Gene Details:

Functional Descriptions:

  • The MOF1 mutant has a delayed transition from the spikelet to the floral meristem, inducing the formation of extra lemma-like and palea-like organs.
  • Taken together, our results reveal that MOF1 plays an important role in the regulation of organ identity and spikelet determinacy in rice.
  • We used map-based cloning to identify the MOF1 locus and confirmed our identification by complementation and by generating new MOF1 alleles using CRISPR-Cas9 gene editing.
  • MOF1 encodes a MYB domain protein with the typical ethylene response factor-associated amphiphilic repression (EAR) motifs, is expressed in all organs and tissues, and has a strong repression effect.
  • MOF1 localizes to the nucleus and interacts with TOPLESS-RELATED PROTEINs (TPRs) to possibly repress the expression of downstream target genes.
  • Due to the increase in the number of floral organs and development of extra transformed palea/marginal region of the palea (mrp)-like organs, some MFS2 spikelets had a tendency to produce two florets.
  • These defects implied that the MFS2 mutation caused abnormal specification of palea identity and partial loss of spikelet determination.
  • The results indicate that MFS2 acts as a repressor that regulates floral organ identities and spikelet meristem determinacy in rice by forming a repression complex with rice TPL/TPR proteins.
  • In the MFS2 mutant, specification of palea identity was severely disturbed and showed degradation or transformation into a lemma-like organ, and the number of all floral organs was increased to varying degrees.

Literature:

Gene Resources:

  • NCBI ID:
  • UniProt accessions:

Sequences:

cDNA Sequence
  • >LOC_Os04g47890.1
    ACACCCCCTCTAGCGTTGAGAGAGAGAGAGAGAGAGGATGTTTTGAGAGAGCACAGCTAGAAGCTTGGCTTCTCGCAACAAGTGCTGCATCTCTAGTGAGCGAGTGAGTGAGAGAGACAGCCTTGGCCTTGGCGTTGGTGGTGTAGCTCCAGCCTCCAAGCATCCATCCATGCTTACATAAGTCAGACAGGGAGGGAGGACCGGAAGGAGGAGGAGAGAAAAAGCGAAGCTTTGGTTCTTTCTTTGCACCACCGGTTGCCTCCGTTGATCTCCTTGTCGATCGCCGGAAGCAAACCGTTGTTGGTGATGGGCAGCGGCGGCGGTGGCTGCGGCAGGAACGGCGCTGTGAGGCAGTACATCAGGTCCAAGGTGCCGAGGCTGAGGTGGACCGGGGAGCTCCACTGCAGCTTCGTCCAAGCCATCGAATTCCTCGGTGGCCAAGACAAGGCTACACCTAAGCTCATTCTTCAGCTCATGGGGGTGAAGGGGCTGACCATATCTCATGTCAAGAGCCACCTCCAGATGTACAGATGCTCCAGGCTCGGCTCCCATGGCACGGGAAGGAGATCAGAGATGCAACCACAGCTGCAAAGGAAGCACTCATGTGGTGCTGATGAGCAAGTCCCCAGAGAATTCCTGTGCCCCCCTCTGAAAAGGACCAGGATGGGGACAGAAGCCACATACAAAGGCATGCAAGGAAGCCAAGGAATCAGTGAGATGAGGACTACTGGCACCCAGTACTGCATTGATGATTACATGCAAGCCATGGCAATGGAGAGGAGAATAAAGGAGGAGGGCCTCAGATGGCAGAGGGATGCTGCTGCTGCTGCTGCTGCAGATGGTGGTGCTGCTGCTTCCAACCTCCAAACCGTGGGATGTTCGGTGCAAGAATCTGACCCCTTTAAGATCATCAAACCAGAAGTGCACCATCTTGGTCCCGTGTTGAAGCTGCAATGCTCCAAGGTGGAGAACAGTGGATTCATCTCCAGCAGCACCGGCACGGCTGCAAGGGATCAACCGGAGCCGCCGCCGCTGGAGAAATGTTCGCTGTCACTCTCCCTCGGTCCAGACCCCAAATGCATGCCGGCGATCGCCTCGTCGCCGAGCGAAAGCAGCTGCATCCTCTCGTCGTCGTCCAGGAGCTTCAGCGACTGCTCCGGGAACTCAGGTTGTCTTGTTGCCCCGGGTGTGAACTTGGAACTCTCCATGTCCATCTGTGGATCTTAGACCTTGGTTCCAGTAATCCAGTTTTTGTACATCAGTCAGTCTCACATGTCGGTTTTGAACTTTTAATCACCTGCTTCAGTGTTGATACGGTGATACCCTGATCATGTTAGGAAGTTAGCCAATTGCAAGATGTCAGTGTAGGGCCTTGAGTATGCAGTGCAATGATCC
CDS Sequence
  • >LOC_Os04g47890.1
    ATGGGCAGCGGCGGCGGTGGCTGCGGCAGGAACGGCGCTGTGAGGCAGTACATCAGGTCCAAGGTGCCGAGGCTGAGGTGGACCGGGGAGCTCCACTGCAGCTTCGTCCAAGCCATCGAATTCCTCGGTGGCCAAGACAAGGCTACACCTAAGCTCATTCTTCAGCTCATGGGGGTGAAGGGGCTGACCATATCTCATGTCAAGAGCCACCTCCAGATGTACAGATGCTCCAGGCTCGGCTCCCATGGCACGGGAAGGAGATCAGAGATGCAACCACAGCTGCAAAGGAAGCACTCATGTGGTGCTGATGAGCAAGTCCCCAGAGAATTCCTGTGCCCCCCTCTGAAAAGGACCAGGATGGGGACAGAAGCCACATACAAAGGCATGCAAGGAAGCCAAGGAATCAGTGAGATGAGGACTACTGGCACCCAGTACTGCATTGATGATTACATGCAAGCCATGGCAATGGAGAGGAGAATAAAGGAGGAGGGCCTCAGATGGCAGAGGGATGCTGCTGCTGCTGCTGCTGCAGATGGTGGTGCTGCTGCTTCCAACCTCCAAACCGTGGGATGTTCGGTGCAAGAATCTGACCCCTTTAAGATCATCAAACCAGAAGTGCACCATCTTGGTCCCGTGTTGAAGCTGCAATGCTCCAAGGTGGAGAACAGTGGATTCATCTCCAGCAGCACCGGCACGGCTGCAAGGGATCAACCGGAGCCGCCGCCGCTGGAGAAATGTTCGCTGTCACTCTCCCTCGGTCCAGACCCCAAATGCATGCCGGCGATCGCCTCGTCGCCGAGCGAAAGCAGCTGCATCCTCTCGTCGTCGTCCAGGAGCTTCAGCGACTGCTCCGGGAACTCAGGTTGTCTTGTTGCCCCGGGTGTGAACTTGGAACTCTCCATGTCCATCTGTGGATCTTAG
Protein Sequence
  • >LOC_Os04g47890.1
    MGSGGGGCGRNGAVRQYIRSKVPRLRWTGELHCSFVQAIEFLGGQDKATPKLILQLMGVKGLTISHVKSHLQMYRCSRLGSHGTGRRSEMQPQLQRKHSCGADEQVPREFLCPPLKRTRMGTEATYKGMQGSQGISEMRTTGTQYCIDDYMQAMAMERRIKEEGLRWQRDAAAAAAADGGAAASNLQTVGCSVQESDPFKIIKPEVHHLGPVLKLQCSKVENSGFISSSTGTAARDQPEPPPLEKCSLSLSLGPDPKCMPAIASSPSESSCILSSSSRSFSDCSGNSGCLVAPGVNLELSMSICGS*