Gene Details:

Functional Descriptions:

  • Interestingly, the basal expression levels of QSH1 and SHAT1 genes (transcription factors that regulate seed-pod shattering in rice) were significantly lower in these plants than in wild-type plants; however, nitrosative stress negatively regulated the expression of QSH1 and SHAT1 in both WT and RHS1 plants, but positively regulated QSH4 expression in RHS1 plants alone.
  • The absence of RHS1, which encodes a putative armadillo/beta-catenin repeat family protein, resulted in high sensitivity of the RHS1 plants to nitrosative stress.
  • The expression of CPL1 (a negative regulator of seed shattering in rice) was significantly lower in RHS1 plants, and we found that CPL1 expression was correlated with S-nitrosothiol (SNO) alteration in RHS1.
  • Interestingly noe1, a rice mutant with high SNO levels, exhibited low seed shattering, whereas RHS1 resulted in low SNO levels with high seed shattering.
  • A Novel RHS1 Locus in Rice Attributes Seed-Pod Shattering by the Regulation of Endogenous S-Nitrosothiols.
  • The knockout mutant line RHS1 exhibited a significantly high shattering of grains in comparison to the wild-type plants.
  • Therefore, RHS1 is a novel gene that negatively regulates the shattering trait in rice via regulation of endogenous SNO levels.

Literature:

Gene Resources:

  • NCBI ID:
  • UniProt accessions:

Sequences:

cDNA Sequence
  • >LOC_Os04g41250.1
    TCTCCCAAAACAAATCAATTTCCTTCCTCCCGACCGAGCAAGGGGGCGAAAGGACGAGCTACGGACCGAGCCATGGAGGAATTCAAGGCTCGCTCCTTCGTCGCCTTGTCTCCCTCGGGTGAAATTTCATCAATATAGGAAAATAGATTATACCATCAGTTTTTGCACACGCGGATGAAATTTGACTAGGAGAGGCACCATGGAGAAAGTCGAGGTCGATGGGAACTTCATTGGTACGGGTAATTGGAAGCTGCATGGAGCACTATGTAAGCAACTTCACAAAGTAGTTCTTGAAGTATTGGATGTTATTCCTGTCCTTGAAGCTACAAGGCCTGGAAACAGTTCTGGACTTCTTGCATTGAGTTCCTTGCGCATAGCTGTTGAGAAGGCCAAGAATCTACTTCAGTATTGCTCAGAGTGCAGCAAGCTATACTTGGCCTTTACAGCAGAAAATGTCCTGGCAAAATTTGAGAAAGCAAGATATGCGCTTCTGGAAAGTCTTCACCAATTGGAAGAAACACTTCCAGAAGCAGCCAGTTCTCAGATACTAGATATCGCAAAAGATCTGGAGAAAGCAGTTTTTACCTTGGATCTGATTGAAAAGCAAGCTGGTGTGGATGTGAATCAATTAGTTCAGAATGAGGCAAAATCCAATGGCTTTCTTCATGACAATGAACTTGAATTTTTCCGACAAACTGCATTTCGGGTCGGCGTTGCGTCATCGGCAACAGCTCTTACCGAGAGAAGAGCACTTAGAAGGCTTCTTGAGAGGGCTCATGCAGAGGAAGACATAAAGAAAGAATCAGTTGCATCTTATCTACTGCACCTAATGAGGAAATACTCAAGCATTTTTAGGAGCGAAACGACGGATTTCACCAACACATCCATGTGCTCAAGCCCTTCTTGCTCTTCCCGTTCATTGTCCAGCTCGATTGATCTCCATGGAAATGGTCATGTTATTGAAAAGTCGATATCTAGGGTTGGCTCCTTCAATCTCAGGCAGATCAAAGGCTTATCAGGTAGCATGCCATTGCCACCTGAGGAACTAAGGTGCCCGATCTCCTTGCAACTCATGCATGACCCTGTTATTATCGCATCTGGTCAGACATATGAGCGGGCTTGCATTGAGAAATGGTTTAGCAGCGGCAACACAACCTGCCCAAAGACTCGGAATGAATTATCCCAGCTCTCCATGACGCCCAACTATTGCATCAAGGGTTTGATAGCTTCATGGTGCGAGCAGAACGGGGTTCTAGTTCCATCGGCGCCGCCTGATTCTCCCAAGCTCAAGTACCTGAGGATATCATCCTTGAATAGTAGCAAATGTCTTGTGACCAATGGCGTTAGCACGGTCTTGTTTGAAGACACATGCGCGGAGGATGATATTAAGGATGGTGGTAAAGTTGCTTCGGAGGAGTGCACTCGGCAGAACTCCGGAGAGGCTCCATCTGAGATATGTGAAGTTGATCAGGCCTCACCAGAAAAACATCCTCATGAGAACTCCGAGAAGGTAGCAGAGGCGACATGTGAGCTTTGGCTGCGTGTGCTGAGCAAGGACGACGATGAGTGTGTCGACGAGCAACGTGAGGTGATCGAGCAGATTAGATTCCTTTTGAAGGATGACAACGAGCTTAGGAAATACGCGGGTGCTAATGGTATTACCGAGCTACTCATCCACTTTGTGAAGAAGGCAGTCTGCAGAGATGATGTTCAGTGCCAGGTGGTTGGAACAATGGCTCTGTTCAACCTTGCTGTCAGCAATGATAGGAACAAGAAACAACTTCTCTCAGGTGGAGTCCTCCCCTTGATGGAGCAGATGATACAGAAGCCTGAAACCTACGAGGCTGCCGTCGCAATGTACCTGAACATTTCCTGCCTCGCCGAGGCGCAGGCGATCATCGGGCAGAGCGAGGCCGCGCCTCTCCTGATCAAGGGCCTGCAAGGAGACGGCTTCCGGATGAGCAAGACCTGCTGCCTCGACGCTCTCCTGACGCTGTACAACCTGTCGCTGCAATCGTCAAACATCCCGACCCTCATCTCCTCCGGCATCATGCAGAGCCTCCACGACGTCCTGACGCCGTCCTCCCCGACGACGGAGAAGGCCCTGGCCGTGCTGATCAACTTGGCGCTGACCCGCGCCGGCAAGAAGGAGATCATGGCCGACTCGGACATGGTGGGCGCCATCGTCGTCATCCTCGAGAACGGGGACCCCGCCGAGAAGGAGAAGGCCGTGTCGTGCCTGTGGATCATCTGCAGCGGCGATGACGGCGGCAGCCAGATGGTGCTCCAGGAAGGCGTGATCCCGGCGCTGGTGTCCCTGACGGCCAACGGCACGGGCAAGACGAAGGATAAGGCGCAGAGGCTGCTCCTGCTGTTCCGCGGGAAGCGGCAGCGGGAGGTCGAGCAGCTGCAGCCAAGGGTGCAGCTGCACGAGGTGGTGAGCCAGGCAACCGCGCAGCACGAGCAGCAGCAGCAGCAGCAACAAGAAGAAAGCAGCGAGCCAGGCAGCGACAAGATGTCGCGGCTGCGCAATTCAAAGTCGAAGTTGAGAAGGTTCACGAGGGCCCTGGCTAGGTTGCTCAAGAAATGGGGCATCCGCTGATCTGATCTACTACAGAGGAAAGAACCACGTCGGCTAGTGTGAACTTTGGCAAATGCAATATCAATGTTATCTTCCCGTGTGGGTTGACTCACTCGTTTTCGTCTCCTTCGCATTGCCGCGATATCCATACTCGTCTCACTTATGCGGGCATTGACCGTCTCTCTGTACCTATTAACCTTGTGCTAAGGCAAAATTAGTAAAAAGGGTTTAAGCTTATTGTGTTTTGCCAAAATGTAAGCCAAGATGTGTTAACAAGGATTAAGTGCGATCATCAAAGTGTGTAACAAGGATTAATTCAGGTCATAAATCTAATTATATAATACGTAACCCAAATTAA
CDS Sequence
  • >LOC_Os04g41250.1
    ATGGAGAAAGTCGAGGTCGATGGGAACTTCATTGGTACGGGTAATTGGAAGCTGCATGGAGCACTATGTAAGCAACTTCACAAAGTAGTTCTTGAAGTATTGGATGTTATTCCTGTCCTTGAAGCTACAAGGCCTGGAAACAGTTCTGGACTTCTTGCATTGAGTTCCTTGCGCATAGCTGTTGAGAAGGCCAAGAATCTACTTCAGTATTGCTCAGAGTGCAGCAAGCTATACTTGGCCTTTACAGCAGAAAATGTCCTGGCAAAATTTGAGAAAGCAAGATATGCGCTTCTGGAAAGTCTTCACCAATTGGAAGAAACACTTCCAGAAGCAGCCAGTTCTCAGATACTAGATATCGCAAAAGATCTGGAGAAAGCAGTTTTTACCTTGGATCTGATTGAAAAGCAAGCTGGTGTGGATGTGAATCAATTAGTTCAGAATGAGGCAAAATCCAATGGCTTTCTTCATGACAATGAACTTGAATTTTTCCGACAAACTGCATTTCGGGTCGGCGTTGCGTCATCGGCAACAGCTCTTACCGAGAGAAGAGCACTTAGAAGGCTTCTTGAGAGGGCTCATGCAGAGGAAGACATAAAGAAAGAATCAGTTGCATCTTATCTACTGCACCTAATGAGGAAATACTCAAGCATTTTTAGGAGCGAAACGACGGATTTCACCAACACATCCATGTGCTCAAGCCCTTCTTGCTCTTCCCGTTCATTGTCCAGCTCGATTGATCTCCATGGAAATGGTCATGTTATTGAAAAGTCGATATCTAGGGTTGGCTCCTTCAATCTCAGGCAGATCAAAGGCTTATCAGGTAGCATGCCATTGCCACCTGAGGAACTAAGGTGCCCGATCTCCTTGCAACTCATGCATGACCCTGTTATTATCGCATCTGGTCAGACATATGAGCGGGCTTGCATTGAGAAATGGTTTAGCAGCGGCAACACAACCTGCCCAAAGACTCGGAATGAATTATCCCAGCTCTCCATGACGCCCAACTATTGCATCAAGGGTTTGATAGCTTCATGGTGCGAGCAGAACGGGGTTCTAGTTCCATCGGCGCCGCCTGATTCTCCCAAGCTCAAGTACCTGAGGATATCATCCTTGAATAGTAGCAAATGTCTTGTGACCAATGGCGTTAGCACGGTCTTGTTTGAAGACACATGCGCGGAGGATGATATTAAGGATGGTGGTAAAGTTGCTTCGGAGGAGTGCACTCGGCAGAACTCCGGAGAGGCTCCATCTGAGATATGTGAAGTTGATCAGGCCTCACCAGAAAAACATCCTCATGAGAACTCCGAGAAGGTAGCAGAGGCGACATGTGAGCTTTGGCTGCGTGTGCTGAGCAAGGACGACGATGAGTGTGTCGACGAGCAACGTGAGGTGATCGAGCAGATTAGATTCCTTTTGAAGGATGACAACGAGCTTAGGAAATACGCGGGTGCTAATGGTATTACCGAGCTACTCATCCACTTTGTGAAGAAGGCAGTCTGCAGAGATGATGTTCAGTGCCAGGTGGTTGGAACAATGGCTCTGTTCAACCTTGCTGTCAGCAATGATAGGAACAAGAAACAACTTCTCTCAGGTGGAGTCCTCCCCTTGATGGAGCAGATGATACAGAAGCCTGAAACCTACGAGGCTGCCGTCGCAATGTACCTGAACATTTCCTGCCTCGCCGAGGCGCAGGCGATCATCGGGCAGAGCGAGGCCGCGCCTCTCCTGATCAAGGGCCTGCAAGGAGACGGCTTCCGGATGAGCAAGACCTGCTGCCTCGACGCTCTCCTGACGCTGTACAACCTGTCGCTGCAATCGTCAAACATCCCGACCCTCATCTCCTCCGGCATCATGCAGAGCCTCCACGACGTCCTGACGCCGTCCTCCCCGACGACGGAGAAGGCCCTGGCCGTGCTGATCAACTTGGCGCTGACCCGCGCCGGCAAGAAGGAGATCATGGCCGACTCGGACATGGTGGGCGCCATCGTCGTCATCCTCGAGAACGGGGACCCCGCCGAGAAGGAGAAGGCCGTGTCGTGCCTGTGGATCATCTGCAGCGGCGATGACGGCGGCAGCCAGATGGTGCTCCAGGAAGGCGTGATCCCGGCGCTGGTGTCCCTGACGGCCAACGGCACGGGCAAGACGAAGGATAAGGCGCAGAGGCTGCTCCTGCTGTTCCGCGGGAAGCGGCAGCGGGAGGTCGAGCAGCTGCAGCCAAGGGTGCAGCTGCACGAGGTGGTGAGCCAGGCAACCGCGCAGCACGAGCAGCAGCAGCAGCAGCAACAAGAAGAAAGCAGCGAGCCAGGCAGCGACAAGATGTCGCGGCTGCGCAATTCAAAGTCGAAGTTGAGAAGGTTCACGAGGGCCCTGGCTAGGTTGCTCAAGAAATGGGGCATCCGCTGA
Protein Sequence
  • >LOC_Os04g41250.1
    MEKVEVDGNFIGTGNWKLHGALCKQLHKVVLEVLDVIPVLEATRPGNSSGLLALSSLRIAVEKAKNLLQYCSECSKLYLAFTAENVLAKFEKARYALLESLHQLEETLPEAASSQILDIAKDLEKAVFTLDLIEKQAGVDVNQLVQNEAKSNGFLHDNELEFFRQTAFRVGVASSATALTERRALRRLLERAHAEEDIKKESVASYLLHLMRKYSSIFRSETTDFTNTSMCSSPSCSSRSLSSSIDLHGNGHVIEKSISRVGSFNLRQIKGLSGSMPLPPEELRCPISLQLMHDPVIIASGQTYERACIEKWFSSGNTTCPKTRNELSQLSMTPNYCIKGLIASWCEQNGVLVPSAPPDSPKLKYLRISSLNSSKCLVTNGVSTVLFEDTCAEDDIKDGGKVASEECTRQNSGEAPSEICEVDQASPEKHPHENSEKVAEATCELWLRVLSKDDDECVDEQREVIEQIRFLLKDDNELRKYAGANGITELLIHFVKKAVCRDDVQCQVVGTMALFNLAVSNDRNKKQLLSGGVLPLMEQMIQKPETYEAAVAMYLNISCLAEAQAIIGQSEAAPLLIKGLQGDGFRMSKTCCLDALLTLYNLSLQSSNIPTLISSGIMQSLHDVLTPSSPTTEKALAVLINLALTRAGKKEIMADSDMVGAIVVILENGDPAEKEKAVSCLWIICSGDDGGSQMVLQEGVIPALVSLTANGTGKTKDKAQRLLLLFRGKRQREVEQLQPRVQLHEVVSQATAQHEQQQQQQQEESSEPGSDKMSRLRNSKSKLRRFTRALARLLKKWGIR*