTAGAACAGCATGATATATCCCAAGTTTTTCAACTACGAGAGGCTTATAGAGAAGTTGGGCGAGATCTTATAAAGCTTCTCCGTTTTGTTGATATGAATGCTACTGGTATAAGGAAGATTCTAAAGAAATTCGATAAGCGCTTTGGCTATAGGTTTACAGACTATTATGTCACCACTCGTGCAAACCATCCTTATTCTCAGCTTCAACAAGTCTTTAAGCAAGTGGGGATTGTAGCTGTTGTAGGAGCTTTATCGCGCAACCTTGCATATCTGCAAGATCATGAAGGAAGTGTTTTGTCCATCTATGATCATCCATCAGTTACCTTAAAGGACCCTATCATAGACCAAGTAAATCATGCGGTACAAAAACTTACACATGCGACGAGTTTTCTGCAATTCTTAGGTCAACATGCACTTATCATCCAAGAGGACGTGCAAAGTGGGTCGGAGGATCTTGTCGATGATCAAAGTTACCATTTCATGTCTTTGATACTCAACCTAGTGAACACCTTTCTTTACATGGTGAACACATATATCATTGTGCCAACTGCTGATGACTACGCTGTAAGCCTTGGAGCTGCAGCGACAGTTTGTGGCGTAATCATCGGATCAATGGCAGTTGCTCAAGTGTTCTCCTCAGTTTATTTCAGTGCATGGTCAAATAGGTCATACTTCAGACCTCTTGTATTCAGTAGCATCATGTTATTCGCTGGGAATCTGCTATATGCATTAGCATATGACCTGAATTCTCTGACAGTTCTCCTGATTGGCCGGCTACTATGCGGGTTAGGCTCTGCAAGAGCTGTGAACCGTCGATATATCAGCGATTGTGTGCCTCTCAAAATCAGGCTACAGGCCTCTGCAGGATTCGTCAGTGCTAGTGCTCTTGGCATGGCATGTGGCCCTGCTCTTGCTGGTTTACTCCAGACAAGATTTAAGATATACTCACTCACTTTTGATCAGAGCACGTTGCCTGGGTGGGTCATGTGCATTGCTTGGCTTGTTTACTTGCTGTGGCTGTGGATATCATTCAAAGAGCCGGGACACTTTGCTAAGAGTTCAGACACGGCACAGCCTGCTGAATCTGGCCATCAAGTCAATGCTAATCTGGAGGAAGGTCTAGCGCAACCATTGCTTACAGGCTCAGAAGAGGGACAAGACCAAAATGCTGAGGATAATGACGATAACGAAGAGGAGTCTAAAAATTCTCACGGGCCAGCAACTTCAATCAGTTCAGCATACAAATTGTTGACACCATCTGTGAAGGTCCAGCTATTGATATACTTTATGCTCAAGTACGCTATGGAAATCTTGCTCTCTGAATCAAGTGTCATCACCACATACTATTTTAACTGGTCTACAAGCGCTGTGGCTATCTTTTTAGCGATTCTTGGGTGTACTGTTCTCCCTGTCAACGCCATTGTTGGAAGCTACATTACAAATTTATTCGAGGACAGGCAAATTTTGGTGGCATCTGAAATCATGGTCCTCATTGGTATAATCATGAGCTTCCGTTACACCCCTCACTACTCCGTTCCGCAGTACGTCTTGTCCGCTCTCATCACATTTGTGTTTGCTGAGGTCCTCGAAGGAGTGAACCTGTCGTTGCTTTCACGAGTAATGTCGTCGAGGCTCGCGCGAGGAACCTACAACGGCGGCCTCCTGTCGACAGAGGCCGGGACGCTGGCCCGTGTAGTTGCAGACGCGACGATTACCGCGGCGGGTTATCTAGGCCCGGACCTCCTGCTCAACATCACTCTGCTGCCACCTCTTGTCATCTGCATAGCCTCCCTCGTTGCAACATTCTGCACATACAACACCCTGTACTAAGGAAGTTGCCTCTCTCTGCACACTGTAGAGCCGGTTAAACTTTATTCATGCTCACATTGGGTTGTATTTTACATGTATCTGCATGCGGTAGCATTCTTTGTTTGTACAAGTCCTTAAACAGTTCAATAAAATGTTCTTCTGTAAATCCTACAGATGTTTTCTCGATGCATGTTTACAGTCCTTATGCATTTTACATATGTATTCATTTTTCCTATATCTGTAAAAAAAATTTCTTGATCTGTACCAATATTATGAGTTATGAGGTTGAAAAGACACATGCACCATGGAC
CDS Sequence
  • >LOC_Os04g48390.1
    ATGGTTAATTTTGGAAAGAAGTTGATGGCTGATCAAGTAGAAGAATGGAAAGGATACTATATTAACTACAAATTGATGAAGAAAATGTTGAAGCAATACGTTCAGCAGACCCAACTTGGTGGAAAAGATCGAGAGCAAGTACTTAAAGAGTTCTCAAGAATTCTTGATGAACAGATTGAAAGGATTGTACTTTTTCTGCTACAACAACAAGGCCACCTTGCTAATAGGATTGAGGAATTGGGAGAACAGCGTGCTGCTCTTCTAGAACAGCATGATATATCCCAAGTTTTTCAACTACGAGAGGCTTATAGAGAAGTTGGGCGAGATCTTATAAAGCTTCTCCGTTTTGTTGATATGAATGCTACTGGTATAAGGAAGATTCTAAAGAAATTCGATAAGCGCTTTGGCTATAGGTTTACAGACTATTATGTCACCACTCGTGCAAACCATCCTTATTCTCAGCTTCAACAAGTCTTTAAGCAAGTGGGGATTGTAGCTGTTGTAGGAGCTTTATCGCGCAACCTTGCATATCTGCAAGATCATGAAGGAAGTGTTTTGTCCATCTATGATCATCCATCAGTTACCTTAAAGGACCCTATCATAGACCAAGTAAATCATGCGGTACAAAAACTTACACATGCGACGAGTTTTCTGCAATTCTTAGGTCAACATGCACTTATCATCCAAGAGGACGTGCAAAGTGGGTCGGAGGATCTTGTCGATGATCAAAGTTACCATTTCATGTCTTTGATACTCAACCTAGTGAACACCTTTCTTTACATGGTGAACACATATATCATTGTGCCAACTGCTGATGACTACGCTGTAAGCCTTGGAGCTGCAGCGACAGTTTGTGGCGTAATCATCGGATCAATGGCAGTTGCTCAAGTGTTCTCCTCAGTTTATTTCAGTGCATGGTCAAATAGGTCATACTTCAGACCTCTTGTATTCAGTAGCATCATGTTATTCGCTGGGAATCTGCTATATGCATTAGCATATGACCTGAATTCTCTGACAGTTCTCCTGATTGGCCGGCTACTATGCGGGTTAGGCTCTGCAAGAGCTGTGAACCGTCGATATATCAGCGATTGTGTGCCTCTCAAAATCAGGCTACAGGCCTCTGCAGGATTCGTCAGTGCTAGTGCTCTTGGCATGGCATGTGGCCCTGCTCTTGCTGGTTTACTCCAGACAAGATTTAAGATATACTCACTCACTTTTGATCAGAGCACGTTGCCTGGGTGGGTCATGTGCATTGCTTGGCTTGTTTACTTGCTGTGGCTGTGGATATCATTCAAAGAGCCGGGACACTTTGCTAAGAGTTCAGACACGGCACAGCCTGCTGAATCTGGCCATCAAGTCAATGCTAATCTGGAGGAAGGTCTAGCGCAACCATTGCTTACAGGCTCAGAAGAGGGACAAGACCAAAATGCTGAGGATAATGACGATAACGAAGAGGAGTCTAAAAATTCTCACGGGCCAGCAACTTCAATCAGTTCAGCATACAAATTGTTGACACCATCTGTGAAGGTCCAGCTATTGATATACTTTATGCTCAAGTACGCTATGGAAATCTTGCTCTCTGAATCAAGTGTCATCACCACATACTATTTTAACTGGTCTACAAGCGCTGTGGCTATCTTTTTAGCGATTCTTGGGTGTACTGTTCTCCCTGTCAACGCCATTGTTGGAAGCTACATTACAAATTTATTCGAGGACAGGCAAATTTTGGTGGCATCTGAAATCATGGTCCTCATTGGTATAATCATGAGCTTCCGTTACACCCCTCACTACTCCGTTCCGCAGTACGTCTTGTCCGCTCTCATCACATTTGTGTTTGCTGAGGTCCTCGAAGGAGTGAACCTGTCGTTGCTTTCACGAGTAATGTCGTCGAGGCTCGCGCGAGGAACCTACAACGGCGGCCTCCTGTCGACAGAGGCCGGGACGCTGGCCCGTGTAGTTGCAGACGCGACGATTACCGCGGCGGGTTATCTAGGCCCGGACCTCCTGCTCAACATCACTCTGCTGCCACCTCTTGTCATCTGCATAGCCTCCCTCGTTGCAACATTCTGCACATACAACACCCTGTACTAA
Protein Sequence
  • >LOC_Os04g48390.1
    MVNFGKKLMADQVEEWKGYYINYKLMKKMLKQYVQQTQLGGKDREQVLKEFSRILDEQIERIVLFLLQQQGHLANRIEELGEQRAALLEQHDISQVFQLREAYREVGRDLIKLLRFVDMNATGIRKILKKFDKRFGYRFTDYYVTTRANHPYSQLQQVFKQVGIVAVVGALSRNLAYLQDHEGSVLSIYDHPSVTLKDPIIDQVNHAVQKLTHATSFLQFLGQHALIIQEDVQSGSEDLVDDQSYHFMSLILNLVNTFLYMVNTYIIVPTADDYAVSLGAAATVCGVIIGSMAVAQVFSSVYFSAWSNRSYFRPLVFSSIMLFAGNLLYALAYDLNSLTVLLIGRLLCGLGSARAVNRRYISDCVPLKIRLQASAGFVSASALGMACGPALAGLLQTRFKIYSLTFDQSTLPGWVMCIAWLVYLLWLWISFKEPGHFAKSSDTAQPAESGHQVNANLEEGLAQPLLTGSEEGQDQNAEDNDDNEEESKNSHGPATSISSAYKLLTPSVKVQLLIYFMLKYAMEILLSESSVITTYYFNWSTSAVAIFLAILGCTVLPVNAIVGSYITNLFEDRQILVASEIMVLIGIIMSFRYTPHYSVPQYVLSALITFVFAEVLEGVNLSLLSRVMSSRLARGTYNGGLLSTEAGTLARVVADATITAAGYLGPDLLLNITLLPPLVICIASLVATFCTYNTLY*