Information report for HTD1;OsCCD7
Gene Details
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Functional Descriptions
- The genetic analysis of the htd-1 mutant indicated that the phenotypes of high tillering and dwarf were controlled by a recessive gene, termed HTD1.
- The HTD1 gene is expressed in both shoot and root tissues.
- Loss of function of HTD2 resulted in a significantly increased expression of HTD1, D10 and D3, which were involved in the strigolactone biosynthetic pathway.
- By evaluating Pro(HTD1):GUS expression, we found that the HTD1 gene is mainly expressed in vascular bundle tissues throughout the plant.
- The HIGH-TILLERING DWARF1 (HTD1) gene encodes an ortholog of Arabidopsis MAX3.
- Complementation analyses for HTD1 confirm that the defect in HTD1 is responsible for both high-tillering and dwarf phenotypes in the HTD1 mutant.
- Auxin induction of HTD1 expression suggests that auxin may regulate rice tillering partly through upregulation of HTD1 gene transcription.
- Restoration of dwarf phenotype after the removal of axillary buds indicates that the dwarfism of the HTD1 mutant may be a consequence of excessive tiller production.
- Moreover, D10 expression is upregulated in six branching mutants, d3, d10, d14, d17, d27 and high tillering dwarf (HTD1).
- Moreover, the mRNA levels of D3, HTD1 and D10, which are orthologs of Arabidopsis MAX2/ORE9, MAX3 and MAX4, respectively, increased during cell death.
- A substitution mutation in OsCCD7 cosegregates with dwarf and increased tillering phenotype in rice.
- Targeted mutations in five carotenoid catabolism genes failed to boost carotenoid accumulation in rice seeds, but produced dwarf and high tillering mutants when OsCCD7 gene was knocked out.
- However, transgenic plants with homozygous or bi-allelic mutations to the OsCCD7 gene were extremely dwarfish with more tillers and lower seed setting than other transgenic or nontransgenic plants.
- We found that the HTD1 gene had been widely utilized and co-selected with Semidwarf 1 (SD1), both contributing to the improvement of plant architecture in modern rice varieties since the Green Revolution in the 1960s.
Functional Keywords
- tillering , root , strigolactone , shoot , vascular-bundle , tiller , auxin , dwarf , cell-death , branching , seed , architecture , plant-architecture
Literature and News
- Characterizations and fine mapping of a mutant gene for high tillering and dwarf in rice (Oryza sativa L.) . DOI: 10.1007/s00425-005-0007-0 ; PMID: 16021500
- Identification and characterization of HTD2: a novel gene negatively regulating tiller bud outgrowth in rice . DOI: 10.1007/s00425-009-0975-6 ; PMID: 19579033
- Rice tillering dwarf mutant dwarf3 has increased leaf longevity during darkness-induced senescence or hydrogen peroxide-induced cell death . DOI: 10.1266/ggs.82.361 ; PMID: 17895586
- The rice HIGH-TILLERING DWARF1 encoding an ortholog of Arabidopsis MAX3 is required for negative regulation of the outgrowth of axillary buds . DOI: 10.1111/j.1365-313X.2006.02916.x ; PMID: 17092317
- DWARF10, an RMS1/MAX4/DAD1 ortholog, controls lateral bud outgrowth in rice . DOI: 10.1111/j.1365-313X.2007.03210.x ; PMID: 17655651
- Carotenoid oxygenases involved in plant branching catalyse a highly specific conserved apocarotenoid cleavage reaction . DOI: 10.1042/BJ20080568 ; PMID: 18637791
- A substitution mutation in OsCCD7 cosegregates with dwarf and increased tillering phenotype in rice . DOI: 10.1007/s12041-014-0389-5 ; PMID: 25189234
- Knocking out of carotenoid catabolic genes in rice fails to boost carotenoid accumulation, but reveals a mutation in strigolactone biosynthesis . DOI: 10.1007/s00299-017-2172-6 ; PMID: 28676963
- A Strigolactone Biosynthesis Gene Contributed to the Green Revolution in Rice . DOI: 10.1016/j.molp.2020.03.009 ; PMID: 32222483
- Characterization of dwarf and narrow leaf (dnl-4) mutant in rice . DOI: 10.1080/15592324.2020.1849490 ; PMID: 33300429
Gene Resources
- UniProt: Q7XU29
- EMBL: AK109771, AL663000, AP008210
- AlphaFoldDB: Q7XU29
- EnsemblPlants: Os04t0550600-01
- Gramene: Os04t0550600-01
- KEGG: dosa:Os04g0550600
Sequences
cDNA Sequence
- >LOC_Os04g46470.1
AACGACCGAAGGAGGCCAAGTCCAAAGATGGCAACACAAGCGATTGCACCGATGCACGCCGCCGTCGTGCACCGCCACCACGTTCTACCACCCCGCCGCTGCGTGCGCCGCCGTGGCGTCTTCGTCCGCGCCTCGGCCGCCGCCGCCGCCGCCGCCGCCGAGACGGACACGCTGTCCGCGGCCTTCTGGGACTACAACCTCCTCTTCCGGTCGCAGCGCGACGAGTGCCTCGACTCCATCCCGCTCCGCGTCACCGAGGGCGCGATCCCGCCCGACTTCCCGGCCGGCACCTACTACCTCGCCGGGCCGGGCATCTTCTCCGACGACCACGGCTCCACCGTCCACCCCCTCGACGGCCACGGCTACCTCCGCTCCTTCCGCTTCCGGCCCGGCGACCGCACCATCCACTACTCCGCGCGGTTCGTGGAGACGGCGGCGAAGAGGGAGGAGAGCCGGGACGGCGCGTCGTGGCGGTTCACGCACCGGGGGCCCTTCTCCGTGCTGCAGGGCGGGAAGAAGGTGGGCAATGTGAAGGTGATGAAGAACGTGGCCAACACCAGCGTGCTGCGGTGGGGCGGCCGGCTGCTCTGCCTCTGGGAGGGCGGCCAGCCGTACGAGGTTGACCCCCGGACGCTCGAGACCGTCGGCCCGTTCGACCTGCTCGGCCTCGCCGCCGCCGACGACAACAAGGCAACGAACGCGTCTGCAGCACGACGGCCGTGGCTGCAGGAGGCCGGCCTCGACGCCGCCGCGCGCCTGCTGCGCCCTGTTCTTAGCGGGGTGTTCGACATGCCGGGCAAGAGGCTGCTGGCGCACTACAAGATCGACCCGCGGCGGGGGCGTCTGCTGATGGTCGCCTGCAACGCCGAGGACATGCTCCTCCCGCGATCCCACTTCACTTTCTACGAGTTCGACGCCCACTTCGACCTCGTCCAGAAGCGTGAGTTCGTCGTGCCGGACCACCTCATGATCCACGACTGGGCCTTCACCGACACCCACTACATCCTCCTCGGCAACAGGATCAAGCTCGACATCCCCGGATCGCTGCTGGCATTGACGGGCACTCACCCGATGATCGCGGCGCTGGCCGTGGACCCGAGAAGGCAGTCGACGCCGGTGTACCTGCTTCCGCGCTCCCCGGAGACCGAGGCGGGCGGCCGCGACTGGAGCGTGCCGATCGAGGCGCCGTCGCAGATGTGGTCCGTGCACGTCGGCAACGCGTTCGAGGAGGCGAACCGCCGGGGCGGCCTCGACGTCCGGCTGCACATGTCAAGCTGCTCCTACCAGTGGTTCCATTTCCACAGGATGTTTGGTTACAATTGGCACCACAAGAAGCTGGACCCGTCGTTCATGAACGCGGCGAAGGGAAAGGAGTGGCTGCCTCGCCTCGTTCAGGTGGCCATCGAGCTCGACAGGACGGGAGAGTGCCGGAGGTGCTCAGTCAGGAGGCTGTCCGATCAGCACGCCAGGCCGGCGGACTTCCCGGCGATAAACCCAAGCTACGCCAACCAGAGGAACCGGTTCGTCTACGCCGGCGCCGCGTCCGGCTCCCGCAGATTCCTCCCGTACTTCCCGTTCGACAGCGTGGTGAAGGTCGACGTCTCCGATGGATCGGCGCGGTGGTGGTCTACCGACGGGCGCAAGTTCGTCGGCGAGCCGGTCTTCGTCCCGACCGGCGGCGGAGAGGATGGTGGCTATGTTCTTCTTGTAGAGTATGCAGTCTCCAAGCACAGATGCCATCTAGTGGTGCTGGATGCAAAGAAGATAGGGACAGAGAATGCACTTGTGGCAAAACTAGAGGTGCCAAAGAACCTCACTTTTCCAATGGGATTCCATGGTTTCTGGGGAGATGAATGAGCATAGAGCAAGCATCAGATCCAGTCTAACTCTGGAGAGAAATTGTCTTGAAAAGGCAAGAATTTTGCCTCGTGTATTGATAAAAGAGAGTTTTGTGATAATCTGTACATGGTGGAGAGGAATTATCAGGGGAACCAAATAACTACTGTACATCCAGTCTCGGAATGAAAATTCGTCTCACCAATTCATTAAAATCCTGAAATACTAATGTTTTAAGGCAATAATTCATAGTATTCCCATAGGGTAAACATATAAGACCTCCACTGAGAATCTTGATGTCTTGGCAATTTTAAAATGAAAGTTCTTGAACTGACAACTAAGAATGATACCATCCATGATCTTAACCCATTCGACAAGAATTTAAAAGGCTAGAACATATCCTGTACACAGCTAACAGACCACCAAATGTGAAGTCGACGAATTTTTGGCATCACTTAATGCGAGCCACAGTCACCACAATGAAGACGAACACTGCGAGCATGAAGGACATTCTCACTGTATCGTCATACAAATCATAGTCCTCACTCTGAGCTTGAAAGAGCATGTTCACAAGCTTAAGTATGCTCTCAAGGAATTCCTTGATCTTTGCTTGGGTGGTTTCCATGACCCACTTCATCACTCCAGGAGAAGCAGAAGA
CDS Sequence
- >LOC_Os04g46470.1
ATGGCAACACAAGCGATTGCACCGATGCACGCCGCCGTCGTGCACCGCCACCACGTTCTACCACCCCGCCGCTGCGTGCGCCGCCGTGGCGTCTTCGTCCGCGCCTCGGCCGCCGCCGCCGCCGCCGCCGCCGAGACGGACACGCTGTCCGCGGCCTTCTGGGACTACAACCTCCTCTTCCGGTCGCAGCGCGACGAGTGCCTCGACTCCATCCCGCTCCGCGTCACCGAGGGCGCGATCCCGCCCGACTTCCCGGCCGGCACCTACTACCTCGCCGGGCCGGGCATCTTCTCCGACGACCACGGCTCCACCGTCCACCCCCTCGACGGCCACGGCTACCTCCGCTCCTTCCGCTTCCGGCCCGGCGACCGCACCATCCACTACTCCGCGCGGTTCGTGGAGACGGCGGCGAAGAGGGAGGAGAGCCGGGACGGCGCGTCGTGGCGGTTCACGCACCGGGGGCCCTTCTCCGTGCTGCAGGGCGGGAAGAAGGTGGGCAATGTGAAGGTGATGAAGAACGTGGCCAACACCAGCGTGCTGCGGTGGGGCGGCCGGCTGCTCTGCCTCTGGGAGGGCGGCCAGCCGTACGAGGTTGACCCCCGGACGCTCGAGACCGTCGGCCCGTTCGACCTGCTCGGCCTCGCCGCCGCCGACGACAACAAGGCAACGAACGCGTCTGCAGCACGACGGCCGTGGCTGCAGGAGGCCGGCCTCGACGCCGCCGCGCGCCTGCTGCGCCCTGTTCTTAGCGGGGTGTTCGACATGCCGGGCAAGAGGCTGCTGGCGCACTACAAGATCGACCCGCGGCGGGGGCGTCTGCTGATGGTCGCCTGCAACGCCGAGGACATGCTCCTCCCGCGATCCCACTTCACTTTCTACGAGTTCGACGCCCACTTCGACCTCGTCCAGAAGCGTGAGTTCGTCGTGCCGGACCACCTCATGATCCACGACTGGGCCTTCACCGACACCCACTACATCCTCCTCGGCAACAGGATCAAGCTCGACATCCCCGGATCGCTGCTGGCATTGACGGGCACTCACCCGATGATCGCGGCGCTGGCCGTGGACCCGAGAAGGCAGTCGACGCCGGTGTACCTGCTTCCGCGCTCCCCGGAGACCGAGGCGGGCGGCCGCGACTGGAGCGTGCCGATCGAGGCGCCGTCGCAGATGTGGTCCGTGCACGTCGGCAACGCGTTCGAGGAGGCGAACCGCCGGGGCGGCCTCGACGTCCGGCTGCACATGTCAAGCTGCTCCTACCAGTGGTTCCATTTCCACAGGATGTTTGGTTACAATTGGCACCACAAGAAGCTGGACCCGTCGTTCATGAACGCGGCGAAGGGAAAGGAGTGGCTGCCTCGCCTCGTTCAGGTGGCCATCGAGCTCGACAGGACGGGAGAGTGCCGGAGGTGCTCAGTCAGGAGGCTGTCCGATCAGCACGCCAGGCCGGCGGACTTCCCGGCGATAAACCCAAGCTACGCCAACCAGAGGAACCGGTTCGTCTACGCCGGCGCCGCGTCCGGCTCCCGCAGATTCCTCCCGTACTTCCCGTTCGACAGCGTGGTGAAGGTCGACGTCTCCGATGGATCGGCGCGGTGGTGGTCTACCGACGGGCGCAAGTTCGTCGGCGAGCCGGTCTTCGTCCCGACCGGCGGCGGAGAGGATGGTGGCTATGTTCTTCTTGTAGAGTATGCAGTCTCCAAGCACAGATGCCATCTAGTGGTGCTGGATGCAAAGAAGATAGGGACAGAGAATGCACTTGTGGCAAAACTAGAGGTGCCAAAGAACCTCACTTTTCCAATGGGATTCCATGGTTTCTGGGGAGATGAATGA
Protein Sequence
- >LOC_Os04g46470.1
MATQAIAPMHAAVVHRHHVLPPRRCVRRRGVFVRASAAAAAAAAETDTLSAAFWDYNLLFRSQRDECLDSIPLRVTEGAIPPDFPAGTYYLAGPGIFSDDHGSTVHPLDGHGYLRSFRFRPGDRTIHYSARFVETAAKREESRDGASWRFTHRGPFSVLQGGKKVGNVKVMKNVANTSVLRWGGRLLCLWEGGQPYEVDPRTLETVGPFDLLGLAAADDNKATNASAARRPWLQEAGLDAAARLLRPVLSGVFDMPGKRLLAHYKIDPRRGRLLMVACNAEDMLLPRSHFTFYEFDAHFDLVQKREFVVPDHLMIHDWAFTDTHYILLGNRIKLDIPGSLLALTGTHPMIAALAVDPRRQSTPVYLLPRSPETEAGGRDWSVPIEAPSQMWSVHVGNAFEEANRRGGLDVRLHMSSCSYQWFHFHRMFGYNWHHKKLDPSFMNAAKGKEWLPRLVQVAIELDRTGECRRCSVRRLSDQHARPADFPAINPSYANQRNRFVYAGAASGSRRFLPYFPFDSVVKVDVSDGSARWWSTDGRKFVGEPVFVPTGGGEDGGYVLLVEYAVSKHRCHLVVLDAKKIGTENALVAKLEVPKNLTFPMGFHGFWGDE*