OsYABBY5;OsYAB3

Gene Details:

Here, we show that mutation of the rice (Oryza sativa) gene TONGARI-BOUSHI1 (TOB1) results in pleiotropic phenotypes in spikelets, such as the formation of a cone-shaped organ instead of the lemma or palea, the development of two florets in a spikelet, or premature termination of the floret meristem, in addition to reduced growth of the lemma or palea and elongation of the awn.
No TOB1 expression is detected in the meristem, so TOB1 may act non-cell autonomously to maintain proper meristem organization and is therefore likely to play an important role in rice spikelet development.
TOB1 encodes a YABBY protein, which is closely related to FILAMENTOUS FLOWER in Arabidopsis thaliana, and is expressed in the lateral organ primordia without any patterns of polarization.
RNAi knockdown of TOB2 or TOB3 in the TOB1 mutant caused abnormal spikelet development.
Furthermore, simultaneous knockdown of both TOB2 and TOB3 in TOB1 affected not only spikelet, but also inflorescence development.

cDNA Sequence

>LOC_Os04g45330.1
ATCCGCTAGCTTCATCTCCACTAGTCTCATCCCTCCTTCTCCCTCTCCTCGTTGAACACCGCACTATCAAATCACCCAGCTGAAAGCCAAGACCGCGCGCGCCGGCCGGCCGGGCAGGGATCACCTAGCTAGGATACGCGCCATGATGTCGTCGGCGCCGGAGACGTTCTCGCTGGACCACCTCTCGCAGCATCAGCAGCAGCAGCCGCCGCCGCTCGCCGAGCAGGAGCAGCTCTGCTACGTGCACTGCAACTTCTGCGACACCATCCTCGCGGTGGGCGTGCCGTGTAGCAGCCTGTTCAAGACGGTCACCGTGCGGTGCGGCCACTGCGCCAACCTGCTCTCCGTCAACCTCCGCGGCCTCCTGCTCCCGGCCGCCGCCTCCACCGCCAACCAGCTCCCCTTCGGCCAGGCTCTCCTCTCCCCCACCTCACCGCACGGCCTCCTTGATGAGGTGCCGTCATTCCAAGCGCCGGCGAGCCTAATGACCGAACAGGCGAGTCCCAACGTGAGCAGCATCACGAGCAGCAACAGCAGCTGTGCTAACAACGCGCCGGCGACGTCGATGGCGTCGGCGGCCAACAAGGCTACCCAGCGAGAGCCGCAGCAGCCGAAGAACGCGCCGTCAGCCAACAGGACTTCAGAGAAGCGGCAGAGGGTTCCCTCGGCATACAACCGATTCATCAAAGATGAGATACAACGCATCAAGGCAAGCAATCCGGACATCACCCACAGGGAGGCTTTCAGCGCTGCTGCCAAGAACTGGGCCCATTTCCCACACATCCATTTTGGCCTGATGCCGGACCAGGGCCTGAAGAAGACCGGCATACAAAGCCAGGACGGAGCTGGGGAATGCATGCTCTTCAAGGACGGTCTCTACGCTGCAGCCGCCGCCGCTGCAGCAGCCACAGCAGCATCTAGCATGGGTGTCACTCCATTCTAACGCCTCAGATCAATGCACGATCTGGCTTAAAAAAAACTTTGTGTCCCACCCTGTAGTTAGGGTCCTGCATGTCTCGATCTCCAGATATGATCTCCGGTTGCTTGTTAACTGAAATGTTCCTCTATATATGGACGATCATGGCACCATATGCATGGCATCTTGGCATGCATCGATCGTATCTAAGTCCTTATCTATGTGTAATTCTAGTTTTCTCAGTTTCTTCATGTATTTTCCCCTTAATTTTTCCTATTCCTATTCGCCTGTACAGAGGCTGCTAGCTGTGCGTGCTGCCTCTTATATATCTTATGGACATAACTAGATCGATTTGGTTTAATTACCAGGTGATCATTTGAAATCAAATCGTCTGATTCTTTTAGGGGTGGGGAGTTGAAACCCTTTTCA

CDS Sequence

>LOC_Os04g45330.1
ATGATGTCGTCGGCGCCGGAGACGTTCTCGCTGGACCACCTCTCGCAGCATCAGCAGCAGCAGCCGCCGCCGCTCGCCGAGCAGGAGCAGCTCTGCTACGTGCACTGCAACTTCTGCGACACCATCCTCGCGGTGGGCGTGCCGTGTAGCAGCCTGTTCAAGACGGTCACCGTGCGGTGCGGCCACTGCGCCAACCTGCTCTCCGTCAACCTCCGCGGCCTCCTGCTCCCGGCCGCCGCCTCCACCGCCAACCAGCTCCCCTTCGGCCAGGCTCTCCTCTCCCCCACCTCACCGCACGGCCTCCTTGATGAGGTGCCGTCATTCCAAGCGCCGGCGAGCCTAATGACCGAACAGGCGAGTCCCAACGTGAGCAGCATCACGAGCAGCAACAGCAGCTGTGCTAACAACGCGCCGGCGACGTCGATGGCGTCGGCGGCCAACAAGGCTACCCAGCGAGAGCCGCAGCAGCCGAAGAACGCGCCGTCAGCCAACAGGACTTCAGAGAAGCGGCAGAGGGTTCCCTCGGCATACAACCGATTCATCAAAGATGAGATACAACGCATCAAGGCAAGCAATCCGGACATCACCCACAGGGAGGCTTTCAGCGCTGCTGCCAAGAACTGGGCCCATTTCCCACACATCCATTTTGGCCTGATGCCGGACCAGGGCCTGAAGAAGACCGGCATACAAAGCCAGGACGGAGCTGGGGAATGCATGCTCTTCAAGGACGGTCTCTACGCTGCAGCCGCCGCCGCTGCAGCAGCCACAGCAGCATCTAGCATGGGTGTCACTCCATTCTAA

Protein Sequence

>LOC_Os04g45330.1
MMSSAPETFSLDHLSQHQQQQPPPLAEQEQLCYVHCNFCDTILAVGVPCSSLFKTVTVRCGHCANLLSVNLRGLLLPAAASTANQLPFGQALLSPTSPHGLLDEVPSFQAPASLMTEQASPNVSSITSSNSSCANNAPATSMASAANKATQREPQQPKNAPSANRTSEKRQRVPSAYNRFIKDEIQRIKASNPDITHREAFSAAAKNWAHFPHIHFGLMPDQGLKKTGIQSQDGAGECMLFKDGLYAAAAAAAAATAASSMGVTPF*