Gene Details:

Functional Descriptions:

  • OsClo5 functions as a transcriptional co-repressor by interacting with OsDi19-5 to negatively affect salt stress tolerance in rice seedlings.
  • In addition, OsClo5 enhanced the inhibitory effect of OsDi19-5 in the tobacco transient system, which was confirmed by qRT-PCR analysis in rice seedlings under salt stress.
  • Thereby, biochemical and molecular genetic experiments were carried out, the results revealed that OsClo5 had was able to bind calcium and phospholipids in vitro and localized in the nucleus and endoplasmic reticulum in rice protoplasts.
  • Overexpression of OsClo5 decreased cold tolerance, and RNAi lines of OsClo5 had higher survival than WT seedlings.
  • OsClo5 is a constitutively expressed caleosin protein that regulates plant cold resistance through inhibition of jasmonate signalling and JA synthesis.
  • OsClo5 inhibited one jasmonate biosynthetic gene and several jasmonate ZIM domain (JAZ) genes, which were upregulated in response to cold stress.
  • Overexpression lines and RNAi lines of OsClo5 were subjected to cold stress and recovery to measure electrolyte leakage and survival rate.

Literature:

Gene Resources:

  • NCBI ID: X89891
  • UniProt accessions:

Sequences:

cDNA Sequence
  • >LOC_Os04g43200.1
    GCTGCTCTCTCCTCGCGTATAAGAACAGGGGCGCTGCTGGGCCGGGCGTTTGCAATTTTGCAAAGCGAGAATTCCACACAGGTTACACCAGTATATACGCATCTTGCTAAACCGACTACTGATCGAGATCGCTATGGCGGAGGAGGCGGCTAGCAAGGCAGCGCCGACCGATGCGCTGTCGTCCGTGGCGGCGGAGGCGCCGGTGACGAGAGAACGGCCGGTCCGAGCGGACTTGGAAGTGCAGATTCCGAAGCCCTATTTGGCCCGAGCTCTGGTTGCTCCGGACGTGTACCATCCTGAAGGAACCGAGGGGCGTGACCACCGGCAGATGAGTGTGCTGCAGCAGCATGTGGCTTTCTTCGACCTGGATGGCGACGGTATCGTTTATCCATGGGAAACTTATGGAGGACTACGGGAATTGGGCTTCAACGTGATTGTTTCGTTCTTTTTGGCGATAGCCATAAACGTTGGTCTAAGCTACCCAACTCTGCCAAGCTGGATACCATCTCTCCTGTTCCCTATACACATAAAAAACATCCACAGGGCTAAGCACGGCAGCGATAGCTCGACGTACGACAACGAGGGAAGGTTTATGCCGGTCAATTTCGAGAGCATCTTCAGCAAGAACGCCCGCACGGCGCCGGACAAGCTCACGTTCGGCGATATCTGGCGGATGACCGAAGGCCAAAGGGTGGCGCTCGACTTGCTTGGGAGGATCGCGAGTAAGGGGGAGTGGATATTGCTCTACGTGCTTGCGAAAGATGAGGAAGGATTCCTCAGGAAGGAGGCTGTTCGCCGCTGCTTCGATGGGAGCCTATTCGAGTCGATTGCCCAGCAGAGAAGGGAGGCACATGAGAAGCAGAAGTAGCCTCCTAATTTCATCGTCCCGGGACCTGGGATGTGCTTGATTGCTTGTGTGTGTTGTTGTGTGGACTATAGCTATAGCCACATCATGTTTGTCCATCTGAAAAAACAATGGAAATAAGGTTTACCGGTTGGAACATACATTATGTACTATCCATGTGATTATTGAAATGTGTCTGTAACCTGAAAGTGTGATTGACATATAAAATTCTGTGATTGAAGTAAAGGTAAGCATTACTAAATGTGTA
  • >LOC_Os04g43200.2
    GCTGCTCTCTCCTCGCGTATAAGAACAGGGGCGCTGCTGGGCCGGGCGTTTGCAATTTTGCAAAGCGAGAATTCCACACAGGTTACACCAGTATATACGCATCTTGCTAAACCGACTACTGATCGAGATCGCTATGGCGGAGGAGGCGGCTAGCAAGGCAGCGCCGACCGATGCGCTGTCGTCCGTGGCGGCGGAGGCGCCGGTGACGAGAGAACGGCCGGTCCGAGCGGACTTGGAAGTGCAGATTCCGAAGCCCTATTTGGCCCGAGCTCTGGTTGCTCCGGACGTGTACCATCCTGAAGGAACCGAGGGGCGTGACCACCGGCAGATGAGTGTGCTGCAGCAGCATGTGGCTTTCTTCGACCTGGATGGCGACGGTATCGTTTATCCATGGGAAACTTATGGAGGACTACGGGAATTGGGCTTCAACGTGATTGTTTCGTTCTTTTTGGCGATAGCCATAAACGTTGGTCTAAGCTACCCAACTCTGCCAAGCTGGATACCATCTCTCCTGTTCCCTATACACATAAAAAACATCCACAGGGCTAAGCACGGCAGCGATAGCTCGACGTACGACAACGAGGGAAGGTTTATGCCGGTCAATTTCGAGAGCATCTTCAGCAAGAACGCCCGCACGGCGCCGGACAAGCTCACGTTCGGCGATATCTGGCGGATGACCGAAGGCCAAAGGGTGGCGCTCGACTTGCTTGGGAGGATCGCGAGTAAGGGGGAGTGGATATTGCTCTACGTGCTTGCGAAAGATGAGGAAGGATTCCTCAGGAAGGAGGCTGTTCGCCGCTGCTTCGATGGGAGCCTATTCGAGTCGATTGCCCAGCAGAGAAGGGAGGCACATGAGAAGCAGAAGTAGCCTCCTAATTTCATCGTCCCGGGACCTGGGATGTGCTTGATTGCTTGTGTGTGTTGTTGTGTGGACTATAGCTATAGCCACATCATGTTTGTCCATCTGAAAAAACAATGGAAATAAGGTTTACCGGTTGGAACATACATTATGTACTATCCATGTGATTATTGAAATGTGTCTGTAACCTGAAAGTGTGATTGACATATAAAATTCTGTGATTGAAGTAAAGGTAAGCATTACTAAATGTGTA
CDS Sequence
  • >LOC_Os04g43200.1
    ATGGCGGAGGAGGCGGCTAGCAAGGCAGCGCCGACCGATGCGCTGTCGTCCGTGGCGGCGGAGGCGCCGGTGACGAGAGAACGGCCGGTCCGAGCGGACTTGGAAGTGCAGATTCCGAAGCCCTATTTGGCCCGAGCTCTGGTTGCTCCGGACGTGTACCATCCTGAAGGAACCGAGGGGCGTGACCACCGGCAGATGAGTGTGCTGCAGCAGCATGTGGCTTTCTTCGACCTGGATGGCGACGGTATCGTTTATCCATGGGAAACTTATGGAGGACTACGGGAATTGGGCTTCAACGTGATTGTTTCGTTCTTTTTGGCGATAGCCATAAACGTTGGTCTAAGCTACCCAACTCTGCCAAGCTGGATACCATCTCTCCTGTTCCCTATACACATAAAAAACATCCACAGGGCTAAGCACGGCAGCGATAGCTCGACGTACGACAACGAGGGAAGGTTTATGCCGGTCAATTTCGAGAGCATCTTCAGCAAGAACGCCCGCACGGCGCCGGACAAGCTCACGTTCGGCGATATCTGGCGGATGACCGAAGGCCAAAGGGTGGCGCTCGACTTGCTTGGGAGGATCGCGAGTAAGGGGGAGTGGATATTGCTCTACGTGCTTGCGAAAGATGAGGAAGGATTCCTCAGGAAGGAGGCTGTTCGCCGCTGCTTCGATGGGAGCCTATTCGAGTCGATTGCCCAGCAGAGAAGGGAGGCACATGAGAAGCAGAAGTAG
  • >LOC_Os04g43200.2
    ATGGCGGAGGAGGCGGCTAGCAAGGCAGCGCCGACCGATGCGCTGTCGTCCGTGGCGGCGGAGGCGCCGGTGACGAGAGAACGGCCGGTCCGAGCGGACTTGGAAGTGCAGATTCCGAAGCCCTATTTGGCCCGAGCTCTGGTTGCTCCGGACGTGTACCATCCTGAAGGAACCGAGGGGCGTGACCACCGGCAGATGAGTGTGCTGCAGCAGCATGTGGCTTTCTTCGACCTGGATGGCGACGGTATCGTTTATCCATGGGAAACTTATGGAGGACTACGGGAATTGGGCTTCAACGTGATTGTTTCGTTCTTTTTGGCGATAGCCATAAACGTTGGTCTAAGCTACCCAACTCTGCCAAGCTGGATACCATCTCTCCTGTTCCCTATACACATAAAAAACATCCACAGGGCTAAGCACGGCAGCGATAGCTCGACGTACGACAACGAGGGAAGGTTTATGCCGGTCAATTTCGAGAGCATCTTCAGCAAGAACGCCCGCACGGCGCCGGACAAGCTCACGTTCGGCGATATCTGGCGGATGACCGAAGGCCAAAGGGTGGCGCTCGACTTGCTTGGGAGGATCGCGAGTAAGGGGGAGTGGATATTGCTCTACGTGCTTGCGAAAGATGAGGAAGGATTCCTCAGGAAGGAGGCTGTTCGCCGCTGCTTCGATGGGAGCCTATTCGAGTCGATTGCCCAGCAGAGAAGGGAGGCACATGAGAAGCAGAAGTAG
Protein Sequence
  • >LOC_Os04g43200.1
    MAEEAASKAAPTDALSSVAAEAPVTRERPVRADLEVQIPKPYLARALVAPDVYHPEGTEGRDHRQMSVLQQHVAFFDLDGDGIVYPWETYGGLRELGFNVIVSFFLAIAINVGLSYPTLPSWIPSLLFPIHIKNIHRAKHGSDSSTYDNEGRFMPVNFESIFSKNARTAPDKLTFGDIWRMTEGQRVALDLLGRIASKGEWILLYVLAKDEEGFLRKEAVRRCFDGSLFESIAQQRREAHEKQK*
  • >LOC_Os04g43200.2
    MAEEAASKAAPTDALSSVAAEAPVTRERPVRADLEVQIPKPYLARALVAPDVYHPEGTEGRDHRQMSVLQQHVAFFDLDGDGIVYPWETYGGLRELGFNVIVSFFLAIAINVGLSYPTLPSWIPSLLFPIHIKNIHRAKHGSDSSTYDNEGRFMPVNFESIFSKNARTAPDKLTFGDIWRMTEGQRVALDLLGRIASKGEWILLYVLAKDEEGFLRKEAVRRCFDGSLFESIAQQRREAHEKQK*